scholarly journals Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

2020 ◽  
Vol 44 (4) ◽  
Author(s):  
Rony Marsyal Kunda ◽  
Slamet Diah Volkandari ◽  
Maman Rumanta ◽  
Pieter Kakisina
Keyword(s):  
Growth Hormone ◽  
Polymorphic Information Content ◽  
Growth Trait ◽  
Hair Follicles ◽  
Nonrandom Mating ◽  
Economic Trait ◽  
Goat Population ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Dna Fragment

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.

scholarly journals Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle

2017 ◽  
Vol 42 (3) ◽  
pp. 153 ◽  
Author(s):  
P. P. Agung ◽  
S. Anwar ◽  
W. P. B. Putra ◽  
M. S. A. Zein ◽  
A. S. Wulandari ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gene Polymorphism ◽  
Fragment Length Polymorphism ◽  
Growth Parameters ◽  
Rflp Analysis ◽  
Cattle Breeding ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Dressing Percentage

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.

Sequence analysis and PCR-RFLP of growth hormone (GH) gene in Vembur and Kilakarsal breeds of sheep

2017 ◽  
Vol 23 (2) ◽  
pp. 148
Author(s):  
M. Seevagan ◽  
V. Jeichitra ◽  
R. Rajendran ◽  
K.G. Tirumurugaan
Keyword(s):  
Growth Hormone ◽  
Sequence Analysis ◽  
Pcr Rflp ◽  
Gh Gene

scholarly journals Genetic Polymorphism of Growth Hormone Gene Exon-4 in Surti and Mehsani Goats by PCR- RFLP

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Jyotishree Bayan ◽  
Vishnu Kharadi ◽  
Umed Ramani ◽  
Mamta Janmeda ◽  
Kuldeep Tyagi ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Fragment Length Polymorphism ◽  
Allelic Frequency ◽  
Growth Hormone Gene ◽  
Chain Reaction ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Exon 4 ◽  
Gene Exon

The present investigation was planned to study growth hormone (GH) gene exon-4 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon-4 region was found to be monomorphic on restriction digestion with HaeIII, which revealed only one genotype CC in both Surti and Mehsani goat breeds. The allelic frequency of C was 1.00 in both the breeds of goats with absence of D allele.

scholarly journals Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

2017 ◽  
Vol 17 (4) ◽  
pp. 1053-1062 ◽  
Author(s):  
Mehmet Akif Konca ◽  
Bilal Akyüz
Keyword(s):  
Growth Hormone ◽  
Water Buffalo ◽  
Pcr Amplification ◽  
Chi Square ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Pcr Products ◽  
Chi Square Test ◽  
Prl Gene ◽  
Prolactin Hormone

AbstractThe purpose of this work was to identify GHRH, GH and PRL gene polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method. A total of 126 buffalo were included in this study. PCR amplification gave a 451 bp band for the GHRH gene, a 221 bp band for the GH gene and a 156 bp band for the PRL gene. The PCR products were digested by HaeIII for the GHRH gene, AluI for the GH gene and RsaI for the PRL gene. The GH/AluI and PRL/RsaI polymorphisms were found to be polymorphic, while the GHRH/HaeIII polymorphism was not found in Anatolian water buffalo. The frequencies of GH-L (0.87) and PRL-A (0.55) alleles were found to be high in the examined Anatolian water buffalo. The chi-square test showed that the Anatolian water buffalo were in Hardy-Weinberg (HW) equilibrium for the GH gene while significant deviation was observed from HW equilibrium for the PRL gene. The present study is the first to examine GHRH/HaeIII, GH/AluI and PRL/RsaI polymorphisms in Anatolian water buffalo.

scholarly journals Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

2018 ◽  
Vol 43 (1) ◽  
pp. 1 ◽  
Author(s):  
T. Hartatik ◽  
D. E. Putra ◽  
S. D. Volkandari ◽  
T. Kanazawa ◽  
S. Sumadi
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Body Size ◽  
Growth Traits ◽  
Selection Process ◽  
Growth Trait ◽  
Local Environment ◽  
Growth Hormone Gene ◽  
Pcr Rflp ◽  
Size Of Animals

Growth hormone gene that controls metabolism and body size of animals and polymorphism of the gene is known related to performance of livestock. Identification of gene polymorphisms was important to get early information to determine genetic markers associated with economically desirable traits. Genetic mapping is one of important characterizations in the selection process. Pesisir cattle is one of local cattle in Indonesia that are reared in West Sumatera and have become adaptive to local environment. The present study aimed to identify the genotype of Growth Hormone gene (GH891│MspI) of the Pesisir cattle and crossbred Simmental-Pesisir cattle (designated as SimPes) and relationship between growth performance (body weight and body size). The present study was conducted to 30 blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight and body size were measured at 12-18 months of age. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in this research. The results showed that there exist three variant genotypes (MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI- and MspI+ were 53.3% and 46.7%, respectively. Non-significant effect has found between GH’s genotype and growth traits (Body measurement and body weight) in both of breed cattle. In conclusion, three variant genotypes (GH891│MspI) in Pesisir and SimPes (Simmental x Pesisir) cattle population were found and no significant effect on growth trait performance.

scholarly journals PROFIL GEN GROWTH HORMONE (GH) SAPI HASIL PERSILANGAN MADURA DAN LIMOUSIN DENGAN METODE PCR-RFLP

2020 ◽  
Vol 8 (1) ◽  
pp. 43
Author(s):  
Ghea Aquatica Puteri ◽  
Budi Utomo S ◽  
Roesno Darsono
Keyword(s):  
Growth Hormone ◽  
Dna Extraction ◽  
Fragment Length ◽  
Base Pair ◽  
Gene Profile ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Base Pair Fragment ◽  
Cattle Blood ◽  
Pair Fragment

The purpose of this research was to find out the Growth Hormone (GH) gene profile of the cross breeding between Madura cattle and Limousin cattle (Madrasin). Sampl in the form of cattle blood for this research was obtained from 14 Madrasin cattles in the area of Bangkalan, Madura, East Java. DNA extraction was performed then to provide the result for PCR RFLP, which then indicated that Madrasin cattle’s GH gene profile has 432 base pair fragment length and the RFLP result indicated that Madrasin cattle’s GH genetic was cut off into 180 base pair, 250 base pair, 300 base pair, and 400 base pair. Moreover, there was no V genetic to be found in GH genetic of Madura cattle.

scholarly journals Polymorphism of Insulin-induced gene 1 (INSIG1) of three local beef cattle in Indonesia

2021 ◽  
Vol 888 (1) ◽  
pp. 012026
Author(s):  
S D Volkandari ◽  
E T Margawati ◽  
A S Wulandari ◽  
Indriawati
Keyword(s):  
Beef Cattle ◽  
Regulatory Element ◽  
Growth Trait ◽  
Weinberg Equilibrium ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Sterol Regulatory Element ◽  
Dna Fragment ◽  
Genotype And Allele Frequencies

Abstract Insulin-induced gene 1 (INSIG1) encoded protein that blocked of proteolysis activity from protein sterol regulatory element binding proteins (SREBP). The role gene plays of cholesterol, fatty acid, and glucose metabolism. Indonesia has many several beef cattle which has good quality of growth trait. The aim of this study was to identify polymorphism of INSIG1 gene (SNP 4366 (A>G) and 4534 (T>C)) of three local beef cattle in Indonesia (Bali, Pasundan and Ongole Decendent). One hundred and twenty seven samples were used in this study consisting of Bali cattle (46) from Pulukan Bali Island, Pasundan (36) from UPTD BPPT Beef cattle Ciamis West Java and Ongole Grade (OD) (45). DNA was extracted from whole blood using High Salt method then genotyping used PCR-RFLP method with RsaI and TaqI enzymes. Parameters in this study were genotype and allele frequencies, heterozigosity observed (Ho), expected (He), Hardy-Weinberg Equilibrium (HWE) and Polymorphism Information Content (PIC). Result showed that a 428 bp of DNA fragment was successfully amplified and digested. Three variant of genotypes with two alleles were identified. For SNP 4366 (A>G) were AA, AG and GG with dominant of G allele while SNP 4534 (T>C) were CC, CT and TT with dominant C allele. Both of SNPs in each of breed populations was in Hardy-Weinberg Equilibrium and polymorphic. Additionally, PIC value reached moderate. In conclusion, polymorphism was found in beef cattle and it could be early study for genetic diversity

scholarly journals Growth Hormone Gene Polymorphism and its Relation to Quail Growth and Carcass

2021 ◽  
Vol 9 (8) ◽  
pp. 1395-1397
Author(s):  
Fatma İlhan
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Gene Polymorphism ◽  
Restriction Enzyme ◽  
Carcass Traits ◽  
Growth Hormone Gene ◽  
Japanese Quails ◽  
Intron 1 ◽  
Pcr Rflp ◽  
Gh Gene

In this study, it was aimed to determine the polymorphism of GH (growth hormone) gene in Japanese quails and the relationships between these genes and body weight and carcass traits. 3 genotypes (AA, AB and BB), 2 (A and B) alleles were detected by cutting the GH intron 1 region with restriction enzyme MspI. As a result of variance analysis, it was determined that the hatching weights of the animals with B allele and liver weights were higher. Thus, it is seen that GH gene and PCR-RFLP technique can be used in breeding studies.

Growth hormone and somatostatin gene expression in pituitary adenomas with active acromegaly and minimal plasma growth hormone elevation

1990 ◽  
Vol 122 (6) ◽  
pp. 745-752 ◽  
Author(s):  
Patrick Pagesy ◽  
Jacques Y. Li ◽  
Françoise Rentier-Delrue ◽  
Olivier Delalande ◽  
Yves Le Bouc ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Northern Blot Analysis ◽  
Secretory Activity ◽  
Ultrastructural Level ◽  
Wide Range ◽  
Igf I ◽  
Gh Gene ◽  
Basal Plasma ◽  
Active Acromegaly ◽  
Plasma Gh

Abstract. Some patients with active acromegaly have elevated plasma IGF-I concentrations with only minimal elevation of plasma GH. We compared adenomatous GH and SRIH expression in 3 such patients (patients No. 1, 2 and 3; basal plasma GH level < 4 μg/l) and in 3 acromegalic patients with high basal plasma GH level (patients No. 4, 5 and 6; 51.7 ± 16.1 μg/l, mean ± sem). By immunocytochemistry, all the tumours proved to be somatotropic adenomas. At the ultrastructural level, signs of low secretory activity were observed in adenomas from patients No. 2 and 3. Perifused adenoma cells of patients No. 1, 2 and 3 released very little GH compared with those of patients No. 4, 5 and 6 (1± 0.37 vs 51.5± 34.1 μg · (10−6 cells) · min−1, p< 0.001). Adenoma SRIH content was 65.7 and 30.6 pg/mg proteins in patients No. 1 and 2, whereas it was undetectable in the others (patients No. 4, 5 and 6). Northern blot analysis showed that the GH gene was poorly expressed in the adenomas from patients No. 1, 2 and 3 compared with the adenomas from patients No. 4, 5 and 6. SRIH mRNA was detected in all 6 adenomas. However, the signal was more intense in the adenomas from patients No. 1, 2 and 3 than in those from patients No. 4, 5 and 6. In conclusion, because of the variability of the biosynthetic and secretory potential of the somatotropic adenomas, patients harbouring this type of pituitary tumours can exhibit a wide range of plasma GH levels. In acromegaly with minimal elevation of plasma GH, the synthesis of SRIH by the adenoma cells themselves could play a role in the inhibition of GH expression.

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