scholarly journals Prevalence of Staphylococcus aureus in Lactating Cows with Subclinical Mastitis and their Antibiogram in Organized Dairy Farm, Maharashtra, India

2018 ◽  
Vol 7 (03) ◽  
pp. 3674-3680
Author(s):  
Mahendra Mohan Yadav
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Lactating Cows

scholarly journals Case Study: Misdiagnosis of Nonhemolytic Staphylococcus aureus Isolates from Cases of Bovine Mastitis as Coagulase-Negative Staphylococci

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 252
Author(s):  
Valerie E. Ryman ◽  
Felicia M. Kautz ◽  
Steve C. Nickerson
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Dairy Farm ◽  
Blood Agar ◽  
Coagulase Negative Staphylococci ◽  
Mannitol Salt Agar ◽  
Lactating Cows ◽  
Presumptive Identification ◽  
Microbiological Techniques

Staphylococcus aureus is one of the most concerning mastitis-causing pathogens in dairy cattle. Using basic microbiological techniques, S. aureus is typically identified by colony characteristics and hemolysis on blood agar where isolates without hemolysis are typically considered to be coagulase-negative staphylococci (CNS) isolates. Herein, we present a decade-long case study where suspected S. aureus isolates from one Georgia dairy farm were further tested to confirm presumptive identification. Presumptive identification of bacterial growth from 222 mammary secretions from bred Holstein heifers and lactating cows was conducted at the time of collection. Presumptive identification of S. aureus on blood agar was based on observation of colony morphology, color, and presence or absence of a broad zone of incomplete hemolysis and a smaller zone of complete hemolysis at 48 h. Those without hemolysis were presumptively characterized as CNS. All isolates were further plated on mannitol salt agar and a coagulase test was performed. A positive for both of these tests together was deemed to be S. aureus. A selection of isolates was tested using API® Staph to biochemically confirm S. aureus identification. Data showed that 63.96% of isolates presumed to be CNS isolates were identified as S. aureus, 9.46% of isolates presumed to be CNS isolates were identified as coagulase-positive staphylococci (CPS) species (but not S. aureus), and 26.58% of samples that were presumed to be CNS isolates were identified correctly.

scholarly journals PREVALENCE OF SUBCLINICAL MASTITIS IN DAIRY COWS IN SELECTED AREAS OF BANGLADESH

2012 ◽  
Vol 9 (1) ◽  
pp. 73-78 ◽  
Author(s):  
MA Islam ◽  
MZ Islam ◽  
MA Islam ◽  
MS Rahman ◽  
MT Islam
Keyword(s):  
Dairy Cows ◽  
Rural Areas ◽  
Dairy Farm ◽  
Local Area ◽  
Subclinical Mastitis ◽  
Local Breed ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Late Stages

This research was carried out to determine the prevalence of subclinical mastitis in lactating Dairy Cow of Bangladesh Agricultural University dairy farm (BAUDF) and rural areas of Tangail sadar upazila of Bangladesh during the period of July 2009 to April 2010. A total of 200 milk samples (40 from BAUDF and 160 from Tangail sadar upazila) were collected for this study which were subjected to physical examination and subsequently screened for subclinical mastitis using three indirect tests viz. White Side Test (WST), California Mastitis Test (CMT), and Surf Field Mastitis Test (SFMT). Overall prevalence of subclinical mastitis (SCM) in lactating dairy cows found in this study was 29%. Cows were infected with SCM 29.5%, 27.5% and 25.5% detection by CMT, WST and SFMT respectively. Higher prevalence of SCM was detected in milch crossbred cows (36.36%) in comparison to local bred cows (24.61%) maintained under extensive management system in Rural area of Tangail sadar upazila. The prevalence of SCM was recorded in 31.58%, 30.76% and 68.75% in cows of local area of Tangail sadar upazila, and 25.0%, 40.0% and 71.42% in cows of BAU,DF during the early, mid and late stages of lactation respectively. The highest prevalence of SCM was recorded during the early lactation stage in both the local breed cows (30.0%) and cows of BAUDF (45.83%) in comparison to their respective mid and late stages of lactation. The prevalence of SCM was highest in lactating cows having third lactation and high yielding (cows produced >10 liter milk per day) both in local breed and crossbred cows.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11216 Bangl. J. Vet. Med. (2011). 9(1): 73-78 

scholarly journals Association of selected risk factors with bovine subclinical mastitis

2021 ◽  
Vol 15 (2) ◽  
pp. 153-160
Author(s):  
Nesma Helmy Youssif ◽  
Nagah Mostafa Hafiz ◽  
Mohamady Ahmed Halawa ◽  
Mena Fouad Saad
Keyword(s):  
Risk Factors ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Mean Value ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Holstein Friesian ◽  
Significant Difference ◽  
Parity Number

A total of 695 Holstein Friesian lactating dairy cows were examined for the prevalence of subclinical mastitis (SCM) and its association with selected risk factors in a private dairy farm at Fayoum district, Egypt. 444 lactating cows with 1145 quarters were found positive for SCM with prevalence of 63.88% and 41.18% at cow and quarter level respectively based on California Mastitis Test (CMT), Electrical Conductivity (EC) that had a mean value of 6.27 ± 0.066 mS/cm and Somatic Cell Count (SCC) with a mean value of 8.8×105± 9.2×103 cells/ml. The risk factors investigated in this study revealed that the highest percentage of SCM was observed in winter, in cows at the late lactation stage with a percentage of 32.21 and 59.91 respectively. The results concluded that the young age cows were exposed to SCM more than old ones. While the occurrence of SCM was higher in cows with 2nd, 3rd and 1st than those with 4, 5, 6th parity number. The analytical results also revealed that there is a highly significant difference (p < 0.05) between numbers of parity. However, there is no significant difference (p > 0.05) between different stages of age, lactation and season as risk factors.

scholarly journals Prevalence of sub-clinical mastitis in dairy farms

2014 ◽  
Vol 30 (2) ◽  
pp. 70-77
Author(s):  
MA AL Quaderi ◽  
M Husain ◽  
MGS Alam ◽  
M Khatun ◽  
MA Hossain
Keyword(s):  
Prevalence Rate ◽  
Dairy Farm ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Cattle Breeding ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Breeding Station ◽  
High Prevalence

A total of 560 lactating cows belonging to Bangladesh Agricultural University dairy farm (n = 59): Local Zebu (L), Local × Friesian (L × F), Local × Jersey (L × J), Local × Red Chittagong Cattle (L × RCC), Local × Sahiwal (L × SL), Red Chittagong Cattle (RCC); and Central Cattle Breeding Station and Dairy Farm (n = 501): Local Zebu, L × F, SL × F, Sahiwal and Australian Friesian Sahiwal (AFS) were selected to measure prevalence of subclinical mastitis (SM). The California Mastitis Test was done and the prevalence of SM was 68% on cow basis and 57% on quarter basis. The prevalence rate was significantly higher in L × F (87%, P < 0.05), SL × F (88%, P < 0.05), L × J (100%, P < 0.01), AFS (89%, P< 0.05) and SL (100%, P < 0.01). The local Zebu (31%) and RCC (28%) were least susceptible to SM. SM was significantly higher in multiparous (P < 0.05) and older cows (P < 0.05). The front quarters were more prone to SM than the rear. Factors such as breed, age, parity and management may have been responsible for high prevalence of SM in both farms. DOI: http://dx.doi.org/10.3329/bvet.v30i2.18257 Bangl. vet. 2013. Vol. 30, No. 2, 70-77

scholarly journals Microbiological and molecular aspects of Staphylococcus aureus isolated from bovine mastitis in West-Central Brazil

2020 ◽  
Vol 14 (1) ◽  
pp. 87
Author(s):  
T. B. Vieira ◽  
R. Almeida ◽  
I. B. Jesus ◽  
F. Freitas ◽  
R. T. Kemper ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Enterotoxin Gene ◽  
Central Brazil ◽  
Lactating Cows ◽  
Molecular Aspects ◽  
And Staphylococcus Aureus

Bovine mastitis is a major disease affecting dairy cattle, and Staphylococcus aureus is one of the most important agent involved in this condition due to its capacity to produce enterotoxins and develop resistance to antimicrobial agents. This study aimed to detect S. aureus strains in milk samples from cows with subclinical mastitis employing microbiological and molecular analysis.  Eleven farms were visited and from 187 lactating cows sampled, 33 S. aureus strains were isolated.  Only one of the 33 strains was positive for mecA resistance gene, 23 were positive for sea enterotoxin gene, and none was positive for seb or sec enterotoxin gene. S. aureus strains were submitted to in vitro antimicrobial susceptibility test and 63.6% (21/33) were susceptible to all antimicrobials tested, while 36.3% (12/33) were resistant to one or more antimicrobial agents. Identification of mecA and the sea genes highlighted the need to elaborate strategies to reduce problems related to animal. Furthermore, the identification of bovine mastitis caused by S. aureus is very important to manage herd and to public health, since milk contaminated by this pathogen can lead to serious health problems.

scholarly journals HOST DETERMINANTS BASED COMPARATIVE PREVALENCE OF SUBCLINICAL MASTITIS IN LACTATING HOLSTEIN-FRIESIAN CROSS COWS AND RED CHITTAGONG COWS IN BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 17-21 ◽  
Author(s):  
AFMG Rabbani ◽  
MA Samad
Keyword(s):  
Milk Production ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Severe Reaction ◽  
Lactating Cows ◽  
Host Determinants ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Holstein Friesian

A comparative prevalence of sub-clinical mastitis (SCM) based on host risk factors was studied by using California Mastitis Test (CMT) in 96 Holstein-Friesian cross cows (HFCC) of Abu Baker Dairy Farm at Pansha Upazilla in Rajbari district and 20 Red Chittagong cows (RCC) of Bangladesh Agricultural University Dairy Farm (BAUDF), Mymensingh during the period from January to June 2008. Out of the 116 lactating cows examined, 51 (43.97%) showed positive reactivity to CMT. Similar prevalence rate of SCM was recorded in both the HFCC (43.75%) and RCC (45.0%). The overall prevalence of SCM on the basis of lactation stages showed that all the three lactation stages had SCM but there was a tendency to increase its prevalence rates from early (30.43%), mid (32.26%) to late (69.23%) stages. The highest prevalence of SCM was recorded at late lactation stage in both the HFCC (68.75%) and RCC (71.43%) in comparison to their respective mid and early lactation stages. The parity-wise prevalence of SCM was recorded as 18.42%, 55.0%, 66.67%, 75.0% and 75.0% in HFCC whereas, it was 20.0%, 20.0%, 66.67%, 75.0% and 66.67% in RCC at their 1st, 2nd, 3rd, 4th and 5th parity, respectively. This study recorded a relationship between the increased prevalence of SCM with decreased of milk production in both the groups of lactating cows. The respective prevalence of SCM at high, medium and low level of milk production in HFC (14.29%, 33.33% and 70.59%) and RCC (14.29%, 50.00% and 71.42%) were found significantly (p < 0.01) correlated with milk production. An overall 19.18% quarters was affected with SCM, of which 4.31%, 7.33% and 7.38% showed mild, moderate and severe reaction to CMT. However, comparatively, higher percentage of quarter infection was recorded in HFCC (20.31%) than RCC (13.75%). It may be concluded from these observations that both the HFCC and RCC should be monitored by using CMT for the detection of SCM in order to provide rational treatment and control it to make the dairy farming profitable. DOI = 10.3329/bjvm.v8i1.7397 Bangl. J. Vet. Med. (2010). 8(1): 17-21

MONITORING AND CONTROL OF THE “ANIMAL” SUBSYSTEM IN THE COMPLEX BIOTECHNICAL SYSTEM “MAN-MACHINE-ANIMAL” OF A DAIRY FARM

2020 ◽  
pp. 4-10
Author(s):  
VLADIMIR V. KIRSANOV ◽  
◽  
DMITRIY YU. PAVKIN ◽  
FEDOR E. FEDOR E. VLADIMIROV ◽  
EVGENIY А. NIKITIN ◽  
...  
Keyword(s):  
Dairy Farm ◽  
Animal Husbandry ◽  
Reproductive Organs ◽  
Monitoring And Control ◽  
Lactating Cows ◽  
Research Activities ◽  
Mutual Adaptation ◽  
Biotechnical System ◽  
Complex Project ◽  
And Control

A modern dairy farm is a complex biotechnical “man-machine-animal” system, where purposeful human activity concentrates mainly on the control of the “machine” and “animal” subsystems, thus making the whole system ergatic. Increasing the interaction effi ciency of machine subsystems with biological objects (animals) requires an in-depth study of the properties and characteristics of the latter, their behavior, adaptive and refl ex mechanisms that ensure the mutual adaptation of machine and biological subsystems. The paper considers general functionality of the “animal” subsystem, which includes lists of monitored parameters (functions) in pre-weaning, pre-lactation and lactation periods. In a similar way, functionals of the subsystems of the general musculoskeletal development of the animal’s body, respiratory and digestive organs, comfort of the habitat, development and control of the reproductive organs of lactating cows were obtained accompanied with a list of controlled functions and parameters. To carry out a set of research activities in this fi eld, FSAC VIM is planning to carry out a complex project that will increase the levels of automation, digitalization and intellectualization of animal husbandry, provide for comfortable environment, optimal rediced-impact service modes for animals and their extended productive longevity, increased quality of milk and autonomous functioning of individual local biotechnical subsystems.

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