scholarly journals Plasmid Profile and Plasmid Curing of Some Enterobacteriaceae Resistant to Ampicillin Isolated from Food, Human Stool, Chicken Stool in Ekiti State

2021 ◽  
Vol 10 (01) ◽  
pp. 2344-2362
Author(s):  
O. K. Ekundayo
Keyword(s):  
Plasmid Profile ◽  
Plasmid Curing ◽  
Human Stool

scholarly journals Effects of plasmid curing on Rhizobium spp.

2013 ◽  
Vol 2 (1) ◽  
pp. 34-37 ◽  
Author(s):  
Mukitu Nahar ◽  
Zinat Mahal ◽  
Hasan M Zahid ◽  
Khadiza Zaman ◽  
Fahmida Jahan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Biochemical Analysis ◽  
Profile Analysis ◽  
Plasmid Profile ◽  
Plasmid Curing ◽  
Rhizobial Strain ◽  
Symbiotic Relationships ◽  
Rhizobial Species ◽  
Rhizobium Spp ◽  
The Difference

Present study was carried out to determine the difference between the wild type rhizobial strains and plasmid cured strains for some important characteristics such as antibiotic resistance. The rhizobial strains were isolated from locally produced legumes of different varieties such as Pisum sativum, Sesbania aculeata, Vigna mungo, Phaseolus vulgaris, Lens culinaris and Arachis hypogea.They were purified and subjected to various morphological, cultural and biochemical analysis, and also to antibiogram. All the strains showed resistance against amoxicillin and bacitracin, and exhibited higher (50% or more) resistance against ampicillin, erythromycin, nalidixic acid and strptomycin. The isolates were then subjected to plasmid profile analysis. To determine appropriate dose for plasmid curing, Sesbania nodulating rhizobial strain was treated with different concentrations of acridine orange (plasmid curing agent). Rhizobial growth was found in yeast mannitol broth after curing with 30 ?g/ml of acridine orrange. The rhizobial strain was found to be sensitive against amoxicillin, streptomycin and ampicillin after plasmid curing and no exopolysaccharides were found. The present study reveals that the plasmid in rhizobial species might confer the antibiotic resistance and possibly involve in maintaining symbiotic relationships. DOI: http://dx.doi.org/10.3329/sjm.v2i1.15212 Stamford Journal of Microbiology, Vol.2(1) 2012: 34-37

scholarly journals Molecular typing ofSalmonella entericaserovars Enteritidis, Corvallis, Anatum and Typhimurium from food and human stool samples in Tunisia, 2001–2004

2007 ◽  
Vol 136 (4) ◽  
pp. 468-475 ◽  
Author(s):  
R. BEN AISSA ◽  
N. AL-GALLAS
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Analysis ◽  
Plasmid Analysis ◽  
Serovar Typhimurium ◽  
Stool Samples ◽  
Plasmid Profiling ◽  
Human Stool

SUMMARYDuring the period from 2001 to 2004, a total of 72 isolates ofSalmonella entericaserovars: Anatum (n=40), Enteritidis (n=18), Corvallis (n=8), and Typhimurium (n=6), of various origins (mainly food and diarrhoeagenic stool samples), were collected and further characterized by antibiotic resistance, plasmid analysis, and pulsed-field gel electrophoresis (PFGE). Forty-five isolates presented multidrug resistance to antibiotics. Among which oneS. entericaserovar Anatum isolate was resistant to 11 antibiotics, and oneS. entericaserovar Typhimurium DT104 isolate was resistant to eight antibiotics. Plasmid profiling identified eight plasmid profiles (with 1–5 plasmids) among the isolates, of which one plasmid profile (P01) was predominant.XbaI PFGE analysis revealed the presence of a predominant clone of the four studiedSalmonellaserovars circulating in Tunisia throughout the years 2001–2004.

Hepatitis A Antigen in Liver, Bile and Stool

1975 ◽  
Vol 33 ◽  
pp. 340-341
Author(s):  
D.R. Jackson ◽  
J.H. Hoofnagle ◽  
A.N. Schulman ◽  
J.L. Dienstag ◽  
R.H. Purcell ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Liver Enzymes ◽  
Hepatitis A Virus ◽  
Type A ◽  
Initial Study ◽  
Virus Like Particle ◽  
Elevated Liver Enzymes ◽  
Ag Particles ◽  
Ag Particle ◽  
Human Stool

Using immune electron microscopy Feinstone et. al. demonstrated the presence of a 27 nm virus-like particle in acute-phase stools of patients with viral hepatitis, type A, These hepatitis A antigen (HA Ag) particles were aggregated by convalescent serum from patients with type A hepatitis but not by pre-infection serum. Subsequently Dienstag et. al. and Maynard et. al. produced acute hepatitis in chimpanzees by inoculation with human stool containing HA Ag. During the early acute disease, virus like particles antigenically, morphologically and biophysically identical to the human HA Ag particle were found in chimpanzee stool. Recently Hilleman et. al. have described similar particles in liver and serum of marmosets infected with hepatitis A virus (HAV). We have investigated liver, bile and stool from chimpanzees and marmosets experimentally infected with HAV. In an initial study, a chimpanzee (no.785) inoculated with HA Ag-containing stool developed elevated liver enzymes 21 days after exposure.

scholarly journals Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 188
Author(s):  
Tanja Hoffmann ◽  
Andreas Hahn ◽  
Jaco J. Verweij ◽  
Gérard Leboulle ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Pcr Assay ◽  
Bead Beating ◽  
Stool Samples ◽  
Pcr Assays ◽  
Human Stool

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with group-, genus-, and species-specific real-time PCR assays targeting helminths and nonhelminth pathogens in human stool samples. From 25 different in-house and commercial helminth real-time PCR assays applied to 77 stool samples comprising 67 historic samples and 10 external quality assessment scheme samples positively tested for helminths, higher numbers of positive test results were observed after bead-beating-based nucleic acid extraction for 5/25 (20%) real-time PCR assays irrespective of specificity issues. Lower cycle threshold values were observed for one real-time PCR assay after the standard extraction scheme, and for four assays after the bead-beating-based scheme. Agreement between real-time PCR results after both nucleic acid extraction strategies according to Cohen’s kappa ranged from poor to almost perfect for the different assays. Varying agreement was observed in eight nonhelminth real-time PCR assays applied to 67 historic stool samples. The study indicates highly variable effects of harsh nucleic acid extraction approaches depending on the real-time PCR assay used.

scholarly journals A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study

2021 ◽  
Author(s):  
Michael P Coryell ◽  
Mikhail Iakiviak ◽  
Nicole Pereira ◽  
Pallavi P Murugkar ◽  
Jason Rippe ◽  
...  
Keyword(s):  
Validation Study ◽  
Healthy Human ◽  
Human Stool

scholarly journals Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples

2021 ◽  
Vol 9 (2) ◽  
pp. 209
Author(s):  
Romy Razakandrainibe ◽  
Célia Mérat ◽  
Nathalie Kapel ◽  
Marc Sautour ◽  
Karine Guyot ◽  
...  
Keyword(s):  
Large Scale ◽  
Cryptosporidium Oocyst ◽  
Clinical Importance ◽  
Epidemiological Studies ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cryptosporidium Spp ◽  
Stool Samples ◽  
Conventional Microscopy ◽  
Human Stool

Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting Cryptosporidium spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA Cryptosporidium kit, Savyon Diagnostics, Israel) for the detection of Cryptosporidium spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.

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