scholarly journals Morphological Characterization of Different Rice Genotypes under Drought Stress Conditions

Author(s):  
Ritika Yadav ◽  
Gurdeep Bains
Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.


2016 ◽  
Vol 45 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Susmita Dey ◽  
Jyothi Badri ◽  
V. Prakasam ◽  
V. P. Bhadana ◽  
K. B. Eswari ◽  
...  

2020 ◽  
Author(s):  
Soumya Kumar Sahoo ◽  
Goutam Kumar Dash ◽  
Arti Guhey ◽  
Mirza Jaynul Baig ◽  
Madhusmita Barik ◽  
...  

ABSTRACTRice production is severely threatened by drought stress in Eastern India. To develop drought tolerant varieties, selection of donors for breeding programme is crucial. Twenty one selected rice genotypes including both tolerant and sensitive to drought were grown under well-watered and drought stress conditions in dry seasons of two successive years of 2017 and 2018. Leaf water potential, relative water content displayed significant difference among the genotypes during vegetative screening. At reproductive stage drought screening, days to 50% flowering was delayed in all genotypes except N22 and Anjali (showed early flowering) however grain yield and other yield related traits decreased significantly compared to well watered condition. Correlation analysis of phenological and yield related traits with grain yield revealed that tiller numbers and panicle numbers are highly correlated with grain yield both under well-watered and water stress conditions and contributes maximum towards grain yield. The dendrogram grouped Mahamaya, Sahabhagidhan, Poornima, IBD 1, Hazaridhan, Samleshwari and Danteshwari into one cluster which performed better under water stress conditions and had grain yield more than 1.69 tha−1. Sahabhagidhan, Poornima, Vandana, and N22 displayed tolerance to drought both under vegetative and reproductive conditions which could be a good selection for the breeders to develop drought tolerant rice cultivars for eastern region of India.


2016 ◽  
Vol 3 (2) ◽  
pp. 59-65
Author(s):  
M.M. Emam Ahmed ◽  
◽  
Md. Maksudul Haque ◽  
A.B.M Arif Hasan Khan Robin ◽  
Mohammad Anwar Hossain ◽  
...  

2019 ◽  
Vol 24 (3) ◽  
pp. 305-315
Author(s):  
Pranjali Harischandra Ghodke ◽  
Shiv Ramakrishnan ◽  
Dhananjay V. Shirsat ◽  
Gourav Kumar Vani ◽  
Ajay Arora

2016 ◽  
Vol 21 (2) ◽  
pp. 71
Author(s):  
Untung Susanto ◽  
M. Y. Samaullah ◽  
R. H. Wening ◽  
M. J. Mejaya

<p>Perubahan iklim global menyebabkan meluasnya daerah dengan risiko kekeringan. Cekaman kekeringan pada tanaman padi terutama terjadi di lahan gogo, tadah hujan, dan irigasi terbatas. Perakitan varietas yang cocok untuk daerah-daerah tersebut diharapkan dapat meningkatkan hasil padi secara nyata. Padi umur genjah efektif untuk mengantisipasi cekaman kekeringan fase generatif di akhir musim hujan melalui mekanisme escape. Namun demikian, kekeringan yang tidak dapat diprediksi yang dapat terjadi pada awal, tengah, maupun akhir musim tanam memerlukan varietas yang betul-betul toleran terhadap cekaman kekeringan. Penggabungan sifat umur genjah dan toleran kekeringan diharapkan mampu mengantisipasi kondisi cekaman kekeringan. Penelitian ini bertujuan untuk mengetahui respons 23 genotipe padi umur genjah yang terdiri atas 3 varietas unggul, 10 varietas lokal, dan 10 galur introduksi yang diberi perlakuan optimum, tercekam kekeringan pada fase vegetatif (diairi hingga 7 hari setelah tanam dan diairi kembali ketika cek peka mengering), dan generatif (diairi hingga 28 hari setelah tanam dan diairi kembali hanya jika cek peka mengering). Penelitian dilakukan di Kebun Percobaan BB Padi di Sukamandi pada MK 2009. Genotipe yang diuji diberi perlakuan pengairan tersebut di atas tanpa ulangan untuk mengetahui secara deskriptif kondisi karakter-karakter penting genotipe-genotipe yang diuji pada ketiga kondisi pengairan tersebut. Hasil penelitian menunjukkan bahwa berdasarkan karakter hasil, Indeks Cekaman Kekeringan (IKK; Drought Sensitifity Index, DSS), Nilai Relatif (Relative Values, RV), dan keragaan agronomi, galur OM 1490 teridentifikasi toleran terhadap cekaman kekeringan fase vegetatif dan generatif dengan hasil 28,17 g/tanaman, 24,11 g/tanaman, dan 24,72 g/tanaman pada kondisi optimum, tercekam vegetatif, dan generatif. OM 1490 telah dilepas sebagai varietas Inpari 13 pada tahun 2010 dan varietas ini telah digunakan dalam kegiatan pemuliaan tanaman menghasilkan galur-galur harapan yang baru.</p><p> </p><p><strong>Abstrak</strong></p><p>Global climate change has caused the spread of drought prone areas. Drought stress for rice mostly happen in upland, rainfed, and limited irrigation lowland areas. Development of varieties suitable for those areas would significantly increase rice yield. Early maturing genotypes are useful to anticipate predictable early or terminal drought by escape mechanism. Nevertheless, unpredictable drought happening during early, mid season and final planting season results in the need of genuinely droughttolerant genotypes. Combining early maturing and drought tolerant seems to be highly powerful to anticipate drought. This research was aimed to study the response of 23 early maturing rice genotypes consisting of 3 released varieties, 10 local varieties, and 10 introduced lines. The genotypes were exposed to vegetative (watered until 7 days after transplanting and rewater when the susceptible check was drying) and generative phase (watered until 28 days after transplanting and rewatered only if susceptible check was drying), drought stress conditions, and also optimum condition. The experiment was conducted in ICRR Experimental Station in Sukamandi during Dry Season (DS) of 2009. The genotypes were treated with three watering conditions mentioned above without replication to initially see the descriptive statistics of some agronomic traits of the genotypes under the mentioned conditions. The results showed that based on yield, Drought Sensitifity Index (DSS), Relative Values (RV), and agronomic performance, OM 1490 had been identified as tolerant to vegetative and generative drought stresses. It yielded 28.17 g/plant, 24.11 g/plant, and 24.72 g/plant at optimum, vegetative, and generative drought condition, respectively. OM 1490 had been released as Inpari 13 in 2010 and it had beed utilized for further breeding effort resulting new promising lines.</p>


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


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