scholarly journals Establishing and Quantifying the Phosphate Solubilizing Potential of the Brinjal Bacterial Isolate

2021 ◽  
Vol 10 (11) ◽  
pp. 83-88
Author(s):  
C. Jenifer Lolita A. C. Manjula ◽  
E. Keshamma
Keyword(s):  
Plant Growth ◽  
Bacterial Isolate ◽  
Nitrogenase Activity ◽  
Solanum Melongena ◽  
Steady Increase ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Beneficial Effects ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing

Rhizosphere, phylloplane and caulosphere is the region where a complex community of microbes, mainly bacteria and fungi are present. The microbe plant interaction in these regions can be beneficial, neutral, variable, or deleterious for plant growth. The bacteria that exert beneficial effects on plant development are termed plant growth promoting bacteria. To quantify the amount of phosphate solubilizing bacteria from rhizosphere, phylloplane and caulosphere of brinjal (Solanum melongena L.). Materials and methods: Brinjal (Solanum melongena L.) plants of different varieties were collected from seven locations around Bangalore viz., Hessaraghatta, Yelahanka, Kengeri, Madi vala, Hebbal, Tirumalapura and Attibele were also screened for the presence of phosphate solubilizing bacteria. Nitrogenase activity was estimated by acetylene reduction assay and analyzed by gas chromatography. The amount of nitrogen fixed brinjal bacterial isolate was quantified by micro Kjeldhal method. The amount of nitrogen fixed by the BBI was equivalent to 23.5 nm of C2H2 reduced/tube/hour. The amount of nitrogen fixed by the BBI showed a steady increase upto three days (75 nm of C2H2 reduced/tube/hour) after which there was a decline in the amount of nitrogen fixed by the microbe. Phosphate solubilization by the bacteria isolated from brinjal is highly beneficial to the crop, as it would always make more phosphate available to the crop. This phosphate solubilizing potential could be harnessed to reduce the input of inorganic fertilizers. For the first time the presence of phosphate solubilizing bacteria on the rhizosphere and endorhizosphere of brinjal (Solanum Melongena L.) cultivars was established.

scholarly journals Isolation of Bacteria from Rhizosphere, Phylloplane and Caulosphere of Brinjal (Solanum melongena L.)

2021 ◽  
Vol 8 (6) ◽  
pp. 15-19
Author(s):  
Jennifer Lolitha C. ◽  
Manjula A. C. ◽  
Prathibha K. Y. ◽  
Keshamma E.
Keyword(s):  
Plant Growth ◽  
Solanum Melongena ◽  
Growth Media ◽  
Plant Growth Promoting Bacteria ◽  
Vegetable Crops ◽  
Beneficial Effects ◽  
Moderate Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Growth Of Bacteria

Rhizosphere, phylloplane and caulosphere is the region where a complex community of microbes, mainly bacteria and fungi are present. The microbe- plant interaction in these regions can be beneficial, neutral, variable, or deleterious for plant growth. The bacteria that exert beneficial effects on plant development are termed plant growth promoting bacteria. To isolate the bacteria from rhizosphere, phylloplane and caulosphere of brinjal (Solanum melongena L.). The seeds of 16 cultivars of brinjal (Solanum melongena L.) viz., Arka keshav, Arka shirish, Arka kusumaker, and IIHR accession numbers 389,386,387,377 Tc, BB44, 391, 433, 434, 427, 447, 448, 476 and 487 that were used in the initial screening experiment were obtained from the Department of Vegetable crops, IIHR, Hessaraghatta, Bangalore. Brinjal (Solanum melongena L.) plants of different varieties were collected from seven locations around Bangalore viz., Hessaraghatta, Yelahanka, Kengeri, Madi vala, Hebbal, Tirumalapura and Attibele were also screened for the presence of associative bacteria. Associative microorganisms isolated from the rhizosphere, phylloplane and shoot regions of brinjal (Solanum melongena L.), revealed the presence of three morphologically different colonies. 80% of 16 cultivars of the brinjal (Solanum melongena L.) screened showed the presence of associative bacterial colonies. In this study diazotrophic BBI were obtained from the rhizoplane, phylloplane and stem of 16 cultivars of brinjal (Solanum melongena L.) that were screened. The dominant pearl-colored colonies isolated from all varieties of brinjal plants that were screened was identified and showed maximum nitrogen fixing ability compared with that of the other colonies. The phylloplane of brinjal (Solanum melongena L.) plants from seven different locations around Bangalore showed the presence of the dominant pearl-colored colonies.  Moderate growth of bacteria was observed in root, stem and leaf bits sterilized up to 35 minutes. Even on surface sterilized roots which were homogenized and inoculated on growth media, dense growth of bacteria was observed there by establishing the presence of bacteria inside the root system. For the first time the presence of growth promoting bacteria on the rhizosphere and endorhizosphere of brinjal (Solanum Melongena L.) cultivars was established.

scholarly journals Profiling of Plant Growth-Promoting Metabolites by Phosphate-Solubilizing Bacteria in Maize Rhizosphere

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1071
Author(s):  
Minchong Shen ◽  
Jiangang Li ◽  
Yuanhua Dong ◽  
Hong Liu ◽  
Junwei Peng ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Control ◽  
Agricultural Practices ◽  
Phosphate Solubilizing Bacteria ◽  
Mean Values ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Microbial Treatments

Microbial treatment has recently been attracting attention as a sustainable agricultural strategy addressing the current problems caused by unreasonable agricultural practices. However, the mechanism through which microbial inoculants promote plant growth is not well understood. In this study, two phosphate-solubilizing bacteria (PSB) were screened, and their growth-promoting abilities were explored. At day 7 (D7), the lengths of the root and sprout with three microbial treatments, M16, M44, and the combination of M16 and M44 (Com), were significantly greater than those with the non-microbial control, with mean values of 9.08 and 4.73, 7.15 and 4.83, and 13.98 and 5.68 cm, respectively. At day 14 (D14), M16, M44, and Com significantly increased not only the length of the root and sprout but also the underground and aboveground biomass. Differential metabolites were identified, and various amino acids, amino acid derivatives, and other plant growth-regulating molecules were significantly enhanced by the three microbial treatments. The profiling of key metabolites associated with plant growth in different microbial treatments showed consistent results with their performances in the germination experiment, which revealed the metabolic mechanism of plant growth-promoting processes mediated by screened PSB. This study provides a theoretical basis for the application of PSB in sustainable agriculture.

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

scholarly journals A Bacterium Isolated From Soil in a Karst Rocky Desertification Region Has Efficient Phosphate-Solubilizing and Plant Growth-Promoting Ability

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinge Xie ◽  
Zongqiang Yan ◽  
Guifen Wang ◽  
Wenzhi Xue ◽  
Cong Li ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gluconic Acid ◽  
Lateral Roots ◽  
Utilization Efficiency ◽  
Phosphate Solubilizing Bacteria ◽  
Karst Rocky Desertification ◽  
Rocky Desertification ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Phosphorus in the soil accessible to plants can easily be combined with calcium ion, the content of which is high in karst rocky desertification (KRD) regions, thereby resulting in a low utilization efficiency of phosphorus. The application of phosphate-solubilizing bacteria (PSB) from the KRD region would facilitate enhanced phosphate availability in the soil. In the present study, the strains belonging to Acinetobacter, Paraburkholderia, and Pseudomonas with efficient phosphate-solubilizing ability were isolated from fruit tree rhizosphere soils in KRD regions. Particularly, Acinetobacter sp. Ac-14 had a sustained and stable phosphate-solubilizing ability (439–448 mg/L, 48–120 h). Calcium carbonate decreased the phosphate-solubilizing ability in liquid medium; however, it did not affect the solubilization index in agar-solidified medium. When cocultivated with Arabidopsis thaliana seedling, Ac-14 increased the number of lateral roots, fresh weight, and chlorophyll content of the seedlings. Metabolomics analysis revealed that Ac-14 could produce 23 types of organic acids, majorly including gluconic acid and D-(-)-quinic acid. Expression of Ac-14 glucose dehydrogenase gene (gcd) conferred Pseudomonas sp. Ps-12 with a sustained and stable phosphate-solubilizing ability, suggesting that the production of gluconic acid is an important mechanism that confers phosphate solubilization in bacteria. Moreover, Ac-14 could also produce indole acetic acid and ammonia. Collectively, the isolated Ac-14 from KRD regions possess an efficient phosphate-solubilizing ability and plant growth-promoting effect which could be exploited for enhancing phosphorus availability in KRD regions. This study holds significance for the improvement of soil fertility and agricultural sustainable development in phosphorus-deficient KRD regions.

scholarly journals Plant-Growth-Promoting Bacteria Mitigating Soil Salinity Stress in Plants

2020 ◽  
Vol 10 (20) ◽  
pp. 7326
Author(s):  
Stefan Shilev
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Soil Salinity ◽  
Soil Salinization ◽  
Plant Growth Promoting Bacteria ◽  
Plant Tolerance ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil deterioration has led to problems with the nutrition of the world’s population. As one of the most serious stressors, soil salinization has a negative effect on the quantity and quality of agricultural production, drawing attention to the need for environmentally friendly technologies to overcome the adverse effects. The use of plant-growth-promoting bacteria (PGPB) can be a key factor in reducing salinity stress in plants as they are already introduced in practice. Plants having halotolerant PGPB in their root surroundings improve in diverse morphological, physiological, and biochemical aspects due to their multiple plant-growth-promoting traits. These beneficial effects are related to the excretion of bacterial phytohormones and modulation of their expression, improvement of the availability of soil nutrients, and the release of organic compounds that modify plant rhizosphere and function as signaling molecules, thus contributing to the plant’s salinity tolerance. This review aims to elucidate mechanisms by which PGPB are able to increase plant tolerance under soil salinity.

Production of Plant Growth Promoting Substances by Phosphate Solubilizing Bacteria Isolated from Vertisols

2007 ◽  
Vol 2 (3) ◽  
pp. 326-333 ◽  
Author(s):  
A. Vikram ◽  
H. Hamzehzarghani . ◽  
A.R. Alagawadi . ◽  
P.U. Krishnaraj . ◽  
B.S. Chandrashekar .
Keyword(s):  
Plant Growth ◽  
Phosphate Solubilizing Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

scholarly journals Plant Growth Stimulation by Microbial Consortia

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 219
Author(s):  
Gustavo Santoyo ◽  
Paulina Guzmán-Guzmán ◽  
Fannie Isela Parra-Cota ◽  
Sergio de los Santos-Villalobos ◽  
Ma. del Carmen Orozco-Mosqueda ◽  
...  
Keyword(s):  
Plant Growth ◽  
Abiotic Stress Tolerance ◽  
Growth Stimulation ◽  
Microbial Consortia ◽  
Plant Growth Promoting Bacteria ◽  
Biotic And Abiotic Stress ◽  
Beneficial Effects ◽  
Plant Growth Stimulation ◽  
Plant Growth Promoting ◽  
Multiple Species

Plant-associated microorganisms play an important role in agricultural production. Although various studies have shown that single microorganisms can exert beneficial effects on plants, it is increasingly evident that when a microbial consortium—two or more interacting microorganisms—is involved, additive or synergistic results can be expected. This occurs, in part, due to the fact that multiple species can perform a variety of tasks in an ecosystem like the rhizosphere. Therefore, the beneficial mechanisms of plant growth stimulation (i.e., enhanced nutrient availability, phytohormone modulation, biocontrol, biotic and abiotic stress tolerance) exerted by different microbial players within the rhizosphere, such as plant-growth-promoting bacteria (PGPB) and fungi (such as Trichoderma and Mycorrhizae), are reviewed. In addition, their interaction and beneficial activity are highlighted when they act as part of a consortium, mainly as mixtures of different species of PGPB, PGPB–Mycorrhizae, and PGPB–Trichoderma, under normal and diverse stress conditions. Finally, we propose the expansion of the use of different microbial consortia, as well as an increase in research on different mixtures of microorganisms that facilitate the best and most consistent results in the field.

Sign in / Sign up

Export Citation Format

Share Document