Determination of major anthocyanin pigments and flavonols in red grape skin of some table grape varieties (Vitis vinifera sp.) by high-performance liquid chromatography–photodiode array detection (HPLC-DAD)

Aim: The aim of this study was the investigation of the anthocyanin and flavonol content in grape skin extracts. Five prevalent anthocyanin-types and four flavonol-types were determined in the skin of three red table grape varieties widely cultivated in El-Tarf (Algeria).Methods and results: The identification of the compounds was performed by HPLC-DAD based on C-18 reversed phase column separation. Results from HPLC analysis showed that malvidin and petunidin-3-O-glucoside were the major anthocyanin glucoside, whereas quercetin-3-O-glucoside was the major flavonol among the four identified.Conclusion: The content of anthocyanins and flavonols in the grape skin of three grapevine (Vitis vinifera) varieties exhibits notable differences among the cultivars studied, confirming their importance in the varietal characterization. The highest concentrations of total anthocyanins and flavonols corresponded to the Gros noir variety. The results of the present study also indicate that the grape skin extracts of these Algerian cultivars can be used as easily accessible source of natural antioxidants. Significance and impact of the study: To the best of our knowledge, this is the first report on the identification of different anthocyanins and flavonols in berry skin from some red grape varieties largely cultivated in this region of Algeria.

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Anthocyanins profile, total phenolics and antioxidant activity of two Romanian red grape varieties: Feteascǎ neagrǎ and Bǎbeascǎ neagrǎ (Vitis vinifera)

Chemical Papers ◽

10.1515/chempap-2015-0163 ◽

2015 ◽

Vol 69 (12) ◽

Cited By ~ 1

Author(s):

Oana Emilia Constantin ◽

Mihaela Skrt ◽

Natasa Poklar Ulrih ◽

Gabriela Râpeanu

Keyword(s):

Antioxidant Activity ◽

Vitis Vinifera ◽

High Performance ◽

Total Phenolics ◽

High Performance Liquid Chromatographic ◽

Phenolic Composition ◽

Total Anthocyanins ◽

Red Grape ◽

Grape Varieties ◽

Liquid Chromatographic

AbstractThe phenolic composition of Fetească neagră and Băbească neagră grapes from Dealul Bujorului vineyard (south-east Romania) was studied using the spectrophotometric and high-performance liquid chromatographic (HPLC) methods. The results revealed significant differences between these cultivars. Total anthocyanins ranged from 0.22-5.98 mg g

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Characterization of Sparkling Wines According to Polyphenolic Profiles Obtained by HPLC-UV/Vis and Principal Component Analysis

Foods ◽

10.3390/foods8010022 ◽

2019 ◽

Vol 8 (1) ◽

pp. 22 ◽

Cited By ~ 7

Author(s):

Anaïs Izquierdo-Llopart ◽

Javier Saurina

Keyword(s):

Principal Component Analysis ◽

High Performance ◽

Principal Component ◽

Component Analysis ◽

Reversed Phase ◽

Sparkling Wine ◽

Grape Skin ◽

Acid Aqueous Solution ◽

Grape Varieties

Cava is a sparkling wine obtained by a secondary fermentation in its own bottle. Grape skin contains several compounds, such as polyphenols, which act like natural protectors and provide flavor and color to the wines. In this paper, a previously optimized method based on reversed phase high performance liquid chromatography (HPLC) with ultraviolet/visible (UV/Vis) detection has been applied to determine polyphenols in cava wines. Compounds have been separated in a C18 core-shell column using 0.1% formic acid aqueous solution and methanol as the components of the mobile phase. Chromatograms have been recorded at 280, 310 and 370 nm to gain information on the composition of benzoic acids, hidroxycinnamic acids and flavonoids, respectively. HPLC-UV/vis data consisting of compositional profiles of relevant analytes has been exploited to characterize cava wines produced from different base wine blends using chemometrics. Other oenological variables, such as vintage, aging or malolatic fermentation, have been fixed over all the samples to avoid their influence on the description. Principal component analysis and other statistic methods have been used to extract of the underlying information, providing an excellent discrimination of samples according to grape varieties and coupages.

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Polyphenolic Characterization of Merlot, Tannat and Syrah Skin Extracts at Different Degrees of Maturity and Anti-Inflammatory Potential in RAW 264.7 Cells

Foods ◽

10.3390/foods10030541 ◽

2021 ◽

Vol 10 (3) ◽

pp. 541

Author(s):

Nawel Benbouguerra ◽

Josep Valls-Fonayet ◽

Stephanie Krisa ◽

François Garcia ◽

Cédric Saucier ◽

...

Keyword(s):

Vitis Vinifera ◽

Ultra Performance Liquid Chromatography ◽

Raw 264.7 Cells ◽

Triple Quadrupole Mass Spectrometer ◽

Polyphenolic Composition ◽

Anti Inflammatory ◽

Red Grape ◽

Species Production ◽

Grape Varieties

(1) Background: Both sensory quality and healthy attributes of Vitis vinifera grapes used for winemaking are closely related with the polyphenolic composition of their skins. (2) Methods: In this study, the polyphenolic characterization (flavan-3-ols, procyanidins, flavonols, stilbenes, anthocyanins) was investigated by ultra performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UPLC-QqQ-MS). Skins from Vitis vinifera Merlot, Tannat, and Syrah red grape varieties cultivated in the south of France at different stages of ripening in 2018 were used. The anti-inflammatory and the antioxidant potential of the extracts were evaluated by the measure of nitric oxide (NO) and the intracellular reactive oxygen species production (ROS) in lipopolysaccharide (LPS)-stimulated macrophages. (3) Results: 41 polyphenols were quantified in all samples. Generally, the flavan-3-ol and procyanidin content decreased during ripening whereas the anthocyanins and stilbenes increased. In addition, as a novelty of this work, a wide identification and characterization of monomeric and oligomeric stilbenes was assessed by using authentic standards isolated in our laboratory, some of them (parthenocissin A and miyabenol C) reported for the first time in Merlot, Tannat and Syrah cultivars. The before-veraison skin extracts of all studied varieties, exhibited higher NO and ROS productions inhibition (>50%) proving both antioxidant and anti-inflammatory properties.

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Flavanol Glycoside Content of Grape Seeds and Skins of Vitis vinifera Varieties Grown in Castilla-La Mancha, Spain

Molecules ◽

10.3390/molecules24214001 ◽

2019 ◽

Vol 24 (21) ◽

pp. 4001 ◽

Cited By ~ 3

Author(s):

José Pérez-Navarro ◽

Guillaume Cazals ◽

Christine Enjalbal ◽

Pedro Miguel Izquierdo-Cañas ◽

Sergio Gómez-Alonso ◽

...

Keyword(s):

Vitis Vinifera ◽

High Performance ◽

Grape Seed ◽

Grape Seeds ◽

Grape Berries ◽

La Mancha ◽

Liquid Chromatography Tandem Mass ◽

Grape Varieties ◽

Grape Seed Extracts ◽

Seed Extracts

Glycosylated flavanols (monoglycosides and diglycosides) in skin and seed extracts of Vitis vinifera grapes grown in Castilla-La Mancha (Spain) were investigated using ultra-high-performance liquid chromatography—tandem mass spectrometry (UHPLC-ESI-QQQ-MS/MS). Six grape varieties (Airén, Tempranillo, the recently identified Albillo Dorado, Montonera del Casar, Moribel, and Tinto Fragoso) were studied over two consecutive years (2016 and 2017). A total of twenty monomeric flavanol monoglycosides, four diglycosylated monomers, and three dimeric flavanol monoglycosides were detected in all grape samples. The diversity observed in the composition of glycosylated flavanol in the grape berries suggests a strong influence of variety and grape tissue (skin or seed). Monomeric flavanol glycosides were more abundant in grape seed extracts, in contrast with monoglycosylated dimeric forms. In addition, the glycosylated flavanol content was related to berry color in grape skins, with higher concentrations measured in black grape varieties.

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Development and Validation of Reversed-Phase Column High-Performance Liquid Chromatographic and First-Derivative UV Spectrophotometric Methods for Estimation of Voriconazole in Oral Suspension Powder

Journal of AOAC International ◽

10.1093/jaoac/91.5.1070 ◽

2008 ◽

Vol 91 (5) ◽

pp. 1070-1074 ◽

Cited By ~ 4

Author(s):

Arun M Prajapati ◽

Satish A Patel ◽

Natvarlal J Patel ◽

Dipti B Patel ◽

Sejal K Patel

Keyword(s):

High Performance ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Hplc Method ◽

Oral Suspension ◽

Photodiode Array Detection ◽

Spectrophotometric Methods ◽

First Derivative ◽

Rp Hplc ◽

Liquid Chromatographic

Abstract This research paper describes validated reversed-phase high-performance column liquid chromatographic (RP-HPLC) and first-derivative UV spectrophotometric methods for the estimation of voriconazole (VOR) in oral suspension powder. The RP-HPLC separation was achieved on Phenomenex C18 column (250 4.6 mm id, 5 m particle size) using wateracetonitrile (40 + 60, v/v; pH adjusted to 4.5 0.02 with acetic acid) as the mobile phase at a flow rate of 1.4 mL/min and ambient temperature. Quantification was achieved with photodiode array detection at 255 nm over the concentration range of 0.11 g/mL with mean recovery of 99.49 0.83 for VOR by the RP-HPLC method. Quantification was achieved with UV detection at 266 nm over the concentration range of 820 g/mL with mean recovery of 99.74 0.664 for VOR by the first-derivative UV spectrophotometric method. These methods are simple, precise, and sensitive, and they are applicable for the determination of VOR in oral suspension powder.

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Sustainable Micro-Scale Extraction of Bioactive Phenolic Compounds from Vitis vinifera Leaves with Ionic Liquid-Based Surfactants

Molecules ◽

10.3390/molecules25133072 ◽

2020 ◽

Vol 25 (13) ◽

pp. 3072 ◽

Cited By ~ 1

Author(s):

Giulia Mastellone ◽

Idaira Pacheco-Fernández ◽

Patrizia Rubiolo ◽

Verónica Pino ◽

Cecilia Cagliero

Keyword(s):

Ionic Liquid ◽

Phenolic Compounds ◽

Vitis Vinifera ◽

High Performance ◽

Photodiode Array Detection ◽

Micro Scale ◽

Simple Strategy ◽

Analytical System ◽

Solid Liquid ◽

Extraction Phase

This paper proposes a new sustainable and simple strategy for the micro-scale extraction of phenolic compounds from grapevine leaves with analytical purpose. The method is based on a microwave-assisted solid-liquid extraction approach (MA-SLE), using an aqueous solution of an ionic liquid (IL)-based surfactant as extraction phase. The method does not require organic solvents, nor any clean-up step, apart from filtration prior to the injection in the analytical system. Two IL-based surfactants were evaluated, and the method was optimized by using experimental designs, resulting in the use of small amounts of sample (100 mg) and extraction phase (2.25 mL), low concentrations of the selected 1-hexadecyl-3-butyl imidazolium bromide IL (0.1 mM), and 30 min of extraction time. The proposed methodology was applied for the determination of the polyphenolic pattern of six different varieties of Vitis vinifera leaves from the Canary Islands, using high-performance liquid chromatography and photodiode array detection for the quantification of the compounds. The proposed MA-SLE approach was greener, simpler, and more effective than other methods, while the results from the analysis of the leaves samples demonstrate that these by-products can be exploited as a source of natural compounds for many applications.

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