scholarly journals The Effect of Rhizome Extract of Curcuma (Curcuma xanthorriza Roxb) for Cell Injury in Histopathology of Liver Tissue of Male White Mice (Mus musculus L.) Strain BALB/C Infected by Plasmodium berghei Anka

2019 ◽  
Vol 12 (03) ◽  
pp. 01-07
Author(s):  
Bagah Restu Pambudi ◽  
Prawesty Diah Utami ◽  
Retno Budiarti
Keyword(s):  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Liver Tissue ◽  
Cell Injury ◽  
Plasmodium Berghei Anka

Pengaruh Pemberian Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) Terhadap Jumlah Eritrosit Mencit (Mus musculus L.) Jantan BALB/c yang Diinokulasi Plasmodium Berghei Anka

2018 ◽  
Vol 15 (2) ◽  
pp. 192
Author(s):  
AZARINE NEIRA AVISHA ◽  
PRAWESTY DIAH UTAMI
Keyword(s):  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Control Group ◽  
Plasmodium Berghei Anka ◽  
Post Test ◽  
Curcuma Xanthorrhiza

<p>Malaria masih menjadi salah satu penyakit infeksi yang paling penting di negara tropis karena dapat menyebabkan anemia berat dan kematian. Dewasa ini, penatalaksanaan untuk malaria sendiri semakin terbatas karena adanya resistensi<a href="#_msocom_1">[O11]</a> . Oleh sebab itu, diperlukan adanya penemuan senyawa baru untuk menanggulanginya. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak rimpang temulawak (<em>Curcuma xanthorrhiza</em> Roxb.)  terhadap jumlah eritrosit mencit (<em>Mus musculus</em> L.) jantan BALB/c yang diinokulasi <em>Plasmodium berghei</em> ANKA. Penelitian dilakukan secara eksperimental dengan desain <em>post-test only control group</em> dengan menggunakan lima kelompok mencit. Satu kelompok mencit dibiarkan normal sedangkan empat kelompok lain diinokulasi <em>Plasmodium berghei </em>ANKA, dimana satu kelompok diberi aquades dan tiga kelompok diterapi ekstrak temulawak  (<em>Curcuma xanthorrhiza</em> Roxb.) dengan dosis 150 mg/KgBB, 100 mg/KgBB, dan 50 mg/KgBB selama empat hari. Pada hari kelima dilakukan pemeriksaan darah untuk mengetahui jumlah eritrosit. Hasil penelitian ini menunjukkan bahwa rerata jumlah eritrosit mencit (<em>Mus musculus</em> L.) jantan BALB/c yang diinokulasi <em>Plasmodium berghei</em> ANKA dandiberi ekstrak rimpang temulawak  (<em>Curcuma xanthorrhiza</em> Roxb.)dengan dosis 150 mg/KgBB dan 50 mg/KgBB menurun secara tidak bermakna. Sedangkan rerata jumlah eritrosit mencit (<em>Mus musculus</em> L.) jantan BALB/c yang diinokulasi <em>Plasmodium berghei</em> ANKA dan diberi ekstrak rimpang temulawak (<em>Curcuma xanthorrhiza</em> Roxb.)  dengan dosis 100 mg/KgBB menurun bermakna (p=0,004).</p><p> </p><p><strong>Kata Kunci: </strong>Malaria, temulawak (<em>Curcuma xanthorrhiza</em> Roxb.), Eritrosit, <em>Plasmodium berghei</em> ANKA.</p><div><hr align="left" size="1" width="33%" /><div><div><p> <a href="#_msoanchor_1">[O11]</a>Resistensi terhadap apa?</p></div></div></div>

scholarly journals Effect of Curcuma Xanthorriza Roxb Extract on Hemozoin Pigment Deposition In The Liver Tissue Of BALB /C Mice Infected With Plasmodium berghei ANKA

2020 ◽  
Vol 1 (1) ◽  
pp. 28-33
Author(s):  
Prawesty Utami ◽  
Irmawati Dikwan ◽  
Ali Wibowo
Keyword(s):  
Plasmodium Berghei ◽  
Liver Tissue ◽  
Histopathological Examination ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Plasmodium Berghei Anka ◽  
Treatment Groups ◽  
Hepatocellular Damage ◽  
Significant Difference

Hepatocellular damage is one of the histopathologic features of malaria infection. The presence of large amounts of hemozoin pigment in the liver is associated with hepatocellular damage. Curcuma xanthorriza Roxb / curcumin as an anti-inflammatory and natural antioxidant which has a hepatoprotector function. The purpose of these study was to determine the effect of curcuma rhizome (Curcuma xanthorrhiza Roxb) extract on hemozoin pigment in the liver tissue of BALB/C mice infected with Plasmodium berghei ANKA/ PbA. Method: using 25 groups of BALB/C mice, divided into 5 groups: negative control group; positive control group and 4 treatment groups infected with PbA with extract dose of 150 mg / KgBW, 100 mg / KgBW, and 50 mg / KgBW for four days. On the fifth day the observation of liver tissue to determine deposition of hemozoin pigments by histopathological examination. Results: Statistical analysis showed that descriptively there was a decrease in hemozoin pigment in the liver tissue of mice (150 mg / KgBW extract dose decreased the most hemozoin pigment compared to other doses), but there was no statistically significant difference between the treatment groups with positive controls (p> 0.05). Conclusions: The administration of curcuma extract (Curcuma xanthorriza Roxb) has the potential to reduce the deposition of hemozoin pigment in liver tissue in BALB/C mice infected with Plasmodium berghei ANKA although statistically it is not significant.

Ginger Extract Has An Ability To Safe C57BL / 6-WT Mice From Cerebral Malaria Produced By Plasmodium Berghei ANKA

2018 ◽  
pp. 1
Author(s):  
سالم رمضان على السريتى ◽  
نوارة امراجع الازيرق العمارى
Keyword(s):  
Cerebral Malaria ◽  
Plasmodium Berghei ◽  
Plasmodium Berghei Anka ◽  
Ginger Extract

scholarly journals Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile

2020 ◽  
Vol 4 (2) ◽  
pp. 179
Author(s):  
Kartika Arum Wardani ◽  
Kholida Nur Aini ◽  
Heny Arwati ◽  
Willy Sandhika
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Plasmodium Berghei Anka ◽  
Concentration Dependent Manner ◽  
Control Group Design ◽  
Bile Concentration

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of  Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: [email protected]

scholarly journals The alteration of biochemical parameters leading to organ damage during Plasmodium berghei ANKA infection in mice

2017 ◽  
Vol 5 (10) ◽  
pp. 447-450
Author(s):  
Sukanya Pattarapo ◽  
◽  
Kittikarn Kittikarn Ratanavijarn ◽  
Voravuth Somsak ◽  
◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Biochemical Parameters ◽  
Organ Damage ◽  
Plasmodium Berghei Anka

scholarly journals Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Okky Irtanto ◽  
Alex Pangkahila ◽  
IGM Aman
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Mus Musculus ◽  
Liver Tissue ◽  
Day Treatment ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstract: Overtraining accelerates aging due to the excessive production of free radicals that can cause oxidative stress. Banana floret extract contains bioactive compounds with antioxidant capacity which can increase the body's defence to deal with the oxidative stress by increasing the level of superoxide dismutase (SOD). This study was aimed to prove that the banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in overtraining-induced mice (Mus musculus) BALB/c liver. This was a true experimental study with the post-test only control group design. Subjects were 36 male mice (Mus musculus), BALB/c strain, 12 weeks old, weighing 20-22 g, which were divided into two groups with 18 mice each. The control group (P0) was treated with a placebo of 1 ml aquadest and overtrained for 14 days meanwhile the treatment group (P1) was treated with banana (Musa x paradisiaca) floret extract of 400 mg/kgBW/day and overtrained for 14 days. The results showed that after 14-day treatment, the mean SOD level in the liver tissue of the P0 group was 568.82±9.558 U/mg protein whereas in the P1 group was 588.37±10.629 U/mg protein (P < 0.01). The t-independent test showed a t value of -5.804 and a P value of 0.000 which indicated that after treatment, the levels of SOD in liver tissue of both groups were significantly different. Conclusion: Banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in the liver tissue of overtraining-induced mice (Mus musculus) BALB/c.Keywords: banana floret, SOD, liver, overtrainingAbstrak: Aktivitas fisik berlebih mempercepat penuaan karena meningkatkan produksi radikal bebas yang dapat menyebabkan stres oksidatif. Ekstrak floret pisang mengandung senyawa bioaktif dengan kapasitas antioksidan yang dapat meningkatkan pertahanan tubuh dalam menghadapi stres oksidatif melalui peningkatan kadar superoksida dismutase (SOD). Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 36 ekor mencit (Mus Musculus) BALB/c, jantan, berumur 12 minggu, berat badan 20-22 gr, yang dibagi menjadi dua kelompok masing-masing berjumlah 18 ekor mencit. Kelompok kontrol (P0) diberikan plasebo berupa aquadest sebanyak 1 ml dengan aktivitas fisik berlebih selama 14 hari, dan kelompok perlakuan (P1) diberikan ekstrak floret pisang raja (Musa x paradisiaca) dosis 400 mg/kgBB mencit per hari dicampur aquadest hingga 1 ml dengan aktivitas fisik berlebih selama 14 hari. Hasil penelitian menunjukkan rerata kadar SOD jaringan hati pada kelompok kontrol (P0) sesudah perlakuan (post-test) ialah 568,82±9,558 U/mg protein, sedangkan pada kelompok perlakuan (P1) ialah 588,37± 10,629 U/mg protein. Analisis kemaknaan dengan T-Independent mendapatkan nilai t= -5,804 dan nilai P = 0,000 yang menunjukkan bahwa sesudah perlakuan (post-test), kadar SOD jaringan hati pada kedua kelompok berbeda sangat bermakna. Simpulan: Ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih.Kata kunci: floret pisang raja, SOD, hati, aktivitas fisik berlebih

scholarly journals Histology of Mice (Mus Musculus) Liver Tissue Fixed with Carnoy's Solution With Variation of 4 Hours, 8 Hours and 12 Hours

2021 ◽  
Vol 3 (1) ◽  
pp. 38-43
Author(s):  
Arin Dwi Afrida ◽  
Djoko Priyatno
Keyword(s):  
Mus Musculus ◽  
Liver Tissue ◽  
Histopathological Examination ◽  
Observational Research ◽  
Cell Nuclei ◽  
Pathology Laboratory ◽  
Descriptive Research ◽  
Carnoy’S Solution ◽  
Time Variations ◽  
Examination Process

Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.

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