Pengaruh Pemberian Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) Terhadap Jumlah Eritrosit Mencit (Mus musculus L.) Jantan BALB/c yang Diinokulasi Plasmodium Berghei Anka

2018 ◽  
Vol 15 (2) ◽  
pp. 192
Author(s):  
AZARINE NEIRA AVISHA ◽  
PRAWESTY DIAH UTAMI
Keyword(s):  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Control Group ◽  
Plasmodium Berghei Anka ◽  
Post Test ◽  
Curcuma Xanthorrhiza

<p>Malaria masih menjadi salah satu penyakit infeksi yang paling penting di negara tropis karena dapat menyebabkan anemia berat dan kematian. Dewasa ini, penatalaksanaan untuk malaria sendiri semakin terbatas karena adanya resistensi<a href="#_msocom_1">[O11]</a> . Oleh sebab itu, diperlukan adanya penemuan senyawa baru untuk menanggulanginya. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak rimpang temulawak (<em>Curcuma xanthorrhiza</em> Roxb.)  terhadap jumlah eritrosit mencit (<em>Mus musculus</em> L.) jantan BALB/c yang diinokulasi <em>Plasmodium berghei</em> ANKA. Penelitian dilakukan secara eksperimental dengan desain <em>post-test only control group</em> dengan menggunakan lima kelompok mencit. Satu kelompok mencit dibiarkan normal sedangkan empat kelompok lain diinokulasi <em>Plasmodium berghei </em>ANKA, dimana satu kelompok diberi aquades dan tiga kelompok diterapi ekstrak temulawak  (<em>Curcuma xanthorrhiza</em> Roxb.) dengan dosis 150 mg/KgBB, 100 mg/KgBB, dan 50 mg/KgBB selama empat hari. Pada hari kelima dilakukan pemeriksaan darah untuk mengetahui jumlah eritrosit. Hasil penelitian ini menunjukkan bahwa rerata jumlah eritrosit mencit (<em>Mus musculus</em> L.) jantan BALB/c yang diinokulasi <em>Plasmodium berghei</em> ANKA dandiberi ekstrak rimpang temulawak  (<em>Curcuma xanthorrhiza</em> Roxb.)dengan dosis 150 mg/KgBB dan 50 mg/KgBB menurun secara tidak bermakna. Sedangkan rerata jumlah eritrosit mencit (<em>Mus musculus</em> L.) jantan BALB/c yang diinokulasi <em>Plasmodium berghei</em> ANKA dan diberi ekstrak rimpang temulawak (<em>Curcuma xanthorrhiza</em> Roxb.)  dengan dosis 100 mg/KgBB menurun bermakna (p=0,004).</p><p> </p><p><strong>Kata Kunci: </strong>Malaria, temulawak (<em>Curcuma xanthorrhiza</em> Roxb.), Eritrosit, <em>Plasmodium berghei</em> ANKA.</p><div><hr align="left" size="1" width="33%" /><div><div><p> <a href="#_msoanchor_1">[O11]</a>Resistensi terhadap apa?</p></div></div></div>

scholarly journals Pengaruh Pemberian Ekstrak Temulawak (Curcuma Xanthorrizha Roxb.) Terhadap Level Nekrosis pada Jaringan Lien Mencit Putih (Mus Musculus L.) Jantan Galur Balb/C yang Diinokulasi Plasmodium berghei anka

2019 ◽  
Vol 16 (2) ◽  
pp. 186
Author(s):  
MUHAMMAD REYHAN ARSYA ◽  
PRAWESTY DIAH UTAMI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Histopathological Examination ◽  
Positive Control ◽  
Control Group ◽  
Control Group Design ◽  
Mortality And Morbidity ◽  
Treatment Groups ◽  
Post Test ◽  
Curcuma Xanthorrhiza

<p><strong>Abstract</strong></p><p><strong>Background : </strong>Malaria is a disease caused by the <em>Plasmodium</em> parasite and is transmitted by the <em>Anopheles</em> mosquito and is still a health problem in Indonesia due to high mortality and morbidity. One form of a severe complication of malaria in addition to cerebral malaria is a function failure of the spleen. Today, the management of malaria is increasingly limited due to resistance. Therefore, further development is needed to find new innovations in malaria treatment.</p><p><strong>Purpose : </strong>The purpose of this study was to determine the effect of temulawak rhizome extract (<em>Curcuma xanthorhizza</em> Roxb.) On the level of necrosis in the spleen tissue of male BALB / c mice (<em>Mus musculus</em> L.) inoculated with <em>Plasmodium berghei</em> ANKA.</p><p><strong>Methods :</strong>Experimental research used a post-test only control group design that used five groups of mice. One group of mice was left normal while the other four groups were inoculated with <em>Plasmodium berghei</em> ANKA, positive control groups were given aquades and three treatment groups treated with temulawak extract (<em>Curcuma xanthorrhiza</em> Roxb.) With a dose of 150 mg / KgBB, 100 mg / KgBB, and 50 mg / KgBB for four day. On the fifth day an observation of the level of necrosis in the spleen organ of mice to determine the level of necrosis by histopathological examination using a light microscope.</p><p><strong>Conclusion and Result : </strong>The results of this study indicate that the administration of ginger rhizome extract (<em>Curcuma xanthorriza</em> Roxb.) Has an influence on the level of necrosis of male mice (<em>Mus musculus</em> L.) BALB / c inoculated with <em>Plasmodium berghei</em> ANKA α = 0,002 (ρ&lt;0,05), where the administration of temulawak extract can increase necrosis levels compared to the control group . This is probably due to the lack of temulawak extract dosage and lack of observation in this study.</p><p> </p><p><strong>Keywords </strong>: Malaria, curcuma (<em>Curcuma xanthorrhiza</em> Roxb.), Necrosis level, <em>Plasmodium berghei</em> ANKA</p>

scholarly journals Infeksi Plasmodium Berghei Anka pada Mencit Galur Swiss Webster dan C57bl/6 Sebagai Model Malaria Serebral

2020 ◽  
Vol 48 (1) ◽  
Author(s):  
Putri Reno Intan ◽  
Tati Febrianti ◽  
Rita Marleta Dewi
Keyword(s):  
Plasmodium Falciparum ◽  
Cerebral Malaria ◽  
Experimental Animal ◽  
Plasmodium Berghei ◽  
Control Group ◽  
Plasmodium Berghei Anka ◽  
Animal Testing ◽  
Control Group Design ◽  
Post Test ◽  
The Brain

Abstract Cerebral malaria is a complication of Plasmodium falciparum infection and can cause death in humans. Plasmodium berghei ANKA (PbA) infection in C57BL/6 strain is widely used for cerebral malaria research. However, research to assess differences in brain histopathology, TNFα levels and the degree of parasitemia in Swiss websters infected with PbA are still limited. Therefore an infection with P. berghei ANKA strain was carried out on Swiss webster mice and C57BL/6 as a model of cerebral malaria. This research is a laboratory experiment with a post-test only control group design. Each experimental animal was divided into 3 groups namely PbA group (infected and given aquades), DHP (infected and given Dihidroartemisinin piperakuin), and healthy (mice not infected with PbA called aquades). Animal testing tried to examine parasitemia by microscopic counts on thin blood smear, calculation of serum TNFα levels by ELISA method and histopathology of the brain and slide microscopic examination with Haematoxylin Eosin staining conducted at the Experimental Animal Laboratory, Parasite Laboratory, Puslitbang BTDK, NIHRD; and Balitvet Pathology Laboratory by the research team. The results showed a peak percentage of parasitemia in the PbA group on day 4 for the Swiss webster strain (68.8%) and on the 5th day for the C57BL/6 strain (43.7%). The percentage of parasitemia was higher in the Swiss webster strain than in the C57BL/6 strain. TNFα levels in the Swiss webster strain (3.6 pg/ml) were higher than TNFα levels in the C57BL/6 strain (0.18 pg/ml). Histopathological changes in the brain consisting of lymphocyte cells, infiltration, macrophages, gliosis, necrosis, vacuolization and malaria parasitemia were found in both strains. The results showed that Swiss webster mice can be used as a model of cerebral malaria when seen from the clinical picture, the percentage of parasitemia, serum TNFα levels, macroscopic and microscopic brains that have similarities to the C57BL/6 strain. Keywords: Cerebral malaria, Plasmodium berghei ANKA, Swiss webster, C57BL/6. Abstrak Malaria serebral merupakan salah satu komplikasi infeksi Plasmodium falciparum dan dapat menyebabkan kematian pada manusia. Infeksi Plasmodium berghei ANKA (PbA) pada mencit galur C57BL/6 banyak digunakan untuk penelitian malaria serebral. Di Indonesia, mencit Swiss webster banyak digunakan sebagai hewan coba untuk malaria, namun penelitian untuk menilai perbedaaan histopatologi otak, kadar TNFα dan derajat parasitemia pada Swiss webster yang diinfeksi PbA masih terbatas. Oleh sebab itu dilakukan infeksi P. berghei galur ANKA pada mencit Swiss webster dan C57BL/6 sebagai model malaria serebral. Penelitian ini merupakan eksperimen laboratorium dengan desain post-test only control group. Masing-masing galur hewan coba dibagi menjadi 3 kelompok yaitu kelompok PbA (diinfeksi dan diberi akuades), DHP (diinfeksi dan diberi Dihidroartemisinin piperakuin), dan sehat (mencit yang tidak diinfeksi PbA yang diberi akuades). Pengujian pada hewan coba meliputi pemeriksaan parasitemia dengan penghitungan kepadatan parasit secara mikroskopis pada ulas darah tipis, penghitungan kadar TNFα serum dengan metode ELISA serta gambaran histopatologi sediaan otak dengan pewarnaan Haematoksilin Eosin dilakukan di Laboratorium Hewan Coba, Laboratorium Parasit Puslitbang BTDK, Badan Litbangkes; dan Laboratorium Patologi Balitvet oleh tim peneliti. Hasil penelitian menunjukkan puncak persentase parasitemia kelompok PbA pada hari ke- 4 untuk galur Swiss webster (68.8%) sementara untuk galur C57BL/6 (43.7%) pada hari ke-5. Hal ini berarti bahwa puncak parasitemia lebih cepat dan tinggi terjadi pada galur Swiss webster dibandingkan pada galur C57BL/6. Demikian juga untuk kadar TNFα didapatkan bahwa pada galur Swiss webster (3.6 pg/ml) lebih tinggi dibandingkan galur C57BL/6 (0.18 pg/ml). Perubahan histopatologik otak berupa infiltrasi sel limfosit, makrofag, gliosis, nekrosis, vakuolisasi dan parasitemia malaria ditemukan pada kedua galur. Hasil penelitian menunjukkan bahwa mencit galur Swiss webster dapat dijadikan sebagai model malaria serebral. Kata kunci: Malaria serebral, Plasmodium berghei ANKA, Swiss webster, C57BL/6.

scholarly journals Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile

2020 ◽  
Vol 4 (2) ◽  
pp. 179
Author(s):  
Kartika Arum Wardani ◽  
Kholida Nur Aini ◽  
Heny Arwati ◽  
Willy Sandhika
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Plasmodium Berghei Anka ◽  
Concentration Dependent Manner ◽  
Control Group Design ◽  
Bile Concentration

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of  Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: [email protected]

scholarly journals Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Okky Irtanto ◽  
Alex Pangkahila ◽  
IGM Aman
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Mus Musculus ◽  
Liver Tissue ◽  
Day Treatment ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstract: Overtraining accelerates aging due to the excessive production of free radicals that can cause oxidative stress. Banana floret extract contains bioactive compounds with antioxidant capacity which can increase the body's defence to deal with the oxidative stress by increasing the level of superoxide dismutase (SOD). This study was aimed to prove that the banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in overtraining-induced mice (Mus musculus) BALB/c liver. This was a true experimental study with the post-test only control group design. Subjects were 36 male mice (Mus musculus), BALB/c strain, 12 weeks old, weighing 20-22 g, which were divided into two groups with 18 mice each. The control group (P0) was treated with a placebo of 1 ml aquadest and overtrained for 14 days meanwhile the treatment group (P1) was treated with banana (Musa x paradisiaca) floret extract of 400 mg/kgBW/day and overtrained for 14 days. The results showed that after 14-day treatment, the mean SOD level in the liver tissue of the P0 group was 568.82±9.558 U/mg protein whereas in the P1 group was 588.37±10.629 U/mg protein (P < 0.01). The t-independent test showed a t value of -5.804 and a P value of 0.000 which indicated that after treatment, the levels of SOD in liver tissue of both groups were significantly different. Conclusion: Banana (Musa x paradisiaca) floret extract could prevent the decrease of superoxide dismutase (SOD) levels in the liver tissue of overtraining-induced mice (Mus musculus) BALB/c.Keywords: banana floret, SOD, liver, overtrainingAbstrak: Aktivitas fisik berlebih mempercepat penuaan karena meningkatkan produksi radikal bebas yang dapat menyebabkan stres oksidatif. Ekstrak floret pisang mengandung senyawa bioaktif dengan kapasitas antioksidan yang dapat meningkatkan pertahanan tubuh dalam menghadapi stres oksidatif melalui peningkatan kadar superoksida dismutase (SOD). Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 36 ekor mencit (Mus Musculus) BALB/c, jantan, berumur 12 minggu, berat badan 20-22 gr, yang dibagi menjadi dua kelompok masing-masing berjumlah 18 ekor mencit. Kelompok kontrol (P0) diberikan plasebo berupa aquadest sebanyak 1 ml dengan aktivitas fisik berlebih selama 14 hari, dan kelompok perlakuan (P1) diberikan ekstrak floret pisang raja (Musa x paradisiaca) dosis 400 mg/kgBB mencit per hari dicampur aquadest hingga 1 ml dengan aktivitas fisik berlebih selama 14 hari. Hasil penelitian menunjukkan rerata kadar SOD jaringan hati pada kelompok kontrol (P0) sesudah perlakuan (post-test) ialah 568,82±9,558 U/mg protein, sedangkan pada kelompok perlakuan (P1) ialah 588,37± 10,629 U/mg protein. Analisis kemaknaan dengan T-Independent mendapatkan nilai t= -5,804 dan nilai P = 0,000 yang menunjukkan bahwa sesudah perlakuan (post-test), kadar SOD jaringan hati pada kedua kelompok berbeda sangat bermakna. Simpulan: Ekstrak floret pisang raja (Musa x paradisiaca) dapat mencegah penurunan kadar SOD pada hati mencit (Mus musculus) BALB/c dengan aktivitas fisik berlebih.Kata kunci: floret pisang raja, SOD, hati, aktivitas fisik berlebih

scholarly journals Effects of Red Fruit (Pandanus conoideus Lam) Oil on Malondialdehyde Level and Spermatozoa Quality in Mice (Mus musculus) Exposed to Monosodium Glutamate

2018 ◽  
Vol 54 (2) ◽  
pp. 84 ◽  
Author(s):  
Widayati Agustina ◽  
Widjiati Widjiati ◽  
Alfiah Hayati
Keyword(s):  
Mus Musculus ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Distilled Water ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Fruit Oil

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.

scholarly journals Pengaruh Pemberian Sakarin terhadap Aktivitas Alanine Aminotransferase Serum Mencit Diabetes Melitus yang Diinduksi Aloksan

2018 ◽  
Vol 7 (1) ◽  
pp. 94
Author(s):  
Mhicya Utami R ◽  
Cimi Ilmiawati ◽  
Elmatris Sy
Keyword(s):  
Mus Musculus ◽  
Alanine Aminotransferase ◽  
Control Group ◽  
Post Test ◽  
Normal Alt ◽  
Diabetes Melitus

Sakarin dijadikan pemanis alternatif pada penderita Diabetes melitus (DM) karena tidak mempengaruhi kadar glukosa darah. Keamanan sakarin mulai diperdebatkan berdasarkan temuan bahwa sakarin mengakibatkan kerusakan hepar yang ditunjukkan dengan peningkatan aktivitas alanine aminotransferase (ALT) serum. Tujuan penelitian ini adalah melihat pengaruh pemberian sakarin terhadap aktivitas ALT serum mencit diabetes melitus yang diinduksi aloksan. Jenis penelitian ini adalah eksperimental dengan rancangan post-test only control group sejak bulan Agustus sampai Oktober 2015 di Laboratorium Farmakologi Fakultas Farmasi Universitas Andalas. Penelitian dilakukan terhadap 20 mencit (Mus musculus) putih jantan diabetes melitus yang diinduksi aloksan yang dibagi menjadi empat kelompok. Kelompok kontrol diberi akuades dan kelompok perlakuan diberi sakarin dengan dosis masing masing 22,75; 45,5; dan 91 mg/kgBB secara oral selama 28 hari. Nilai aktivitas ALT diukur pada akhir penelitian dengan menggunakan alat chemistry analyzer dengan metode spektrofotometri. Hasil penelitian menunjukkan peningkatan nilai aktivitas ALT pada semua kelompok percobaan dibandingkan dengan nilai normal ALT. Analisis statistik mendapatkan aktivitas ALT yang tidak berbeda bermakna antara kelompok percobaan (p = 0,264). Berdasarkan penelitian dapat disimpulkan bahwa pemberian sakarin selama 28 hari tidak memberikan efek yang bermakna pada peningkatan ALT serum mencit diabetes melitus yang diinduksi aloksan.

scholarly journals Uji Aktivitas Ekstrak Biji Caesalpinia Sp terhadap Jumlah Eritrosit Mus musculus yang Diinfeksi Plasmodium berghei serta Implementasinya dalam Pembelajaran Kimia

2018 ◽  
Vol 2 (2) ◽  
pp. 196-199
Author(s):  
Nurleli Nurleli ◽  
Agus Sundaryono ◽  
Aceng Ruyani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Active Compounds ◽  
Chemistry Learning ◽  
Layer Chromatography

AbstraCT[Activity test of Caesalpinia sp seed extract on erythrocytes amount of Mus musculus infected by Plasmodium berghei and its implementation in chemistry learning]. The aims of this study were to (1) determine the activity of seed extract (Caesalpinia sp) on the number of Erythrocytes of M. Musculus of Swiss Webster Strain infected by P.berghei, (2) Find  the active compounds obtained from the isolation of Caesalpinia sp seed extract (3) Find the differences in student learning outcomes about the separation of secondary metabolites using thin layer chromatography (TLC) which is taught in chemistry learning by using LKS. In this study Caesalpinia sp seed extract was purified by thin layer chromatography and column coromatography, and was identified by IR and 1H-NMR spectroscopy. M. musculus infected with P. berghei was given Caesalpinia sp seed extract at a dose of 0.028 g/Kgbb, 0.056 g/Kgbb, and 0.084 g / Kgbb by gavage, while the positive control group was given Kloroquin diphosphate, negative control was given olive oil. Caesalpinia sp seed extract with a dose of 0.056 g/Kgbb inhibited the highest plasmodium, which was 42.89% and even exceeded the malaria drug commonly used chloroquin diphosphate 29.85%. Caesalpinia sp seed extract capable of reducing plasmodium in erythrocytes is also able to increase the number of uninfected erythrocytes. The active compounds obtained from the isolation of Caesalpinia sp seed extract are alkaloids, flavonoids, saponins, and terpenoids. In the implementation of extracurricular group learning is taught by using worksheets. There are significant differences where the average posttest score is 88.67 and the average pretest is 32.1. Keywords: Caesalpinia Sp.; Erythrocyte; Plasmodium berghei; Mus muscullus; Student working sheet.

scholarly journals Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50

2014 ◽  
Vol 13 (2) ◽  
pp. 86
Author(s):  
Hendrik Setia Budi ◽  
Ira Arundina ◽  
Retno Indrawati ◽  
Leonita Widyana Mahardikasari
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Toxicity Test ◽  
Control Group ◽  
Treatment Groups ◽  
Musa Paradisiaca ◽  
Significant Difference ◽  
Post Test ◽  
Stem Extract ◽  
Banana Stem

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.

scholarly journals Uji Aktivitas Ekstrak Biji Caesalpinia Sp terhadap Jumlah Eritrosit Mus musculus yang Diinfeksi Plasmodium berghei serta Implementasinya dalam Pembelajaran Kimia

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Nurleli Nurleli ◽  
Agus Sundaryono ◽  
Aceng Ruyani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Active Compounds ◽  
Chemistry Learning ◽  
Layer Chromatography

AbstraCT[Activity test of Caesalpinia sp seed extract on erythrocytes amount of Mus musculus infected by Plasmodium berghei and its implementation in chemistry learning]. The aims of this study were to (1) determine the activity of seed extract (Caesalpinia sp) on the number of Erythrocytes of M. Musculus of Swiss Webster Strain infected by P.berghei, (2) Find  the active compounds obtained from the isolation of Caesalpinia sp seed extract (3) Find the differences in student learning outcomes about the separation of secondary metabolites using thin layer chromatography (TLC) which is taught in chemistry learning by using LKS. In this study Caesalpinia sp seed extract was purified by thin layer chromatography and column coromatography, and was identified by IR and 1H-NMR spectroscopy. M. musculus infected with P. berghei was given Caesalpinia sp seed extract at a dose of 0.028 g/Kgbb, 0.056 g/Kgbb, and 0.084 g / Kgbb by gavage, while the positive control group was given Kloroquin diphosphate, negative control was given olive oil. Caesalpinia sp seed extract with a dose of 0.056 g/Kgbb inhibited the highest plasmodium, which was 42.89% and even exceeded the malaria drug commonly used chloroquin diphosphate 29.85%. Caesalpinia sp seed extract capable of reducing plasmodium in erythrocytes is also able to increase the number of uninfected erythrocytes. The active compounds obtained from the isolation of Caesalpinia sp seed extract are alkaloids, flavonoids, saponins, and terpenoids. In the implementation of extracurricular group learning is taught by using worksheets. There are significant differences where the average posttest score is 88.67 and the average pretest is 32.1. Keywords: Caesalpinia Sp.; Erythrocyte; Plasmodium berghei; Mus muscullus; Student working sheet.

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