scholarly journals Assessment of in Vivo Oxidative Stress Biomarkers in Relation to Disease Progression and Cell Proliferation in Benign and Malignant Breast Diseases

2016 ◽  
Author(s):  
Kanchan Karki ◽  
Deepti Pande ◽  
Reena Negi ◽  
Ranjana Khanna ◽  
H D Khanna
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Vitamin E ◽  
Vitamin A ◽  
Vitamin C ◽  
Proliferation Index ◽  
Breast Diseases ◽  
Enzymatic Antioxidants ◽  
Cell Proliferation Activity ◽  
Proliferation Activity

The present study was aimed to evaluate the levels of oxidative stress markers in breast diseases by measuring the 8-hydoxy-2-deoxyguanosine (8-OHdG), vitamin A, vitamin C, vitamin E and total antioxidant status (TAS) alterations in relation to cell proliferation activity and disease progression. Significant increases in the level of oxidative damage marker 8-OHdG and cell proliferation activity were observed in breast carcinoma patients in comparison to benign and normal controls, which were accompanied by significant decrease in non enzymatic antioxidants and TAS concentrations. 8-OHdG and cell proliferation level were negatively correlated with non enzymatic antioxidants viz., Vitamin A, Vitamin C, vitamin E level and total antioxidant activity. Altered levels of biomarkers of oxidative stress and cell proliferation activity amongst the malignant, benign and controls suggest a correlation of increased oxidative stress and cell proliferation activity in the progression of disease in breast carcinoma patients. Among the oxidative stress markers and cell proliferation index, decreased level of vitamin A, vitamin C, vitamin E, TAS and increased level of 8-OHdG, cell proliferation index emerged as best predicted biomarkers for subjects with malignancy and benign breast disease.

Relationship between atherogenic index and oxidative stress among patients presented with coronary artery disease in a tertiary care hospital

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2807-2813
Author(s):  
Resmi C R ◽  
Kedari G S R ◽  
Deepa P K
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Density Lipoprotein ◽  
Atherogenic Index ◽  
Enzymatic Antioxidants ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Lipid Parameters ◽  
And Oxidative Stress

CAD is recognized as a multifactorial disease that is influenced by environmental and genetic factors. This study aimed to evaluate the levels of lipid parameters, oxidative stress and antioxidant markers in subjects with CAD compared to their age & sex matched controls and to analyze the relationship between atherogenic Index and oxidative stress among them 62 clinically proved CAD patients and 62 healthy age and sex matched subjects without CAD were selected for this study. 5 ml of fasting venous blood was collected from all the subjects and investigations such as FPG, lipid profile, oxidative markers Malondialdehyde (MDA), F2 isoprostanes (F2iso) and antioxidants glutathione S-transferase (GST), superoxide dismutase (SOD), vitamin-C, vitamin-E were performed. This study showed that levels of lipid parameters total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c) and AI were significantly higher whereas high density lipoprotein cholesterol (HDL-c) were significantly low in CAD patients compared to normal controls. Oxidative stress markers MDA and F2 Isoprostanes level were significantly high, whereas enzymatic antioxidants GST and SOD and non-enzymatic antioxidants Vitamin-C and Vitamin-E levels were significantly low in CAD patients. Oxidative stress markers were found to significantly influence the AI. Results of this study showed that oxidative stress markers F2iso and MDA and antioxidants GST, VIT-C and VIT-E are found to influence the atherogenic index significantly.

scholarly journals The antimalaria drug artemisinin displays strong cytotoxic effect on leukaemia lymphocytes in combination with vitamin C and pro-vitamin K3

2021 ◽  
Vol 24 (4) ◽  
pp. 533-543
Author(s):  
D. Ivanova ◽  
Z. Yaneva ◽  
R. Bakalova R. Bakalova ◽  
S. Semkova ◽  
Zh. Zhelev
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Vitamin C ◽  
Cancer Cells ◽  
Jurkat Cells ◽  
Cell Counting ◽  
Triple Combination ◽  
Vitamin K3 ◽  
Cell Proliferation Activity ◽  
Proliferation Activity

This study investigated the anticancer effect of the anti-parasitic drug artemisinin in combination with two redox modulators: vitamin C and pro-vitamin K3 (C/K3) The experiments were conducted on leukaemia cells Jurkat. Cells were treated with either artemisinin or C/K3 alone and with all three compounds. Cell proliferation and viability were analysed using trypan blue stating and automated cell counting. The results showed that artemisinin (>10 mM) suppressed cell proliferation activity, but did not induce cell death up to 500 mM. The drug demonstrated a clear cytostatic effect at concentrations 250- 500 mM – Jurkat cells did not proliferate, but were alive. The combination C/K3 (200:2, 300:3 mM/mM) applied alone did not affect cell proliferation and viability. Vitamins C/K3 in concentration ratio 500:5 (μM/mM) decreased cell proliferation activity by ~10%. The triple combination artemisinin/C/K3 manifested synergistic anti-proliferative effects at all concentration ratios analysed. This synergistic effect increased with increasing C/K3 concentration. Based on literature data, it was assumed that the anti-proliferative effect of the triple combination was mediated by changes in the redox-homeostasis of cancer cells. The C/K3 redox system likely acted on cancer mitochondria and increased superoxide production and activation of pro-apoptotic signals, specific for cancer cells. On the other hand, artemisinin could generate hydroxyl radicals as a result of activation of Fenton reactions, depleting intracellular reducing equivalents. Both redox mechanisms lead to activation of signal pathways for induction of cancer cell death.

scholarly journals Association of Antioxidant to the Genesis of Psychiatric Disorder

2021 ◽  
Vol 12 (1) ◽  
pp. 588-593
Author(s):  
Ranjit S. Ambad ◽  
Sonal Muley ◽  
Lata Kanyal Butola ◽  
Ajinkya S. Ghogare
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Cell Injury ◽  
Psychiatric Patients ◽  
Enzymatic Antioxidants ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Wide Range ◽  
Before And After

Mental disorders were associated with a wide range of chronic illnesses, disability, and even mortality, particularly among elderly people. Depression will be the second cause of disease. Anxiety is an emotional state of antipathy in which the sense of fear is disproportionate to the magnitude of the risk. Enzymatic antioxidants like SOD, GPx, and non-enzymatic antioxidants such as vitamin C, E act as free scavengers, thereby reducing oxidative stress and resulting in cell injury. Thus, we aimed to study SOD, GPx, Vitamin C, and Vitamin E, 1. To study levels of SOD, GPx, Vitamin C, and Vitamin E in psychiatric disorders.2. To study the levels of Vit-C and E before and after vitamin supplementations. To correlate the levels of SOD, GPx, Vitamin C, and Vitamin E between psychiatric patients and healthy controls (age-matched) attending AVBRH Wardha and SMHRC Nagpur. This cross-sectional examination was completed on 50 psychiatric patients and 50 healthy controls and the levels of SOD, GPx, Vitamin C, and Vitamin E are measured before and after giving supplements. In Psychiatric patients, Superoxide dismutase (SOD) levels were 135.26±24.68, Glutathione Peroxidase levels were 1.591±3.35, Vitamin C levels were 0.32±0.11 and Vitamin E levels were 4.302±1.54, which is lower than the normal range. The present study concludes that antioxidant plays a major role to fight against oxidative stress. So proper antioxidant should be taken.

Effects of exogenous vitamins A, C, and E and NADH supplementation on proliferation, cytokines release, and cell redox status of lymphocytes from healthy aged subjects

2017 ◽  
Vol 42 (6) ◽  
pp. 579-587 ◽  
Author(s):  
Samia Bouamama ◽  
Hafida Merzouk ◽  
Amel Medjdoub ◽  
Amel Merzouk-Saidi ◽  
Sid Ahmed Merzouk
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Vitamin E ◽  
Vitamin C ◽  
The Elderly ◽  
Redox Status ◽  
Age Related ◽  
Immune Alterations ◽  
Cell Redox Status

Aging is an inevitable biological event that is associated with immune alterations. These alterations are related to increased cellular oxidative stress and micronutrient deficiency. Antioxidant supplementation could improve these age-related abnormalities. The aim of this study was to determine in vitro effects of vitamin A, vitamin C, vitamin E, and nicotinamide adenine dinucleotide (NADH) on T cell proliferation, cytokine release, and cell redox status in the elderly compared with young adults. Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were cultured in vitro and stimulated with concanavalin A in the presence or absence of vitamins. Cell proliferation was determined by conducting MTT assays, and based on interleukin-2 and interleukin-4 secretions. Cell oxidant/antioxidant balance was assessed by assaying reduced glutathione (GSH), malondialdehyde, carbonyl protein levels, and catalase activity. The present study demonstrated that T-lymphocyte proliferation was decreased with aging and was associated with cytokine secretion alterations, GSH depletion, and intracellular oxidative stress. In the elderly, vitamin C, vitamin E, and NADH significantly improved lymphocyte proliferation and mitigated cellular oxidative stress, whereas vitamin A did not affect cell proliferation or cell redox status. In conclusion, vitamin C, vitamin E, and NADH supplementation improved T-lymphocytes response in the elderly, and could contribute to the prevention of age-related immune alterations. Consumption of food items containing these vitamins is recommended, and further investigation is necessary to evaluate the effect of vitamin supplementation in vivo.

Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis

1999 ◽  
Vol 188 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Satu-Leena Sallinen ◽  
Pauli K. Sallinen ◽  
Juha T. Kononen ◽  
Kirsi M. Syrj�koski ◽  
Nina N. Nupponen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cyclin D1 ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Patient Prognosis

scholarly journals Oxidative Stress in Preterm Neonates: An Analysis of Oxidative Stress Biomarkers and Antioxidant Profiles

2020 ◽  
Author(s):  
Shobha S Pajai ◽  
Apurva P Bezalwar
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Cord Blood ◽  
Defense Mechanism ◽  
Preterm Neonates ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Study Results ◽  
Preterm Babies

Introduction: Oxidative stress is a complex event determined genetically and induced by an in- utero stressor. Oxidants are composed of reactive free radicals like Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) which are manifested by several macromolecules of lipid, protein and DNA, causing deleterious effects in several organs. Antioxidant defense mechanism and its ability to be induced by hyperoxia is relatively impaired in preterm neonates. Aim: To study oxidative stress and antioxidants in preterm neonates. Materials and Methods: This study is an observational analytical study, which included preterm babies (25 males and 20 females) delivered vaginally from October 2012 to October 2013. Cord blood was collected in citrate bulbs immediately after vaginal delivery and stored at 4°C until processed. Malondialdehyde (MDA), Nitrates, Vitamin C and Vitamin E, levels were measured in cord blood. Statistical z-test was applied. Results: High levels of oxidative stress biomarkers like MDA and Nitrites along with decreased levels of antioxidants, Vitamin C and Vitamin E in preterm neonates was observed. MDA and Nitrates levels were significantly higher in males (p<0.05) than females. Vitamin C and Vitamin E levels were not significant (p>0.05) in both. Conclusion: This study results may conclude that preterm neonates have more oxidative stress especially in males affecting their life survival.

scholarly journals The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

1970 ◽  
Vol 7 (3) ◽  
pp. 14-19 ◽  
Author(s):  
Hekdin Marsius Sipayung ◽  
Jansen Silalahi ◽  
Yuandani Y
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Protein Expression ◽  
Scratch Wound ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Cox 2 ◽  
Nih 3T3 ◽  
Vegf Protein

Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use. Keywords: Rhizomucor miehei, viability, proliferation, migration, expression

Sign in / Sign up

Export Citation Format

Share Document