scholarly journals First case of Conyza canadensis from Hungary with multiple resistance to glyphosate and flazasulfuron

2018 ◽  
Author(s):  
Candelario Palma-Bautista ◽  
Behroz Khalil Tahmasebi ◽  
Pablo Tomás Fernández-Moreno ◽  
Antonia María Rojano-Delgado ◽  
Ricardo Alcántara de la Cruz ◽  
...  
Keyword(s):  
Dose Response ◽  
Shikimic Acid ◽  
Multiple Resistance ◽  
Conyza Canadensis ◽  
Herbicide Application ◽  
Susceptible Population ◽  
First Case ◽  
Acid Accumulation ◽  
Agricultural Areas ◽  
Als Inhibitor

Conyza canadensis is a species invading large agricultural areas throughout the world, mainly to its ability to evolve herbicide resistance. Specifically, in Hungary, extensive areas have been infested by this species due to the difficulty in controlling it with glyphosate. To corroborate this fact as resistance and not as an incorrect herbicide application, eight suspicious glyphosate-resistant C. canadensis populations from different Hungarian regions were studied. In dose-response assays with glyphosate, the LD50 and GR50 values indicated that populations 1 to 5 were resistant to this herbicide (H-5 population the most resistant). Besides, the shikimic acid accumulation tests corroborated the results observed in the dose-response assays. 11 alternative herbicides from 6 different mode of action (MOA) were applied at field doses as control alternatives on populations H-5 and H-6 (both in the same regions). The H-5 population showed an unexpected resistance to flazasulfuron (ALS-inhibitor). The ALS enzyme activity studies indicated that the I50 for H-5 was 63.3 fold higher compared to its correspondent susceptible population (H-6). Therefore, the H-5 population exhibited multiple-resistance to flazasulfuron and glyphosate, being the first case reported in Europe for this two MOA. For that reason, the other herbicides with different MOA have to be tested here.

scholarly journals First Case of Conyza canadensis from Hungary with Multiple Resistance to Glyphosate and Flazasulfuron

Agronomy ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 157 ◽  
Author(s):  
Candelario Palma-Bautista ◽  
Behroz Khalil Tahmasebi ◽  
Pablo Tomás Fernández-Moreno ◽  
Antonia M. Rojano-Delgado ◽  
Ricardo Alcántara de la Cruz ◽  
...  
Keyword(s):  
Dose Response ◽  
Shikimic Acid ◽  
Multiple Resistance ◽  
Conyza Canadensis ◽  
Poor Control ◽  
Susceptible Population ◽  
First Case ◽  
Whole Plant ◽  
Acid Accumulation ◽  
Als Inhibitor

Conyza canadensis is a species invading large areas throughout the world, mainly due to its ability to evolve herbicide resistance. In Hungary, extensive areas have been infested by this species due to the difficulty in controlling it with glyphosate. To determine whether poor control was a result of misapplication or glyphosate resistance, eight suspected glyphosate-resistant C. canadensis populations from different Hungarian regions were studied. In whole-plant dose-response assays with glyphosate, the LD50 and GR50 values (survival and fresh weight reduction at 50% relative to the untreated control, respectively) indicated that resistance was confirmed in five of the eight populations (H-5 population being the most resistant). Additionally, the shikimic acid accumulation tests corroborated the results observed in the dose–response assays. 11 alternative herbicides from six different modes of action (MOA) were applied at field doses as control alternatives on populations H-5 and H-6 (both in the same regions). The H-5 population showed an unexpected resistance to flazasulfuron (ALS-inhibitor). The ALS enzyme activity studies indicated that the I50 for H-5 with flazasulfuron was 63.3 times higher compared to its correspondent susceptible population (H-6). Therefore, the H-5 population exhibited multiple-resistance to flazasulfuron and glyphosate, being the first case reported in Europe for these two MOA.

scholarly journals First Report of Amaranthus hybridus with Multiple Resistance to 2,4-D, Dicamba, and Glyphosate

Agronomy ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 140 ◽  
Author(s):  
Ignacio Dellaferrera ◽  
Eduardo Cortés ◽  
Elisa Panigo ◽  
Rafael De Prado ◽  
Pedro Christoffoleti ◽  
...  
Keyword(s):  
Dose Response ◽  
Shikimic Acid ◽  
Multiple Resistance ◽  
Agricultural Systems ◽  
Amaranthus Hybridus ◽  
First Report ◽  
Resistant Population ◽  
Acid Accumulation ◽  
Invasive Capacity

In many countries, Amaranthus hybridus is a widespread weed in agricultural systems. The high prolificacy and invasive capacity as well as the resistance of some biotypes to herbicides are among the complications of handling this weed. This paper reports on the first A. hybridus biotypes with resistance to auxinic herbicides and multiple resistance to auxinic herbicides and the EPSPs inhibitor, glyphosate. Several dose response assays were carried out to determine and compare sensitivity of six population of A. hybridus to glyphosate, 2,4-D, and dicamba. In addition, shikimic acid accumulation and piperonil butoxide effects on 2,4-D and dicamba metabolism were tested in the same populations. The results showed four populations were resistant to dicamba and three of these were also resistant to 2,4-D, while only one population was resistant to glyphosate. The glyphosate-resistant population also showed multiple resistance to auxinic herbicides. Pretreatment with piperonil butoxide (PBO) followed by 2,4-D or dicamba resulted in the death of all individual weeds independent of herbicide or population.

scholarly journals Sensitivity of Digitaria insularis to herbicides in agricultural areas, in the Brazilian Cerrado biome

2020 ◽  
Vol 55 ◽  
Author(s):  
Núbia Maria Correia ◽  
Pedro Eduardo Rampazzo ◽  
Lucas da Silva Araújo ◽  
Caio Vitagliano Santi Rossi
Keyword(s):  
Mineral Oil ◽  
Cross Resistance ◽  
Multiple Resistance ◽  
Brazilian Cerrado ◽  
Herbicide Application ◽  
Plant Populations ◽  
Susceptible Population ◽  
Cerrado Biome ◽  
Different Populations ◽  
Agricultural Areas

Abstract: The objective of this work was to evaluate the sensitivity of different populations of Digitaria insularis to the glyphosate, clethodim, and haloxyfop-P-methyl herbicides, in agricultural areas, and to develop infestation maps based on the responses of these populations. One hundred sixty-one populations suspected of being resistant were evaluated and compared to a susceptible population. When plants displayed three to four tillers, the populations were sprayed with glyphosate (1,000 g ha-1 a.e.), clethodim (108 g ha-1 a.i. + 0.5% mineral oil), and haloxyfop-P-methyl (62.35 g ha-1 a.i. + 0.5% mineral oil); plants without herbicide application were used as the control. The plant populations were classified as susceptible, intermediately resistant (with susceptible and resistant plants), or resistant to the tested herbicides. All populations were susceptible to clethodim; 97.5% were susceptible and 2.5% were intermediately resistant to haloxyfop-P-methyl; and 9.9% were susceptible, 21.1% intermediately resistant, and 68.9% resistant to glyphosate. Glyphosate-resistant populations are homogeneously distributed throughout the evaluated regions. There are no cases of D. insularis multiple resistance in the sampled regions; however, cross-resistance to glyphosate and haloxyfop-P-methyl was detected.

scholarly journals Confirmation of Multiple Resistant Chloris radiata Population, Harvested in Colombian Rice Fields

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 496
Author(s):  
Verónica Hoyos ◽  
Guido Plaza ◽  
José G. Vázquez-Garcia ◽  
Candelario Palma-Bautista ◽  
Antonia M. Rojano-Delgado ◽  
...  
Keyword(s):  
Dose Response ◽  
Weed Management ◽  
Shikimic Acid ◽  
Lethal Dose ◽  
Dry Weight ◽  
Integrated Weed Management ◽  
Cross Resistance ◽  
Multiple Resistance ◽  
Visual Evaluation ◽  
Acid Accumulation

This paper reports the first C. radiata population with resistance to glyphosate and multiple resistance to the acetolactate synthase (ALS) inhibitor, imazamox. Two populations, one putative resistant (R) and one susceptible (S), were used in the studies. Dose–response experiments were performed to evaluate the resistance factor (RF). Shikimic acid accumulation, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and ALS enzyme activities were studied together with chemical integrated weed management (adjuvants and alternative herbicides). The resistance to glyphosate and imazamox was confirmed based on the dry weight reduction, visual evaluation and survival. The results of dose–response curve assays showed for the R population intermedium RF for glyphosate (5.1 and 9.7 for amount of herbicide needed to reduce the dry weight by 50% GR50 and lethal dose of 50% LD50, respectively) and high RF for imazamox (34.9 and 37.4, respectively). The low shikimic acid accumulation in R population confirmed the glyphosate resistance. The glyphosate concentration which inhibited the EPSPS enzyme in 50% (I50) was approximately 20 times higher for R population than the S population, while the imazamox I50 in ALS enzyme for the R plants was 89 times greater than the S plants. In the chemical integrated weed management, the foliar retention and effectivity assays showed that the use of adjuvants improves the retention of glyphosate and imazamox, and the reduction in dry weight of weeds. The alternative herbicides study showed that the acetyl-CoA carboxylase (ACCase) inhibitors, paraquat and glufosinate, had better results for control in this species. However, poor control was observed with bispyribac-sodium, metsulfuron-methyl and quinclorac, indicating possible cross-resistance for ALS-inhibitors and also multiple resistance for auxinic herbicides (quinclorac). Nevertheless dose–response experiments are required to confirm this assumption.

scholarly journals New Case of False-Star-Grass (Chloris distichophylla) Population Evolving Glyphosate Resistance

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 377 ◽  
Author(s):  
José G. Vázquez-García ◽  
Sajedeh Golmohammadzadeh ◽  
Candelario Palma-Bautista ◽  
Antonia M. Rojano-Delgado ◽  
José A. Domínguez-Valenzuela ◽  
...  
Keyword(s):  
Weed Management ◽  
Chemical Control ◽  
Shikimic Acid ◽  
Dry Weight ◽  
Glyphosate Resistance ◽  
Integrated Weed Management ◽  
Resistance Level ◽  
Susceptible Population ◽  
First Case

Chloris distichophylla, suspected of glyphosate resistance (GR), was collected from areas of soybean cultivation in Rio Grande do Sul, Brazil. A comparison was made with a susceptible population (GS) to evaluate the resistance level, mechanisms involved, and control alternatives. Glyphosate doses required to reduce the dry weight (GR50) or cause a mortality rate of 50% (LD50) were around 5.1–3 times greater in the GR population than in the GS population. The shikimic acid accumulation was around 6.2-fold greater in GS plants than in GR plants. No metabolized glyphosate was found in either GR or GS plants. Both populations did not differ in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) basal activity or in vitro inhibition of EPSPS activity by glyphosate (I50). The maximum glyphosate absorption was observed at 96 hours after treatment (HAT), which was twofold higher in the GS plants than in the GR plants. This confirms the first case of glyphosate resistance in C. distichophylla. In addition, at 96 HAT, the GS plants translocated more 14C-glyphosate than the GR ones. The best options for the chemical control of both C. distichophylla populations were clethodim, quizalofop, paraquat, glufosinate, tembotrione, diuron, and atrazine. The first case of glyphosate resistance in C. distichophylla was due to impaired uptake and translocation. Chemical control using multiple herbicides with different modes of action (MOA) could be a tool used for integrated weed management (IWM) programs.

scholarly journals Multiple Resistance to Glyphosate and 2,4-D in Carduus acanthoides L. from Argentina and Alternative Control Solutions

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1735
Author(s):  
Candelario Palma-Bautista ◽  
Pablo Belluccini ◽  
Valentin Gentiletti ◽  
José G. Vázquez-García ◽  
Hugo E. Cruz-Hipolito ◽  
...  
Keyword(s):  
Dose Response ◽  
Shikimic Acid ◽  
Multiple Resistance ◽  
Response Curves ◽  
Retention Capacity ◽  
Carduus Acanthoides ◽  
Action Mechanisms ◽  
North And South America ◽  
North And South ◽  
Different Doses

Carduus acanthoides L. is an invasive species native to Europe and distributed in other parts of the world, including North and South America. In Cordoba, Argentina, control failures of this species have been reported in Roundup Ready (RR) soybean crops where glyphosate and 2,4-D have frequently been applied, although there are no confirmed reports worldwide of resistance to glyphosate and 2,4-D in this species. Dose–response tests showed multiple-resistance to both active principles. The resistant population (R) had LD50 values of 1854.27 and 1577.18 g ae ha−1 (grams of acid equivalent per hectare), while the susceptible (S) population had LD50 values of 195.56 and 111.78 g ae ha−1 for glyphosate and 2,4-D, respectively. Low accumulations of shikimic acid (glyphosate) and ethylene (2,4-D) at different doses in the R population compared to the S population support the results observed in the dose–response curves. No significant differences in leaf retention were observed for glyphosate and 2,4-D in the R and S populations. However, the use of adjuvants increased the retention capacity of herbicides in both populations. Ten alternative herbicides with seven different action mechanisms (MOAs) were evaluated and the most effective active principles were dicamba, bromoxynil, atrazine, tembotrione, flazasulfuron, glufosinate, and paraquat. These findings are the first evidence of glyphosate and 2,4 D resistance in C. acanthoides.

Detection of Sourgrass (Digitaria insularis) Biotypes Resistant to Glyphosate in Brazil

Weed Science ◽  
2011 ◽  
Vol 59 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Leonardo Bianco de Carvalho ◽  
Hugo Cruz-Hipolito ◽  
Fidel González-Torralva ◽  
Pedro Luis da Costa Aguiar Alves ◽  
Pedro Jacob Christoffoleti ◽  
...  
Keyword(s):  
Contact Angle ◽  
Dose Response ◽  
Shikimic Acid ◽  
Glyphosate Resistance ◽  
Resistance Factor ◽  
Natural Area ◽  
Perennial Weed ◽  
Perennial Crops ◽  
Spray Droplets ◽  
Acid Accumulation

Sourgrass is a perennial weed infesting annual and perennial crops in Brazil. Three biotypes (R1, R2, and R3) of sourgrass suspected to be glyphosate-resistant (R) and another one (S) from a natural area without glyphosate application, in Brazil, were tested for resistance to glyphosate based on screening, dose-response, and shikimic acid assays. Both screening and dose-response assays confirmed glyphosate resistance in the three sourgrass biotypes. Dose-response assay indicated a resistance factor of 2.3 for biotype R1 and 3.9 for biotypes R2 and R3. The hypothesis of a glyphosate resistance was corroborated on the basis of shikimic acid accumulation, where the S biotype accumulated 3.3, 5.0, and 5.7 times more shikimic acid than biotypes R1, R2, and R3, respectively, 168 h after treatment with 157.50 g ae ha−1of glyphosate. There were no differences in contact angle of spray droplets on leaves and spray retention, indicating that differential capture of herbicide by leaves was not responsible for resistance in these biotypes. The results confirmed resistance of sourgrass to glyphosate in Brazil.

Multiple herbicide–resistant Lolium spp. is prevalent in wheat production in Texas Blacklands

2020 ◽  
Vol 34 (5) ◽  
pp. 652-660 ◽  
Author(s):  
Vijay Singh ◽  
Aniruddha Maity ◽  
Seth Abugho ◽  
James Swart ◽  
David Drake ◽  
...  
Keyword(s):  
Perennial Ryegrass ◽  
Dose Response ◽  
Management Strategies ◽  
Acetolactate Synthase ◽  
Italian Ryegrass ◽  
Susceptible Population ◽  
Wheat Production ◽  
Herbicide Resistant ◽  
Randomized Design ◽  
Als Inhibitor

AbstractField surveys were conducted across the Blacklands region of Texas during 2016 and 2017 to document the distribution of herbicide-resistant Lolium spp. infesting winter wheat production fields in the region. A total of 68 populations (64 Italian ryegrass, four perennial ryegrass) were evaluated in a greenhouse for sensitivity to herbicides of three different modes of action: an acetolactate synthase (ALS) inhibitor (mesosulfuron-methyl), two acetyl-coenzyme-A carboxylase (ACCase) inhibitors (diclofop-methyl and pinoxaden), and a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitor (glyphosate). Herbicides were applied at twice the label-recommended rates for mesosulfuron-methyl (29 g ai ha−1), diclofop-methyl (750 g ai ha−1), and pinoxaden (118 g ai ha−1); and at the recommended rate for glyphosate (868 g ae ha−1). The herbicide screenings were followed by dose-response assays of the most-resistant ryegrass population for each herbicide at eight rates (0.5, 1, 2, 4, 8, 16, 32, and 64×), compared with a susceptible population at six rates (0.0625, 0.125, 0.25, 0.5, 1, and 2×). The initial screening and dose-response experiments were conducted in a completely randomized design with three replications and two experimental runs. Survivors (<80% injury) were characterized as highly resistant (0% to 20% injury) or moderately resistant (21% to 79%). Results showed that 97%, 92%, 39%, and 3% of the Italian ryegrass populations had survivors to diclofop-methyl, mesosulfuron-methyl, pinoxaden, and glyphosate treatments, respectively. Of the four perennial ryegrass populations, three were resistant to diclofop-methyl and mesosulfuron-methyl, and one was resistant to pinoxaden as well. Perennial ryegrass populations did not exhibit any resistance to glyphosate. Dose-response assays revealed 37-, 196-, and 23-fold resistance in Italian ryegrass to mesosulfuron-methyl, diclofop-methyl, and pinoxaden, respectively, compared with a susceptible standard. One Italian ryegrass population exhibited three-way multiple resistance to ACCase-, ALS-, and EPSPS-inhibitors. The proliferation of multiple herbicide–resistant ryegrass is a challenge to sustainable wheat production in Texas Blacklands and warrants diversified management strategies.

First Case of Multiple Resistance to Glyphosate and PPO-inhibiting Herbicides in Rigid Ryegrass (Lolium rigidum) in Spain

Weed Science ◽  
2017 ◽  
Vol 65 (6) ◽  
pp. 690-698 ◽  
Author(s):  
Pablo Tomas Fernandez-Moreno ◽  
Antonia Maria Rojano-Delgado ◽  
Julio Menendez ◽  
Rafael De Prado
Keyword(s):  
Dose Response ◽  
Selection Pressure ◽  
Protoporphyrin Ix ◽  
Glyphosate Resistance ◽  
Resistance Pattern ◽  
Multiple Resistance ◽  
Lolium Rigidum ◽  
First Case ◽  
Natural Tendency ◽  
Rigid Ryegrass

Five rigid ryegrass populations suspected of being resistant to both glyphosate and oxyfluorfen were collected in southern Spain and tested under laboratory-controlled conditions. Four populations (Depuradora, Condado, AlamoRasilla, and Portichuelo) were treated with glyphosate for at least 15 consecutive years, and treatments during the last 5 yr were mixed with oxyfluorfen. The fifth population (4alamos) followed the same glyphosate treatment, although oxyfluorfen was never used to control it. Dose–response assays confirmed glyphosate resistance in all populations, with resistance indexes ranging from 11.7 to 37.5 (GR90). Shikimate accumulation assays consistently supported these data, as the most glyphosate-resistant populations (Depuradora and Condado) displayed the lowest shikimate levels. Surprisingly, four populations (Depuradora, Condado, AlamoRasilla, and Portichuelo) displayed 7.93- to 70.18-fold more resistance (GR90) to oxyfluorfen, despite limited selection pressure, showing a similar resistance pattern as that for glyphosate. The 4alamos population displayed oxyfluorfen GR90values that were similar to those observed in susceptible plants; however, this population was significantly more resistant in terms of plant survival (LD90). Protoporphyrin IX accumulation assays supported the results of dose–response assays, in that the most oxyfluorfen-resistant populations accumulated less protoporphyrin IX. Although more studies are needed, it seems that these five glyphosate-resistant weed populations display a natural tendency to easily develop resistance to oxyfluorfen, with the populations that have higher resistance to glyphosate also having higher resistance to oxyfluorfen.

scholarly journals Confirmation and Control of Annual Bluegrass (Poa annua) with Resistance to Prodiamine and Glyphosate

2017 ◽  
Vol 31 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Shane M. Breeden ◽  
James T. Brosnan ◽  
Thomas C. Mueller ◽  
Gregory K. Breeden ◽  
Brandon J. Horvath ◽  
...  
Keyword(s):  
Dose Response ◽  
Shikimic Acid ◽  
Golf Course ◽  
Multiple Resistance ◽  
Post Dose ◽  
Annual Bluegrass ◽  
Resistant Phenotype ◽  
Dry Biomass ◽  
Herbicide Mixtures ◽  
And Control

Poor annual bluegrass control was reported in golf course roughs following treatment with prodiamine (1120 gaiha−1) and glyphosate (840 gaeha−1) during hybrid bermudagrass dormancy. Research was conducted to determine if this annual bluegrass phenotype was resistant to both prodiamine and glyphosate and to determine the efficacy of herbicide mixtures for controlling this phenotype in the field. In PRE or POST dose-response experiments, 9 to 31 times more prodiamine or glyphosate was needed to control (or reduce dry biomass of) this resistant phenotype by 50% compared to an herbicide-susceptible phenotype. Moreover, glyphosate-susceptible plants accumulated 50% more shikimic acid (898 mgkg−1) 6 d after treatment than those resistant to glyphosate (394 mgkg−1). October (fall) applications of herbicide mixtures containing trifloxysulfuron, simazine,S-metolachlor, or mesotrione controlled this resistant annual bluegrass phenotype 84 to 98% in April (spring), with no differences detected among treatments. Our findings document the second instance of annual bluegrass evolving multiple resistance in a managed turfgrass system. However, several herbicide mixtures can be used to effectively manage this resistant phenotype.

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