Evidence of a Possible Double Hybrid Origin for Two Malagasy Species of Piper L. (Piperaceae)

two new species of genus Piper L. from Madagascar: Piper malgassicum and Piper tsarasotrae, were analyzed to investigate their phylogenetic position and evolutionary history. Both plastidial and nuclear markers were used for sequencing. The plastidial markers (ndhF and the trnL intron) showed a close relationship between the two species with respect to the other species of Piper. Both species appeared phylogenetically related to the African P. guineense and the Malagasian/Mascarenhas endemic P. borbonense. The nuclear marker (G3PDH) amplification produced two separate sets of sequences: “long” sequences, that could be easily translated into an amino acid chain, and “short” sequences, characterized by deletions that did not allowed to translate them correctly to an amino acid sequence. Analyzing together the nuclear sequences, we observed that the “long” sequence of P. tsarasotrae had a stricter relationship to the African accessions of P. guineense, while the accession of P. malgassicum was more strictly related to P. borbonense. On the contrary both “short” sequences of Piper malgassicum and Piper tsaratsotrae resulted phylogenetically related to Asian accessions and more distantly related to the formerly cited species. This unexpected result was tentatively explained with a more ancient hybridization event between an ancestor of P. malgassicum and P. tsarasotrae (and possibly P. borbonense) and an Asian species of Piper. The Asian contribution would have produced the ancestors of the “short” sequences that would eventually have lost functionality by deletions, becoming paralogs. A more recent hybridization event would have led to the separation of Piper malgassicum from Piper tsarasotrae with an African pollen-derived genome contribution from P. guineense or, more probably, an ancestor thereof, to an ancestor of P. tsarasotrae. The chromosome numbers of P. tsarasotrae (2n = about 38) and P. malgassicum (2n = about 46), were more like the Asian species than to the American species. Unfortunately, no chromosome number of the African species P. guineense is currently available, to analyze eventual chromosomal connections.

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The Phylogenetic Position of Vincetoxicum pannonicum (Borhidi) Holub Supports the Species' Allopolyploid Hybrid Origin

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.8931 ◽

2020 ◽

Vol 89 (3) ◽

Cited By ~ 1

Author(s):

Orsolya Horváth ◽

Levente Laczkó ◽

Zsuzsa Lisztes-Szabó ◽

Attila Molnár V. ◽

Agnieszka Popiela ◽

...

Keyword(s):

Genome Size ◽

Hybrid Origin ◽

Phylogenetic Position ◽

Gene Trees ◽

Separate Species ◽

Diploid Parent ◽

Hybridization Event ◽

Pollen Donor ◽

Molecular Phylogenetic ◽

Allotetraploid Hybrid

The Pannonian endemic species Vincetoxicum pannonicum was described from specimens collected in Hungary and occurs at only few locations. It is considered “vulnerable” according to the International Red List. The chromosome set was reported to be tetraploid, and the species was hypothesized to be an allotetraploid hybrid of the Balkan species V. fuscatum and the Adriatic species hirundinaria subsp. adriaticum. We investigated the origin of V. pannonicum using molecular phylogenetic methods by separately analyzing the multicopy nuclear ribosomal internal transcribed spacer (nrITS) and the plastid-encoded trnH-psbA DNA regions and by evaluating discrepancies between the produced gene trees. Paralogs in the nrITS region clustered in two main groups, one of which was closest to V. fuscatum, and the other included V. hirundinaria subsp. adriaticum. According to trnH-psbA sequences, V. pannonicum and V. hirundinaria subsp. adriaticum formed a single group. Our results show that V. pannonicum diversified because of hybrid speciation, in which V. fuscatum was the pollen donor. We discovered a similar placement of V. maeoticum, which suggests a further hybridization event between V. fuscatum and a species of the V. hirundinaria group. Our genome-size estimate indicates almost sixfold larger genome size in V. pannonicum compared to the maternal diploid parent, suggesting hexaploidy; however, V. pannonicum is tetraploid. This may suggest cytological diploidization in the allopolyploid V. pannonicum. We observed substantial genetic distance between V. hirundinaria subsp. adriaticum and all other subspecies of V. hirundinaria, and we therefore propose that V. adriaticum should be regarded as a separate species.

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Resolving the Lourinia armata (Claus, 1866) complex with remarks on the monophyletic status of Louriniidae, Monard 1927 (Copepoda: Harpacticoida)

Zootaxa ◽

10.11646/zootaxa.5051.1.15 ◽

2021 ◽

Vol 5051 (1) ◽

pp. 346-386

Author(s):

SÜPHAN KARAYTUĞ ◽

SERDAR SAK ◽

ALP ALPER ◽

SERDAR SÖNMEZ

Keyword(s):

Comparative Evaluation ◽

Species Complex ◽

Detailed Examination ◽

The Other ◽

Phylogenetic Position ◽

Nicobar Island ◽

Other Hand ◽

The Family ◽

Close Relationship ◽

Almost All

An attempt was made to test if Lourinia armata (Claus, 1866)—as it is currently diagnosed—represents a species complex. Detailed examination and comparisons of several specimens collected from different localities suggest that L. armata indeed represents a complex of four closely related morphospecies that can be differentiated from one another by only detailed observations. One of the four species is identified as Lourinia aff. armata and the other three species are described as new to science and named as Lourinia wellsi sp. nov., L. gocmeni sp. nov., and L. aldabraensis sp. nov. Detailed review of previous species records indicates that the genus Lourinia Wilson, 1924 is distributed worldwide. Ceyloniella nicobarica Sewell, 1940, originally described from Nicobar Island and previously considered a junior subjective synonym of L. armata is reinstated as Lourinia nicobarica (Sewell, 1940) comb. nov. on the basis of the unique paddle-shaped caudal ramus seta V. It is postulated that almost all of these records are unreliable in terms of representing true Lourinia aff. armata described herein. On the other hand, the comparative evaluation of the illustrations and descriptions in the published literature indicates the presence of several new species waiting to be discovered in the genus Lourinia. It has been determined that, according to updated modern keys, the recent inclusion of the monotypic genus Archeolourinia Corgosinho & Schizas, 2013 in the Louriniidae is not justified since Archeolourinia shermani Corgosinho & Schizas, 2013 does not belong to this family but should be assigned to the Canthocamptidae. On the other hand, it has been argued that the exact phylogenetic position of the Louriniidae still remains problematic since none of the diagnostic characters supports the monophyly of the family within the Oligoarthra. It has also been argued that the close relationship between Louriniidae and Canthocamptidae is supported since both families share the homologous sexual dimorphism (apophysis) on P3 endopod. The most important characteristic that can possibly be used to define Louriniidae is the reduction of maxilliped.

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Morphology of a unique ophidiid, Hypopleuron caninum Radcliffe 1913 (Ophidiiformes, Ophidiidae, Neobythitinae), suggesting a close relationship with the family Carapidae

Zootaxa ◽

10.11646/zootaxa.4521.4.2 ◽

2018 ◽

Vol 4521 (4) ◽

pp. 499

Author(s):

SHINPEI OHASHI

Keyword(s):

Phylogenetic Position ◽

Dorsal Fin ◽

The Family ◽

Close Relationship ◽

Pelvic Fin

Osteological and myological characters of the ophidiid Hypopleuron caninum are described here in detail. In addition to well-known characters of Ophidiidae (e.g., anal-fin origin well posterior to dorsal-fin origin; barbel-like pelvic fin; many scales), many unique or rare conditions were also recognized, including predorsal bone and maxilla condition, and canine teeth on the premaxilla, which were suggestive of a relationship with Carapidae. Furthermore, the predorsal bone appear to be homologous with the vexillar support, a carapid synapomorphy. Although several characters differ from carapid counterparts (e.g., more anterior anal-fin origin and scales absent in Carapidae), some intermediate conditions between Ophidiidae and Carapidae are apparent in Hypopleuron caninum, which may therefore occupy an intermediate phylogenetic position between the two families. A unique morphology of small second infraorbital bone is recognized in all ophidiiform species observed in this study, and it may be a synapomorphic character of the order Ophidiiformes.

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Two new species of Roccellaceae (Ascomycota: Arthoniales) from Brazil, with the description of the new genus Sergipea

The Lichenologist ◽

10.1017/s0024282913000303 ◽

2013 ◽

Vol 45 (5) ◽

pp. 627-634 ◽

Cited By ~ 12

Author(s):

André APTROOT ◽

Damien ERTZ ◽

Edvaneide Leandro de LIMA ◽

Katia Almeida de JESUS ◽

Leonor Costa MAIA ◽

...

Keyword(s):

New Species ◽

New Genus ◽

Phylogenetic Position ◽

Generic Type ◽

Bright Orange ◽

Two New Species ◽

Basal Branch ◽

Close Relationship ◽

Relationship Of ◽

A New Species

AbstractThe new lichen genus Sergipea M. Cáceres, Ertz & Aptroot is described in the Roccellaceae, based on the new species Sergipea aurata M. Cáceres, Ertz & Aptroot from NE Brazil. The species was found in a remnant of Atlantic transition forest in Sergipe. It is similar in many respects to species of the genus Enterographa, but it is characterized by bright orange stromata, due to the presence of an anthraquinone, and a thallus with a somewhat byssoid hypothallus. Phylogenetically it is close to the genera Dichosporidium and Erythrodecton. The phylogenetic position of the generic type of Dichosporidium confirms the close relationship of the genus to Erythrodecton in the basal branch of the Roccellaceae. A new species of Enterographa is also described from NE Brazil. Enterographa rotundata E. L. Lima, M. Cáceres & Aptroot has solitary, round apothecia, which is unusual in this genus with mainly elongated apothecia or punctiform apothecia arranged in lines. It was found in Caatinga forest in Pernambuco.

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Phylogeny and morphology of genus Nephrocytium (Sphaeropleales, Chlorophyceae, Chlorophyta) from China

Phytotaxa ◽

10.11646/phytotaxa.319.1.4 ◽

2017 ◽

Vol 319 (1) ◽

pp. 84 ◽

Cited By ~ 2

Author(s):

XUDONG LIU ◽

HUAN ZHU ◽

BENWEN LIU ◽

GUOXIANG LIU ◽

ZHENGYU HU

Keyword(s):

Phylogenetic Analyses ◽

Light And Electron Microscopy ◽

Taxonomic Position ◽

Phylogenetic Position ◽

Phytoplankton Communities ◽

Close Phylogenetic Relationship ◽

The Family ◽

Close Relationship ◽

Molecular Phylogenetic

The genus Nephrocytium Nägeli is a common member of phytoplankton communities that has a distinctive morphology. Its taxonomic position is traditionally considered to be within the family Oocystaceae (Trebouxiophyceae). However, research on its ultrastructure is rare, and the phylogenetic position has not yet been determined. In this study, two strains of Nephrocytium, N. agardhianum Nägeli and N. limneticum (G.M.Smith) G.M.Smith, were identified and successfully cultured in the laboratory. Morphological inspection by light and electron microscopy and molecular phylogenetic analyses were performed to explore the taxonomic position. Ultrastructure implied a likely irregular network of dense and fine ribs on the surface of the daughter cell wall that resembled that of the genus Chromochloris Kol & Chodat (Chromochloridaceae). Phylogenetic analyses revealed that Nephrocytium formed an independent lineage in the order Sphaeropleales (Chlorophyceae) with high support values and a close phylogenetic relationship with Chromochloris. Based on combined morphological, ultrastructural and phylogenetic data, we propose a re-classification of Nephrocytium into Sphaeropleales, sharing a close relationship with Chromochloris.

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The genetic analysis of new Polish strains of European brown hare syndrome virus

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0048 ◽

2014 ◽

Vol 17 (2) ◽

pp. 353-355

Author(s):

E. Kwit ◽

M. Chrobocińska ◽

Z. Grądzki ◽

Ł. Jarosz ◽

B. Majer-Dziedzic ◽

...

Keyword(s):

Genetic Analysis ◽

Phylogenetic Position ◽

Brown Hare ◽

Gene Encoding ◽

European Brown Hare ◽

Close Relationship ◽

Provinces Of Poland ◽

Pcr Method ◽

European Brown Hare Syndrome ◽

Hare Population

Abstract In this paper we describe recently occurring outbreaks of European brown hare syndrome (EBHS) in a captive hare population. The aim of our study was to evaluate the phylogenetic position of detected Polish strains compared to other European strains of EBHSV. Investigations were undertaken in hares from different provinces of Poland. Liver or spleen samples were tested for viral RNA using the RT-nested PCR method and the products were subsequently sequenced. The genetic analysis was based on the fragment of gene encoding viral capsid protein; it revealed a high homology and close relationship between Polish and European EBHSV strains isolated between 2001 and 2011

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Cell swelling increases bile flow and taurocholate excretion into bile in isolated perfused rat liver

Biochemical Journal ◽

10.1042/bj2810593 ◽

1992 ◽

Vol 281 (3) ◽

pp. 593-595 ◽

Cited By ~ 23

Author(s):

C Hallbrucker ◽

F Lang ◽

W Gerok ◽

D Häussinger

Keyword(s):

Bile Acid ◽

Amino Acid ◽

Cell Volume ◽

Rat Liver ◽

Bile Flow ◽

Cell Swelling ◽

Isolated Perfused Rat Liver ◽

Perfused Rat Liver ◽

Volume Changes ◽

Close Relationship

The effects of aniso-osmotically and amino-acid-induced cell-volume changes on bile flow and biliary taurocholate excretion were studied in isolated perfused rat liver. With taurocholate (100 microM) in the influent perfusate, hypo-osmotic exposure (225 mosmol/l) increased taurocholate excretion into bile and bile flow by 42 and 27% respectively, whereas inhibition by 32 and 47% respectively was observed after hyperosmotic (385 mosmol/l) exposure. The effects of aniso-moticity on taurocholate excretion into bile was observed throughout aniso-osmotic exposure, even after completion of volume-regulatory ion fluxes and were fully reversible upon re-exposure to normo-osmotic media. Hypo-osmotic cell swelling (225 mosmol/l) increased the Vmax. of taurocholate translocation from the sinusoidal compartment into bile about 2-fold. Also, cell swelling induced by glutamine and glycine stimulated both bile flow and biliary taurocholate excretion. There was a close relationship between the aniso-osmotically and amino-acid-induced change of cell volume and taurocholate excretion into bile. The data suggest that liver cell volume plays an important role in regulating bile-acid-dependent bile flow and biliary taurocholate excretion.

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Biological, antigenic and phylogenetic characterization of the flavivirus Alfuy

Journal of General Virology ◽

10.1099/vir.0.81252-0 ◽

2006 ◽

Vol 87 (2) ◽

pp. 329-337 ◽

Cited By ~ 26

Author(s):

Fiona J. May ◽

Mario Lobigs ◽

Eva Lee ◽

Debra J. Gendle ◽

John S. Mackenzie ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Amino Acid ◽

Distinct Species ◽

Prototype Strain ◽

Glycosylation Site ◽

Encephalitis Virus ◽

Antigenic Relationship ◽

Entire Genome ◽

Phylogenetic Characterization ◽

Close Relationship

Alfuy virus (ALFV) is classified as a subtype of the flavivirus Murray Valley encephalitis virus (MVEV); however, despite preliminary reports of antigenic and ecological similarities with MVEV, ALFV has not been associated with human disease. Here, it was shown that ALFV is at least 104-fold less neuroinvasive than MVEV after peripheral inoculation of 3-week-old Swiss outbred mice, but ALFV demonstrates similar neurovirulence. In addition, it was shown that ALFV is partially attenuated in mice that are deficient in α/β interferon responses, in contrast to MVEV which is uniformly lethal in these mice. To assess the antigenic relationship between these viruses, a panel of monoclonal antibodies was tested for the ability to bind to ALFV and MVEV in ELISA. Although the majority of monoclonal antibodies recognized both viruses, confirming their antigenic similarity, several discriminating antibodies were identified. Finally, the entire genome of the prototype strain of ALFV (MRM3929) was sequenced and phylogenetically analysed. Nucleotide (73 %) and amino acid sequence (83 %) identity between ALFV and MVEV confirmed previous reports of their close relationship. Several nucleotide and amino acid deletions and/or substitutions with putative functional significance were identified in ALFV, including the abolition of a conserved glycosylation site in the envelope protein and the deletion of the terminal dinucleotide 5′-CUOH-3′ found in all other members of the genus. These findings confirm previous reports that ALFV is closely related to MVEV, but also highlights significant antigenic, genetic and phenotypic divergence from MVEV. Accordingly, the data suggest that ALFV is a distinct species within the serogroup Japanese encephalitis virus.

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Two new Oxalis (Oxalidaceae) species, including 5 varieties, from the Richtersveld of South Africa

Phytotaxa ◽

10.11646/phytotaxa.314.2.4 ◽

2017 ◽

Vol 314 (2) ◽

pp. 231

Author(s):

LÉANNE L. DREYER ◽

FRANCOIS ROETS ◽

KENNETH C. OBERLANDER

Keyword(s):

New Species ◽

Its Region ◽

Morphological Characters ◽

Phylogenetic Position ◽

African Species ◽

Widespread Species ◽

Taxonomic Descriptions ◽

Granite Outcrops ◽

Nuclear Its ◽

Typical Variety

Two new species of Oxalis (Oxalidaceae) from the Richtersveld are described, namely Oxalis canaliculata and O. magnifolia. These widespread species are morphologically distinct and easily identified, but both contain populations in which individuals consistently differ morphologically from typical varieties. The typical variety of Oxalis canaliculata is widespread throughout the Richtersveld, while O. canaliculata var. trifoliolata is known from a single population and O. canaliculata var. graniticola is known only from granite outcrops. Oxalis magnifolia includes the typical variety that is widespread and Oxalis magnifolia var. reduplicata, which is only known from two populations in close proximity to one another. Morphological characters, preliminary phylogenetic position based on data from the nuclear ITS region, habitat description and formal taxonomic descriptions are provided for all taxa. The diagnostic characters of the new species are compared to all phenotypically similar southern African species.

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