scholarly journals Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of extracts and Evaluation of Antifungal Activity.

2020 ◽  
Author(s):  
Antonio Bernabé-Antonio ◽  
Alejandro Sánchez-Sánchez ◽  
Antonio Romero-Estrada ◽  
Juan Carlos Meza-Contreras ◽  
José Antonio Silva-Guzmán ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Naphthaleneacetic Acid ◽  
Leaf Extracts ◽  
Sclerotium Cepivorum ◽  
Friable Callus ◽  
Hexane Extracts ◽  
A Cell

Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in México and it lacks biotechnological studies for its use. The aim of this work was to establish a cell suspension cultures (CSC) of E. platycarpa, determine the phytochemical profile, and evaluate its antifungal activity. Friable callus and CSC were established with 2 mg/L 1-naphthaleneacetic acid plus 0.1 mg/L kinetin. The highest total phenolics of CSC was 15.6 mg GAE/g dry weight and the total flavonoids content ranged from 56.2 to 104.1 µg QE/g dry weight. CG‒MS analysis showed that the dichloromethane extracts of CSC, sapwood and heartwood have a high amount of hexadecanoic acid (22.3 ‒ 35.3 %) and steroids (13.5 ‒ 14.7%). Heartwood and sapwood defatted hexane extracts have the highest amount of stigmasterol (≈ 23.4%) and β-sitosterol (≈ 43%), and leaf extracts presented β-amyrin (16.3%). Methanolic leaves extracts showed mostly sugars and some polyols, mainly D-pinitol (74.3%). Dichloromethane and fatty hexane extracts of CSC exhibited the percentages inhibition higher for Sclerotium cepivorum with 71.5 and 62.0%, respectively. The maximum inhibition for Rhizoctonia solani was with fatty hexane extracts of the sapwood (51.4%). Our study suggest that CSC extracts could be used as a possible complementary alternative to synthetic fungicides.

scholarly journals Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of Extracts and Evaluation of Antifungal Activity

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 414
Author(s):  
Antonio Bernabé-Antonio ◽  
Alejandro Sánchez-Sánchez ◽  
Antonio Romero-Estrada ◽  
Juan Carlos Meza-Contreras ◽  
José Antonio Silva-Guzmán ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Dry Weight ◽  
Intact Plant ◽  
Leaf Extracts ◽  
Sclerotium Cepivorum ◽  
Hexane Extracts ◽  
A Cell

Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in Mexico. Biotechnological studies of its use are lacking. The objective of this work was to establish a cell suspension culture (CSC) of E. platycarpa, determine the phytochemical constituents by spectrophotometric and gas chromatography‒mass spectrometry (GC‒MS) methods, evaluate its antifungal activity, and compare them with the intact plant. Friable callus and CSC were established with 2 mg/L 1-naphthaleneacetic acid plus 0.1 mg/L kinetin. The highest total phenolics of CSC was 15.6 mg gallic acid equivalents (GAE)/g dry weight and the total flavonoids content ranged from 56.2 to 104.1 µg quercetin equivalents (QE)/g dry weight. The GC‒MS analysis showed that the dichloromethane extracts of CSC, sapwood, and heartwood have a high amount of hexadecanoic acid (22.3–35.3%) and steroids (13.5–14.7%). Heartwood and sapwood defatted hexane extracts have the highest amount of stigmasterol (~23.4%) and β-sitosterol (~43%), and leaf extracts presented β-amyrin (16.3%). Methanolic leaf extracts showed mostly sugars and some polyols, mainly D-pinitol (74.3%). Compared with the intact plant, dichloromethane and fatty hexane extracts of CSC exhibited percentages of inhibition higher for Sclerotium cepivorum: 71.5% and 62.0%, respectively. The maximum inhibition for Rhizoctonia solani was with fatty hexane extracts of the sapwood (51.4%). Our study suggests that CSC extracts could be used as a possible complementary alternative to synthetic fungicides.

Effects of different factors on friable callus induction and establishment of cell suspension culture of Hovenia dulcis (Rhamnaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Ivan Gonçalves Ribeiro ◽  
Tatiana Carvalho de Castro ◽  
Marsen Garcia Pinto Coelho ◽  
Norma Albarello
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Friable Callus ◽  
Weight Analysis ◽  
Hovenia Dulcis

Abstract Medicinal plants are an important therapeutic option for a large share of the world’s population. To establish an in vitro culture system for the production of secondary metabolites from Hovenia dulcis, we studied the effect of auxins, cytokinins, absence of light, and silver nitrate on the development of friable callus. Callus cultures were established for the first time and used to obtain cell suspension cultures. Supplementation with KIN (Kinetin) produced calli with both compact and friable areas, while the addition of TDZ (Thidiazuron) only produced compact callus. The maintenance of cultures in the dark induced a slight enhancement on friability when the auxin PIC (Picloram) was present in the culture medium. The addition of silver nitrate promoted the formation of friable calli. Dry weight analysis showed no significant differences in biomass growth, and, therefore, 2.0 mg.L-1 was considered the most suitable treatment. The presence of silver nitrate was not required for the establishment of cell suspension cultures. Dry weight analysis of cell suspensions showed higher biomass production in the absence of silver nitrate. PIC promoted 100% of cell suspension culture formation in the absence of silver nitrate, and higher biomass production was observed with the lowest concentration (0.625 mg.L-1). No morphological differences were observed among the different concentrations of PIC. Phytochemical screening showed the presence of saponins, flavonoids, flavonols and catechins in the extracts obtained from H. dulcis calli. These results show that the cell cultures herein established are potential sources for the production of H. dulcis secondary metabolites of medicinal interest.

Enhanced anthraquinone production in presence of silver nitrate in suspension culture of Gynochthodes umbellata (L.) Razafim. & B. Bremer (Rubiaceae), a traditional medicinal plant mentioned in Hortus Malabaricus

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Gangaprasad A
Keyword(s):  
Suspension Culture ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Fresh Weight ◽  
Friable Callus

Silver nitrate (AgNO3) enhanced production of anthraquinone was standardized in cell suspension cultures of Gynochthodes umbellata, a plant mentioned in the Hortus Malabaricus. The present research investigates the effect of silver nitrate, an abiotic elicitor on production of anthraquinone in in vitro cell suspension cultures of G. umbellata. Friable callus culture was established using in vitro derived leaf segment obtained from the nodal explant culture maintained in Murashige and Skoog (MS) medium containing 2 mg/l benzyl amino purine (BAP) and 3% sucrose. The in vitro derived leaf segments (0.5cm2) were cultured on MS medium containing 1 mg/l 2,4-D and 2% glucose for the production of friable callus. After 30 days of culture, uniform yellow friable callus was inoculated into MS liquid medium containing 1 mg/l 2,4-D and 2 % glucose for raising suspension culture. Uniform cell suspension was transferred to same media constituents and treated with different concentrations of AgNO3 on 25th day of culture. Fresh weight, dry weight and accumulation of anthraquinone content was studied and found that AgNO3 caused a marginal increase in biomass and anthraquinone based on the concentration and duration of AgNO3 treatment. A maximum fresh weight (19.48 g/fwt) dry weight (1.92g/dwt) and highest amount of anthraquinone content (48.62 mg/gdwt) were recorded in MS medium supplemented with 1 mg/l 2,4-D, 2%glucose and 3.5µM AgNO3 after 72 hrs of incubation.

scholarly journals Plant Regeneration from Cell Suspension Culture of Ulmus americana L.

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 461E-461
Author(s):  
Jing-Tian Ling ◽  
Roger J. Sauve
Keyword(s):  
Cell Suspension ◽  
Solid Medium ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Suspension Cells ◽  
Ulmus Americana ◽  
Friable Callus ◽  
Intact Plants ◽  
A Cell ◽  
Basal Salts

Leaf segments of greenhouse-grown Ulmus americana L. plants cultured on a Murashige and Skoog basal salts medium supplemented with 0.22 mg/L thidiazuron formed friable type of callus and regenerated shoots. This friable callus readily formed a cell suspension when the callus was placed in a liquid MS medium containing 2 mg/L 1-naphthaleneacetic acid and 1 mg/L 6-benzylaminopurine. Shoots were regenerated from 3-month-old suspension cell cultures after the suspension cells had been cultured on solid medium. Shoots developed roots on MS medium containing 0.1 mg/L indole-3-butyric acid. Intact plants were successfully established in soil.

scholarly journals INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

2017 ◽  
Vol 16 (1) ◽  
pp. 1-11
Author(s):  
Aryani Leksonowati ◽  
Witjaksono Witjaksono ◽  
Diah Ratnadewi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Formation ◽  
Combined Treatment ◽  
Cell Suspension Cultures ◽  
Compact Structure ◽  
Friable Callus ◽  
Aquilaria Malaccensis

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.

scholarly journals Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

2021 ◽  
Author(s):  
P.T.M. Tram ◽  
N.K. Suong ◽  
L.T.T. Tien
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Malignant Tumors ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Human Immune System ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

scholarly journals Pengaruh Sitokinin Eksogen dan Sukrosa terhadap Produksi Biomassa dan Alkaloid Canthinone di dalam Kultur Suspensi Sel Pasak Bumi (Eurycoma longifolia Jack.)

2012 ◽  
Vol 12 (2) ◽  
pp. 143
Author(s):  
Luthfi Aziz Mahmud Siregar
Keyword(s):  
Cell Suspension ◽  
Sucrose Concentration ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Eurycoma Longifolia ◽  
Eurycoma Longifolia Jack

The effect of addition cytokinins and modification of sucrose concentration on growth and alkaloid canthinoneproduction in cell suspension cultures of Eurycoma longifilia Jack were studied. The additions of cytokines, BAand kinetin, show effect on the production of biomass and alkaloid in cell suspension of E. longifilia Jack. Theoptimum totals of two-alkaloids were obtained on addition 4.44 μM BAP and without kinetin, respectively. Theaddition of 4.44 μM BA (6-benzyladenine) into TAM medium stimulated increased total of 9-hydroxycanthine-6-one,but decreased total of 9-methoxycanthin-6-one. While the addition of 2.32 - 9.29 μM kinetin (6-furfurylaminopurine)into TAM medium decreased total of two alkaloids (from 0.582 mg to 0.461 - 0.257 mg per 25 ml medium). Whensucrose concentration in TAM medium was increased from 3% to 5%, production of biomass would increase from0.374 g to 0.585 g dry weight per 25 ml medium. While total of two-alkaloids increase from 0.328 mg to 0.441 mgper 25 ml medium when concentration of sucrose in TAM medium was increased from 3% to 4% sucrose.

scholarly journals Antimicrobial activity of callus and cell suspension cultures extracts of Thevetia peruviana

2019 ◽  
Vol 8 (1) ◽  
pp. 45-56
Author(s):  
Juan Pablo Arias Echeverri ◽  
Isabel Cristina Ortega ◽  
Mariana Peñuela ◽  
Mario Arias
Keyword(s):  
Antimicrobial Activity ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Ornamental Plant ◽  
Thevetia Peruviana ◽  
Hexane Extracts ◽  
Ethanol Extracts ◽  
Different Parts

Thevetia peruviana is an ornamental plant considered source of biologically compounds with cardiac and antimicrobial activity. These compounds are normally extracted from different parts of the fully growth plants. In this work, extracts were obtained from callus and cell suspension cultures of T. peruviana and their antimicrobial activity was evaluated by disk diffusion tests against gram negative (Salmonella thipimurium and Escherichia coli) and gram positive (Staphylococcus aureus and Bacillus cereus) strains. Ethanol, methanol and hexane extracts from callus and cell suspension cultures showed biological activity. Methanolic cell suspension extract showed activity against B. cereus and S. aureus. Ethanolic cell suspension extract inhibit all the bacteria, especially S. thipimurium while hexanic extract showed resistance activity against S. thipimurium, S. aureus and B. cereus. In terms of the source of the extracts, hexane extracts obtained from cell suspension cultures showed a higher antimicrobial activity compared to callus, while ethanol extracts had an inverse behavior. These results outline in vitro cell culture of T. peruviana as a feasible biotechnological platform for the production of compounds with antimicrobial activity.

Phenolics from Cell Suspension Cultures of Penstemon serrulatus; Relation to Plant Organs

1997 ◽  
Vol 52 (7-8) ◽  
pp. 426-432 ◽  
Author(s):  
Zuzanna Skrzypek ◽  
Halina Wysokińska
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Naphthaleneacetic Acid ◽  
Phenolic Composition ◽  
Phenylpropanoid Glycosides ◽  
Selection Of

Abstract By repeated selection of pigment portions of tissue the red callus induced from root seed­lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/l), 6-benzylaminopurine (2 mg/l) and sucrose (50 g/l). From the cultured cells eight phenolic compounds were isolated. They were identified as cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, luteolin, luteolin 7-O-glucoside, norartocarpetin 7-O-glucoside, verbascoside, martynoside and leucosceptoside A. The kind of cell line, its age and light irradiation were important factors in flavonoid production, but production of phenylpropanoid glycosides was found to be unaffected by these factors. The phenolic composition found in the cell culture was compared with those in the flowers and leaves of original plants of P. serrulatus.

A Comparison of Various Growth Parameters of Cell Suspension Cultures to Determine Phytotoxicity of Xenobiotics

Weed Science ◽  
1984 ◽  
Vol 32 (2) ◽  
pp. 235-242 ◽  
Author(s):  
David G. Davis ◽  
Rosa L. Stolzenberg ◽  
Joan A. Dusky
Keyword(s):  
Ethylene Oxide ◽  
Cell Suspension ◽  
Culture Medium ◽  
Growth Parameters ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Weight Changes ◽  
Advantages And Disadvantages ◽  
Cell Volumes

An assessment was made of various parameters to measure growth of soybean [Glycine max (L.) Merr. ‘Wilkin’] and einkorn (Triticum monococcum L.) cell suspension cultures to establish convenient methods of screening the effects of chemicals. Methods assessed were settled cell volumes, packed cell volumes, absorbance at 525 nm of sonicated aliquots, dry weights (of aliquots or entire flask contents), and electrical conductivity and pH of the culture medium. Settled cell volumes, conductivity, and dry-weight changes were the most useful of the methods tested for determining the phytotoxicity of a nonionic linear alcohol ethylene oxide detergent (an adduct of 1-dodecanol containing eight ethylene oxide units) and the methyl ester of diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid}. Because 3 to 4 weeks were required to assess whether the cultures could grow out of the initial inhibition by the detergent or herbicide, none of the methods was rapid. Advantages and disadvantages of the various methods and their relative values for screening compounds are described.

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