The genetic structure of group A rotaviruses circulating in the Orenburg region in the 2013–2014 epidemic season

2016 ◽  
Vol 14 (1) ◽  
pp. 14-18
Author(s):  
N.B. Denisyuk ◽  
◽  
A.S. Pan’kov ◽  
Keyword(s):  
Genetic Structure ◽  
Epidemic Season ◽  
Orenburg Region ◽  
Group A Rotaviruses ◽  
Group A

scholarly journals Genetic characteristics of group A rotaviruses circulating in the Orenburg region during various epidemic seasons

2021 ◽  
Vol 13 (3) ◽  
pp. 82-91
Author(s):  
N. B. Denisyuk
Keyword(s):  
Genetic Structure ◽  
Central Research Institute ◽  
Molecular Diagnostic ◽  
Central Research ◽  
Entire Study ◽  
Genetic Characteristics ◽  
Orenburg Region ◽  
Leading Role ◽  
Group A Rotaviruses ◽  
Group A

Objective. To analyze the genetic structure of rotaviruses of group A, circulating on the territory of the Orenburg region in different epidemic seasons.Materials and methods: The genetic structure of rotaviruses by G/P alleles was determined in 341 fecal samples of patients with gastroenteritis clinic under the age of 5 years in various epidemic seasons. ELISA and PCR methods were used. The typing of rotaviruses was conducted on the basis of the laboratory of molecular diagnostic and epidemiology of enteric infections of the Department molecular diagnostics Central research Institute of epidemiology.Results: The general genetic structure of group a rotaviruses circulating in the Orenburg region in the period from 2014—2020 was determined, expressed, by the frequencies of G/P alleles. The genetic potential of group a rotaviruses for the entire study period was represented by twelve variants of genotypes for G/P alleles, the leading role in the epidemic process was played by genotypes with the highest frequency of occurrence: G4[P]8, G9[P]8, G2[P]4, Mixt. The frequency of registration of combined forms of various rotavirus genotypes (Mixed forms) was 5,9%.Conclusion: The main and dominant genotypes, their regional diversity and inter-seasonal fluctuations are established. The spectrum of circulating rotavirus genotypes differed in different epidemic seasons, and their epidemic significance is shown.

scholarly journals Rotaviruses and Rotavirus Vaccines

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 959
Author(s):  
Celeste M. Donato ◽  
Julie E. Bines
Keyword(s):  
Capsid Proteins ◽  
Virus Family ◽  
Rotavirus Vaccines ◽  
Group A Rotaviruses ◽  
Group A ◽  
Outer Capsid

Group A rotaviruses belong to the Reoviridae virus family and are classified into G and P genotypes based on the outer capsid proteins VP7 and VP4, respectively [...]

scholarly journals A Survey of G6 and G10 Serotypes of Group a Bovine Rotaviruses from Diarrheic Beef and Dairy Calves using Monoclonal Antibodies in ELISA

1994 ◽  
Vol 6 (2) ◽  
pp. 175-181 ◽  
Author(s):  
A. Lucchelli ◽  
S. Y. Kang ◽  
M. K. Jayasekera ◽  
A. V. Parwani ◽  
D. H. Zeman ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
South Dakota ◽  
Dairy Herd ◽  
Dairy Calves ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Stool ◽  
Field Samples ◽  
Group A Rotaviruses ◽  
Beef Herds ◽  
Group A

Group A bovine rotaviruses (BRV) have been identified worldwide as a major cause of diarrhea in the young of many species, including humans. Group A rotaviruses are classified into serotypes on the basis of the outer capsid proteins, VP7 (G types) and VP4 (P types). To date, there are 14 G types of group A rotaviruses, with G1, G6, G8, and G10 described for BRV isolates. In this study, G6- and G lo-specific monoclonal antibodies (MAbs) were used in an enzyme-linked immunosorbent assay (ELISA) for the G typing of BRV-positive stool samples from diarrheic beef and dairy calves from South Dakota, Ohio, Michigan, Nebraska, and Washington, USA, and Ontario, Canada. ELISA plates were coated using a broadly reactive VP7 MAb (Common 60) or with G6- or G10-specific MAbs. BRV-positive fecal samples were diluted and added to duplicate wells, followed by the addition of polyclonal guinea pig anti-group A rotavirus serum as the secondary antibody. Several reference G6 and G10 BRV strains as well as other G types previously reported in cattle (G1, G2, G3, G8) and BRV-negative samples were included as G type specificity and negative controls. From a total of 308 field samples analyzed, 79% (244/308) tested positive by the broadly reactive VP7 MAb; of these, 54% (131/244) were G6 positive, 14% (35/244) were G10 positive, 4% (9/244) were both G6 and G10 positive, and 28% (69/244) were G6 and G10 negative. The negative samples may represent additional or undefined serotypes. The 89 samples from South Dakota were further subdivided into samples from beef ( n = 43) or dairy ( n = 46) herds. G6 was more prevalent in beef herd samples (67%) than in dairy herd samples (47.5%). In addition, dairy herds had higher percentages of G10-positive samples (17.5%) G6-G10 double positives (10%), and untypable samples (25%) than did beef herds, in which the prevalence of G10 positive samples was 5.5%, G6-G10 double positives was 5.5%, and untypable samples was 22%. Application of the serotype ELISA for the analysis of additional BRV samples will provide further epidemiologic data on the distribution of BRV serotypes in beef or dairy cattle, an important consideration for the development of improved BRV vaccines.

scholarly journals Electropherotypes and G-Types of Group A Rotaviruses Detected in Children with Diarrhea in Lagos, Nigeria

ISRN Virology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Christianah Idowu Ayolabi ◽  
David Ajiboye Ojo ◽  
George Enyimah Armah
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Migration Pattern ◽  
Genomic Diversity ◽  
Rt Pcr ◽  
Group A Rotaviruses ◽  
Group A ◽  
Stool Samples ◽  
Health Care Centers ◽  
Virus Isolates

Approximately over 500,000 children die annually due to severe dehydrating diarrhea caused by rotaviruses. This work investigated rotavirus infection among children less than 5 years with diarrhea in Lagos and determined the circulating electropherotypes and genotypes of the virus isolates. Three hundred and two (n=302) stool samples from children below 60 months were collected from different hospitals and health care centers in Lagos and subjected to enzyme immunoassay (EIA) to determine the presence of Group A rotavirus, RT-PCR to determine the G-types, and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes. The results show that 60.3% of the samples showed distinct rotavirus RNA migration pattern, having long electropherotypes (55.3%) of seven variations dominating over the short electropherotypes (44.5%). Six different G-types were detected (G1, G2, G3, G4, G9, and G12). Serotypes G1 and G12 showed long electropherotypic pattern while G2, G3, and G9 exhibited either short or long electropherotype. All G4 detected show short electropherotypic pattern. In conclusion, information on the genomic diversity and RNA electropherotypes of rotaviruses detected in children with diarrhea in Lagos is reported in this study.

scholarly journals Survey of Human Group C Rotaviruses in Japan during the Winter of 1992 to 1993

1998 ◽  
Vol 36 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Mitsutaka Kuzuya ◽  
Ritsushi Fujii ◽  
Masako Hamano ◽  
Masao Yamada ◽  
Kuniko Shinozaki ◽  
...  
Keyword(s):  
Large Scale ◽  
Blot Hybridization ◽  
Human Group ◽  
Dot Blot ◽  
Passive Hemagglutination ◽  
Group A Rotaviruses ◽  
Group A ◽  
Outer Capsid ◽  
Very High ◽  
Vp7 Gene

Fecal specimens from patients with acute diarrhea were collected from 10 prefectures in Japan over a 6-month period (November 1992 to April 1993), and the specimens that were negative for human group A rotaviruses were screened for the presence of human group C rotaviruses (CHRVs) by the reverse passive hemagglutination test. Of 784 specimens examined, 53 samples (6.8%) that were collected in 7 of 10 prefectures were positive for CHRV, indicating that CHRVs are widely distributed across Japan. Most of the CHRV isolates were detected in March and April, and CHRVs mainly prevailed in children ages 3 to 8 years. The genome electropherotypes of eight strains isolated in five individual prefectures were surprisingly similar to each other and were different from those of CHRV strains isolated to date. The outer capsid glycoprotein (VP7) gene homologies of the isolates retrieved in 1993 were subsequently analyzed by the dot blot hybridization method. As a result, the VP7 genes of the isolates revealed very high levels of homology not only with each other but also with the VP7 gene of the OK118 strain isolated in 1988. These results suggest that a large-scale outbreak of CHRV occurred during the winter of 1992 and 1993 in Japan.

scholarly journals Suspected zoonotic transmission of rotavirus group A in Danish adults

2011 ◽  
Vol 140 (6) ◽  
pp. 1013-1017 ◽  
Author(s):  
S. E. MIDGLEY ◽  
C. K. HJULSAGER ◽  
L. E. LARSEN ◽  
G. FALKENHORST ◽  
B. BÖTTIGER
Keyword(s):  
Genetic Relatedness ◽  
Phylogenetic Analyses ◽  
Geographical Area ◽  
Nucleotide Sequences ◽  
Zoonotic Transmission ◽  
Adult Patients ◽  
Epidemiological Features ◽  
Group A Rotaviruses ◽  
Group A ◽  
Stool Samples

SUMMARYGroup A rotaviruses infect humans and a variety of animals. In July 2006 a rare rotavirus strain with G8P[14] specificity was identified in the stool samples of two adult patients with diarrheoa, who lived in the same geographical area in Denmark. Nucleotide sequences of the VP7, VP4, VP6, and NSP4 genes of the identified strains were identical. Phylogenetic analyses showed that both Danish G8P[14] strains clustered with rotaviruses of animal, mainly, bovine and caprine, origin. The high genetic relatedness to animal rotaviruses and the atypical epidemiological features suggest that these human G8P[14] strains were acquired through direct zoonotic transmission events.

scholarly journals Isolation and identification of group A rotaviruses among neonatal diarrheic calves, Morocco

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Imane Ennima ◽  
Ghizlane Sebbar ◽  
Bachir Harif ◽  
Saaid Amzazi ◽  
Chafiqa Loutfi ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Group A Rotaviruses ◽  
Group A ◽  
Diarrheic Calves

scholarly journals Molecular Characterizations of Human and Animal Group A Rotaviruses in The Netherlands

2005 ◽  
Vol 43 (2) ◽  
pp. 669-675 ◽  
Author(s):  
R. van der Heide ◽  
M. P. G. Koopmans ◽  
N. Shekary ◽  
D. J. Houwers ◽  
Y. T. H. P. van Duynhoven ◽  
...  
Keyword(s):  
The Netherlands ◽  
Animal Group ◽  
Group A Rotaviruses ◽  
Group A

Molecular characterization of the first human G15 rotavirus strain of zoonotic origin from the bovine species

2021 ◽  
Vol 102 (4) ◽  
Author(s):  
Takeshi Tsugawa ◽  
Yoshiki Fujii ◽  
Yusuke Akane ◽  
Saho Honjo ◽  
Kenji Kondo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Human Infection ◽  
Potential Reservoir ◽  
Group A Rotaviruses ◽  
Paediatric Outpatient ◽  
Group A ◽  
Human Infections ◽  
Bovine Species

Group A rotaviruses (RVAs) infect a wide variety of mammalian and avian species. Animals act as a potential reservoir to RVA human infections by direct virion transmission or by contributing genes to reassortants. Here, we report the molecular characterization of a rare human RVA strain Ni17-46 with a genotype G15P[14], isolated in Japan in 2017 during rotavirus surveillance in a paediatric outpatient clinic. The genome constellation of this strain was G15-P[14]-I2-R2-C2-M2-A13-N2-T9-E2-H3. This is the first report of an RVA with G15 genotype in humans, and sequencing and phylogenetic analysis results suggest that human infection with this strain has zoonotic origin from the bovine species. Given the fact that this strain was isolated from a patient with gastroenteritis and dehydration symptoms, we must take into account the virulence of this strain in humans.

Sign in / Sign up

Export Citation Format

Share Document