scholarly journals Growth of Candida sp and Aspergillus sp from Bronchoscopy Pulmonary Tuberculosis Patients on Bran Media

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Mujahidah Basarang ◽  
Muhammad Rifo Rianto
Keyword(s):  
Pulmonary Tuberculosis ◽  
Fungal Growth ◽  
Raw Material ◽  
Growth Media ◽  
Carbohydrate Source ◽  
Tuberculosis Patients ◽  
Culture Techniques ◽  
Candida Sp ◽  
Untreated Culture ◽  
Aspergillus Sp

Pulmonary tuberculosis caused by Mycobacterium tuberculosis become ahealth problem in Indonesia. The chronic nature of this disease is further exacerbated ifit is accompanied by fungal infection such as Candida albicans and Aspergillus sp.,which is usually remains undiagnosed and thus untreated. Culture techniques can beused to identify Candida sp and Aspergillus sp from bronchoscopy. Fungal culturemedia in laboratory containing high carbohydrate source, nitrogen source are requiredfor the growth. This nutrient can be found in bran that contains high carbohydrates,proteins, fats, vitamins, and crude fiber. So that bran can be used as raw material for alternative fungal growth media. The purpose of this study was to increase bran as amedium for the growth of Candida sp and Aspergillus sp isolated from bronchoscopy ofpulmonary TB patients. This study included bran collection, preparation of bran media,inoculation bronchoscopy on bran media, observation of fungal growth. Colonies ofCandida sp and Aspergillus sp were confirmed microscopically. The results showed thatCandida sp and Aspergillus sp grew on both media, Bekatul Dextrose Agar and PotatoDextrose Agar. The conclusion of this study is that bran can be used as a medium forfungal growth. Bran media can be used as an alternative media to replace syntheticmedia to grow Candida sp and Aspergillus sp isolated from bronchial rinses.

scholarly journals Fungi Identification in Preservative Liquids of Cadaver at Anatomy Laboratory of Faculty of Medicine Maranatha Christian University Bandung

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Mochamad H. Herdiman ◽  
Triswaty Winata ◽  
Tediany Pramesti ◽  
Imam G. Alam
Keyword(s):  
Fungal Growth ◽  
Microbiology Laboratory ◽  
Christian University ◽  
Candida Sp ◽  
Penicillium Sp ◽  
Anatomy Laboratory ◽  
Aspergillus Sp

Cadaver is an important learning media for students of the Faculty of Medicine. Althoughthe cadaver has undergone a preservation process, sometimes the preservative liquids used onlyprotects the cadaver, and does not inhibit the fungal growth. Based on previous research, thefungus found in cadaveric preparations are pathogenic. The objectives of this study were toidentify the growing fungus on the cadaver preservative liquid at the Anatomy Laboratory of theFaculty of Medicine, Maranatha Christian University. This is a descriptive experimental study.Samples were taken from three cadaver tubs. Subsequently, the sample was inoculated onSabouraud's Dextrose Agar and Chromagar medium in a petridish and incubated at roomtemperature (25 ⁰ ) for seven days. After seven days, the morphology of the fungus was observedmacroscopically and microscopically. We found Aspergillus sp., Penicillium sp.,Epidermophyton sp., and Candida sp on the samples. The study was conducted in December2017-January 2018 at the Microbiology Laboratory, Faculty of Medicine, Maranatha ChristianUniversity and Microbiology Laboratory, Faculty of Medicine, Padjadjaran University.Keywords: fungi, cadaver, preservative liquid

scholarly journals Tuberculosis-Candida Co-Infection in Patients having Pulmonary Tuberculosis Attending DOTs Clinic in Rumuigbo Model Primary Health Centre in Port Harcourt, Nigeria

2019 ◽  
pp. 1-7
Author(s):  
Asikiya Hanson ◽  
Amala Smart Enoch ◽  
Gloria N. Wokem
Keyword(s):  
Pulmonary Tuberculosis ◽  
Health Centre ◽  
Staining Technique ◽  
Tuberculosis Patients ◽  
Candida Sp ◽  
Tube Test ◽  
Predominant Species ◽  
Sugar Fermentation ◽  
Primary Health ◽  
Port Harcourt

Introduction: Some pulmonary tuberculosis subjects were some co-infected with Candida such as C. albicans, C. tropicalis, C. kruezi and C. paraspsilosis which were initially thought to be normal floras of the oral cavity. The percentage of tuberculosis patients co-infected with Candida is becoming a concern and might complicate the treatment of tuberculosis. Materials and Methods: A total of 400 sputum samples were collected and subjected to Ziehl-Neelsen staining technique and Genexpert system, Gram’s stain, Germ tube test and examination in KOH preparation were conducted. Culture on Sabouraud Dextrose Agar with gentamicin, and cultured on CHROMagar Candida and sugar fermentation were carried out for Candida. Results: Out of the 400 sputum samples examined 93(23.3%) had TB and 32(8.0%) were positive for Candida. By gender the prevalence of tuberculosis were females 51(22.4%), males 42(24.4%) while the prevalence of Candida were females 18(35.3%) and males 14(33.3%). The percentage occurrences of Candida sp. isolated were C. albicans which was the predominant species 10(21.5%), C. tropicals 5(5.4%), C. krusei 4(4.3%) and C. Parapsilosis 3(3.2%) respectively. Conclusion: Candida albicans remains the most predominant species of Candida in patients suffering from pulmonary tuberculosis and colonization by Candida sp should not be ignored. The presence of Candida sp. might contribute to in one way to the progression of the disease.

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Kualitas Hidup Pasien Tuberkulosis Paru yang Mengalami Stigma Diri (Quality of Life Pulmonary Tuberculosis Patients With Self Stigma))

2019 ◽  
Vol 3 (2) ◽  
pp. 17-27
Author(s):  
Yunita Sari
Keyword(s):  
Quality Of Life ◽  
Pulmonary Tuberculosis ◽  
Descriptive Study ◽  
Negative Influence ◽  
Questionnaire Data ◽  
Average Quality ◽  
Tuberculosis Patients ◽  
Pulmonary Tb ◽  
Life Score

Pulmonary tuberculosis (TB) is a chronic disease that can bring about the sufferer's self-stigma and also affect his quality of life. A number of studies report that living with TB has a negative influence on the quality of life of sufferers even with or without self-stigma. The purpose of this study was to identify the quality of life of TB patients who experienced self-stigma. This research is a descriptive study, sample were 31 pulmonary TB patients. Data was collected using a questionnaire. Data analyzed by using frequency distribution and percentage. The researcher first screened TB patients who experienced self-stigma. The results showed that 25 people (80.64%) respondents experienced mild self-stigma. A total of 9 respondents (36%) had a quality of life score in the good category and as many as 16 respondents (64%) had enough category with an average quality of life score is 56.57. While respondents who had moderate self-stigma were 6 people (19.36%) with a good quality of life score was 1 person (16.67%) and enough category quality of life score were 5 people (83.33%) with an average quality of life score is 49.92.

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

Sign in / Sign up

Export Citation Format

Share Document