DETERMINATION OF TOTAL FLAVONOID CONTENT IN ETHANOLIC LEAF EXTRACT OF Moringa Oleifera

2017 ◽  
pp. 849-852
Author(s):  
Shanmugapriya S.
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ethanolic Leaf Extract

scholarly journals Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

2021 ◽  
Vol 8 (3) ◽  
pp. 1-4
Author(s):  
Nurlinda Nurlinda ◽  
Virsa Handayani ◽  
Faradiba Abdul Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Caesalpinia Sappan ◽  
Antimicrobial Treatments

AbstractBiancaea sappan  (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

scholarly journals Microscopic Identification and Determination of Total Flavonoid Content of Moringa Leaves Extract and Ethyl Acetate Fraction (Moringa oleifera L.)

2022 ◽  
Vol 1 ◽  
pp. 66
Author(s):  
Annisa Fatmawati ◽  
Depita Sucianingsih ◽  
Riza Kurniawati ◽  
Muhammad Abdurrahman
Keyword(s):  
Ethyl Acetate ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Microscopic Identification

This research was conducted to identify simplicia microscopically, phytochemical screening and determination of total flavonoid content of extract and ethyl acetate fraction from Moringa (Moringa oleifera L.) leaves using UV-Visible Spectrophotometry method. The experimental design used in this study was to perform microscopic identification of Moringa leaf powder simplicia, make 96% and 70% ethanol extract and ethyl acetate fraction of Moringa leaves from 70% ethanol extract, then carry out phytochemical screening and determination of total flavonoid content with quercetin standards. Phytochemical screening on the ethyl acetate fraction of Moringa leaves included tests for the content of flavonoids, saponins, tannins and alkaloids. The results of microscopic identification of Moringa leaf simplicia showed the presence of calcium oxalate crystals in the form of rosettes, mesophyll and stomata. The result of determination of total flavonoid content in 96% ethanol extract was 16.69 ± 0.74% (w/w), 70% ethanol extract was 10.84 ± 0.49% (w/w), Moringa leaf ethyl acetate fraction 14 .45 ± 0.90% (w/w). The highest total flavonoid content was found in the 96% ethanol extract of Moringa leaves in accordance with the 2017 Indonesian Herbal Pharmacopoeia, that the thick extract of Moringa leaves containing no less than 6.30% (w/w) total flavonoids was calculated as quercetin.

Study on Changes of Total Flavonoid Content in Suaeda salsa in Different Months of Growing Season

2014 ◽  
Vol 926-930 ◽  
pp. 1028-1031
Author(s):  
Wei Fang Zhong ◽  
Yue Hong Wang ◽  
Hong Ying Liu ◽  
Jin Hua Feng
Keyword(s):  
Theoretical Basis ◽  
Growing Season ◽  
Total Flavonoid ◽  
Suaeda Salsa ◽  
Uv Spectrophotometry ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Significant Difference

Objective To investigate the changes of total flavonoid content of Suaeda salsa in different months of growing season, from May to November. Methods UV spectrophotometry was used for determination of the total flavonoid. Results The content of total flavonoid in Suaeda salsa was highest in September (67.75 mg·g-1) and was lowest in May (11.4 mg·g-1).The content was found to increase from May to September, and to decrease from September to November. Conclusion There is significant difference between total flavonoid content in Suaeda salsa from different months, which can be used to provide theoretical basis for harvesting and processing of Suaeda salsa.

scholarly journals DETERMINATION OF THE QUALITY CHARACTERISTICS OF NATURALLY GROWING HAWTHORN IN SUŞEHRI

2020 ◽  
Vol 19 (1) ◽  
pp. 61-70
Author(s):  
Erdal Ağlar ◽  
Ahmet Sümbül ◽  
Orhan Karakaya ◽  
Burhan Ozturk
Keyword(s):  
Antioxidant Activity ◽  
Fruit Weight ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Fruit Characteristics ◽  
Ph Values ◽  
Biochemical Measurements

The study was conducted in 2017 in the district of Sivas. In the study, 20 genotypes, which are considered to be different from each other, taking into account the fruit characteristics such as color and size and shape, were determined from the hawthorns that were naturally grown in the flora of Suşheri. At harvest time, the fruit, which would be adequate for pomological and biochemical measurements and analyzes, was harvested. According to the results of the measurements and analyzes in the study, fruit weight was found to vary between 0.68 g and 6.35 g, fruit width was between 10.52 and 29.48 mm and fruit length was between 11.40 and 20.67 mm. The highest firmness values were recorded with the genotype (G) 20 genotype, while the G4 had the lowest values in terms of the firmness values of the fruit flesh. While there are no significant differences between the pH values of the genotypes, the differences between the genotypes in terms of SSC, TA and vitamin C contents are quite significant. It has been found that there are significant differences between the genotypes in terms of total phenolic, total flavonoid and antioxidant activity. The total phenolic content ranged from 218.8 (G17) to 605.8 (G5 and G8) mg GAE kg–1 f.w., while the lowest total flavonoid content was 21.58 (G 17) and the highest total flavonoid content was 67.75 (G9) mg GAE kg–1 f.w. When the antioxidant activity was evaluated, the DPPH values were 1.08 (G17) – 15.43 (14) mmol TE kg–1 f.w., the FRAP values were 15.43 (G16) – 47.23 (G8) mmol TE kg–1 f.w. respectively.

scholarly journals EFFECT OF METHANOL CONCENTRATION AS A SOLVENT ON TOTAL PHENOLIC AND FLAVONOID CONTENT OF BELUNTAS LEAF EXTRACT (Pulchea indica L.)

2020 ◽  
Vol 2 (2) ◽  
pp. 77
Author(s):  
Ni Putu Sinta Mahasuari ◽  
N. L. P. Vidya Paramita ◽  
A.A Gede Rai Yadnya Putra
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Total Phenol ◽  
Methanol Concentration ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Caffeoylquinic Acid ◽  
Phenolic And Flavonoid

Background: Beluntas (Pluchea indica L.) is an Indonesian plant that grows wild and is used as traditional medicine. Beluntas leaves are reported to contain phenolic, and flavonoid is a part of phenolic compounds. Phenolic compounds other than flavonoids include 1,3,4,5-tetra-O-caffeoylquinic acid, 3,4,5-tri-O-caffeoylquinic acid, chlorogenic acid, and ferulic acid. Flavonoid compounds in beluntas leaves are quercetin, apigenin, luteolin and chrysoeriol. Methanol solvents are reported to be able to extract higher polyphenol and flavonoid contents than other solvents. Objective: The purpose of this study is to determine the effect of 20%, 50% and 75% solvent concentration of methanol on total phenol and total flavonoid levels of beluntas leaf extract. Methods: In this study, the extraction process was carried out by maceration, the determination of total phenol content was carried out by the Follin-Ciocalteu method. Determination of total flavonoid levels was carried out by the Colorimetric method. Data on phenol and flavonoid levels were analyzed statistically. Results: The yield of beluntas leaf extract in this study was respectively from the lowest methanol concentration of 24.094% w/w, 31.126% w/w, 24.838% w/w. The value of total phenol levels increased with increasing methanol concentration, namely 124.84 mg GAE/g, 138.3 mg GAE/g, and 147.91 mg GAE/g. The highest total flavonoid value in 75% methanol extract is 69.72 mg QE/g, followed by 20% methanol extract at 46.29 mg QE/g and the lowest is found in 50% methanol extract at 32.80 mg QE/g. The results of statistical analysis using the Kruskal-Wallis test showed that there were significant differences (p<0.05) in the value of total phenol levels and total flavonoids of the three extracts. Conclusion: The difference in the concentration of methanol solvents affects the value of total phenol levels and total flavonoids with the highest value produced by a 75% methanol solvent. Keywords: Beluntas Leaves (Pluchea indica L.), Total Phenol Content, Total Flavonoid Content, Gallic Acid, Quercetin.

scholarly journals Determination of Biological Activities of the Root Bark of Senna singueana

2020 ◽  
pp. 25-34
Author(s):  
Kidanemariam Teklay Hilawea ◽  
Zelalem Yibralign Desta
Keyword(s):  
Antibacterial Activity ◽  
Biological Activities ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aims: Senna singueana is a medicinal plant which is used for the treatment of different kinds of diseases and the plant was selected for the study because of its numerous uses. The main objective of this study was to determine the antioxidant and antibacterial activity of the extracts of the root barks of S. singueana. Methodology: The antibacterial activities of the extracts (determination of Minimal Inhibitory “MIC” and Minimal Bactericidal Concentration “MBC”) were determined by using agar well diffusion method. In addition to this the total flavonoid and total phenolic contents were determined by using aluminum chloride colorimetric complex assay and Folin-Ciocalteu method respectively. Results: Our results revealed that the total flavonoid content of the extracts is ranged from 30.39 mgQE/100 g to 240.83 mgQE/100 g. The extracts also showed good antioxidant activity and total phenolic content as well as weak to moderate antibacterial activity against some bacteria. Conclusions: The extracts of the root bark of Senna sinueana showed good total flavonoid content, DPPH radical scavenging activity and antibacterial activity. In addition to this, the extracts also showed the presence of some important compounds by phytochemical analysis.

scholarly journals Drumstick (Moringa oleifera) variation in biomass and total flavonoid content in Indonesia

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ridwan RIDWAN ◽  
Hamim Hamim ◽  
Suharsono SUHARSONO ◽  
Nuril Hidayati ◽  
INDRA GUNAWAN
Keyword(s):  
Antioxidant Activity ◽  
Biomass Production ◽  
Moringa Oleifera ◽  
Block Design ◽  
Total Flavonoid ◽  
Leaf Biomass ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Tropical Country ◽  
Superior Genotype

Abstract. Ridwan, Hamim, Suharsono, Hidayati N, Gunawan I. 2021. Drumstick (Moringa oleifera) variation in biomass and total flavonoid content in Indonesia. Biodiversitas 22: 493-500. Utilization of a drumstick (Moringa oleifera Lam.) leaf has recently increased worldwide which consequently cause the increase in global demand. Indonesia, as a tropical country with large area, has a great potential to become a center for drumstick cultivation and leaf production. Drumstick distribution scattered in almost all of the islands of Indonesia has another potential for obtaining superior genotype variabilities. The aim of this study was to select the drumstick accessions from several islands in Indonesia with high leaf biomass production and flavonoid content. This experiment was carried out in the field using ten accessions of drumstick from ten islands in Indonesia, namely Sumatra, Java, Madura, Bali, Lombok, Sumbawa, Sumba, Kalimantan, Sulawesi, and Papua. Drumstick seeds were planted in polybags with a capacity of 10 kg and arranged using a randomized block design with three replications. The parameters observed were plant growth, biomass production, total flavonoid content, and antioxidant activity. The results of this study found that Sumatra accession was the accession which had the highest leaf biomass production in combination with the uppermost total flavonoid content and antioxidant activity compared to others. Sumatra accession is recommended as an excellent accession for cultivation with the aim of producing flavonoids.

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