Antibacterial Activity of Germacrone Sesquiterpene from Curcuma Xanthorrhiza Rhizomes

Hartiwi Diastuti; Yana Maolana Syah; Lia Dewi Juliawaty; Marlia Singgih

doi:10.20961/alchemy.12.2.1726.103-111

Antibacterial Activity of Germacrone Sesquiterpene from Curcuma Xanthorrhiza Rhizomes

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v12i2.1726 ◽

2016 ◽

Vol 12 (2) ◽

pp. 103 ◽

Cited By ~ 1

Author(s):

Hartiwi Diastuti ◽

Yana Maolana Syah ◽

Lia Dewi Juliawaty ◽

Marlia Singgih

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Enterobacter Aerogenes ◽

Salmonella Typhi ◽

White Crystal ◽

H Nmr ◽

Curcuma Xanthorrhiza ◽

Vacuum Liquid Chromatography ◽

C Nmr

The aim of this research was to isolate and indentify the terpenoid compound from Curcuma xanthorrhiza rhizomes and its antibacterial activity. Isolation was carried out by using vacuum liquid chromatography and centrifugal chromatography. The structure was determined by NMR spectroscopy (1H-NMR, 13C-NMR 1D and 2D), then compare with data from literatures. Antibacterial test was carried out by using microdillution methods and evaluated against eight bacteria. They are Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae and Vibrio cholerae. The result showed that the isolate was a white crystal which was indetified as germacron-type sesquiterpene. Germacron have highest activity againts P. aeruginosa, MIC 15.6 µg/mL and MBC 31.2 µg/mL.

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Composition and Antibacterial Activity of Essential Oils from Leaf, Stem and Root of Chrysanthemum parthenium (L.) Bernh. from Iran

Natural Product Communications ◽

10.1177/1934578x0900400624 ◽

2009 ◽

Vol 4 (6) ◽

pp. 1934578X0900400 ◽

Cited By ~ 4

Author(s):

Ali Shafaghat ◽

Hajar Sadeghi ◽

Khodamali Oji

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oils ◽

Salmonella Typhi ◽

Inhibitory Effects ◽

Germacrene D ◽

Leaf Oil ◽

Main Components ◽

Microdilution Broth

The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-β-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were α-pinene (50.0%), trans-β-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the microdilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.

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Complexations of 2-thiouracil and 2,4-dithiouracil with Cd(SeCN)2and Hg(SeCN)2: NMR and anti-bacterial activity studies

Spectroscopy An International Journal ◽

10.1155/2011/586046 ◽

2011 ◽

Vol 25 (3-4) ◽

pp. 187-195 ◽

Cited By ~ 1

Author(s):

M. Nasiruzzaman Shaikh ◽

Bassem A. Al-Maythalony ◽

Mohammed I. M. Wazeer ◽

Anvarhusein A. Isab

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Solid State ◽

Salmonella Typhi ◽

Bacterial Activity ◽

Spectroscopic Techniques ◽

Standard Type ◽

Nmr Data ◽

C Nmr

Cadmium and mercury selenocyanate complexes of 2-thiouracil (TU) and 2,4-dithiouracil (DTU) ligands have been synthesized to form complexes of the type [M(SeCN)2(TU)] and [M(SeCN)2(DTU)] (where M is Cd2+or Hg2+) and studied by various spectroscopic techniques such as IR,1H and13C NMR in solution and in the solid state for13C,15N, and113Cd nuclei. Based on IR, and solution and solid-state13C NMR data, stronger cadmium bonding to the thiouracil was observed compared to that of mercury. Anti-bacterial activities of these complexes have been investigated with standard type culture ofEscherichia coli(MTCC 443),Klebsiella pneumoniae(MTCC 109),Pseudomonas aeruginosa(MTCC 1688),Salmonella typhi(MTCC 733) andStaphylococcus aureus(MTCC 737) and show that ligands exhibit more anti-bacterial activities than that of the corresponding Cd(II) and Hg(II) complexes.

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Antibacterial Activity of Leaves and Rhizome of Curcuma xanthorrhiza Essential Oils on Different Distillation Time

Jurnal Jamu Indonesia ◽

10.29244/jji.v3i3.69 ◽

2018 ◽

Vol 3 (3) ◽

pp. 116-120

Author(s):

Nur Syafiqah Nadiah Mohammad Rafi ◽

Irmanida Batubara ◽

Anthony Nyangson Steven

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oils ◽

Mobile Phase ◽

Clear Zone ◽

Rf Values ◽

Curcuma Xanthorrhiza ◽

Layer Chromatography ◽

And Staphylococcus Aureus

This research aims to determine the antibacterial activity of Curcuma xanthorrhiza leaves and rhizomes essential oil with different distillation time of 2 hours, 4 hours and 6 hours against Escherichia coli and Staphylococcus aureus. The essential oils produced then separated by thin layer chromatography (TLC) with n-hexane: toluene: dichloromethane 1:8:1 as the mobile phase. The result shows that on the first two hours distillation gave the highest yield compared to the next two hours or four hours. Based on TLC chromatograms, the highest content of compounds found at 4-6 hours distillation time. TLC bioautography contact was used for antibacterial activity tested on chromatogram and the clear zone appears on it indicates the spots were active as antibacterial activity against both bacteria with Rf values of 0.35 and 0.49 for rhizomes, rhizome essential oils 4-6 hours distillation time as the most significance active.

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TRANSFORMATION OF ETHYL-P-METHOXYCINNAMATE TO P –METHOXYCINNAMIC ACID FROM KENCUR (Kaempheria galanga L.) AND THEIR ANTIBACTERIAL ACTIVITY

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.13.2.8472.176-190 ◽

2017 ◽

Vol 13 (2) ◽

pp. 176

Author(s):

Muhamad Salman Fareza ◽

Rehana Rehana ◽

Nuryanti Nuryanti ◽

Didin Mujahidin

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Separation And Purification ◽

E Coli ◽

H Nmr ◽

Alkaline Conditions ◽

Methoxycinnamic Acid ◽

Vacuum Liquid Chromatography ◽

Hydrolysis Of ◽

C Nmr

This study aimed to evaluate the antibacterial properties of ethyl-p-methoxycinnamate and p-methoxycinnamate acid from Kaempheria galanga L. Ethyl-p-methoxycinnamate was isolated from the n-hexane rhizome extract of Kaempheria galanga L. Separation and purification of this compound was carried out with vacuum liquid chromatography and column chromatography. Hydrolysis of ethyl-p-methoxycinnamic under alkaline conditions obtained p-methoxycinnamic acid with a good yield of 85 %. The structure of the compounds were charactrized with IR, NMR spectrophotometer (1H-NMR and 13C-NMR) and mass spectrophotometer. The antibacterial properties of the compounds were evaluated using microdilution methods against B. cereus ATCC 11778, L. monocytogenes ATCC 7644, E. coli ATCC 25922, S. enterica sv Typhimurium ATCC 14028, and E. aerogenes ATCC 13048. The compounds showed weak antibacterial properties. Only ethyl p-methoxycinnamate showed the strongest antibacterial activity, especially against B. cereus ATCC 11778 bacteria with MIC values of 62.5 mg /mL. The change of the functional groups provided no significant impact on the antibacterial activity.

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Sophoraflavanone G from Sophora pachycarpa Enhanced the Antibacterial Activity of Gentamycin against Staphylococcus aureus

Zeitschrift für Naturforschung C ◽

10.1515/znc-2006-9-1026 ◽

2006 ◽

Vol 61 (9-10) ◽

pp. 769-772 ◽

Cited By ~ 14

Author(s):

Ali Fakhimi ◽

Mehrdad Iranshahi ◽

Seyed Ahmad Emami ◽

Esam Amin-Ar-Ramimeh ◽

Gholamreza Zarrini ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Plant Extract ◽

Acetone Extract ◽

Enhancement Effect ◽

Disc Diffusion ◽

H Nmr ◽

Broth Dilution ◽

Mic Value ◽

C Nmr

AbstractIn this study the enhancement effect of Sophora pachycarpa roots’ acetone extract on the antibacterial activity of gentamycin was evaluated against Staphylococcus aureus. Disc diffusion and broth dilution methods were used to determine the antibacterial activity of gentamycin in the absence and presence of plant extract and its various fractions separated by TLC. A clinical isolate of S. aureus was used as test strain. The active component of the plant extract involved in enhancement of gentamycin’s activity had Rƒ = 0.72 on a TLC plate. The spectral data (1H NMR, 13C NMR) of this compound revealed that this compound was 5,7,2′,4′-tetrahydroxy-8- lavandulylflavanone (sophoraflavanone G), previously isolated from Sophora exigua. In the presence of 0.03 μg/ mL of sophoraflavanone G the MIC value of gentamycin for S. aureus decreased from 32 to 8 μg/mL (a fourfold decrease). These results signify that the ultra-low concentration of sophoraflavanone G potentiates the antimicrobial action of gentamycin suggesting a possible utilization of this compound in combination therapy against Staphylococcus aureus.

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Synthesis and Reactions of 3-(2-Chloromethyl-carbonylamino)tetrachloroquinazolin-2,4-dione

Organic Chemistry International ◽

10.1155/2012/284947 ◽

2012 ◽

Vol 2012 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

M. A. Hassan ◽

A. M. M. Younes ◽

M. M. Taha ◽

A. Abdel-Monsef

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Elemental Analysis ◽

Salmonella Typhi ◽

Synthetic Route ◽

H Nmr ◽

In Vitro Antibacterial Activity

A series of tetrachloroquinazolin-2,4-dione derivatives were synthesized using appropriate synthetic route and characterized by IR, 1H NMR, MS, and elemental analysis. The synthesized compounds were evaluated for their preliminary in vitro antibacterial activity towards Salmonella typhi, Staphylococcus aureus, and Bacillus cereus.

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Antibacterial and Phytochemical Properties of Crude Leaf Extracts of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on Some Clinical Bacterial Isolates in Nigeria

Journal of Complementary and Alternative Medical Research ◽

10.9734/jocamr/2020/v10i430168 ◽

2020 ◽

pp. 1-15

Author(s):

Reginald C. Njokuocha ◽

Anthonia E. Ewenike

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Salmonella Typhi ◽

Bacterial Isolates ◽

Leaf Extracts ◽

Ceiba Pentandra ◽

Ethanolic Extracts ◽

Pterocarpus Santalinoides

Aims: The study was carried out to determine the phytochemical constituents and antibacterial activity of aqueous and ethanolic extracts of fresh leaves of Moringa oleifera Lam., Pterocarpus santalinoides L’Herit DC and Ceiba pentandra L. on bacterial isolates; Salmonella typhi, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Methodology: The plant leaves were dried, pulverized and phytochemical tests were done according to standard laboratory procedure. Aqueous and ethanolic extracts were obtained from 20 g of the of the ground leaves. Antibacterial assay was carried out with Disc diffusion method on seven concentrations of the extracts ;100,50,25,12.5, 6.25,3.125,1.5625 mg/ml and compared with standard antibiotics. Isolated bacterial pathogens; Salmonella typhi, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa (1.0 x 105 cfu /ml) were used as test organisms. Results: Alkaloids, steroidal aglycones, glycosides, proteins, carbohydrates, reducing sugars, tannins, saponins, vitamins A and E were present in all the plant samples. Flavonoids and cardiac glycosides were not detected in Pterocarpus santalinoides and Ceiba pentandra, respectively. Anthracene glycoside was absent in all samples. Aqueous and ethanolic extracts of M. oleifera showed antibacterial activities against all the bacterial isolates at minimum inhibitory concentration (MIC) of 3.125 mg/ml and 1.5625 mg/ml respectively. Pterocarpus santalinoides showed inhibitory activity only on Salmonella typhi at 3.125 mg/ml and Escherichia coli 1.5625 mg/ml MIC. Ceiba pentandra showed spectrum of antibacterial activity against all the bacterial isolates at 1.56 mg/ml MIC with exception of Salmonella typhi. E. coli was the most susceptible to the leaf extracts. Salmonella typhi was not sensitive to the leaf extracts of Ceiba pentandra, while Staphylococcus aureus and Pseudomonas aeruginosa were not sensitive to the leaf extracts of Pterocarpus santalinoides. Conclusion: It can be concluded that both aqueous and ethanolic leaf extracts had antibacterial activity against the test organism, thus justifying their use in folklore medicine.

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Synthesis and Antimicrobial Activity of Some [1,2,4]-Triazole Derivatives

Journal of Chemistry ◽

10.1155/2013/462594 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Bhimagouda S. Patil ◽

G. Krishnamurthy ◽

N. D. Shashikumar ◽

M. R. Lokesh ◽

H. S. Bhojya Naik

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Triazole Derivatives ◽

H Nmr ◽

Chemical Structures ◽

C Nmr

A series of novel [1,2,4]-triazolo piperidine (8), [1,2,4]-triazolo piperazine (9a-c), [1,2,4]-triazolo phenylether (10a-e), and [1,2,4]-triazolo aniline (11a-c) derivatives have been synthesized. The chemical structures of the newly synthesized compounds were characterized by IR,1H NMR,13C NMR, and LCMS. The newly synthesized compounds were screened for antimicrobial activity. Among all the compounds tested,11b(R4=4-MeO–) showed the highest activity againstStaphylococcus aureusandEscherichia coli, and9a(R1andR2=Cl) showed the highest activity againstPseudomonas aeruginosa.

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Ferrocene-Based Antibacterial Agents: Synthesis, Characterization, and Biological Evaluation of Metal (II) Complexes with Glycine-Derived Compounds

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.339.317 ◽

2011 ◽

Vol 339 ◽

pp. 317-320 ◽

Cited By ~ 1

Author(s):

Yu Ting Liu ◽

Xiao Ming Sun ◽

Da Wei Yin

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Agents ◽

Salmonella Typhi ◽

Biological Evaluation ◽

Bacterial Strains ◽

Gram Negative ◽

And Staphylococcus Aureus

A series of Ferrocene Schiff bases derived from glycine and their cobalt (II), copper (II), nickel (II), and zinc (II) metal complexes have been synthesized and characterized by IR, 1H NMR, MS and elemental analysis, the results conformed well with expected structures. The synthesized ligands, along with their metal (II) complexes, were screened in vitro for their antibacterial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that the metal (II) complexes are more potent in antibacterial activity against one or more species in comparison with those of uncomplexed ligands.

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