Sophoraflavanone G from Sophora pachycarpa Enhanced the Antibacterial Activity of Gentamycin against Staphylococcus aureus

2006 ◽  
Vol 61 (9-10) ◽  
pp. 769-772 ◽  
Author(s):  
Ali Fakhimi ◽  
Mehrdad Iranshahi ◽  
Seyed Ahmad Emami ◽  
Esam Amin-Ar-Ramimeh ◽  
Gholamreza Zarrini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Plant Extract ◽  
Acetone Extract ◽  
Enhancement Effect ◽  
Disc Diffusion ◽  
H Nmr ◽  
Broth Dilution ◽  
Mic Value ◽  
C Nmr

AbstractIn this study the enhancement effect of Sophora pachycarpa roots’ acetone extract on the antibacterial activity of gentamycin was evaluated against Staphylococcus aureus. Disc diffusion and broth dilution methods were used to determine the antibacterial activity of gentamycin in the absence and presence of plant extract and its various fractions separated by TLC. A clinical isolate of S. aureus was used as test strain. The active component of the plant extract involved in enhancement of gentamycin’s activity had Rƒ = 0.72 on a TLC plate. The spectral data (1H NMR, 13C NMR) of this compound revealed that this compound was 5,7,2′,4′-tetrahydroxy-8- lavandulylflavanone (sophoraflavanone G), previously isolated from Sophora exigua. In the presence of 0.03 μg/ mL of sophoraflavanone G the MIC value of gentamycin for S. aureus decreased from 32 to 8 μg/mL (a fourfold decrease). These results signify that the ultra-low concentration of sophoraflavanone G potentiates the antimicrobial action of gentamycin suggesting a possible utilization of this compound in combination therapy against Staphylococcus aureus.

Sophoraflavanone G from Sophora pachycarpa Enhanced the Antibacterial Activity of Gentamycin against Staphylococcus aureus

2006 ◽  
Vol 61 (11-12) ◽  
pp. 769-772
Author(s):  
Ali Fakhimi ◽  
Mehrdad Iranshahi ◽  
Seyed Ahmad Emami ◽  
Esam Amin-Ar-Ramimeh ◽  
Gholamreza Zarrini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Plant Extract ◽  
Active Component ◽  
Acetone Extract ◽  
Enhancement Effect ◽  
Disc Diffusion ◽  
H Nmr ◽  
Broth Dilution ◽  
Mic Value

Abstract In this study the enhancement effect of Sophora pachy- carpa roots’ acetone extract on the antibacterial activity of gentamycin was evaluated against Staphylococcus au­reus. Disc diffusion and broth dilution methods were used to determine the antibacterial activity of gentamy­cin in the absence and presence of plant extract and its various fractions separated by TLC. A clinical isolate of S. aureus was used as test strain. The active component of the plant extract involved in enhancement of genta- mycin’s activity had Rf = 0.72 on a TLC plate. The spec­tral data (1H NMR. 13CNMR) of this compound re­vealed that this compound was 5,7,2′,4′-tetrahydroxy-8- lavandulylflavanone (sophoraflavanone G), previously isolated from Sophora exigua. In the presence of 0.03 μg/ mL of sophoraflavanone G the MIC value of gentamy­cin for S. aureus decreased from 32 to 8 μg/mL (a four­fold decrease). These results signify that the ultra-low concentration of sophoraflavanone G potentiates the an­timicrobial action of gentamycin suggesting a possible utilization of this compound in combination therapy against Staphylococcus aureus.

scholarly journals Synthesis and Antibacterial Activity of New Spiro[thiadiazoline-(pyrazolo[3,4-d]pyrimidine)] Derivatives

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammed El Fal ◽  
Youssef Ramli ◽  
Abdelfettah Zerzouf ◽  
Ahmed Talbaoui ◽  
Youssef Bakri ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Spectroscopic Data ◽  
Heterocyclic Compounds ◽  
Dipolar Cycloaddition ◽  
Bacterial Strains ◽  
Pyrimidine Derivatives ◽  
H Nmr ◽  
Biological Interest ◽  
Mic Value

New heterocyclic compounds spiroderivatives of allopurinol of biological interest were prepared from allopurinol via thionation and 1,3-dipolar cycloaddition and were produced in high to excellent yields. These compounds were characterized on the basis of spectral and spectroscopic data (1H NMR,13C, IR, and MS). The antibacterial activity of the synthesized products was studied using bacterial strains:Staphylococcus aureus,Enterococcus faecalis,Escherichia coli, andPseudomonas aeruginosa. Compounds having an ethyl group showed the best activity with MIC value of 31.25 µg/mL againstStaphylococcus aureusandStreptococcus fasciens.

scholarly journals Antibacterial Activity of Germacrone Sesquiterpene from Curcuma Xanthorrhiza Rhizomes

2016 ◽  
Vol 12 (2) ◽  
pp. 103 ◽  
Author(s):  
Hartiwi Diastuti ◽  
Yana Maolana Syah ◽  
Lia Dewi Juliawaty ◽  
Marlia Singgih
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Enterobacter Aerogenes ◽  
Salmonella Typhi ◽  
White Crystal ◽  
H Nmr ◽  
Curcuma Xanthorrhiza ◽  
Vacuum Liquid Chromatography ◽  
C Nmr

<p>The aim of this research was to isolate and indentify the terpenoid compound from <em>Curcuma xanthorrhiza </em>rhizomes and its antibacterial activity. Isolation was carried out by using vacuum liquid chromatography and centrifugal chromatography. The structure was determined by NMR spectroscopy (<sup>1</sup>H-NMR, <sup>13</sup>C-NMR 1D and 2D), then compare with data from literatures. Antibacterial test was carried out by using  microdillution methods and evaluated against eight bacteria. They are <em>Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes</em>, <em>Pseudomonas aeruginosa, Salmonella typhi</em>, <em>Shigella dysentriae</em> and <em>Vibrio cholerae</em>. The result showed that the isolate was a white crystal which was indetified as germacron-type sesquiterpene. Germacron have highest activity againts <em>P. aeruginosa, </em>MIC<em> </em>15.6 µg/mL <em>and </em>MBC 31.2 µg/mL.</p>

scholarly journals Antibacterial Activity of Germacrone Sesquiterpene from Curcuma Xanthorrhiza Rhizomes

2016 ◽  
Vol 12 (2) ◽  
pp. 103
Author(s):  
Hartiwi Diastuti ◽  
Yana Maolana Syah ◽  
Lia Dewi Juliawaty ◽  
Marlia Singgih
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Enterobacter Aerogenes ◽  
Salmonella Typhi ◽  
White Crystal ◽  
H Nmr ◽  
Curcuma Xanthorrhiza ◽  
Vacuum Liquid Chromatography ◽  
C Nmr

<p>The aim of this research was to isolate and indentify the terpenoid compound from <em>Curcuma xanthorrhiza </em>rhizomes and its antibacterial activity. Isolation was carried out by using vacuum liquid chromatography and centrifugal chromatography. The structure was determined by NMR spectroscopy (<sup>1</sup>H-NMR, <sup>13</sup>C-NMR 1D and 2D), then compare with data from literatures. Antibacterial test was carried out by using  microdillution methods and evaluated against eight bacteria. They are <em>Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes</em>, <em>Pseudomonas aeruginosa, Salmonella typhi</em>, <em>Shigella dysentriae</em> and <em>Vibrio cholerae</em>. The result showed that the isolate was a white crystal which was indetified as germacron-type sesquiterpene. Germacron have highest activity againts <em>P. aeruginosa, </em>MIC<em> </em>15.6 µg/mL <em>and </em>MBC 31.2 µg/mL.</p>

scholarly journals GC-MS Analysis and Antibacterial Activity of different Solvent Extracts of Shuteria involucrata

2018 ◽  
Vol 7 (2) ◽  
pp. 116-120
Author(s):  
Senthamizh Selvan N ◽  
◽  
Isaiah S ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Disc Diffusion ◽  
Plant Materials ◽  
Gram Negative ◽  
Good Antibacterial Activity ◽  
Ms Analysis ◽  
Diffusion Technique

The present study was focused to examine the presence of phytoconstituents in the ethanolic extract of Shuteria involucrata plant using GC-MS analysis and Antibacterial activity. The GC-MS analysis of S. involucrata leaf was performed using Agilent 6890-JEOL GC-Mate-II Mass Spectrometer. The result of the study showed the presence of six bioactive compounds in the ethanolic extract. The antimicrobial activity was carried out by disc diffusion technique against the four selected pathogens. Among the four, tested for Antibacterial Activity Staphylococcus aureus, and Pseudomonas aeruginosa and were more susceptible to the extract, whereas the others are less susceptible. Ethanol and methanol extracts of plant materials exhibited good antibacterial activity against gram positive, gram negative bacterias

scholarly journals Comparison of In Vitro Antibacterial Activity of Epaltes divaricata and Vetiveria zizanioides against Methicillin-Resistant Staphylococcus aureus

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Hasanga Rathnayake ◽  
Manikkuwadura Hasara Nethmini De Zoysa ◽  
Ruwani Punyakanthi Hewawasam ◽  
Weerasinghe Mudiyanselage Dilip Gaya Bandara Wijayaratne
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Hexane Extract ◽  
Vetiveria Zizanioides ◽  
Methicillin Resistant ◽  
Water Extracts ◽  
Mrsa Strains ◽  
Ethanol Extracts ◽  
Mic Value

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital- and community-acquired infections worldwide. Therefore, this study was conducted to explore the antibacterial activity of the two medicinal plants Epaltes divaricata and Vetiveria zizanioides against strains of MRSA which were isolated from patients with skin and soft tissue infections. Hexane, ethanol, and water extracts of E. divaricata (whole plant) and V. zizanioides (roots) were prepared. Clinical isolates of MRSA strains (n = 20) were used for the study. Bacterial susceptibility was tested using a disc diffusion assay. Minimum inhibitory concentration (MIC) was determined by a broth microdilution method. Vancomycin was used as the positive control. Hexane, ethanol, and water extracts of E. divaricata showed inhibitory zones against MRSA. Except for water extract, both hexane and ethanol extracts of V. zizanioides showed inhibitory zones. MIC ranges of hexane, ethanol, and water extracts in E. divaricata were 0.012–0.32 mg/mL, 0.019–2.4 mg/mL, and 0.019–0.48 mg/mL, respectively. Respective MIC ranges of hexane and ethanol extracts of V. zizanioides were 0.003–0.032 mg/mL and 0.019–2.4 mg/mL. The hexane extract of V. zizanioides inhibited 55% of the selected MRSA strains at a relatively low MIC value of 0.012 mg/mL. The hexane extract of both plants demonstrated inhibition of 75% of MRSA strains at a MIC value of 0.064 mg/mL. Ethanol extract of V. zizanioides and E. divaricata, respectively, inhibited 70% and 45% of MRSA strains at the MIC of 0.096 mg/mL, whereas water extract of E. divaricata inhibited 80% of MRSA strains at the same MIC. Both E. divaricata and V. zizanioides were equally effective against MRSA at a MIC of 0.064 mg/mL. But V. zizanioides was more effective since the hexane extract inhibited more than 50% of MRSA strains at significantly a lower MIC value of 0.012 mg/mL. Fractionation, purification, and identification of active compounds will warrant further evaluation of the therapeutic potential of both plant extracts.

scholarly journals Component Analysis of Multipurpose Contact Lens Solutions To Enhance Activity against Pseudomonas aeruginosa and Staphylococcus aureus

2016 ◽  
Vol 60 (7) ◽  
pp. 4259-4263 ◽  
Author(s):  
Leo Lin ◽  
Janie Kim ◽  
Hope Chen ◽  
Regis Kowalski ◽  
Victor Nizet
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Contact Lens ◽  
Dilution Method ◽  
Microbial Keratitis ◽  
Content Type ◽  
Contact Lens Solutions ◽  
Contact Lens Solution ◽  
Broth Dilution ◽  
Solution Formulation

ABSTRACTMore than 125 million people wear contact lenses worldwide, and contact lens use is the single greatest risk factor for developing microbial keratitis. We tested the antibacterial activity of multipurpose contact lens solutions and their individual component preservatives against the two most common pathogens causing bacterial keratitis,Pseudomonas aeruginosaandStaphylococcus aureus. Thein vitroantibacterial activity of five multipurpose contact lens solutions (Opti-Free GP, Boston Simplus, Boston Advance, Menicare GP, and Lobob) was assayed by the standard broth dilution method. Synergy between the preservative components found in the top performing solutions was assayed using checkerboard and time-kill assays. The ISO 14729 criteria and the standard broth dilution method were used to define an optimized contact lens solution formulation against a clinical panel of drug-susceptible and drug-resistantP. aeruginosaandS. aureusstrains. Preservatives with the biguanide function group, chlorhexidine and polyaminopropylbiguanide (PAPB), had the best antistaphylococcal activity, while EDTA was the best antipseudomonal preservative. The combination of chlorhexidine and EDTA had excellent synergy againstP. aeruginosa. A solution formulation containing chlorhexidine (30 ppm), PAPB (5 ppm), and EDTA (5,000 ppm) had three to seven times more antipseudomonal activity than anything available to consumers today. A multipurpose contact lens solution containing a combination of chlorhexidine, PAPB, and EDTA could help to reduce the incidence of microbial keratitis for contact lens users worldwide.

scholarly journals Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

2020 ◽  
pp. 48-57
Author(s):  
Ebrahim Sande ◽  
Danstone Lilechi Baraza ◽  
Selline Ooko ◽  
Peter Kuloba Nyongesa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Ganoderma Lucidum ◽  
Methicillin Resistance ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

scholarly journals TRANSFORMATION OF ETHYL-P-METHOXYCINNAMATE TO P –METHOXYCINNAMIC ACID FROM KENCUR (Kaempheria galanga L.) AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 13 (2) ◽  
pp. 176
Author(s):  
Muhamad Salman Fareza ◽  
Rehana Rehana ◽  
Nuryanti Nuryanti ◽  
Didin Mujahidin
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Separation And Purification ◽  
E Coli ◽  
H Nmr ◽  
Alkaline Conditions ◽  
Methoxycinnamic Acid ◽  
Vacuum Liquid Chromatography ◽  
Hydrolysis Of ◽  
C Nmr

This study aimed to evaluate the antibacterial properties of ethyl-<em>p</em>-methoxycinnamate and <em>p</em>-methoxycinnamate acid from Kaempheria galanga L. Ethyl-<em>p</em>-methoxycinnamate was isolated from the <em>n</em>-hexane rhizome extract of <em>Kaempheria galanga L</em>. Separation and purification of this compound was carried out with vacuum liquid chromatography and column chromatography. Hydrolysis of ethyl-<em>p</em>-methoxycinnamic under alkaline conditions obtained <em>p</em>-methoxycinnamic acid with a good yield of 85 %. The structure of the compounds were charactrized with IR, NMR spectrophotometer (<sup>1</sup>H-NMR and <sup>13</sup>C-NMR) and mass spectrophotometer. The antibacterial properties of the compounds were evaluated using microdilution methods against <em>B. cereus</em> ATCC 11778, <em>L. monocytogenes</em> ATCC 7644, <em>E. coli</em> ATCC 25922, <em>S. enterica sv Typhimurium</em> ATCC 14028, and <em>E. aerogenes</em> ATCC 13048. The compounds showed weak antibacterial properties. Only ethyl <em>p</em>-methoxycinnamate showed the strongest antibacterial activity, especially against <em>B. cereus</em> ATCC 11778 bacteria with MIC values of 62.5 mg /mL. The change of the functional groups provided no significant impact on the antibacterial activity.

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