Isolation and identification of microorganisms from water samples in rivers state university of science and technology tanks

2020 ◽  
Vol 9 ◽  
Author(s):  
Otu Jonathan Oyim ◽  
Buekor Nyornebari Grace ◽  
B. S. Baranu
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Science And Technology ◽  
Bacterial Count ◽  
Water Tank ◽  
State University ◽  
Isolation And Identification ◽  
Klebsiella Species ◽  
Rivers State

Safe drinking water is vital for the survival of humans and other living organisms that depend on it, but when pathogens are present, it limits the use of the water. The microorganisms present in Rivers State University of Science and Technology hostels water tanks were isolated and identified; and the effects of the microbes were determined. Six (6) water samples gotten from different tanks were analysed for their microbiological quality for a period of three (3) weeks. Fungal colonies were isolated using the wet mount technique on commercially prepared Potato dextrose agar (PDA), the multiple tube fermentation technique with MacConkey broth and Nutrient agar were used as media for the isolation and identification of the bacterial colonies. From the analysis, the bacterial count was higher than the fungal count. This indicates faecal contamination ranging between 5 x 100 to 2.6 x 101 coliform (MPN) per 100ml. Also, the total heterotrophic bacterial count ranged from 4.05 x 106 to 1.14 x 107 with the fungal count being the lowest by having 7.0 x 104 to 1.8 x 105. The microorganisms isolated from the water tank samples were Aspergillus species, Penicillium species, Rhizopus species, Mucor species, Escherichia species, Enterobacter species, Bacillus species, Pseudomonas species and Klebsiella species. Klebsiella species which are high across all samples have been known to cause destructive pneumonia. The occurrence of these pathogenic bacteria in drinking water may increase the risks of water-related diseases and health problems to the susceptible residents

Detection of Coliform, faecal Coliform And Total Bacterial Count From Drinking Water Of Varanasi (U.P.) India

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Alok Kumar Singh ◽  
Anand Prakash Singh ◽  
Sanjay Srivastava
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Bacterial Count ◽  
Most Probable Number ◽  
Total Bacterial Count ◽  
Indicator Organisms ◽  
Cross Sectional ◽  
Probable Number ◽  
Hand Pump

In India source of drinking water at Varanasi city for common people are tap water, well, hand pump, Ganga river and stored tank water collected from bore well. All water samples were studied to assess their bacteriological characteristics and suitability for potable purposes. A cross-sectional epidemiological method was adopted to investigate the drinking water of six different sites of Varanasi city. The bacteriological examination of water samples included the most probable number of presumptive coliforms, faecal coliforms, and total bacterial count. The results showed that the total coliform count was detected in all the site. In all the methods coliforms presence was indicated. Maximum number of coliform observed in all the seasons, were from river and well water followed by hand pump, tap water and stored tank. The most common group of indicator organisms used in water quality monitoring are coliforms. These organisms are representative of bacteria normally present in the intestinal tract of mammals including human. Contamination of water may occur through different way like sewage disposal in the river, seepage of bathing near sites, fecal excreta of human, bird and other animals. Improving and expanding the existing water treatment and sanitation systems are more likely to provide good, safe and sustainable sources of water in the long term.

Evaluation of methods for isolation of DNA for PCR based identification of pathogenic bacteria from pure cultures and water samples

2009 ◽  
Vol 59 (3) ◽  
pp. 619-620
Author(s):  
K. Horáková ◽  
H. Mlejnková ◽  
P. Mlejnek
Keyword(s):  
Water Samples ◽  
Pathogenic Bacteria ◽  
Science And Technology ◽  
Pure Cultures ◽  
Water Science ◽  
Evaluation Of Methods

Water Science and Technology 58(5), 995–999 Publisher's note. We regret that the version of this article used in production did not incorporate a number of corrections and clarifications. The corrected versions of the text are as given below.

scholarly journals The Burden of Poor Household Drinking-Water Quality on HIV/AIDS Infected Individuals in Rural Communities of Ugu District Municipality, Kwazulu-Natal Province, South Africa

2019 ◽  
Author(s):  
C.M.N Khabo-Mmekoa ◽  
M.N.B Momba
Keyword(s):  
Drinking Water ◽  
Rural Communities ◽  
Proteus Mirabilis ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Rrna Gene ◽  
Aids Patients ◽  
Shigella Boydii ◽  
Stool Specimens ◽  
Hiv Aids

ABSTRACTThis aim of this study was to ascertain whether household container-stored drinking water might play a role in the diarrhoeic conditions of HIV/AIDS patients and non-HIV-infected individuals of the rural communities who attended the Ugu District Municipal hospitals. Water samples were collected from the standpipes and household containers, and stool specimens were obtained from HIV/AIDS-positive and non-HIV/AIDS patients with diarrhoea. Significant correlations were established between the incidence of potentially pathogenic bacteria isolated from chlorinated household-stored water, and in stool specimens of HIV-positive patients with diarrhoea (r = P < 0.05). A combination of molecular analysis targeting the 16S rRNA gene and the restriction fragment length polymorphism and sequence analysis of the amplified gene for differentiating between species and strains of the bacterial pathogens was also applied to isolates obtained from stored-water samples and stool specimens. Similar sequences of Klebsiella spp., K. pneumoniae, Escherichia coli, E. coli O55: H7, Proteus mirabilis, and Shigella boydii were identified in both stored water and stools of HIV/AIDS-positive patients with diarrhoea. With the exception of Proteus mirabilis, none of these pathogens were identified in stool specimens of non-HIV-infected individuals with diarrhoea.

PATHOGENS FROM DRINKING WATER

2018 ◽  
Vol 20 (04) ◽  
pp. 760-765
Author(s):  
Mohammad Din ◽  
Zafar Ahmad ◽  
Abdul Aleem ◽  
G.S. Pirkani ◽  
Amir Mohammad ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Clinical Medicine ◽  
Membrane Filter ◽  
Wide Spectrum ◽  
Bacterial Load ◽  
Antibiotic Sensitivity ◽  
Viable Count ◽  
Serological Tests

… Objectives: Main objective was to check drinking water for pathogenic bacterialload, their resistance to antimicrobials and to create awareness among the people of Quetta cityabout safe drinking water. Place and Duration of Study: The study was conducted inDepartment of Microbiology Bolan Medical Complex Hospital Quetta during the hot season inQuetta City (June- September 2013). Methodology: One hundred and twenty five (125) tapewater samples were collected aseptically in 200 ml sterile caped glass bottles from differentlocalities of Quetta city. Samples were passed through mille pore assembly containing 0.45 μmpore-sizecellulose nitrate sterile membrane filter (MF).Viable count technique was used forenumeration of water samples having high bacterial burden. Serological tests and analyticalprofile index API-20E (Biomerieux France) were used to identify pathogens according to themanufacturer’s directions. Standardized antibiotic sensitivity test was performed on MuellerHinton agar using disc diffusion Kirby Bauer technique and McFarland Turbidity Standardmethod 0.5 following CLSI protocols. Results: Out of hundred and twenty five (125) tape watersamples 110 (88 %) showed highly pathogenic bacterial load, in which the most prominentorganism was E.coli 36 (28.8 %), followed by Enterobacter 35 (28 %), Klebsiella 24 (19.2 %),Pseudomonas, 10 (08 %), and Salmonella 05 (04 %). All pathogens in this study expressed a highlevel of resistance to antimicrobials that are commonly used in clinical medicine i.e. Tetracycline,Gentamycin, Sulphamethaxazole, Piperacillin, Ampicillin, Augmentin and Imipenam etc. Only 15(12 %) samples were pathogens free. Conclusion: Among drinking water samples the presenceof pathogenic bacteria (88%) is alarming for public health authorities. The emergence ofresistance and decreasing level of susceptibility of pathogens to a wide spectrum ofantimicrobials is a matter of great concern, because it may limit the availability of antimicrobialsfor clinical management of water born outbreaks in future.

scholarly journals Abundance of pathogenic Escherichia coli, Salmonella typhimurium and Vibrio cholerae in Nkonkobe drinking water sources

2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Salmonella Typhimurium ◽  
Vibrio Cholerae ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Water Sources ◽  
E Coli ◽  
Drinking Water Sources

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.

scholarly journals Qualitative analysis of drinking water through the most probable number (MPN) method

2015 ◽  
Vol 3 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Tasnia Ahmed ◽  
Sagar Baidya ◽  
Mrityunjoy Acharjee ◽  
Tasmina Rahman
Keyword(s):  
Drinking Water ◽  
Qualitative Analysis ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Disease Outbreaks ◽  
Alcaligenes Faecalis ◽  
Health Concern ◽  
Most Probable Number ◽  
Probable Number ◽  
Unsafe Water

Water borne disease outbreaks associated with the drinking of unsafe water, containing pathogenic bacteria of fecal origin, is common in densely populated countries like Bangladesh. Present study was attempted to detect indicator bacteria from drinking water samples for the presumptive occurrence of fecal contaminations that are responsible for health associated problems. Therefore, a laboratory scale qualitative analysis through most probable number (MPN) method was employed. The indicator bacterium Escherichia coli were detected in 24 water samples out of 75 samples as revealed consequently by the presumptive, confirmed and completed tests of MPN method. Other Gram negative bacteria found in the samples included Alcaligenes faecalis, Pseudomonas spp., Klebsiella spp. and Proteus spp. Almost all of the identified bacteria showed resistance against commonly used antibiotics which is of significant health concern. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22745 Stamford Journal of Microbiology, Vol.3(1) 2013: 9-16

scholarly journals Assessment of microbiological quality and contamination level of Hospital water samples collected from storage points

2020 ◽  
Vol 11 (3) ◽  
pp. 4451-4462
Author(s):  
Rajani Chowdary Akkina ◽  
Payala Vijayalakshmi ◽  
Vidyullatha Peddireddy
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Hospital Environment ◽  
Diffusion Method ◽  
Most Probable Number ◽  
Contamination Level ◽  
Potential Threat ◽  
Drinking Water Contamination ◽  
Probable Number

Drinking water contamination at the level of storage points poses a potential threat to the hospital environment as it can lodge some resistant pathogenic microorganisms that may cause hospital acquired infections. The study analyzed the physico-chemical and microbiological parameters of drinking water samples collected from the main water storage points from ten different local hospitals in and around Visakhapatnam using standard protocols. The samples were processed within 2 hours after collection and the identification of pathogenic bacteria was performed through Most Probable Number (MPN) method, cultural characteristics and biochemical reactions. Antimicrobial susceptibility test was carried out by Agar well diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. In the current study, all the tested ten water samples cross the permissible MPN count indicating that the water samples were not potable for drinking purpose and needs further and better disinfection procedures. Among the isolated pathogens, Pseudomonas aeruginosa exhibited highest sensitivity to antibiotics imipenem (50%) and tetracycline (50%) and resistance towards other tested antibiotics, whereas E.coli showed 100% susceptibility to imipenem and 100% resistance to ampicillin. Out of ten isolated strains of Proteus species, majority have exhibited 80% resistance to ampicillin and Tetracycline and 80% sensitivity to imipenem. All the four isolated strains of Shigella species expressed 100% resistance to ampicillin and 75% sensitivity to imipenem, meropenem, azithromycin, linezolid, vancomycin and chloramphenicol. The five isolated strains of Vibrio species showed 100% resistance to ampicillin and 80% sensitivity to imipenem, doxycycline and tetracycline. Greater part of the isolates were multidrug resistant isolates.

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