scholarly journals Potential Analysis Of Toxoplasmosis Distribution In Wild Cats (Felis silvestris) In Some Markets Of Sidoarjo District Through Microscopic Identification Of Toxoplasma gondii

2020 ◽  
Vol 3 (2) ◽  
pp. 59-64
Author(s):  
Rizal Zakaria ◽  
Syahrul Ardiansyah
Keyword(s):  
Toxoplasma Gondii ◽  
Environmental Factors ◽  
Parasitic Infection ◽  
Zoonotic Disease ◽  
Felis Silvestris ◽  
Potential Analysis ◽  
Microscopic Identification ◽  
Stray Cats ◽  
Wild Cats

There are many diseases caused by parasitic infection, one of them is toxoplasmosis which is a zoonotic disease. This study was to determine the potential spread of wild cat toxoplasmosis in several markets. The sample used in this study were 24 stray cats taken from the Larangan market, Suko market and Sukodono market in Sidorajo district, using a method of determining the size of the sample based on incidental sampling. Sample were examined using the floating method.The prevalence result obtained from Larangan market (37,5%), Suko market (37,5%) and Sukodono market (12,5%). Prevalence result in Larangan market and Suko market greater than in Sukodono market, this is due to environmental factors in Sukodono market cleaner and better sanitation. Human can become infected with oocyst if they consume food or inhale particle contaminated with T.gondii parasites. High number of cat with oocyst T.gondii can be caused by a dirty environment.

scholarly journals Diagnostic methods and protocols used in investigating Toxoplasma gondii in humans: A review

2021 ◽  
Vol 2 (04) ◽  
pp. 181-186
Author(s):  
Shmeeran Ismael
Keyword(s):  
Toxoplasma Gondii ◽  
Clinical Features ◽  
Molecular Diagnosis ◽  
Parasitic Infection ◽  
Short Review ◽  
Zoonotic Disease ◽  
Accurate Diagnosis ◽  
Diagnostic Methods ◽  
Immunocompromised Patients

Toxoplasmosis, a zoonotic disease, is a parasitic infection caused by a coccidian protozoan Toxoplasma gondii. In immunocompetent people, the infection is asymptomatic, while it can produce serious signs in immunocompromised people and in developing fetuses. Accurate diagnosis is dependent, mainly, on the clinical features. However, in immunocompromised patients, the diagnosis is very difficult and may lead to misdiagnosis and improper treatment. Today, molecular diagnosis and serotyping are widely used for the diagnosis of T. gondii in many countries. The aim of the present short review is to highlight the current diagnostic methods and protocols that are used for the diagnosis of T. gondii in humans.

Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Majorca, Balearic Islands, Spain

2009 ◽  
Vol 165 (3-4) ◽  
pp. 323-326 ◽  
Author(s):  
Javier Millán ◽  
Oscar Cabezón ◽  
Marcela Pabón ◽  
J.P. Dubey ◽  
Sonia Almería
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Balearic Islands ◽  
Felis Silvestris ◽  
Felis Silvestris Catus ◽  
Feral Cats

scholarly journals Seroprevalence of Toxoplasma gondii antibodies of stray cats in Garmsar, Iran

2013 ◽  
Vol 39 (2) ◽  
pp. 306-308 ◽  
Author(s):  
Maysam Tehrani-sharif ◽  
Sina Jahan ◽  
Seyed Mohsen Alavi ◽  
Mohsen Khodami
Keyword(s):  
Toxoplasma Gondii ◽  
Stray Cats

scholarly journals Genetic Characterization of Toxoplasma gondii Isolates and Toxoplasmosis Seroprevalence in Stray Cats of İzmir, Turkey

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104930 ◽  
Author(s):  
Hüseyin Can ◽  
Mert Döşkaya ◽  
Daniel Ajzenberg ◽  
H. Gökhan Özdemir ◽  
Ayşe Caner ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Stray Cats ◽  
Toxoplasma Gondii Isolates

scholarly journals Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites

2018 ◽  
Vol 6 (9) ◽  
pp. 1577-1580
Author(s):  
Nihal A. Hanafy ◽  
Mohamed S. Badr ◽  
Ghada M. Nasr
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Parasitic Infection ◽  
Clinical Samples ◽  
Molecular Assays ◽  
Low Concentrations ◽  
Target Dna ◽  
Primer Sets ◽  
Amplification Failure ◽  
Reference Samples

BACKGROUND: Toxoplasma gondii is a common parasitic infection of humans. Infection is usually mild. Serious complications can occur in pregnant and immunocompromised patients. AIM: The present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples. METHODS: The target DNA was provided in 8 different quantities. RESULTS: Amplification failure was reported only with the cPCR in samples of low concentrations using both primer sets. Quantitative PCR detected the 8 different dilutions of the purified Toxoplasma gondii using the 2 sets of primers while cPCR was sensitive to detect only 6 different dilutions. CONCLUSION: Generally real-time quantitative molecular assays, is easy to use method compared to conventional PCR assay and produces more reliable results within only one hour time but still the possible application of qPCRs in routine diagnosis necessitates analysis of a large number of clinical samples in further studies to make the proper choice.

scholarly journals Seropathological Diagnosis of Toxoplasma gondii in Stray Cats in Baghdad Province

2014 ◽  
Vol 38 (1) ◽  
pp. 92-98
Author(s):  
Mohammed J. Alwan
Keyword(s):  
Toxoplasma Gondii ◽  
Alveolar Space ◽  
Internal Organs ◽  
Blood Samples ◽  
Pathological Lesions ◽  
Stray Cats ◽  
Significant Difference ◽  
Adult Cat ◽  
The Brain ◽  
Local Necrosis

The current study was performed an investigation in seropositive stray cats with Toxoplasma gondii and to correlate the results of pathological lesions with seropositive results of the cats. To achieve these goals, fifty blood samples and specimens from internal organs (liver, spleen, brain, kidney, intestine and lung) of stray cats were collected from different area of Baghdad Province during the period 1.10.2011-1.10.2012. Seropositive ELISA-IgG was demonstrated that 66 % of stray cats while female expressed (75 %) of seropositive ELISA-IgG were higher than male (30% ) and animals with average age 2months showed high percentage of seropositive ELISA-IgG (100%) as compared with age (adult cat) that expressed( 63.82 %) of seropositive. There was a significant difference (P≤0.01) among positive cases to anti-Toxoplasma ELISA-IgG. Severe pathological lesions were noticed in the lungs ,livers and intestines of animals that expressed high (optical density) (OD) of anti-Toxoplasma IgG ,in addition ,tachyzoits intracytoplasm of alveolar macrophages and hepatocytes as well as free zoites in alveolar space of the lung, were reported. Local necrosis with tachyzoites was seen in the brain of the cats, in addition to mineralization. On bases of the presence of pathological lesions in cats that expressed seropositive anti-Toxoplasma antibodies, it can be conclude that T.gondii is responsible for the appearance of inflammatory reaction in the internal organs of cats and there is a correlation between seropositive and pathological lesions of T.gondii infection and this parasite is highly distributed in Baghdad stray cats and it may be an important cause of abortion in the women.

Seroprevalence of Toxoplasma Gondii Among Stray Cats in Qatar

2016 ◽  
Author(s):  
Sonia Boughattas ◽  
Aarti Sharma ◽  
Marawan Abu-Madi
Keyword(s):  
Toxoplasma Gondii ◽  
Stray Cats
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