scholarly journals Karakteristik Fisikokimia dan Identifikasi Komposisi Asam Lemak Minyak Testa Kelapa [Physicochemical Characteristics and Identification of Fatty Acid Composition Coconut Testa Oil]

Buletin Palma ◽  
2020 ◽  
Vol 21 (2) ◽  
pp. 81
Author(s):  
Ardi Kurniawan Makalalag ◽  
Anton Muis ◽  
Nicolas Tumbel
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Methyl Ester ◽  
Lauric Acid ◽  
Caprylic Acid ◽  
Acid Methyl Ester ◽  
Capric Acid ◽  
Acid Methyl

<p>Testa is a byproduct of the coconut flour industrial processing process and has not been used optimally. Testa is the outer layer of coconut flesh which is brown. peeled with a thickness of about 2mm, dried, and taken the oil. The purpose of this study was to see the potential that can be processed from coconut testa by identifying the physical and chemical properties of testa oil and to determine the composition of its fatty acid content. The research was carried out in 2019 and carried out at the Manado Industrial Research and Standardization Center Laboratory. The results showed a water content value of 0.2148%; free fatty acids 2.02%; the peroxide number is 0.4107 Mek O2/Kg; iodine number 14.452 g iodine/100g; and the saponin number 258.46 mg KOH/g. The results of testing the fatty acid composition of testa oil using a Gas Chromatography-Mass Spectrometry (GCMS) tool were obtained, eight types of fatty acids consisting of medium-chain fatty acids and long-chain fatty acids, with the most dominant percentage being Lauric acid methyl ester (C12 - Lauric acid) of 41.59% which is a group of medium-chain fatty acids. Overall, the fatty acids obtained are Caprylic acid methyl ester (C8 - caprylic acid) 5.43%, Capric acid methyl ester (C10 - capric acid) 4.68%, Lauric acid methyl ester (C12 - lauric acid) 41, 59%, Myristic acid methyl ester (C14 - myristic acid) 18.87%, Palmitic acid methyl ester (C16 - palmitic acid) 11.87%, Linoleic acid methyl ester (C18 - linoleic acid) 1.67%, Oleic acid methyl ester (C18 - oleic acid) 11.88%, Stearic acid methyl ester (C20 - stearic acid) 4.01%. The results obtained show that coconut testa oil has the potential to be developed into functional food, cosmetic and pharmaceuticals products .</p><p align="center"><strong>ABSTRAK</strong></p><p>Testa adalah produk samping dari proses pengolahan industri tepung kelapa, dan belum dimanfaatkan secara optimal. Testa merupakan lapisan luar daging buah kelapa yang berwarna coklat. dikupas dengan ketebalan sekitar 2mm, dikeringkan dan dikeluarkan minyaknya. Tujuan penelitian ini adalah untuk melihat potensi yang dapat diolah dari testa kelapa dengan cara mengidentifikasi sifat fisik dan kimia dari minyak testa dan untuk mengetahui komposisi kandungan asam lemaknya. Penelitian dilaksanakan pada tahun 2019 dan dilakukan di Laboratorium Balai Riset dan Standardisasi Industri Manado. Hasil penelitian diperoleh nilai kadar air 0,2148%; asam lemak bebas 2,02%; bilangan peroksida sebesar 0,4107 Mek O<sub>2</sub>/Kg; bilangan iod 14,452 g iod/100g; dan bilangan penyabunan 258,46 mg KOH/g. Hasil pengujian komposisi asam lemak minyak testa menggunakan alat <em>Gas Chromatography-Mass Spectrometry</em> (<em>GCMS</em>) diperoleh, delapan jenis asam lemak penyusun yang terdiri dari asam lemak rantai medium dan asam lemak rantai Panjang, dengan persentase yang paling dominan adalah Lauric acid methyl ester (C12 – Asam laurat) sebesar 41,59% yang merupakan golongan dari asam lemak rantai medium. Secara keseluruhan asam lemak yang diperoleh adalah, Caprylic acid methyl ester (C8 – asam kaprilat) 5,43%, Capric acid methyl ester (C10 – asam kaprat) 4,68%, Lauric acid methyl ester (C12 – asam laurat) 41,59%, Myristic acid methil ester (C14 – asam miristat) 18,87%, Palmitic acid methyl ester (C16 – asam palmitat) 11,87%, Linoleic acid methyl ester (C18 – asam linoleat) 1,67%, Oleic acid methyl ester (C18 – asam oleat) 11,88%, Stearic acid methil ester (C20 – asam stearat) 4,01%. Hasil yang diperoleh menunjukkan minyak testa kelapa memiliki potensi yang dapat dikembangkan menjadi produk pangan fungsional,  kosmetik, maupun farmasi.</p>

scholarly journals Studying the Physicochemical Properties and Isolation of Unsaturated Fatty Acids from Edible Oils by GC-MS and Argentated Silica Gel Chromatography

2021 ◽  
pp. 346-362
Author(s):  
Salim Najmaldain Saber ◽  
Hikmat Ali Mohamad ◽  
Madzlan Aziz
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Methyl Ester ◽  
Physicochemical Properties ◽  
Olive Oil ◽  
Unsaturated Fatty Acids ◽  
Acid Methyl Ester ◽  
Acid Methyl

The core objective of this study was to investigate the physicochemical characteristics and fatty acid composition of the oils of sunflower, olive, virgin coconut and ginger oils, as well as the separation of their unsaturated fatty acids. The data indicated a significant variation in physicochemical properties (acid, saponification, ester, and iodine values) among oils. Transesterification process was carried out at a molar ratio of 1:7:0.1 of oil: methanol: KOH. Fatty acid methyl esters of oils were analyzed by infrared (IR) and gas chromatography–mass (GC-MS) spectrometry. Twelve fatty acids were identified, where the major fatty acid of  olive oil was found to be  oleic acid (89%), whereas those of sunflower and ginger oils were linoleic acid (80.9 %) and (79.3 %), respectively. Sunflower and olive oils were fractionated by 25% silver nitrate-impregnated silica gel column chromatography. By this method, linoleic acid methyl ester from sunflower and oleic acid methyl ester from olive oil were isolated with high purity percentages and yields. This study is significant for the development of food and pharmaceutical products.

scholarly journals Possibility of oil seeds in feeding dairy cows

2014 ◽  
pp. 67-73
Author(s):  
Ágnes Süli ◽  
Béla Béri ◽  
János Csapó ◽  
Éva Vargáné Visi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Lauric Acid ◽  
Myristic Acid ◽  
Caprylic Acid ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Capric Acid

The efforts to modify the fatty acid composition of milk have intensified with health conscious nutrition coming to the forefront.This experiment of ours was designed to investigate to what extent the natural-based feed additives, such as oilseeds, can influence the fatty acid composition of cow’s milk.Further information was gained about feeding of oilseeds in specific amounts to be fitted into the technology of a large-scale dairy farm in practice. The feed supplements were whole, untreated rapeseed and whole, untreated linseed, as part of a total mixed ration. In case of saturated fatty acids when supplementing with whole rapeseed the most significant change was observable in the concentration of the caprylic acid, capric acid, undecylic acid, lauric acid, myristic acid, stearic acid. In case of unsaturated fatty acids the quantity of oleic acid enhanced considerably. When observating the feeding with whole linseed the concentration of many saturated fatty acids lowered (caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid). The quantity of some unsaturated fatty acids was showing a distinct rise after feeding with linseed, this way the oleic acid, α-linolenic acid, conjugated linoleic acid, eicosadienoic acid. The aim of the study was to produce food which meets the changed demands of customers, as well.

scholarly journals Reduction of Detrimental Effect of Soybean Oil in-vivo Using Watermelon White Rind Extract

2019 ◽  
pp. 1-6
Author(s):  
Hanan M. El-Ghandour
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Methyl Ester ◽  
Soybean Oil ◽  
Aqueous Extract ◽  
Calcium Level ◽  
Acid Methyl Ester ◽  
Negative Control ◽  
Acid Methyl

Aim: To study the effect of white rind extract on decreasing soybean oil impact on calcium and phosphorous blood levels in vivo. Method: Dried watermelon white rind was directed to mycotoxin and elemental determinations to assure its safe usage. Soybean oil was subjected to fatty acid and GC-MS analysis. Biological experiment was conducted using male albino rats fed diet prepared by soybean oil and supplied with aqueous watermelon white rind extract for two months’ interval period. At the end of the experiment, the calcium and phosphorus in blood were determined. Results: The rind was free from aflatoxin and ochratoxin. Watermelon white rind aqueous extract contained iron, copper, potassium, chromium and selenium at concentration ranges of 3.4, 0.53, 45.51, 0.0142 and 0.0985 ppm, respectively. Soybean oil had free fatty acid, peroxide value, iodine number and anisidine value of 0.43%, 13.62 meq O2/Kg, 132 and 0.7, respectively. GC-MS analysis of soy oil ascertained the presence of twenty-four compounds: linoleic acid, methyl ester (25.27%), monensin (15.75%), elaidic acid (9.24%), nonadecanoic acid, methyl ester (7.04%), cis-13-eicosenoic acid (4.92%), cis-vaccenic acid (4.68%), linoleic acid (4.67%), palmitoleic acid (4.46%), 9-tetradecenal (4.42%) and cysteine (4.18%)were the most predominant. Fatty acid profile of the oil showed that the ratio of saturated fatty acid to unsaturated fatty acids was 1:5. Conclusion: Rats fed diet prepared by soybean oil had a decreased calcium level in comparison with negative control (p<0.05). Supplementation with watermelon white rind aqueous extract rendered calcium level to normal status as negative control. Phosphorus level wasn’t affected by soya oil.  

scholarly journals COMPOSITION AND QUANTIFICATION OF FATTY ACIDS PRODUCED BY Xylaria sp. DAP KRI-5

2021 ◽  
Vol 20 (1) ◽  
pp. 31-41
Author(s):  
Ahmad Fathoni ◽  
Muhammad Ilyas ◽  
Praptiwi Praptiwi ◽  
Andi Saptaji Kamal ◽  
Lukman Hafid ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Acid Methyl

Asam lemak mempunyai nilai guna komersial sebagai suplemen makanan, produk farmasi dan sumber energi terbarukan. Sumber asam lemak sebagian berasal dari hewan, tumbuhan, dan jamur endofit. Penelitian ini bertujuan untuk menganalisis kandungan dan komposisi asam lemak dari jamur endofit  Xylaria sp. DAP KRI-5. Asam lemak didapatkan dari fraksi n-heksana yang dihasilkan dari partisi ekstrak etil asetat Xylaria sp. DAP KRI-5 dengan n-heksana: metanol (1:1). Turunan FAME (Fatty acid methyl ester) dari asam lemak didentifikasi dengan menggunakan GC-MS. Kandungan asam lemak dari fraksi n-heksana Xylaria sp. DAP KRI-5 adalah 26,39% (b/b) ekstrak kering. Analisis kuantitatif dilakukan menggunakan GC-MS. Komposisi dan kuantifikasi asam lemak Xylaria sp. DAP KRI-5 adalah asam linoleat (41,177%; 112,24 mg/L); asam palmitat (25,114%; 68,45 mg/L); asam oleat (14.198%; 38,70 mg/L);  asam stearat (6,575%; 17,2 mg/mL); dan asam palmitoleat 2,165%; 5,90 mg/mL). Persentase asam lemak jenuh dan tidak jenuh berturut-turut adalah 31,69 and 57,54%. Kesimpulan dari penelitian ini menunjukkan jamur endofit Xylaria sp. DAP KRI-5 berpotensi sebagai sumber asam lemak tidak jenuh. Penelitian ini merupakan laporan pertama dari asam lemak yang diproduksi oleh Xylaria sp. DAP KRI-5. 

scholarly journals Profil Asam Lemak Gonad Lima Spesies Landak Laut (Echinoidea) Dari Pantai Selatan Kabupaten Gunung Kidul Daerah Istimewa Yogyakarta

2014 ◽  
Vol 19 (1) ◽  
Author(s):  
Sri Endang Purnami ◽  
Trijoko ◽  
Raras Toeti Pratiwi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Sea Urchin ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Economic Value ◽  
Acid Methyl Ester ◽  
Acid Methyl

AbstractSea urchin (Echinoidea) is an avertebrate animal whose habitat can be found from the intertidal to shallow subtidal areas. Sea urchin has a very important role in reef ecology, especially in intertidal and subtidal areas. Sea urchin gonad also can be consumed and has high economic value. The aim of this study was to determine the profile of fatty acid Sea urchin gonad in South Coral in Gunung Kidul (Daerah Istimewa Yogokarta) Gonad samples were taken from two sampling locations, those were four species from Sepanjang beach and one species from Wediombo coast. Fat was separated from the gonad using the method of Blight and Dyer (1959) and fatty acid methyl ester were prepared by direct transesterification reaction according to Morisson and Smith’s method (1964). Fatty acid methyl ester were separated and analysed by gas chromatography. The result showed that there are 10 types of fatty acid found in sea urchin gonad belonging to saturated and unsaturated fatty acid both MUFA (monounsaturated fatty acid) dan PUFA (polyunsaturated fatty acid). In all samples. The level of saturated fatty acids is higher than the unsaturated fatty acids, especially myristic (C14:0) and palmitic acid (C16:0).Keywords: Fatty acid, Sea Uechin, South Coral Gunung Kidul Daerah Istimewa YogyakartaAbstrakLandak laut (Echinodea) merupakan hewan avertebrata yang banyak dijumpai pada daerah pasang surut yang berbatu dan berpasir. Landak laut memiliki peranan yang sangat penting pada ekologi karang terutama di daerah pasang surut, selain itu gonadnya juga dapat dikonsumsi dan memiliki nilai ekonomi yang tinggi. Tujuan dari penelitian ini mengetahui keragaman spesies dan komposisi asam lemak gonad Landak laut yang ada di Pantai Selatan Kabupaten Gunung Kidul. Sample gonad diambil dari 2 lokasi sampling yaitu empat jenis dari Pantai Sepanjang dan satu jenis dari Pantai Wediombo. Lemak dipisahkan dari gonad menggunakan metode Blight and Dyer (1959) dan dimetilasi melalui metode Morison and Smith (1964). Kadar asam lemak gonad kemudian dianalisa dengan GC. Hasil analisa asam lemak sampel gonad ditemukan Asam lemak jenuh yang meliputi asam kaprilat (C8:0), asam miristat (C14:0), asam palmitat (C16:0), asam stearat (C18:0) dan asam arakhidat (C20:0). Asam lemak tidak jenuh antara lain asam oleat (C18:1n-9), asam palmitoleat (C16:1n-7), linoleat (C18:2n-8) dan asam eicosapentaenoat (C20: 5n-3). Hasil analisa menunjukkan bahwa kadar asam lemak jenuh lebih tinggi dibanding asam lemak tidak jenuh terutama asam miristat sebesar 27,20% dan palmitat 24,44% sedangkan asam lemak tak jenuh yang tinggi adalah asam Eicosapentaenoat sebesar 14,83%, keduanya ditemukan pada Colobocentrotus sp.2. Jenis Landak laut di Pantai Selatan Kabupaten Gunung Kidul sangat beragam sedangkan jenis asam lemak yang terkandung pada lima sampel gonadnya sama tetapi berbeda kadarnya.Kata kunci: Asam lemak, landak laut, karang Gunung Kidul Daerah Istimewa Yogyakarta

Effect of Fatty Acid Methyl Ester on Fuel-Injector Wear Characteristics

2020 ◽  
Vol 14 (3) ◽  
pp. 327-339
Author(s):  
M. Jamshaid ◽  
H. H. Masjuki ◽  
M. A. Kalam ◽  
N. W. M. Zulkifli ◽  
A. Arslan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Palm Oil ◽  
Methyl Esters ◽  
Acid Methyl Ester ◽  
Fuel Injector ◽  
Wear Characteristics ◽  
Acid Methyl

This paper presents the experimental results carried out to evaluate the fatty acid methyl ester (FAME) obtained from cotton-seed oil and palm oil on fuel-injector wear characteristics. The cottonseed oil methyl ester (COME) and palm oil methyl ester (POME) were produced in the laboratory using alkaline transesterification. Gas chromatography based on 'BS EN 14103:2011' standard was used to analyze the percentage of fatty acids in COME and POME. The physicochemical properties of the two methyl esters were measured based on ASTM and EN standards. Various unique blends using cottonseed–palm oil methyl ester (CPME) were tested. Thirteen (13) different types of fuel blends were prepared from COME, POME, and petroleum diesel fuel (DF100). The wear and lubricity characteristics were measured using a high-frequency reciprocating rig (HFRR) based on ASTM D6079 standard. The worn surfaces of the specimen plates were evaluated by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). The COME100, POME100, and CPME100 showed excellent lubricity properties for the fuel injector in terms of lower COF and wear coefficient when compared with DF100. COME100, POME100 and CPME100 showed lower average COF compared to DF100 by 16.9%, 13.9% and 16.1%, respectively. This may be due to the presence of unsaturated fatty acids in the methyl esters composition. Consequently, the fatty acid methyl esters can be used to reduce the friction and wear of the fuel injectors due to the improvement in the tribological properties of the fuel.

scholarly journals The Role of Ionic Liquid Interaction in the Separation of Fatty Acid Methyl Esters—Polyunsaturated Geometric Isomers in GC–MS

Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 38
Author(s):  
Emerencia Mező ◽  
Anita Bufa ◽  
Csilla Páger ◽  
Viktória Poór ◽  
Tamás Marosvölgyi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ionic Liquid ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Unsaturated Fatty Acids ◽  
Limit Of Detection ◽  
Practical Importance ◽  
Acid Methyl Ester ◽  
Geometric Isomers ◽  
Acid Methyl

Knowledge of the type and level of saturated and unsaturated fatty acids in food and clinical matrices is of practical importance, but the wide variety of fatty acids makes analyses very complex. The discrimination of the geometric isomers of fatty acid needs proper and effective separation conditions. The efficiency of three different stationary phases was evaluated by GC–MS methods in the separation of fatty acids in their methyl ester forms. Significant differences were observed in the efficiencies of polysiloxane-based (non-polar HP-5MS and medium/high polarity DB-225MS) and ionic liquid-based (SLB-IL111) columns. Baseline separation of the geometric isomers of linoleic acid methyl ester was obtained by the extremely polar SLB-IL111 column, showing a preference over the other two columns. The optimization of the experimental conditions (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) showed the separation power of the ionic liquid interaction in the analyses by using short (25–30 m long) columns. By deducting the general principles of the interaction, predictions can be made for the separation of other isomers. The results facilitate the precise identification of various types of fatty acids in real samples for nutritional information.

Synthesis and kinetics study of trimethylolpropane fatty acid triester from oleic acid methyl ester as potential biolubricant

2021 ◽  
Author(s):  
Mamdouh S. Elmelawy ◽  
Asmaa El-Meligy ◽  
Hussien A. Mawgoud ◽  
Asmaa S. Morshedy ◽  
Samia A. Hanafy ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Acid Methyl Ester ◽  
Kinetics Study ◽  
Acid Methyl ◽  
Oleic Acid Methyl Ester

Fatty Acids in Tamarindus indica L. Seeds Oil and Antibacterial Activity Assay

2019 ◽  
Vol 811 ◽  
pp. 40-46 ◽  
Author(s):  
Sutrisno ◽  
Rini Retnosari ◽  
Siti Marfu'ah ◽  
Fauziatul Fajaroh
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Acid Methyl Ester ◽  
Esterification Reaction ◽  
Tamarindus Indica ◽  
Acid Methyl

The research was conducted to investigate the oil and fatty acids contained in Tamarindus indica Linn (tamarind) seeds oil. Tamarind seeds oil was isolated by extraction and fractionation. The fatty acids content in oil were identified as fatty acid methyl ester. Fatty acid methyl ester was produced from trans-esterification reaction of tamarind seed oil with methanol and boron trifluoride catalyst (MeOH/BF3). Identification of fatty acid methyl ester was carried out by gas chromatograph mass spectrometry (GC-MS). The fatty acids in the Tamarindus indica seed oil are saturated and unsaturated fatty acids. The saturated fatty acids are octanoic (12.66%), decanoic (1.68%), dodecanoic (25.18%), tetradecanoic (5.17-7.83%), hexadecanoic (9.90-16.06%), octadecanoic (3.82-4.80%), eicosanoic (0.39-1.55%), docosanoic (1.00-2.01%), and tetracosanoic (1.92-4.54%) acids. The unsaturated fatty acids are 11-octadecenoic (19.93%), 11-eicosenoic (0.76-1.03%), 9,12-octadecadienoic (21.91-38.68%), and 9-octadecenoic (17.76%) acids. The physical and antibacterial properties of the seed oil are also reported.

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