scholarly journals Exploration of IAA and HCN Producing Rhizobacteria from Cassava (Manihot esculenta Crantz) Rhizosphere

2020 ◽  
Vol 16 (2) ◽  
pp. 71
Author(s):  
Suri Raihan Safriani ◽  
Lenni Fitri ◽  
Yulia Sari Ismail
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Hypersensitive Reaction ◽  
Manihot Esculenta Crantz ◽  
Ability Test ◽  
Alternative Approach ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

<p>Plant growth promoting rhizobacteria (PGPR) are a group of beneficial bacteria that live in rhizosphere. These bacteria can promote plant growth through several mechanisms, such as the ones produce indole-3-acetic acid (IAA) hormone and hydrogen cyanide (HCN), and act as biocontrol agents. The use of PGPR to promote plant growth has been known to be an environmentally friendly alternative approach. The aim of this study was to explore IAA and HCN producing rhizobacteria from cassava rhizosphere soil and identify the bacteria based on morphological and biochemical characters, hypersensitive reaction test, and the ability test to produce IAA and HCN. The results showed nine bacterial isolates suspected as Micrococcus sp. (six isolates), Neisseria sp. (two isolates), and Bacillus sp. (one isolate). All isolates were able to produce IAA in the concentration range of 50,63–135,00 µg/ml and 232,3–333,9 µg/ml at incubation time of 2 and 4 days, respectively. All isolates were able to produce HCN. In addition, the isolates did not show hypersensitivity reactions. Further study is needed to assess the isolate application for promoting plant growth as well as a biocontrol agent of plant pathogen.</p>

scholarly journals Rhizobacteria Associated with a Native Solanaceae Promote Plant Growth and Decrease the Effects of Fusariumoxysporum in Tomato

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 579
Author(s):  
Carmen Sanjuana Delgado-Ramírez ◽  
Rufina Hernández-Martínez ◽  
Edgardo Sepúlveda
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Positive Effects ◽  
Alternative Approach ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Plant growth-promoting rhizobacteria are often utilized to improve crop health and productivity. Nevertheless, their positive effects can be hindered if they fail to withstand the environmental and ecological conditions of the regions where they are applied. An alternative approach to circumvent this problem is a tailored selection of bacteria for specific agricultural systems. In this work, we evaluated the plant growth promoting and pathogen inhibition activity of rhizobacteria obtained from the rhizosphere of Mariola (Solanum hindsianum), an endemic shrub from Baja California. Eight strains were capable of inhibiting Fusarium oxysporum in vitro, and thirteen strains were found to possess three or more plant-growth-promotion traits. Molecular identification of these strains, using 16 s rRNA partial sequences, identified them as belonging to the genera Arthrobacter, Bacillus, Paenibacillus, Pseudomonas, and Streptomyces. Finally, the effect of selected plant growth-promoting rhizobacteria (PGPR) strains on the growth and suppression of Fusarium wilt in tomato was evaluated. Results showed that these strains improved tomato plants growth under greenhouse conditions and reduced Fusarium wilt effects, as reflected in several variables such as length and weight of roots and stem. This work highlights the potential of native plants related to regionally important crops as a valuable source of beneficial bacteria.

A Study on the Farmers’ Awareness and Acceptance of Biofertilizers in Kottayam District

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 425-431
Author(s):  
Subin Thomas ◽  
Dr. M. Nandhini
Keyword(s):  
Organic Matter ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nutrient Cycle ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Structured Questionnaire ◽  
Area Data

Biofertilizers are fertilizers containing microorganisms that promote plant growth by improving the supply of nutrients to the host plant. The supply of nutrients is improved naturally by nitrogen fixation and solubilizing phosphorus. The living microorganisms in biofertilizers help in building organic matter in the soil and restoring the natural nutrient cycle. Biofertilizers can be grouped into Nitrogen-fixing biofertilizers, Phosphorous-solubilizing biofertilizers, Phosphorous-mobilizing biofertilizers, Biofertilizers for micro nutrients and Plant growth promoting rhizobacteria. This study conducted in Kottayam district was intended to identify the awareness and acceptance of biofertilizers among the farmers of the area. Data have been collected from 120 farmers by direct interviews with structured questionnaire.

Rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce

1996 ◽  
Vol 42 (3) ◽  
pp. 279-283 ◽  
Author(s):  
T. C. Noel ◽  
C. Sheng ◽  
C. K. Yost ◽  
R. P. Pharis ◽  
M. F. Hynes
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Direct Growth ◽  
Early Seedling ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus cv. Westar) and lettuce (Lactuca saliva cv. Grand Rapids) was significantly promoted by inoculation of seeds with certain strains of Rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. Auxotrophic Rhizobium mutants requiring tryptophan or adenosine (precursors for indole-3-acetic acid and cytokinin synthesis, respectively) did not promote growth to the extent of the parent strain. The findings of this study demonstrate a new facet of the Rhizobium–plant relationship and that Rhizobium leguminosarum can be considered a plant growth-promoting rhizobacterium (PGPR).Key words: Rhizobium, plant growth-promoting rhizobacteria, PGPR, indole-3-acetic acid, cytokinin, roots, auxotrophic mutants.

scholarly journals In vitro Screening and Optimization of IAA Production from Plant Growth Promoting Rhizobacteria Burkholderia cepacia UPMB3

2018 ◽  
Vol 28 (1) ◽  
pp. 25-34
Author(s):  
Waheeda Parvin ◽  
Quazi Shirin Akhter Jahan ◽  
Md Mahbubur Rahman ◽  
Mui Yun Wong
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Leaf Length ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Layer Chromatography

Burkholderia cepacia UPMB3 is an important plant growth promoting rhizobacteria isolated from oil palm rhizosphere which is considered to promote plant growth directly or indirectly. The IAA was extracted, purified, detected and confirmed by thin layer chromatography analyses f r om t h e strain UPMB3 of B. cepacia. Rf value was compared with the authentic IAA. Maximum 50 μg/ml IAA was produced in the medium supplemented with 4 mg/ml L-tryptophan, under shaken conditions at 150 rpm in seven days incubation at pH 7. The bacterial extract significantly influenced the growth of oil palm seedlings producing shoot, root, leaf and leaf length compared to control.Plant Tissue Cult. & Biotech. 28(1): 25-34, 2018 (June)

scholarly journals Extraction of Indole-3-acetic Acid from Plant Growth Promoting Rhizobacteria of Bamboo Rhizosphere and Its Effect on Biosynthesis of Chlorophyll in Bamboo Seedlings

2020 ◽  
Author(s):  
Janardan Lamichhane ◽  
Bishnu Maya K.C. ◽  
Dhurva Prasad Gauchan ◽  
Sanjay Nath Khanal ◽  
Sharmila Chimouriya
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Biochemical Analysis ◽  
Chlorophyll Biosynthesis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Agitation Rate ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Background: Indole-3-acetic acid (IAA), a principal phytohormone, controls several crucial physiological processes of plants. It ameliorates plant growth by stimulating cell elongation, root initiation, seed germination and seedling growth. Alteration of IAA level by plant growth promoting rhizobacteria leads to varied impacts on plant growth and development. Methods: Soil samples were collected from bamboo (Bambusa tulda, B. nutans subsp. cupulata, B. balcooa and Dendrocalamus strictus) rhizosphere. Altogether five bacterial isolates were screened by serial dilution method and subjected to biochemical analysis. The isolate BUX1 with high IAA production capacity was optimized for IAA production. IAA was partially purified and quantified from the bacterial extract by thin layer chromatography (TLC). The influence of extracted bacterial IAA on chlorophyll biosynthesis in bamboo seedlings of B. tulda was compared with uninoculated control plants. Results: Biochemical analysis revealed that all the isolates belonged to genus Bacillus which were found capable of producing IAA. During optimization, BUX1 isolate produced 99.13 µg ml-1 of IAA at 37°C, pH 7, 3 mg l-1 concentration of L-tryptophan and 150 rpm agitation rate after 192 hour of incubation. The Rf value of the bacterial IAA during TLC was identical to that of standard IAA (0.425) indicating that IAA was present in crude extract of Bacillus (BUX1). The influence of bacterial IAA on chlorophyll biosynthesis in bamboo seedlings was significant in comparison to uninoculated plants. Therefore, this isolate could be a prospective candidate to be employed as biofertilizer.

scholarly journals ENDOPHYTIC BACTERIA USED AS BIOINOCULANTS IN MICROPROPAGATED BANANA SEEDLINGS

2017 ◽  
Vol 39 (2) ◽  
Author(s):  
GLEIKA LARISSE OLIVEIRA DORASIO DE SOUZA ◽  
DEIVISSON FERREIRA DA SILVA ◽  
SILVIA NIETSCHE ◽  
ADELICA APARECIDA XAVIER ◽  
MARLON CRISTIAN TOLEDO PEREIRA
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Plant Growth Promoting Bacteria ◽  
Bacillus Sp ◽  
Microbial Inoculants ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT The use of bio-fertilizers and microbial inoculants that promote plant growth and increased yield has been accepted as an alternative to reduce the use of chemical fertilizers. The objective of the present study was to evaluate the effect of plant growth promoting bacteria inoculation on growth and quality of micropropagated banana seedlings ‘Prata Anã’. The experimental design was completely randomized, with four replications and the treatments consisted of 24 isolates of endophytic bacteria. The isolates EB-50 (Bacillus sp.) and EB-133 (Bacillus amyloliquefaciens) characterized as diazotrophic, the EB-51 (Bacillus pumilus) indicate for inorganic phosphate solubilization and EB-55(Bacillus subtilis) and EB-40 (Bacillus sp.) indole-3-acetic acid producers have provided significant increases for length, pseudostem diameter, fresh masses and dry masses in ‘Prata Anã’ micropropagated banana seedlings.

A novel procedure for rapid isolation of plant growth promoting pseudomonads

1995 ◽  
Vol 41 (6) ◽  
pp. 533-536 ◽  
Author(s):  
Bernard R. Glick ◽  
Damir M. Karaturovíc ◽  
Peter C. Newell
Keyword(s):  
Plant Growth ◽  
Soil Bacteria ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacterial Strains ◽  
Seedling Root ◽  
N Source ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

A rapid and novel procedure for the isolation of plant growth promoting rhizobacteria (PGPR) is described. This method entails screening soil bacteria for the ability to utilize the compound 1-aminocyclopropane-1-carboxylate (ACC) as a sole N source, a trait that is a consequence of the presence of the activity of the enzyme ACC deaminase. This trait appears to be limited to soil bacteria that are also capable of stimulating plant growth. Seven different soil samples from two geographically disparate locations were found to contain pseudomonads that were able to to utilize ACC as a N source. Each of the seven strains was shown, by the ability of the bacterium to promote canola seedling root elongation under gnotobiotic conditions, to be a PGPR. The method described here may be used to replace the otherwise slow and tedious process of testing individual bacterial strains for their ability to promote plant growth, thereby significantly speeding up the process of finding new PGPR.Key words: plant growth promoting rhizobacteria, PGPR, 1-aminocyclopropane-1-carboxylate, ACC, ACC deaminase, bacterial fertilizer, soil bacteria.

Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria

2007 ◽  
Vol 53 (2) ◽  
pp. 159-167 ◽  
Author(s):  
J.W. Kloepper ◽  
A. Gutiérrez-Estrada ◽  
J.A. McInroy
Keyword(s):  
Plant Growth ◽  
Induced Resistance ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Leachate Quality ◽  
Shoot Mass

For several years, we have noticed that plant growth-promoting rhizobacteria (PGPR), which consistently promote plant growth in greenhouse tests during spring, summer, and fall, fail to elicit plant growth promotion during the midwinter under ambient light conditions. This report tests the hypothesis that photoperiod regulates elicitation of growth promotion and induced systemic resistance (ISR) by PGPR. A commercially available formulation of PGPR strains Bacillus subtilis GB03 and Bacillus amyloliquefaciens IN937a (BioYield®) was used to grow tomato and pepper transplants under short-day (8 h of light) (SD) and long-day (12 h of light) (LD) conditions. Results of many experiments indicated that under LD conditions, BioYield consistently elicited significant increases in root and shoot mass as well as in several parameters of root architecture. However, under SD conditions, such increases were not elicited. Differential root colonization of plants grown under LD and SD conditions and changes in leachate quality partially account for these results. BioYield elicited ISR in tomato and pepper under both LD and SD conditions, indicating that although growth promotion was not elicited under SD conditions, induced resistance was. Overall, the results indicate that PGPR-mediated growth promotion is regulated by photoperiod, while ISR is not.

scholarly journals Plant growth-promoting rhizobacteria associated to candelilla rhizosphere (Euphorbia antisyphilitica) and its effects on Arabidopsis thaliana seedlings

2021 ◽  
Vol 49 (2) ◽  
pp. 12294
Author(s):  
Maria T. SALAZAR-RAMÍREZ ◽  
Jorge SÁENZ-MATA ◽  
Pablo PRECIADO-RANGEL ◽  
Manuel FORTIS-HERNÁNDEZ ◽  
Edgar O. RUEDA-PUENTE ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth Promoters ◽  
Biochemical Tests ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

In the communities of Sierra Mojada and Viesca, Coahuila, Mexico of Coahuila desert, two rhizosphere samplings of candelilla (Euphorbia antisyphilitica Zucc) were collected to isolate, characterize, and identifying plant growth-promoting rhizobacteria (PGPR); 165 rhizobacteria were tested in vitro with Arabidopsis thaliana seedlings to evaluate their potential as plant growth promoters, and obtaining 21 strains with best results in the variables of the number of secondary roots and fresh weight concerning the uninoculated control. Their salinity tolerance was evaluated at concentrations from 0.85 M, 1.7 M and 2.55 M of NaCl. Biochemical tests were accomplishing such as siderophores production, phosphates solubilization, production of Indole-3-acetic acid (IAA), and the activity of the ACC deaminase enzyme. The results obtained from 21 strains selected, high activities were obtained in organic substances like a siderophores since they developed a translucent orange halo around their growth; four rhizobacteria developed a clear halo around the bacterial growth with a thickness between 1.487 mm ± 0.667 mm and 5.267 mm ± 0.704 mm in phosphates solubilization; in the production of Indole-3-acetic acid (IAA), the bacterial strains showed the presence of this phytohormone, with values ​​from 4.444 μg mL-1 to 19.286 μg mL-1; and according to the activity of the ACC deaminase enzyme, values ​​from 0.424 to 1.306 µmol α-KB/h/mg Pr were showed. 16S rRNA sequencing was carried out and genus identified were Bacillus, Staphylococcus, Acinetobacter, Cronobacter and Siccibacter. The results obtained show the potential of the isolated rhizobacteria as growth promoters and the increase in the biomass of the Arabidopsis thaliana seedlings is evident. This is a first indication to proceed to carry out tests in different phenological stages in crops of agricultural importance.

Sign in / Sign up

Export Citation Format

Share Document