scholarly journals Development of Transgenic Sugarcane Resistant to Stem Borer by Transforming cry1Ab-cry1Ac Fusion Gene through Agrobacterium tumefaciens Transformation Method

2021 ◽  
Vol 17 (1) ◽  
pp. 11
Author(s):  
Deden Sukmadjaja ◽  
Sri Koerniati ◽  
Auliya Lukman
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Molecular Analysis ◽  
Transformation Efficiency ◽  
Insect Pests ◽  
Fusion Gene ◽  
Stem Borer ◽  
Transformation Method ◽  
High Yield ◽  
Research Activities ◽  
Sugarcane Genome

<p>Bulu Lawang (BL) is a sugarcane variety preferred by farmers in Indonesia due to its high yield, but this cultivar is susceptible to shoot and stem borer insect pests. Genetic engineering using cry1Ab and cry1Ac fusion gene is an effort to generate BL varieties resistant to the insect pests. This study aimed to 1) transform T-DNA containing cry1Ab-cry1Ac fusion gene into sugarcane genome by using Agrobacterium tumefaciens method, 2) obtain selection media composition of callus transformants, and 3) obtain transformation efficiency comparison of A. tumefaciens strains EHA105 and GV3101. The research was conducted at the Laboratory of Cell and Tissue Biology, the Laboratory of Molecular Biology, and the greenhouse of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor from March to August 2019. Research activities consisted of four parts namely 1) callus induction and Agrobacterium culture preparation containing pCambia5300-cry1Ab-cry1Ac ///// pCambia-5300_OaRbcS-prom-cTP-cry1Ab-cry1Ac plasmid, 2) callus incubation in cocultivation and resting media, 3) selection and differentiation of shoots on regeneration media, and 4) molecular analysis using PCR method. Results showed that the composition of media, both for selection and regeneration processes of putative plant transformants, was the key to the success of this experiment. A. tumefaciens strain EHA105 resulted in higher transformation efficiency (11.1%) compared to that of strain GV3101 (9.0%). Molecular analysis showed that cryIAb-cryIAc fusion gene was successfully inserted into the sugarcane genome suggesting that the transgenic plant containing cry1Ab-cry1Ac fusion gene was obtained. The putative transgenic plants need further assay through bioassay tests to verify its resistance phenotype to the insect pests.</p>

scholarly journals Relative Abundance and Field Efficacy of Insecticides and Bio-control Agents Against Stem Borer Species in Rice (Oryza sativa L.)

2021 ◽  
Author(s):  
T. Sharmitha ◽  
C. Gailce Leo Justin ◽  
S. Sheeba Joyce Roseleen
Keyword(s):  
Field Experiments ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Stem Borer ◽  
Field Trials ◽  
Control Agent ◽  
High Yield ◽  
Seasonal Incidence ◽  
Yellow Stem Borer ◽  
Highly Effective

Background: In India, out of the total loss incurred by different insect pests of paddy, 25 to 30 per cent damage is done by stem borer alone. Many conventional insecticides though have been evaluated against stem borers, yet, most of the chemicals have failed to provide adequate control. Hence, the present study aimed to evaluate the efficacy of some newer molecules of insecticides and bio-control agents.Methods: Field trials were conducted to record the seasonal incidence and to evaluate the efficacy of insecticides and bio-control agents against the stem borer species in rice during Kharif and Rabi. Result: Field experiments revealed that during Kharif 2018, chlorantraniliprole 18.5 SC was highly effective with 92.98 and 91.24 per cent reduction of stem borer infestation over control at vegetative and reproductive stages, respectively and recorded a high yield of 5720 kg/ha followed by spinetoram 11.7 SC with 83.16 and 80.82 per cent reduction of stem borer over control at vegetative and reproductive stages, respectively. During Rabi 2019, spinetoram 11.7 SC was highly effective with 92.36 and 93.73 per cent reduction of stem borer infestation over control at vegetative and reproductive stages respectively and recorded a high yield of 4570 kg/ ha, followed by chlorantraniliprole 18.5 SC with 84.60 and 91.48 per cent reduction of stem borer at vegetative and reproductive stages, respectively. The difference in the efficacy of insecticides during Khaif, 2018 and Rabi, 2019, might be due to shift in the species occurrence of stem borer, with a dominant occurrence of yellow stem borer (80.61%) in Kharif 2018 and pink stem borer (70.00%) in Rabi, 2019. The bio-control agent Trichogramma japonicum exerted minimum reduction of stem borer with a low grain yield of 3946.6 kg/ha.

scholarly journals Agrobacterium tumefaciens-MEDIATED IN-PLANTA TRANSFORMATION OF INDONESIAN MAIZE USING pIG121Hm-Cs PLASMID CONTAINING nptII AND hpt GENES

2017 ◽  
Vol 17 (2) ◽  
pp. 49
Author(s):  
Edy Listanto ◽  
Eny Ida Riyanti ◽  
Sustiprijatno Sustiprijatno
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Engineering ◽  
Molecular Analysis ◽  
Low Cost ◽  
Transformation Method ◽  
Pcr Analysis ◽  
In Planta ◽  
Simple Method ◽  
Maize Genetic ◽  
Selection Medium

<p class="abstrakInggris"><span>Maize (<em>Zea mays</em> L.) productivity in Indonesia is challenged to be increased using genetic engineering. Recent advances in <em>Agrobacterium tumefaciens</em>-mediated <em>in-planta</em> transforma-tion makes it possible<strong> </strong>to<strong> </strong>transform maize with low cost, and simple method. This study aimed to confirm pIG121Hm-<em>Cs</em> plasmid in <em>A. tumefaciens</em>, and to estimate the efficiency level of<strong> </strong> <em>A<strong>. </strong>tumefaciens</em>-mediated <em>in-planta</em> transformation of Indonesian maize by<strong> </strong>using pIG121Hm-<em>Cs</em> plasmid containing <em>npt</em>II and<strong> </strong><em>hpt</em><strong> </strong>genes. A series of studies were conducted including confirmation of gene construct of pIG121Hm-<em>Cs</em> plasmid in <em>A. tumefaciens</em>, transformation of four maize lines through <em>A. tumefaciens</em>-mediated <em>in-planta</em> technique, acclimatization of transformant plants and molecular analysis of selected plants using polymerase chain reaction (PCR). The pIG121Hm-<em>Cs</em> plasmid was confirmed via PCR analysis using specific primers of <em>npt</em>II and <em>hpt</em> genes and resulted 700 bp and 500 bp for fragments of <em>npt</em>II and <em>hpt</em>, respectively. After selection, acclimatization and molecular analysis steps, the efficiency levels of transformation of four maize lines were low, ranging from 3.8% to 12.8%. The level of transformation efficiency of ST-27 line was the highest accounting for 12.8% of 45 planted embryos on selection medium based on PCR analysis using specific primer for <em>npt</em>II gene. Overall, <em>A. tumefaciens</em>-mediated <em>in planta</em> transformation on maize floral pistil in this study proved to be successful and rapid. Therefore, this enhanced transformation method will be beneficial for Indonesian maize genetic engineering.</span></p>

Efficacy of some insecticide modules against major insect pests and spider population of rice, Oryza sativa L.

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Atanu Seni ◽  
Bhimasen Naik
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Untreated Control ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Gall Midge ◽  
Stem Borer ◽  
The Other ◽  
The Third ◽  
Treated Plot

Experiments were carried out to assess some insecticide modules against major insect pests of rice. Each module consists of a basal application of carbofuran 3G @ 1 kg a.i ha-1 at 20 DAT and Rynaxypyr 20 SC @ 30 g a.i ha-1 at 45 DAT except untreated control. All modules differ with each other only in third treatment which was applied in 65 DAT. The third treatment includes: Imidacloprid 17.8 SL @ 27 g a.i ha-1, Pymetrozine 50 WG @ 150 g a.i ha-1, Triflumezopyrim 106 SC @ 27 g a.i ha-1, Buprofezin 25 SC @ 250 g a.i ha-1; Glamore (Imidacloprid 40+Ethiprole 40% w/w) 80 WG @ 100 g a.i. ha-1, Thiacloprid 24 SC @ 60 g a.i ha-1, Azadirachtin 0.03 EC @ 8 g a.i ha-1, Dinotefuran 20 SG@ 40 g a.i ha-1 and untreated control. All the treated plots recorded significantly lower percent of dead heart, white ear- head caused by stem borer and silver shoot caused by gall midge. Module with Pymetrozine 50 WG @ 150 g a.i ha-1 treated plot recorded significantly higher per cent reduction of plant hoppers (>80% over untreated control) and produced higher grain yield (50.75 qha-1) than the other modules. Among the different treated modules the maximum number of spiders was found in Azadirachtin 0.03 EC @ 8 g a.i ha-1 treated module plot followed by other treatments.

scholarly journals Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

2021 ◽  
Vol 7 (7) ◽  
pp. 520
Author(s):  
Jianmin Fu ◽  
Nohelli E. Brockman ◽  
Brian L. Wickes
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Yeast Cell ◽  
Transformation Efficiency ◽  
Transformation Frequency ◽  
Yeast Cells ◽  
Agar Concentration ◽  
Genetic Tool ◽  
Number Of Factors ◽  
Cryptococcus Spp ◽  
Transformation Systems

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar concentration was found to have a significant effect on the transformant number as transformants increased with agar concentration across all four serotypes. When infection time points were tested, higher agar concentrations were found to result in an earlier transfer of the Ti-plasmid to the yeast cell, with the earliest transformant appearing two h after A. tumefaciens contact with yeast cells. These results demonstrate that A. tumefaciens transformation efficiency can be affected by a variety of factors and continued investigation of these factors can lead to improvements in specific A. tumefaciens/fungus transformation systems.

scholarly journals Appraisal of cowpea cropping systems and farmers’ perceptions of production constraints and preferences in the dry savannah areas of Nigeria

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Saba Baba Mohammed ◽  
Daniel Kwadjo Dzidzienyo ◽  
Muhammad Lawan Umar ◽  
Mohammad Faguji Ishiyaku ◽  
Pangirayi Bernard Tongoona ◽  
...  
Keyword(s):  
Plant Density ◽  
Insect Pests ◽  
Cropping Systems ◽  
Pairwise Comparison ◽  
High Yield ◽  
Crop Failure ◽  
Production Constraints ◽  
Cowpea Varieties ◽  
Breeding Programmes ◽  
Sole Cropping

Abstract Background Low plant density and wide intra-plant spacing in traditional cowpea cropping systems are among the factors responsible for low yield on farmers’ fields. Sole cropping and improved intercropping systems have been advocated in the last few years to increase yield in the dry savannah areas of Nigeria. This study investigated the level of adoption of high yielding cowpea cropping systems including factors that influenced their use and farmers’ perceived production constraints and preferences. A total of 420 farmers across 36 villages of northern Nigeria were interviewed, and data collected was analyzed using descriptive statistics to appraise farmers predominant cowpea cropping systems and factors that determine the use of sole versus intercropping were identified with the aid of binary logit regression. Furthermore, pairwise comparison ranking was deployed to understand farmers’ view of cowpea production constraints and preferred traits. Results The results revealed that, many of the farmers (42%) still grow cowpeas in the traditional intercropping and a good number (25%) cultivate the crop as a sole crop, while 23% had fields of cowpeas in both sole and intercropping systems. Farmers reported the incidence of high insect pests, limited access to land, desire to have multiple benefits, and assurance in the event of crop failure as reasons for preference for intercropping over sole planting. The pairwise comparison ranking of constraints and preferences revealed insect pests, Striga, drought and poor access to fertilizers as major constraints to increased productivity. Many farmers indicated high yield as the most preferred trait. Conclusions Findings indicate a need for increased education and training of cowpea farmers on the importance of growing cowpeas in sole cropping and or improved intercropping systems. Genetic improvement efforts should focus on developing cowpea varieties that address farmers production constraints and reflect the diversity of consumers’ preferences for the crop. Hence, breeding for resistance to insect pests and high yield is recommended as an important priority of cowpea breeding programmes in the region.

Insect Pests of Shorea robusta Gaertn. f. : An Update

2015 ◽  
Vol 38 (4) ◽  
pp. 313-322
Author(s):  
N. Roychoudhury
Keyword(s):  
Insect Pests ◽  
Stem Borer ◽  
North India ◽  
White Grubs ◽  
Insect Fauna ◽  
Living Tree ◽  
Shorea Robusta ◽  
Damage Seed ◽  
Indian Origin ◽  
Lepidoptera Noctuidae

Shorea robusta Gaertn. f. (Dipterocarpaceae), commonly known as sal, is one of the most important timbers of India both ecologically and economically. It is of Indian origin, widely distributed in central and north India and constitutes an important ecosystem, which provides cool and calm environment rich in biodiversity. This tree species has a highest number of insect fauna among the forest trees. Of about 346 insects recorded on sal, about 155 species of insects belonging to the order Lepidoptera (105), Coleoptera (31), Thysanoptera (9), Hemiptera (4), Orthoptera (4), Ephemeroptera (1) and Isoptera (1) are associated with living tree. The major insect pests of sal are, ‘seed and seedling borer’ Pammene theristis Meyrick (Lepidoptera: Eucosmidae), feeding on seed while they are on the tree; Sitophilus rugicollis Casey (Coleoptera : Curculionidae), damage seed either on the forest floor or during storage; white grubs such as Holotrichia spp. (Coleoptera : Scarabaeidae), which feed on the root system of the plants in nurseries and beetles feed on the foliage; defoliators such as Caviria sericea Moore (Lepidoptera : Lymantriidae), Lymantria mathura Moore (Lepidoptera : Lymantriidae), Paectes subapicalis Walker (Lepidoptera : Noctuidae), Trabala vishnou Lefebvre (Lepidoptera : Lasiocampidae), which feed on the leaves; stem borer such as Hoplocerambyx spinicornis Newman (Coleoptera : Cerambycidae) and sap sucker, such as Drosicha stebbingi Green (Hemiptera : Coccidae). Termites, such as Coptotermes curvignathus Holmgren (Isoptera : Rhinotermitidae) and timber borers, such as Sinoxylon anale and S. crassum (Coleoptera : Bostrychidae), damage sal timber in dying, felled or stored logs in depots.

scholarly journals Aspectos Bionômicos de Spodoptera eridania (Cramer): Uma Praga em Expansão na Cultura da Soja na Região do Cerrado Brasileiro

2014 ◽  
Vol 7 (2) ◽  
pp. 75-80 ◽  
Author(s):  
Bruno Henrique Sardinha de Souza ◽  
Eduardo Neves Costa ◽  
Anderson Gonçalves da Silva ◽  
Arlindo Leal Boiça Júnior
Keyword(s):  
International Trade ◽  
Glycine Max ◽  
Insect Pests ◽  
High Yield ◽  
Spodoptera Eridania ◽  
Soybean Crop ◽  
Glycine Max L ◽  
Midwest Region ◽  
Soybean Plants ◽  
Economical Losses

A soja, Glycine max (L.) Merril, é uma das culturas de maior importância econômica para o Brasil, considerada uma commodity nacional devido à sua alta produtividade e participação nas exportações no mercado internacional. Dentre os insetos-pragas que causam danos para essa cultura, nos últimos anos agrícolas têm merecido destaque as lagartas de Spodoptera eridania (Cramer), as quais podem se alimentar tanto de folhas quanto das vagens das plantas de soja, causando prejuízos econômicos para os sojicultores, principalmente nas áreas do Cerrado localizadas na região Centro-Oeste do país. O objetivo da presente revisão é disponibilizar informações sobre os aspectos bionômicos de S. eridania, a fim de dar subsídios para futuras pesquisas sobre o manejo dessa praga.Bionomic Aspects of Spodoptera eridania (Cramer): A Pest in Expansion on Soybean Crop in the Region of Brazilian CerradoAbstract. Soybean, Glycine max (L.) Merril, represents one of the major economically important crops to Brazil, and is considered a national commodity because of its high yield and participation in international trade exportations. Among the insect pests that cause damage to this crop, Spodoptera eridania (Cramer) larvae highlighted in the last agricultural seasons by feeding on leaves and pods of soybean plants, and hence causing economical losses to soybean growers, especially in the Cerrado areas located in the Midwest region of the country. We aimed with this review to provide information about bionomical aspects of S. eridania in order to give subsides for further researches on the management of this pest.

scholarly journals Effectiveness Test of Parasitization by Parasitoid Tricogramma japonicum in Controlling White Rice Stem Borer (Scirphopaga innotata)

2021 ◽  
Vol 2 (1) ◽  
pp. 25-30
Author(s):  
Susilawati Astrodjojo ◽  
Suratman Sudjud ◽  
Sri Soenarsih DAS
Keyword(s):  
Block Design ◽  
Stem Borer ◽  
Plant Age ◽  
High Yield ◽  
Intensity Parameter ◽  
Factor B ◽  
White Rice ◽  
Rice Stem Borer ◽  
Randomized Block Design ◽  
Essential Food

Rice is an essential food crop besides corn and soybeans. The need for rice each year increases along with population growth. One of the pests affecting rice is the white rice stem borer. The present research aimed to test the effectiveness and to examine the interaction between plant age and the number of Tricogramma japonicum parasitoid. The research was carried out at the Agrotechnology Laboratory of the Faculty of Agriculture, Khairun University, Ternate, and the rice fields of Bumi Restu village SP I, East Wasile district. The research design operative was a randomized block design where factor A (A1 = 21 DAS, A2 = 42 DAS, A3 = 56 DAS) was the age of the plant, and factor B (B0 = 0, B1 = 50, B2 = 100, B3 = 150) was the number of eggs of Corcyra cephalonica. Biological control using different amounts of Trichogramma japonicum parasitoid led to different interactions and results for each tested parameter. For the number of egg groups, there was no interaction with plant age, but the treatments which were found effective in suppressing stem borer populations were B2 and B3 (100 and 150). The most effective pias as indicated by damage intensity parameter was B3 (150), which was applied to plants aged 21 DAP. Furthermore, for plant height, different number of parasitoids poses significantly effective results. Percentage unhulled grain of pias B3 (150) causes insignificant damage. For production there is no interaction with plant age, but pias B2 (100) shows high yield. The conclusion is that pias B3 (150) applied to 21 days of plants is very effective in controlling white rice stem borer.

scholarly journals UJI EFEKTIVITAS NEMATODA ENTOMOPATOGEN (RHABDITIDA: Steinernema DAN Heterorhabditis) SEBAGAI MUSUH ALAMI NON-ENDEMIK PENGGEREK BATANG PADI KUNING (Scirpophaga incertulas)

2007 ◽  
Vol 7 (2) ◽  
pp. 71-79
Author(s):  
Chaerani Chaerani ◽  
Bebet Nurbaeti
Keyword(s):  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Larval Mortality ◽  
Damage Threshold ◽  
Stem Borer ◽  
Leaf Sheath ◽  
Scirpophaga Incertulas ◽  
Potted Plants ◽  
Plant Damage ◽  
Rice Stem Borer

Efficacy Tests of Entomopathogenic Nematodes (Rhabditida:  Steinernema dan Heterorhabditis) as Non-endemic Natural Enemies of Yellow Rice stem Borer (Scirpophaga incertulas). Yellow rice stem borer (Scirpophaga incertulas) is a chronic insect pests of irrigated rice and difficult to control.  Entomopathogenic nematodes from the genus Steinernema and Heterorhabditis are promising biological control agents for this pest as their infective juveniles (IJs) are capable of seeking and infecting insect living in moist, cryptic habitat such as galleries created by stem borer larvae. Thirteen indigenous and exotic Steinernema and Heterorhabditis sprayed to rice seedlings in laboratory with nematodes at concentrations of 0.5 or 2.0×104 IJs ml-1 water caused larval mortality between 7–93%.  Further test in greenhouse on nematodes that had >50%  efficacy showed that an indigenous isolate, H. indicus INA H17, was the most effective among the tested nematodes in killing larvae or pupae (78%).  Reduction in plant damage caused by the insects could not be demonstrated as the trials was limited to potted plants. The survival ability of nematodes on rice plants was evaluated by using INA H4 as an example. A low percentage of INA H4 IJs (0.5%) persisted in inner leaf sheath until 7 days post application, while IJs on leaf surface and outer leaf sheath survived only until 2 and 48 hours post application, respectively. Improvement of application strategies including repeated spray, addition of antidessicant and adjusment of spray volume and application at damage threshold or plant critical period are deemed necessary to enhance nematode efficacy and reduce plant damage in the field.

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