scholarly journals PENGARUH ZAT PENGATUR TUMBUH TERHADAP PEMBENTUKAN DAN PERTUMBUHAN SERTA KANDUNGAN SINENSETIN DALAM KALUS PADA TANAMAN KUMIS KUCING (Orlhosiphon arislatus )

2020 ◽  
Vol 7 (4) ◽  
pp. 99
Author(s):  
SITTI FATIMAH SYAHID ◽  
HERNANI HERNANI
Keyword(s):  
Growth Rate ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Basic Medium ◽  
Second Step ◽  
Ms Medium ◽  
Rate Structure ◽  
Medicinal Crops ◽  
Effect Of Treatment

<p><strong>Effect of growth regulator on the formation, development and sinenselin content of the callus on Orthosiphon aristatus</strong></p><p>Effect of growth regulators on the formation, development and sinenselin Content of the callus on kidney plant ( Orthosiphon arislatus ) was studied al the laboratory of the Research Insitute for Spices and Medicinal Crops ( RISMC ) Bogor from March lo October 2000. Material ( explains ) lor Ihe study was the sterile leaves available in the culture. Basic medium used was Murasige and Skoog ( MS ). The study was conducted in two step. In (he irst step Ihe explains were cultured un MS medium applied with 2 4-1) at the concentration of 0.1; 0.3; 0.5 and 0.7 mg/1. In the second step, the cxplants were cultured on MS medium containing 0 I mg/1 2 4-D (best concentration in Ihe lirsl study) enriched with BA at the concentration of 0.5; 1.0, and 1.5 mg/1. The experiment was designed as a completely randomized in three replicates Eaeh replicate contained live bottles. Parameters used for evaluating Ihe effect of treatment were ihe lime of initiations, growth rate, structure and colour of the calli and sinenselin content which was analyzed with IIPI.C. Results showed thai from the irst experiment, 2.4-1) of 0.1 mg/1 produced Ihe highest rate of calli formation(0.49 g/5 weeks), from Ihe second ex¬periment, il was revealed that Ihe best treatment was 0.5 mg/1 BA Combined with 0.1 mg/1 2 4-1) produced 6.59 g calli/5 weeks, but was not signiicantly different front the calli cultured on the medium containing 1.0 nig/l BA combine with 0.1 mg/1 2.4- D. Result of sinenselin analysis showed thai MS medium containing BA 1 .0 mg/+ 0.1 mg/1 2.4- I) produced (I 24% sinenselin. higher than those of other treatment.</p>

scholarly journals Micropropagation of Little Bluestem (Schizachyrium scoparium L.)

HortScience ◽  
2019 ◽  
Vol 54 (2) ◽  
pp. 348-352 ◽  
Author(s):  
Susan M. Hawkins ◽  
Carol D. Robacker
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Light Level ◽  
The United States ◽  
Callus Cultures ◽  
Schizachyrium Scoparium ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Little Bluestem ◽  
Callus Initiation

Native grasses are increasingly used in the landscape. Little bluestem (Schizachyrium scoparium L.), a perennial bunchgrass native to most of the United States, has ornamental traits, such as variation in leaf color, differences in growth morphology, and attractive seed heads. Traditionally, cultivars of little bluestem are propagated by division, which limits the production of new plants. Our objective in this study was to develop an improved micropropagation protocol for little bluestem that would produce true-to-type plants. In 2016, we cultured immature inflorescences of eight genotypes of little bluestem on Murashige and Skoog (MS) medium with four combinations of kinetin (1.0 or 2.0 mg·L−1) and 2,4-D (0.5 or 1.0 mg·L−1) under three levels of light (dark, semilight, full light) to initiate callus. Cultures were evaluated 30 days after initiation and those that had initiated callus were subcultured. Media for subculturing and rooting contained either 0.1 mg·L−1 or no 1-Naphthaleneacetic acid (NAA). Light level had no effect on callus initiation. Initiation media with 1.0 mg·L−1 kinetin and either level of 2,4-D induced callus at almost twice the rate of media with 2.0 mg·L−1 kinetin, and cultures initiated on those media also produced almost twice the number of rooted plants over all genotypes. Genotype affected the number of rooted plants produced. The addition of NAA to medium for subculturing and rooting did not increase the number of rooted plants. In 2017, we cultured immature inflorescences of four genotypes of little bluestem on MS medium with 0.5 mg·L−1 2,4-D and either 1.0 mg·L−1 kinetin or 6-benzylaminopurine (BAP) under full light. Cultures were evaluated 30 days after initiation. Cultures that had initiated callus were subcultured onto MS medium with the same growth regulators as the initiation medium but without 2,4-D. Cultures were cycled between subculture medium with growth regulator and subculture medium with no additional growth regulator until rooted. Cultures initiated and subcultured on medium with BAP initiated two to three times more callus than those on kinetin and produced twice as many rooted plants. Our recommendation for rapid micropropagation of little bluestem is to initiate cultures on MS medium with 1.0 mg·L−1 BAP and 0.5 mg·L−1 2,4-D. After callus initiation, cultures should be subcultured to medium with BAP but no 2,4-D, alternating with medium with no additional growth regulators, until rooted.

scholarly journals Perkembangan Kultur Daun Aglaonema sp. dengan Perlakuan Kombinasi Zat Pengatur Tumbuh NAA dan 2,4-D dengan BAP (The Leaf Culture Development of Aglaonema sp. Treated by Combination of NAA, 2,4-D and BAP as Growth Regulators)

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Dwi Kusuma Wahyuni ◽  
Dedy Prasetyo ◽  
Sucipto Hariyanto
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Leaf Blade ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Ms Medium ◽  
Leaf Color ◽  
Leaf Culture ◽  
Snow White ◽  
Culture Development

Abstrak Tujuan dari penelitian ini adalah menentukan pengaruh kombinasi NAA dan 2,4-D dengan BAP terhadap perkembangan kultur daun dan konsentrasi yang sesuai untuk induksi kalus tiga kultivar Aglaonema sp. Kultivar-kultivar yang digunakan adalah Dynamic Ruby, Snow White, dan Siam Aurora. Eksplan daun dikulturkan pada medium MS padat dengan perlakuan kombinasi NAA dan 2,4-D dengan BAP. Hasil pengamatan minggu kedelapan menunjukkan ada pengaruh perlakuan zat pengatur tumbuh terhadap perubahan bentuk eksplan daun. Eksplan daun melengkung, bergelombang, membengkak, warna eksplan memucat dan membentuk kalus, tetapi hanya Aglaonema sp. cv. Dynamic Ruby yang mampu membentuk kalus. Kombinasi zat pengatur tumbuh 0,1 ppm 2,4-D and 1 ppm BAP adalah kombinasi yang sesuai untuk induksi kalus untuk Aglaonema sp. cv. Dynamic Ruby. Kata Kunci: Aglaonema, NAA, 2,4-D, BAP, kalus   Abstract   The objective of this study were to determine the effect of combination of NAA and 2,4-D with BAP toward leaf culture development and to know the best concentration of growth regulator substance to induce callus on leaf explant of three Aglaonema cultivars. The cultivars were Dynamic Ruby, Snow White, and Siam Aurora. Leaf explants were cultured on solid MS medium with addition of various concentration combination of NAA and 2,4-D with BAP. Result of eight week observation gave significantly effect to changes shape leaf. Leaf blade was curved or rolled up, swelled, leaf color became pale strands, and formed callus, but the only cultivar which formed callus was Aglaonema sp. cv. Dynamic Ruby. The conclusion of this study was combination of growth regulator substance 0,1 ppm 2,4-D and 1 ppm BAP are the appropriate combination on callus inducing for Aglaonema sp. cv. Dynamic Ruby. Keywords: Aglaonema, NAA, 2,4-D, BAP, callus

scholarly journals Growth regulator effect on in vitro regeneration of rhododendron cultivars

2008 ◽  
Vol 35 (No. 2) ◽  
pp. 90-94 ◽  
Author(s):  
H. Vejsadová
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Indoleacetic Acid ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Commercial Preparation ◽  
High Level

In Rhododendron L. cv. Azuro, Bohumil Kavka, Catharine van Toll, Grandiflorum, Mars, Nova Zembla, Ortrud, Ovation, Prof. Scholz, Purple Splendour, Rebe and Van Werden Poelman, the effect of growth regulators on organogenesis induction of shoot-tip meristems was tested. All cultivars significantly showed the highest shoot regeneration on MS medium containing 6 mg/dm<sup>3</sup> isopentenyladenine (2iP). For most rhododendrons, the highest shoot multiplication was found on a medium with 8&ndash;10 mg/dm<sup>3</sup> 2iP in combination with 1 mg/dm<sup>3</sup> indoleacetic acid (IAA). Shoots rooted successfully in the substrate with high level of peat without growth regulators. However, the commercial preparation Racine significantly increased rooting in cv. Grandiflorum, Nova Zembla and Rebe compared with 0.03% indolebutyric acid (IBA).

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Growth Regulators ◽  
Subsequent Development ◽  
Ms Medium ◽  
Asymbiotic Seed Germination ◽  
Endangered Orchid ◽  
Maximum Root ◽  
Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   

scholarly journals Application of Chondroitin Sulfate on Organogenesis of Two Cymbidium spp. under Different Sources of Lights

2016 ◽  
Vol 8 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Syeda Jabun NAHAR ◽  
Syed M. HAQUE ◽  
Shimasaki KAZUHIKO
Keyword(s):  
Plant Growth ◽  
Chondroitin Sulfate ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Ms Medium ◽  
Food Ingredients ◽  
Blue Led ◽  
Red Led ◽  
Green Led ◽  
Modified Ms Medium

The aim of this study was to present chondroitin sulfate as a plant growth regulator and to give an overview about light effects on PLBs (protocorm like bodies) culture of Cymbidium dayanum and Cymbidium finlaysonianum cultured in vitro. Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars N-acetylgalactosamine and glucuronic acid. It is widely used as a material for food ingredients, cosmetics and medicine. PLBs were cultured on modified MS medium containing different concentration of chondroitin sulfate (0, 0.1, 1 and 10 mg/l), under four sources of lights: conventional white fluorescent tube, red LED, green LED and blue LED. In C. dayanum, 100% PLBs formation rate was observed at 0.1 mg/l chondroitin sulfate with modified MS medium under green LED and 1 mg/l chondroitin sulfate under blue LED; the maximum shoots and roots formation were observed under green LEDs (93% and 80% respectively) when media contained 0.1 mg/l chondroitin sulfate. In C. finlaysonianum, every concentrations of chondroitin sulfate enhanced the growth rate of PLBs when compared to control treatment, under all four sources of lights. The highest values were recorded with 0.1 mg/l chondroitin sulfate which induced 100% PLBs formation under blue LED, while 10 mg/l chondroitin sulfate had induced 100% PLBs formation under green LED. The highest percentage of shoots (73%) was initiated in the medium containing 10 mg/l chondroitin sulfate under green LED. Plant development was strongly influenced by the light quality and plant growth regulator functions as chemical messengers for intercellular communication of plant. The results demonstrated that low concentrations of chondroitin sulfate could promote PLBs, shoots and roots formation of Cymbidium spp. under green and blue LED.

scholarly journals Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

2019 ◽  
Vol 11 (10) ◽  
pp. 277 ◽  
Author(s):  
Meire Pereira de Souza Ferrari ◽  
Mayara dos Santos Queiroz ◽  
Matheus Marquezini de Andrade ◽  
Jessica Rezende Trettel ◽  
Hélida Mara Magalhães
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Activity ◽  
Growth Regulators ◽  
Curcuma Longa ◽  
Ms Medium ◽  
Biochemical Activity ◽  
Root Collar Diameter ◽  
Collar Diameter ◽  
Root Collar

This study aimed to evaluate the in vitro growth and biochemical activity of Curcuma longa explants using different MS medium formulations and growth regulators. In all the experiments, plants were grown in MS medium supplemented with agar (6.5 g L-1) and pH adjusted to 5.8. In the first assay, the MS culture medium at 70% strength, supplemented with 30 g L-1 of sucrose, and without the addition of activated carbon, resulted in the highest number of shoots. The sucrose concentration of 60 g L-1, combined with the addition of actived charcoalin half-strength MS medium, resulted in the increased root dry mass, root collar diameter, and relative chlorophyll index. In the second assay, the highest root collar diameter and dry matter of shoots and roots were found in the MS medium supplemented with 4.44 BAP, 0.46 KIN, and 1.08 NAA. The MS medium with 8.88 BAP, 0.92 KIN, and 2.16 &mu;M NAA resulted in the highest number of shoots (7.75), number of leaves (35), and shoot length (88.57 mm). The antioxidant activity was significantly higher in the treatments that resulted in better plantlets growth performance, demonstrating that the antioxidant activity is related to other factors such as a possible role of growth regulators on the elicitation of compounds in plants. Superoxide dismutase had a high enzymatic activity in both assays, whereas the enzymatic activity of catalase and ascorbate peroxidase was dependent on the culture media used.

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