Effect of different growth regulator combinations on growth rate of explants in walnut in vitro studies using MS medium

Paulownia is a fast-growing woody tree, native to the forests of China. It belongs to the family Scrophulariaceae and is mainly used as a source of wood for furniture and musical instruments. Due to its fast-growing nature and high-quality of wood, there has been growing interest in cultivation and research of Paulownia in Nepal. Growth comparison was performed by measuring shoot length in in vitro condition. Among two species of Paulownia - Paulownia tomentosa (Thunb.) Steud and Paulownia fortuneii (Seem.) Hemsl., the growth rate of P. tomentosa was found to be 0.355 cm/week while that of P. fortuneii was found to be 0.637 cm/week in in-vitro conditions in MS medium supplemented with 0.1 mg/l NAA and 1mg/l BAP. Optimization of rooting methods was also performed, in which, sand rooting was found to be easier and more effective than in-vitro rooting. Dipping the plantlets in 1 mg/l of NAA was found to produce longer and denser roots than lower or higher concentrations during sand rooting.

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Development of an efficient in vitro callus proliferation protocol for edible wild rhubarb (Rheum ribes L.)

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2021.5.11 ◽

2021 ◽

Vol 20 (5) ◽

pp. 119-126

Author(s):

Burcu Tuncer

Keyword(s):

Plant Growth ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Low Dose ◽

Ms Medium ◽

Fresh Weight ◽

Hypocotyl Explants ◽

Callus Proliferation ◽

Callus Regeneration

Rheum ribes L. is a perennial wild species. Young shoots and flower bunches are freshly consumed, and root and rhizomes are generally used for medicinal purposes. The aim of the present study was to improve the callus proliferation protocol for R. ribes L. under in vitro conditions. For callus induction, hypocotyl explants taken from 14-day old plantlets germinated in Murashige and Skoog (MS) media were cultured in MS media with 9 plant growth regulator (PGR) combinations containing 6-benzylaminopurine (BAP) (2, 3, and 4 mg/L) + naphthylacetic acid (NAA) (0.1, 0.5, and 1 mg/L). Then, for callus proliferation, 4 PGR combinations containing NAA (0.2 mg/L) + thidiazuron (TDZ) (0.5, 1, 2, and 3 mg) were used in the first set of experiments, and 36 PGR combinations containing BAP (1, 2, 3, and 4 mg/L) + indole-3-butyric acid (IBA) (0.2, 0.5, and 1 mg/L), BAP (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L), and TDZ (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L) were used in the second set of experiments. At the end of the second set of experiments, the greatest callus regeneration ratios were obtained due to the combinations including BAP and IBA as well as the low-dose TDZ- (especially 1 mg/L) and NAA- (0.2, 0.5, 1 mg/L) combinations. Regarding callus fresh weights, TDZ + NAA combinations were found to be more successful. The greatest callus fresh weight (12.7 ±0.4 g) was obtained from MS medium supplemented with 2 mg/L TDZ and 0.2 mg/L NAA.

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PENGARUH ZAT PENGATUR TUMBUH TERHADAP PEMBENTUKAN DAN PERTUMBUHAN SERTA KANDUNGAN SINENSETIN DALAM KALUS PADA TANAMAN KUMIS KUCING (Orlhosiphon arislatus )

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v7n4.2001.99-103 ◽

2020 ◽

Vol 7 (4) ◽

pp. 99

Author(s):

SITTI FATIMAH SYAHID ◽

HERNANI HERNANI

Keyword(s):

Growth Rate ◽

Growth Regulators ◽

Growth Regulator ◽

Basic Medium ◽

Second Step ◽

Ms Medium ◽

Rate Structure ◽

Medicinal Crops ◽

Effect Of Treatment

Effect of growth regulator on the formation, development and sinenselin content of the callus on Orthosiphon aristatusEffect of growth regulators on the formation, development and sinenselin Content of the callus on kidney plant ( Orthosiphon arislatus ) was studied al the laboratory of the Research Insitute for Spices and Medicinal Crops ( RISMC ) Bogor from March lo October 2000. Material ( explains ) lor Ihe study was the sterile leaves available in the culture. Basic medium used was Murasige and Skoog ( MS ). The study was conducted in two step. In (he irst step Ihe explains were cultured un MS medium applied with 2 4-1) at the concentration of 0.1; 0.3; 0.5 and 0.7 mg/1. In the second step, the cxplants were cultured on MS medium containing 0 I mg/1 2 4-D (best concentration in Ihe lirsl study) enriched with BA at the concentration of 0.5; 1.0, and 1.5 mg/1. The experiment was designed as a completely randomized in three replicates Eaeh replicate contained live bottles. Parameters used for evaluating Ihe effect of treatment were ihe lime of initiations, growth rate, structure and colour of the calli and sinenselin content which was analyzed with IIPI.C. Results showed thai from the irst experiment, 2.4-1) of 0.1 mg/1 produced Ihe highest rate of calli formation(0.49 g/5 weeks), from Ihe second ex¬periment, il was revealed that Ihe best treatment was 0.5 mg/1 BA Combined with 0.1 mg/1 2 4-1) produced 6.59 g calli/5 weeks, but was not signiicantly different front the calli cultured on the medium containing 1.0 nig/l BA combine with 0.1 mg/1 2.4- D. Result of sinenselin analysis showed thai MS medium containing BA 1 .0 mg/+ 0.1 mg/1 2.4- I) produced (I 24% sinenselin. higher than those of other treatment.

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A Novel Method for Rapid Micropropagation of Pineapple

HortScience ◽

10.21273/hortsci.30.1.127 ◽

1995 ◽

Vol 30 (1) ◽

pp. 127-129 ◽

Cited By ~ 23

Author(s):

E. Kiss ◽

J. Kiss ◽

G. Gyulai ◽

L.E. Heszky

Keyword(s):

Growth Regulator ◽

Shoot Elongation ◽

Ananas Comosus ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Regeneration Rate ◽

Novel Method ◽

N6 Medium ◽

Regenerated Plantlets

A novel micropropagation method for pineapple (Ananas comosus L.), based on shoot elongation induced in vitro, was demonstrated for two cultivars. Decapitated in vitro plantlets were used as explants. Shoot etiolation was induced by placing explants in a Murashige and Skoog (MS) medium containing NAA (10 μm) and incubating in darkness at 28C for 30 to 40 days. The mean number of the regenerated etiolated shoots per explant was 2.6 ± 0.29. The etiolated shoots were placed into N6 medium supplemented with kinetin or BA (25 or 20 μm, respectively). After 4 to 6 weeks, shoots regenerated along the nodes. The highest regeneration rate was 15 and 13 plantlets per node with 25 μm kinetin and 20 mm BA, respectively. Regenerated plantlets were rooted on a growth-regulator-free MS medium. Residual shoots of the initial explants could be recycled by rooting on a growth-regulator-free MS medium. This procedure enables the regeneration of several thousand plantlets per year. Chemical names used: naphthaleneacetic acid (NAA); benzyladenine (BA).

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Differential effects of synthetic media on long-term growth, starch accumulation and transcription of ADP-glucosepyrophosphorylase subunit genes in Landoltia punctata

Scientific Reports ◽

10.1038/s41598-019-51677-w ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Chokchai Kittiwongwattana

Keyword(s):

Growth Rate ◽

Starch Content ◽

Morphological Characteristics ◽

Correlation Coefficients ◽

Starch Biosynthesis ◽

Starch Accumulation ◽

Ms Medium ◽

Expression Of Genes ◽

Synthetic Media

Abstract Murashige & Skoog (MS) and Hoagland’s media were previously used for in vitro culture of Landoltia punctata. During subsequent ex vitro culture, the use of MS medium resulted in a higher growth rate, compared to Hoagland’s medium. Thus, a higher starch content of L. punctata in MS medium was previously hypothesized. Here, L. punctata strain 5632 was isolated and characterized using morphological characteristics and the atpF-atpH intergenic region. During early cultivation stage, fresh weight and relative growth rate in MS medium were lower than Hoagland’s medium. Conversely, starch content in MS medium was considerably higher than in Hoagland’s medium. Medium effects on expression of genes coding for starch-biosynthesis ADP-glucosepyrophosphorylase (AGPase) were determined. Genomic fragments of small (LeAPS) and large (LeAPL1) AGPase subunits were characterized. Differential expression between each AGPase subunit genes was observed in both media. Additionally, in MS medium, the highest correlation coefficients between starch content and gene expression was found with LeAPS (0.81) and followed by LeAPL3 (0.67), LeAPL2 (0.65) and LeAPL1 (0.28). In Hoagland’s medium, the coefficients of LeAPL3 (0.83) and LeAPL2 (0.62) were higher than LeAPS (0.18) and LeAPL1 (−0.62). This suggested different levels of contributions of these genes in starch biosynthesis in both media.

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PENGARUH ARANG AKTIF, BENZILADENIN, DAN KINETIN TERHADAP PERTUMBUHAN TUNAS JATI SOLOMON (Tectona grandis Linn. f) IN VITRO

Jurnal Ilmiah Biologi dan Keanekaragaman Hayati ◽

10.23960/jbekh.v5i2.48 ◽

2019 ◽

Vol 5 (2) ◽

pp. 21-30

Author(s):

Husen Hariadi ◽

Yusnita Yusnita ◽

Melya Riniarti ◽

Dwi Hapsoro

Keyword(s):

Growth Regulator ◽

Activated Charcoal ◽

Experimental Treatment ◽

Tectona Grandis ◽

Ms Medium ◽

Culture Techniques ◽

Shoot Bud ◽

Randomized Design ◽

Number Of Leaves

Tissue culture techniques can be used for propagation of uniformaly large teak solomon seeds.The purpose of this research was knowing the effect of activated charcoal, the addition of benzyladenine (BA) and combination of BA with 6-furfurylaminopurine (kinetin) to the growth of shoots of solomon teak in vitro. The solomon teak explants used were singlestem cuttings from aseptic shoots obtained from in vitro cultures. This research was conducted in laboratory with complete randomized design with 3 replications. The experimental treatment was a single factor consisting of basic MS medium (Murashige and Skoog, 1962), with 6 treatments: MS without growth regulator (control), MS without growth regulator + 2 g/l activated charcoal, MS + 0,1 m/l BA , MS + 0,2 m/l BA, MS + 0,1 m/l BA + 0,1 m/l kinetin and MS + 0,2 m/l BA + 0,1 m/l kinetin. Observation on the number of books/ shoots, number of leaves/ shoots, shoot/ bud height and visual apperance of culture was taken at 8 weeks after planting. The data were analyzed for variety and continue the separation of the LSD at 5% level. The results showed that in general, all six treatments could be used for propagation of in vitro teak solomon (Tectona grandis Linn. f) and produced at least 6,22 books/ shoots every 8 weeks. The best media were MS medium + 0,1 m/l BA and MS + 0,1 m/1 BA + 0,1 m/l kinetin, because it able to produce 7,78 books/ shoots. The highest number of leaves was obtained at the treatment of MS + 0,1 m/l BA, while the average shoots/ shoots produced were not different for all.

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Pembentukan mother plant Bacopa australis secara In-vitro dan aklimatisasi dalam aquascape air tawar

Media Akuakultur ◽

10.15578/ma.12.2.2017.85-94 ◽

2017 ◽

Vol 12 (2) ◽

pp. 85

Author(s):

Media Fitri Isma Nugraha ◽

Rossa Yunita ◽

Endang Gati Lestari ◽

Idil Ardi

Keyword(s):

Growth Regulator ◽

In Vitro Propagation ◽

Aquatic Plant ◽

Agricultural Land ◽

Freshwater Ecosystems ◽

Mother Plant ◽

Silica Sand ◽

Ms Medium ◽

Liquid Fertilizer

Tanaman air adalah bagian penting dari ekosistem air tawar. Salah satu spesies yang terkenal adalah Bacopa australis. Hobiis aquascape saat ini memiliki ketertarikan tinggi terhadap tanaman air dengan kualitas yang bagus dari setiap spesiesnya. Metode perbanyakan tanaman air tanpa tanah, lahan pertanian dan air perlu dilakukan untuk memenuhi keinginan tersebut. Tujuan dari penelitian ini adalah untuk mendapatkan formula media kultur jaringan dan zat pengatur tumbuh yang tepat untuk multiplikasi dalam perakitan mother plant (tanaman induk) Bacopa australis, serta mendapatkan media terbaik untuk aklimatisasi. Media yang digunakan adalah media Murashige dan Skoog (MS) A padat dengan perbedaan konsentrasi zat pengatur tumbuh. Perlakuan uji dalam kombinasi zat pengatur tumbuh (a) 0,50 mg/L BAP + 0,50 mg/L kinetin; (b) 0,50 mg/L BAP; dan (c) 0,50 mg/L 2,4-D. Aklimatisasi tanaman induk dilakukan pada berbagai media antara lain 1) pasir silika + pupuk aqua soil amazonia, 2). pasir malang + pupuk aqua soil amazonia, 3) pasir silika + pupuk cair; 4) pasir malang + pupuk. Hasil yang diperoleh, yaitu formula media kultur terbaik untuk multiplikasi tunas tanaman B. australis secara in-vitro adalah media MS (A) yang diperkaya dengan 0,5 mg/L BAP + 0,5 mg/L kinetin, sedangkan aklimatisasi terbaik pada media pasir malang + pupuk aqua soil amazonia.Water plant is an important part of freshwater ecosystems. One of the famous species is Bacopa australis. Today, many aquascape hobbyists have a high interest in aquatic plant species that have good aesthetic appearances. To answer this challenge, a new method in-vitro propagation of aquatic plants, planted without soil, agricultural land and water was conducted. The aim of this research was to find the best growth regulator hormon formula and aclimatisation medium, in creating the mother plant Bacopa australis. The medium used was MS (Murashige and Skoog, 1974) with different growth regulator hormon, i.e: (a) 0.50 mg L-1 BAP + 0.50 mg L-1 kinetin, (b) 0.50 mg L-1 BAP, (c) 0.50 mg L-1 2.4-D. The aclimatisation of the mother plant candidates used four treatments, i.e: (1) silica sand + aqua soil amazonia fertilizer, (2) malang sand + aqua soil amazonia fertilizer, (3) silica sand + liquid fertilizer, (4) malang sand + liquid fertilizer. The results showed that the best formula for in-vitro multiplication mother plant of Bacopa australis was MS medium with 0.5 mg/L BAP + 0.5 mg/L kinetin (treatment A). The best medium aclimatisation was malang sand + aqua soil amazonia fertilizer medium.

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Analysis the effect of plant growth regulator (Gibgro-20T) on seaweed Eucheuma cottonii by in vitro

Akuatikisle Jurnal Akuakultur Pesisir dan Pulau-Pulau Kecil ◽

10.29239/j.akuatikisle.5.1.15-19 ◽

2021 ◽

Vol 5 (1) ◽

pp. 15-19

Author(s):

Muhammad Kasnir ◽

Khairun Nisaa ◽

Harlina Harlina ◽

Masyita Masyita

Keyword(s):

Growth Rate ◽

Plant Growth ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Length Growth ◽

Highest Weight ◽

Weight Growth ◽

Randomized Design ◽

Eucheuma Cottonii

This study aims to determine the effect of the dose of plant growth regulator (PGR) Gibgro-20T on the growth of Eucheuma cottonii in-vitro. The experimental method used was a Completely Randomized Design (CRD) treatment with a Gibgro-20T dose of 0 ppm (control), 5 ppm, 10 ppm, and 15 ppm. The results showed that the highest weight growth rate (RGRW) obtained at 10 ppm PGR dose, which is 1.27% per day. Meanwhile, the highest length growth rate (RGRL) obtained at a dose of 10 ppm, which was 1.72% per day, and the highest RGRL was significantly different from the control (0 ppm) and 5 ppm but was not different real (P<0.05) with 15 ppm treatment. Using Gibgro-20T at doses of 5, 10, and 15 ppm, however, doses of 10 and 15 ppm show higher length growth.

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KEMAMPUAN TUMBUH EKSPLAN Jatropha curcas L. PADA MEDIA IN VITRO YANG MENGANDUNG HORMON IBA DAN BA

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v1i1.545 ◽

2014 ◽

Vol 1 (1) ◽

pp. 1

Author(s):

Karyanti . ◽

Juanda . ◽

Teuku Tajuddin

Keyword(s):

Growth Rate ◽

Jatropha Curcas ◽

Callus Formation ◽

Ms Medium ◽

Liquid Media ◽

Jatropha Curcas L ◽

Solid Media ◽

Number Of Leaves ◽

Growth Ability

GROWTH ABILITY OF Jatropha curcas L. EXPLANTS ON THE IN VITRO MEDIA CONTAINING IBA AND BAResearch on the growth ability of Jatropha curcas L. shoots and callus in solid and liquid media have been conducted. Explants were planted in the initiation MS medium. After ten weeks, the explants were subcultured into solid and liquid media containing combination of IBA and BA treatments. The number of combinations was 12 treatments, each with 6 replications. Observation was conducted from the first week after subculturing upto the fourth week. Parameters of observation were the percentage of explant forming shoots, the number of shoots, height, number of leaves, weight, color, and form of callus. The results showed that the explant which was subcultured in liquid media had higher growth rate than those subcultured in solid media. Treatment of 1 ppm IBA + 0.5 ppm BA gave a good result on the growth of shoots on solid and liquid media. For callus formation, treatment of 2 ppm IBA + 1 ppm BA gave the best result.Keywords: Callus, Jatropha, IBA and BA, solid and liquid media, hormone ABSTRAKPenelitian terhadap kemampuan tumbuh kalus dan tunas tanaman jarak pagar (Jatropha curcas L.) telah dilakukan pada media padat dan cair. Eksplan diinisiasi pada media MS dan setelah 10 minggu dipindahkan ke media padat dan cair yang mengandung perlakuan kombinasi hormon IBA dan BA. Jumlah kombinasi sebanyak 12 perlakuan dan setiap perlakuan dibuat 6 ulangan. Pengamatan dilakukan dari minggu pertama subkultur hingga minggu keempat. Peubah yang diamati adalah persentase eksplan yang membentuk tunas, jumlah dan tinggi tunas, terbentuknya daun pada tunas, perbedaan berat, bentuk, dan warna kalus. Hasil yang diperoleh menunjukkan bahwa eksplan yang disubkultur pada media cair memiliki laju pertumbuhan yang lebih tinggi daripada media padat. Perlakuan IBA 1 ppm + BA 0,5 ppm menghasilkan pertumbuhan tunas yang paling tinggi pada media padat dan cair. Pembentukan kalus yang terbaik diperoleh pada perlakuan IBA 2 ppm + BA 1 ppm.Kata kunci: Kalus, Jatropha, IBA dan BA, media padat dan cair, hormon

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Growth regulator effect on in vitro regeneration of rhododendron cultivars

Horticultural Science ◽

10.17221/643-hortsci ◽

2008 ◽

Vol 35 (No. 2) ◽

pp. 90-94 ◽

Cited By ~ 3

Author(s):

H. Vejsadová

Keyword(s):

Growth Regulators ◽

Growth Regulator ◽

Indoleacetic Acid ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Ms Medium ◽

Indolebutyric Acid ◽

Commercial Preparation ◽

High Level

In Rhododendron L. cv. Azuro, Bohumil Kavka, Catharine van Toll, Grandiflorum, Mars, Nova Zembla, Ortrud, Ovation, Prof. Scholz, Purple Splendour, Rebe and Van Werden Poelman, the effect of growth regulators on organogenesis induction of shoot-tip meristems was tested. All cultivars significantly showed the highest shoot regeneration on MS medium containing 6 mg/dm3 isopentenyladenine (2iP). For most rhododendrons, the highest shoot multiplication was found on a medium with 8–10 mg/dm3 2iP in combination with 1 mg/dm3 indoleacetic acid (IAA). Shoots rooted successfully in the substrate with high level of peat without growth regulators. However, the commercial preparation Racine significantly increased rooting in cv. Grandiflorum, Nova Zembla and Rebe compared with 0.03% indolebutyric acid (IBA).

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