scholarly journals Effect of aqueous and alcohol (phenol) extract from cyperus rotundus on mitotic Division in tap roots of Allium cepa

2013 ◽  
Vol 10 (4) ◽  
pp. 1112-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Allium Cepa ◽  
Aqueous Extract ◽  
Mitotic Division ◽  
Cyperus Rotundus ◽  
Control Treatment ◽  
Alcoholic Extract ◽  
Phenol Compound ◽  
Layer Chromatography ◽  
Crude Aqueous Extract ◽  
Phenol Extract

This study was conducted to test the effect of aqueous and alcoholic extracts for cyperus rotundus on the mitosis in tap roots of Allium cepa. the result of general an identical qualitative tests showed contains certain compounds that of crude aqueous and alcoholic extract, Used as five different concentrations of (10, 20.38, 56, 75) mg / ml for a period of four hours of treatment. After the chemical has been detected for some preliminary chemical compounds of the crude aqueous extract, while the alcoholic extract either phenol compound has been detected for phenols using several techniques included the use of thin layer chromatography TLC and measurement of disability factor RF and the degree of fusion and measurement of absorbance. The results showed that the crude aqueous extract and alcoholic extract and phenol their impact inhibitive as decreased normal division in all concentrations, where it decreased to 50% of the control treatment at the highest concentration also led all transcription to stop cells in metaphase at different rates and there were some chromosomal aberration including Bridges.

scholarly journals Effect of Supplementing Alcoholic and Aqueous Extract of Khalal and Seedless Date and Date Seed Khalal AL-Zahdi Date (Phoenix Dactylifera L.) to Drinking Water on Some Physiological and Microbial Traits of Broiler Reared Under High Temperature

2021 ◽  
Vol 910 (1) ◽  
pp. 012019
Author(s):  
Mohammed Rasool Mahdi Jasim ◽  
Dhia Kalil Ibrahim
Keyword(s):  
Drinking Water ◽  
High Temperature ◽  
Blood Serum ◽  
Aqueous Extract ◽  
Phoenix Dactylifera ◽  
Oxidative Enzymes ◽  
Control Treatment ◽  
Alcoholic Extract ◽  
Date Seed ◽  
Phoenix Dactylifera L

Abstract This research aims to effect of supplementing alcoholic and aqueous extract of khalal and seedless date and date seed khalal AL-Zahdi date (Phoenix dactylifera L.) to drinking water on some of the physiological, histological and microbial traits of broilers reared under high temperature, As the study included the use of 300 chicks of one day age and with an average weight of 41 g, the unsexed rose strain, and it was distributed to five treatments, where one treatment contained 60 chicks, with three replicates for each treatment (20 chicks/replicates ) for a period of 6 weeks, the birds were exposed to temperatures at an average of (28-36 -30 ± 2 ° C) and the degree of humidity at an average of (40-60-50 ± 2%) for the times (700-1200-1900). The chicks were distributed randomly into five treatments as follows: - The first treatment (T1), the control treatment, without any addition to drinking water, the second treatment (T2) and the third (T3), adding 300 mg/liter of water from the aqueous and alcoholic extract for Al-Zahdi khalal, respectively and the fourth treatment (T4) and the fifth (T5) adding 300 mg/liter of water from aqueous and alcoholic extract for the Al-Zahdi khalal seed, respectively. The results of the study indicated that there was a significant improvement in the weekly body temperature, a significant increase in the number of lymphocytes and a significant decrease in the number of heterophill cells and the percentage of heterophill cells to lymphocytes (H/L) for all addition factors compared to With control treatment, As well as a significant increase in the concentration of total protein in blood serum and all oxidative enzymes in blood serum, noting that a significant decrease occurred in the concentration of both glucose and cholesterol at the age of 21 days and the AST enzyme and ALT enzyme at the age of 42 days. The preference was in favor of T3 and T5, as was the significant increase in all the studied histological traits. Also, there was a significant decrease in the number of E. Coli bacteria in the ileum area only, and a significant increase in the number of Lactobacilli bacteria in the jejunum and ileum regions. We conclude from the study that the use of these extracts contributed to the improvement of the studied physiological, histological and microbial characteristics of broilers reared at high temperatures, noting that T3 and T5 recorded the best results for all the studied traits.

scholarly journals Cytogenetic effect of crude aqueous extract from leaf of Lycium barbarum on root tip of Allium cepa L.

2008 ◽  
Vol 5 (4) ◽  
pp. 492-499
Author(s):  
Baghdad Science Journal
Keyword(s):  
Mitotic Index ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Sublethal Effect ◽  
Root Tip ◽  
Lycium Barbarum ◽  
Phase Index ◽  
Allium Cepa L ◽  
Crude Aqueous Extract

This study was carried out to investigate the cytogenetic effects of crude aqueous extract of Lycium barbarum on the roots tip of Allium cepa Using three concentration 125, 25, 50 mg/ ml for 2, 4, 6 hours treatment periods.This study were included some cytogenetic analysis such as mitotic index , phase index and chromosome aberration. The data showed that the treatment with 50mg/ml for 6huors led to reduce the mitotic index less than 50% . This reduction considered to have toxic and sublethal effect . These results revealed mutagenic potency by inducing differents type of chromosome aberration.

Therapeutic efficacy of some extracts of hot pepper fruits (Capsicum annuum) and Sumac seeds (Rhus coriaria) on the growth of some isolated dermatophytes species

2018 ◽  
Vol 1 (3) ◽  
pp. 52-62
Author(s):  
Sara Omran ◽  
Abdulghani Alsamarai ◽  
Firas Razzzaq
Keyword(s):  
Antifungal Activity ◽  
Fungal Infection ◽  
Aqueous Extract ◽  
Plant Extracts ◽  
Therapeutic Efficacy ◽  
Diffusion Method ◽  
Quantitative Detection ◽  
Hot Pepper ◽  
Alcoholic Extract ◽  
Dermatology Clinic

Background: Fungal infections are one of the common skin diseases with difficulty in their treatment approach. The present efficient drugs for fungal infection are limited. Aim: To determine the therapeutic efficacy of plant extracts as alternative antifungal agents. Materials and methods: 100 clinical samples [68 from female and 32 from male] were collected during the period from March to July 2017 from subjects attending Dermatology Clinic in Salah Uldean General Hospital. Fungal infection was diagnosed with using KOH wet preparation. Fungal species identified by using conventional approach. The active ingredients existing in the plant extracts were detected and analyzed through qualitative and quantitative detection technique of chemical compounds using a high performance liquid chromatographic device (HPLC). Agar diffusion method was used to determine antifungal activity of plant extracts. Results: Direct microscopic examination showed that there were (75%) positive samples, while culture shows (67%) positive samples. The isolated dermatophytes belong to Epidermophyoton, Microsporum, and Trichophyton genus. The predominant dermatophytes were T. rubrum (25%) species. The highest frequency of infection was in the age group of 11-20 years. The sensitivity of the tested fungi to the aqueous and alcoholic plant extracts varies. Alcoholic extract of the hot pepper plant was more effective as antifungal than the aqueous extract of the same plant. However, aqueous hot pepper extracts was more effective against T. mentagrophyte than that of alcoholic extract. Additionally, alcoholic Sumac extract shows higher efficacy that aqueous extract. Conclusion: Hot pepper and Sumac extracts show antifungal activity against Microsporum canis, Trichophyton rubrum and T. mentagrophyte.

scholarly journals Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3303
Author(s):  
Amina M. G. Zedan ◽  
Mohamed I. Sakran ◽  
Omar Bahattab ◽  
Yousef M. Hawsawi ◽  
Osama Al-Amer ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Individual Treatment ◽  
Alcoholic Extract ◽  
Anticancer Effect ◽  
Mcf7 Cells ◽  
Vespa Orientalis ◽  
Anti Inflammatory

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.

scholarly journals ALLELOPATHIC POTENTIAL OF Cyperus rotundus L. EXTRACTS ON GERMINATION AND COWPEA SEEDLING ESTABLISHMENT

Nativa ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 261
Author(s):  
João Alves Ferreira Pereira ◽  
Tatiana Maria da Silva ◽  
Andreza Raquel Barbosa de Farias ◽  
Alexandre Bosco de Oliveira
Keyword(s):  
Seed Germination ◽  
Vigna Unguiculata ◽  
Early Growth ◽  
Shoot Length ◽  
Cyperus Rotundus ◽  
Control Treatment ◽  
Root Weight ◽  
Allelopathic Potential ◽  
Germination Speed ◽  
Allelopathic Effects

POTENCIAL ALELOPÁTICO DE EXTRATOS DE Cyperus rotundus L. NA GERMINAÇÃO E ESTABELECIMENTO DE PLÂNTULAS DE FEIJÃO-CAUPI Este trabalho investigou os efeitos alelopáticos de extratos de Cyperus rotundus L. na germinação de sementes e crescimento inicial das plântulas de Vigna unguiculata L. Walp. Extratos alcoólicos nas concentrações 0; 1,5%; 3,0%; 4,5%; 6,0%; 7,5% e 9,0%, obtidos a partir da trituração de folhas de C. rotundus L. foram utilizados para umedecimento das folhas de papel toalha “germitest”. Os dados foram submetidos a análise de variância pelo F e as médias comparadas pelos testes de Tukey e Kruscal-Wallis, ambos a 5% de probabilidade. A primeira contagem de germinação, índice de velocidade de germinação, comprimento de raiz, comprimento da parte aérea e massa seca da raiz do feijão-caupi foram analisados de forma paramétrica, sendo constatado que apenas houve significância na variável primeira contagem e uma redução na germinação em relação ao tratamento controle em torno de 10%. Nas condições em que essa pesquisa foi realizada, os extratos alcoólicos de C. rotundus L. não afetam a germinação de sementes e crescimento inicial das plântulas de feijão-caupi.Palavras-chave: tiririca, alelopatia, interferência, plantas daninhas. ABSTRACT:This study aimed to investigate the allelopathic effects of Cyperus rotundus L. extracts on seed germination and early development of seedlings of Vigna unguiculata L. Walp. Alcoholic extracts at concentrations of 0, 1.5%, 3%, 4.5%, 6.0%, 7.5%, and 9.0% were obtained by crushing C. rotundus L. leaves used to moisten the “germitest” paper sheets. The data were subjected to analysis of variance and also both Tukey and Kruscal-Wallis tests were carried out at 5% for the data means. The first germination count, germination speed index, root length, shoot length, and dry root weight of cowpea were parametrically analyzed. The only variable with significance was first germination count. The germination reducted in relation to the control treatment around 10%. Thus, in the conditions used in this research, alcoholic extracts of C. rotundus L. did not affect either seed germination or early growth of cowpea seedlings.Keywords: nut grass, allelopathy, interference, weeds. DOI:

scholarly journals Inhibitory Potential of Murraya Koenigii (L.) and Ficus Carica L. Extracts Against Aldose Reductase (ALR), Advanced Glycation End Products (AGEs) Formation and Sorbitol Accumulation

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shah Asma Farooq ◽  
Randhir Singh
Keyword(s):  
Aqueous Extract ◽  
Microvascular Complications ◽  
Wistar Rat ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Ficus Carica ◽  
Alcoholic Extract ◽  
Murraya Koenigii ◽  
Treatment Of Diabetes ◽  
Inhibitory Potential

Abstract Introduction: Murraya koenigii (L.) and Ficus carica L. are traditionally used plants with significant medicinal and nutritional values. Aim and Objective: The present study was focused on the evaluation of hydro-alcoholic and aqueous extracts of M. koenigii (L.) leaves [MKHA (M. koenigii (L.) hydro-alcoholic extract) and MKAQ (M.koenigii (L.) aqueous extract)] and dried fruits of F. carica L. [FCHA (F. carica L. hydro-alcoholic extract) and FCAQ (F. carica L. aqueous extract)] in the attenuation of markers of microvascular complications associated with diabetes mellitus which can be further used to investigate the pharmacological activity of these plants in treatment of diabetes and its complications. Material and Method: The attenuating effect of the extracts was evaluated by calculating the ALR1 enzyme inhibition in a kidney of Wistar rat, anti-glycation activity in bovine serum albumin (BSA) and erythrocyte sorbitol accumulation inhibition in heparinized human blood. Results: A significant inhibitory effect (IC50 6.47μg/ml,7.26μg/ml,8.93 μg/ml and 9.66μg/ml) was observed with different concentrations of extracts (MKHA, MKAQ, FCHA and FCAQ) respectively, against ALR enzyme. After the 4th week of incubation, the inhibition of AGEs formation by MKHA, MKAQ, FCHA and FCAQ (500μg/ml) was found to be 82.58%, 78.58%, 74.39% and 69.56% respectively. MKHA, MKAQ, FCHA and FCAQ were found to exhibit significant inhibition against the accumulation of sorbitol in RBCs with IC50 188.88 μg/ml, 247.74μg/ml, 291.94μg/ml and 345.34μg/ml, respectively. Conclusion: The administration of different concentrations of MKHA, MKAQ, FCHA and FCAQ significantly attenuated ALR, AGEs and sorbitol accumulation; hence, it can provide a basis for identification and development of new inhibitors of these biomarkers.

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