scholarly journals Impact of Culture Media Composition, Nutrients Stress and Gamma Radiation on Biomass and Lipid of the Green Microalga, Dictyochloropsis splendida as a Potential Feedstock for Biodiesel Production

2021 ◽  
Vol 19 (1) ◽  
Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 129-136
Author(s):  
Velichka Rodeva ◽  
Stanislava Grozeva ◽  
Velichka Todorova

Callusogenesis and regeneration ability of cotyledon and hypocotyl explants from three Bulgarian pepper varieties in MS basal medium supplemented with l-3mg/l BAP. l.0mg/1 IAA and 0.5mg/l GA3 was studied. In the different variants of culture medium was registered high level of callusogenesis and organogenesis in both type of explants from the all varieties. The highest percentage of plant-regenerants is established in cotyledon explants (from 3.3 to 18.3) in variant 3 of the culture medium containing 3mg/l BA. In the process of micropropagation by stem explants of the same studied pepper varieties the addition of the vitamins C. B12. Casein hydrolysate and Ferulic acid had a stimulation effect on the plant growth in height and rooting. In result of anther cultivation from three pepper varieties and four breeding lines the highest percentage of embryo structure formation was registered in varieties Albena and Strjama (12.0 and 13.8 respectively). The Bulgarian peppers are recalcitrant and their in vitro answer is different depending from the explants type, genotype and the culture media composition.


2011 ◽  
Vol 63 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Virginia A. Herrera-Valencia ◽  
Patricia Y. Contreras-Pool ◽  
Silvia J. López-Adrián ◽  
Santy Peraza-Echeverría ◽  
Luis F. Barahona-Pérez

2004 ◽  
Vol 16 (2) ◽  
pp. 15 ◽  
Author(s):  
Matthew B. Wheeler ◽  
Sherrie G. Clark ◽  
David J. Beebe

Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.


e-xacta ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 11
Author(s):  
Najla Postaue ◽  
Leila Cristina Moraes ◽  
Rosa Maria Farias Asmus

A biomassa de microalgas tem apresentado potencial para produção de biodiesel, contudo a viabilidade do cultivo de microalgas depende de fonte de nutrientes de baixo custo. O presente estudo objetivou utilizar o chorume como fonte de nutrientes para microalgas. Os experimentos foram conduzidos visando avaliar a obtenção da biomassa microalgal, conversão de lipídios e rendimento em ésteres metílicos de ácidos graxos, para os meios de cultivos utilizando 5%, 12% e 20% de chorume, com concentrações de 0,02, 0,05 e 0,08 g N. L-1 e para meio de controle contendo 1% de, Nitrogênio (N), Fósforo (P) e Potássio (K), na concentração de 20 g L-1, 5 g L-1 e 20 g L-1, respectivamente. A microalga utilizada neste trabalho foi a de classe Chlorophyceae e família Coccomyxaceae. Os resultados demonstraram que o meio com concentração de 12% de chorume obteve melhores resultados, possibilitando alcançar 1,19 g de biomassa, conversão de 108,15 mg g-1 de lipídios e conteúdo de ésteres de 410,77mg g-1, a microalga utilizada apresentou ainda predominância dos ácidos graxos palmítico e oleico, apresentando baixa quantidade de ácidos graxos saturados o que pode fornecer ao combustível, resistência ao frio. E tais aspectos demonstraram que o chorume pode ser uma fonte promissora de nutrientes para o cultivo das microalgas estudadas. AbstractMicroalgae biomass has presented potential for biodiesel production, however the viability of microalgae cultivation depends on low cost nutrient source. The present study aimed to use leachate as a source of nutrients for microalgae. The experiments were conducted to evaluate the microalgal biomass, lipid conversion and yield in fatty acid methyl esters, for the culture media using 5%, 12% and 20% leachate, with concentrations of 0.02, 0.05 and 0.08 g N. L-1 and for control medium containing 1% Nitrogen (N), Phosphorus (P) and Potassium (K), at a concentration of 20 g L-1, 5 g L-1 and 20 g L-1, respectively. The microalgae used in this work was Chlorophyceae class and Coccomyxaceae family. The results showed that the medium with a concentration of 12% of leachate obtained better results, allowing to reach 1.19 g of biomass, conversion of 108.15 mg g-1 of lipids and esters content of 410,77 mg g-1. The microalgae used also presented predominance of palmitic and oleic fatty acids, presenting low amount of saturated fatty acids which can provide the fuel with cold resistance. And these aspects demonstrated that the leachate can be a promising source of nutrients for the cultivation of the studied microalgae.


2010 ◽  
Vol 25 (1) ◽  
pp. 101
Author(s):  
Sri Hartati

<p>This research aimed to study the effect of kinds of culture media composition added with organic matter (soya bean extract, corn extract and fish emulsion) and PGR concentration to the growth of each cross breed orchid explants. Research was conducted in Plant Physiology and Biotechnology Laboratory, Agriculture Faculty, Sebelas Maret University of Surakarta from March 2009 until the end. This research was done in Randomized Completely Design with two factors of treatment and four replications. The first factor was media and organic matter, consist of 3 levels: soya bean extract, corn extract and fish emulsion. The second factor was atonik concentration, consist of 3 levels: 0 cc/l, 0.5 cc/l and 1 cc/l. The research concluded that for the explants of crossing (Phalaeonopsis pinlong cinderela ♀ &gt;&lt; Phalaeonopsis joanekileup June” ♂) two treatment of corn extract affected significantly to the root emergence time (21.33 ) and member of root (1,92 day after planting) The treatment of fish emulsion affected significantly to the root length (2,82 cm), number of root (1,92) and produced the longest leaf. The treatment of Atonik increased the number of leaf and root.</p>


Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1177
Author(s):  
Fereshteh Moradi ◽  
Christopher Moffatt ◽  
Jeffrey A. Stuart

Cancer cell culture is routinely performed under superphysiologic O2 levels and in media such as Dulbecco’s Modified Eagle Medium (DMEM) with nutrient composition dissimilar to mammalian extracellular fluid. Recently developed cell culture media (e.g., Plasmax, Human Plasma-Like Medium (HPLM)), which are modeled on the metabolite composition of human blood plasma, have been shown to shift key cellular activities in several cancer cell lines. Similar effects have been reported with respect to O2 levels in cell culture. Given these observations, we investigated how media composition and O2 levels affect cellular energy metabolism and mitochondria network structure in MCF7, SaOS2, LNCaP, and Huh7 cells. Cells were cultured in physiologic (5%) or standard (18%) O2 levels, and in physiologic (Plasmax) or standard cell culture media (DMEM). We show that both O2 levels and media composition significantly affect mitochondrial abundance and network structure, concomitantly with changes in cellular bioenergetics. Extracellular acidification rate (ECAR), a proxy for glycolytic activity, was generally higher in cells cultured in DMEM while oxygen consumption rates (OCR) were lower. This effect of media on energy metabolism is an important consideration for the study of cancer drugs that target aspects of energy metabolism, including lactate dehydrogenase activity.


2019 ◽  
Vol 211 ◽  
pp. 217-226
Author(s):  
Clare Bradshaw ◽  
Dina A. Meseh ◽  
Hiba Alasawi ◽  
Ma Qiang ◽  
Pauline Snoeijs-Leijonmalm ◽  
...  

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
D. B. Mendes ◽  
F. F. Da Silva ◽  
P. M. Guarda ◽  
A. F. Almeida ◽  
D. P. de Oliveira ◽  
...  

Microbial lipases are prominent biocatalysts able to catalyze a wide variety of reactions in aqueous and nonaqueous media. In this work, filamentous fungi isolated from leaves decomposed in an aquatic environment were screened for lipase production with hydrolytic activity and esterification. Agar plates with Tween 20 and Rhodamine B were used for selection, while submerged cultures with olive oil were subsequently used to select 38 filamentous fungi. Trichoderma harzianum, Fusarium solani, Trichoderma harzianum F5, and Penicillium sp. F36 were grown in six different culture media. F. solani presented the highest lipase production (2.37 U/mL) with esterification activity of 0.07 U/mL using medium composed of (g.L−1) KH2PO4 1.00, MgSO4 H2O 1.123, and CuSO4 0.06. Supplementation of this culture medium with organic nitrogen sources increased lipase production by 461.3% using tryptone and by 419.4% using yeast extract. Among the vegetable oils from the Amazon region, degummed cotton oil induced lipase production up to 8.14 U/mL. The lipase produced by F. solani F61 has great potential to application in conventional processes and biodiesel production by transesterification of vegetable oils, as well as food industries in the production of fatty acid esters by hydrolysis and esterification.


2020 ◽  
Vol 16 (5) ◽  
pp. 757-769
Author(s):  
Muhamad Al-limoun ◽  
Haitham N. Qaralleh ◽  
Khaled M. Khleifat ◽  
Mohammed Al-Anber ◽  
Amjad Al-Tarawneh ◽  
...  

Background: A major focus of nanotechnology concerns is the expansion of the optimization of nanomaterials in purity, size and dispersity. Methods: In the current work, a two-step AgNP synthesis process was optimized at the mycelia-DI water suspension and AgNP formation reaction levels. Results: Biomass filtrate from the fungal strain Tritirachium oryzae W5H was able to reduce silver nitrate into AgNPs after a 72 h reaction, as indicated by the development of intense brown color and by UV-vis spectra. The biosynthesis ability of AgNPs was markedly better in the presence of a single carbon and nitrogen source in the culture medium compared to multiple sources of carbon and nitrogen. The optimization results of AgNP formation were indifferent between the two steps and were 20 g biomass, 40°C, pH 7.0, 96 h and 1.0 mM AgNO3. The TEM images of the prepared AgNPs illustrated the presence of 7-75 nm, monodispersed and spherical- to ovular-shaped Ag nanoparticles. Conclusion: The present work highlights the importance of investigating the process parameters by which the reductant mycelia-free filtrate was prepared. In addition, we explored the promising antibacterial action of the prepared AgNPs against bacterial infections.


Sign in / Sign up

Export Citation Format

Share Document