scholarly journals PENGARUH MACAM EKSTRAK BAHAN ORGANIK DAN ZPT TERHADAP PERTUMBUHAN PLANLET ANGGREK HASIL PERSILANGAN PADA MEDIA KULTUR

2010 ◽  
Vol 25 (1) ◽  
pp. 101
Author(s):  
Sri Hartati
Keyword(s):  
Plant Physiology ◽  
Organic Matter ◽  
Root Length ◽  
Culture Media ◽  
Soya Bean ◽  
Media Composition ◽  
Emergence Time ◽  
Two Factors

<p>This research aimed to study the effect of kinds of culture media composition added with organic matter (soya bean extract, corn extract and fish emulsion) and PGR concentration to the growth of each cross breed orchid explants. Research was conducted in Plant Physiology and Biotechnology Laboratory, Agriculture Faculty, Sebelas Maret University of Surakarta from March 2009 until the end. This research was done in Randomized Completely Design with two factors of treatment and four replications. The first factor was media and organic matter, consist of 3 levels: soya bean extract, corn extract and fish emulsion. The second factor was atonik concentration, consist of 3 levels: 0 cc/l, 0.5 cc/l and 1 cc/l. The research concluded that for the explants of crossing (Phalaeonopsis pinlong cinderela ♀ &gt;&lt; Phalaeonopsis joanekileup June” ♂) two treatment of corn extract affected significantly to the root emergence time (21.33 ) and member of root (1,92 day after planting) The treatment of fish emulsion affected significantly to the root length (2,82 cm), number of root (1,92) and produced the longest leaf. The treatment of Atonik increased the number of leaf and root.</p>

Soya-bean agglutinin induced both direct and cholecystokinin-mediated pancreatic enzyme synthesis in rats

2006 ◽  
Vol 82 (5) ◽  
pp. 645-651 ◽  
Author(s):  
J. J. Zang ◽  
D. F. Li ◽  
J. R. Wang ◽  
S. S. Tang ◽  
X. L. Li ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Culture Media ◽  
Pancreatic Enzyme ◽  
Elevated Serum ◽  
Enzyme Synthesis ◽  
Soya Bean ◽  
Pancreatic Acinar Cells ◽  
Control Group ◽  
Mucosal Cells ◽  
Two Factors

AbstractThis study was conducted to examine the relationship between soya-bean agglutinin and cholecystokinin in stimulating pancreatic enzyme synthesis in rats. In experiment 1, 30 rats were given daily gastric infusions of 0, 3·5, 7·0, 10·5, or 14·0 mg of soya-bean agglutinin (no.=6) for 14 days. Compared with the control group, soya-bean agglutinin reduced weight gains, enhanced absolute and relative dry pancreatic weights, elevated serum cholecystokinin levels, and stimulated cholecystokinin mRNA expression in the intestine (P<0·001). Pancreatic nucleic acid composition and the pancreatic activities of the enzymes amylase, trypsin and chymotrypsin all increased in response to increasing levels of soya-bean agglutinin (P<0·001). In experiment 2, duodenal cells obtained from two rats were incubated at 37°C with either saline, 100 μg/ml soya-bean agglutinin, or a mixture of 100 μg/ml soya-bean agglutinin and 10 mmol/m of the L-type, calcium channel antagonist verapamil. Soya-bean agglutinin stimulated cholecystokinin mRNA expression and cholecystokinin release from small intestinal mucosal cells, and the effect was attenuated by verapamil. In experiment 3, pancreatic acinar cells, obtained from three rats, were incubated at 37 °C with either saline, or cholecystokinin (100 fmol/ml) and soya-bean agglutinin (1000 fmol/ml), either alone or in combination. Amylase, trypsin, and chymotrypsin activities from both culture media and acini cells were stimulated by both the soya-bean agglutinin and the cholecystokinin treatments. Enzyme activities, when the two factors were incubated in combination, were intermediate to those obtained when the factors were incubated alone. This suggesting that soya-bean agglutinin may depress the action of cholecystokinin on pancreatic enzymatic activities. In summary, soya-bean agglutinin appears to stimulate pancreatic enzyme synthesis both directly and also through a cholecystokinin-mediated pathway.

scholarly journals In vitro answer of Bulgarian pepper (Capsicum annuum L.)

Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 129-136
Author(s):  
Velichka Rodeva ◽  
Stanislava Grozeva ◽  
Velichka Todorova
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Casein Hydrolysate ◽  
Basal Medium ◽  
Regeneration Ability ◽  
Stem Explants ◽  
Media Composition ◽  
Breeding Lines ◽  
High Level

Callusogenesis and regeneration ability of cotyledon and hypocotyl explants from three Bulgarian pepper varieties in MS basal medium supplemented with l-3mg/l BAP. l.0mg/1 IAA and 0.5mg/l GA3 was studied. In the different variants of culture medium was registered high level of callusogenesis and organogenesis in both type of explants from the all varieties. The highest percentage of plant-regenerants is established in cotyledon explants (from 3.3 to 18.3) in variant 3 of the culture medium containing 3mg/l BA. In the process of micropropagation by stem explants of the same studied pepper varieties the addition of the vitamins C. B12. Casein hydrolysate and Ferulic acid had a stimulation effect on the plant growth in height and rooting. In result of anther cultivation from three pepper varieties and four breeding lines the highest percentage of embryo structure formation was registered in varieties Albena and Strjama (12.0 and 13.8 respectively). The Bulgarian peppers are recalcitrant and their in vitro answer is different depending from the explants type, genotype and the culture media composition.

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals The Influence of Various Varieties and Various Liquid Organic Fertilizer to Papaya Plant Seed Growth (Carica papaya L.)

AGRIFOR ◽  
2018 ◽  
Vol 17 (1) ◽  
pp. 153
Author(s):  
Primadiyanti Arsela
Keyword(s):  
Root Length ◽  
Carica Papaya ◽  
Block Design ◽  
Organic Fertilizer ◽  
Root Number ◽  
Plant Seed ◽  
Randomized Block Design ◽  
Seed Growth ◽  
Two Factors ◽  
All Treatment

The main goal of the study was to determine the effect of various  Papaya varieties and liquid organic fertilizer on the growth of Papaya seedlings(Carica papaya L.). The research used Randomized Block Design (RAK) with two factors factorial experimental. The first factor is Variety (V) consists of 3 Papaya varieties: v1= bangkok , v2= red lady , v3= sunrise. The second factor is Fertilizer (C) consists of 3 brands: c1= Em4 10 ml, c2= Green Tonic 10ml, c3= Nasa 10ml. This study has 3 treatment levels consisting of 9 combinations and  repeated 3 times. The results of the study are treatment of various varieties (V) had significant effect on stem diameter and root number. The effect of various liquid organic fertilizer (C) had significant effect on the root length parameters. The interaction all treatment had a very significant effect on the root length and the root number parameter. 

scholarly journals Above and Belowground Growth of Corymbia maculata in a Constructed Soil: The Effect of Profile Design and Organic Amendment

2010 ◽  
Vol 36 (1) ◽  
pp. 11-17
Author(s):  
Karen Smith ◽  
Peter May ◽  
Robert White
Keyword(s):  
Organic Matter ◽  
Root Length ◽  
Organic Amendment ◽  
Root Length Density ◽  
Dry Weight ◽  
Nutrient Status ◽  
Fertilizer Treatment ◽  
Coir Fiber ◽  
Growth Responses ◽  
Profile Design

Spotted gum (Corymbia maculata (Hook.) K.D. Hill & L.A.S. Johnson), a common street tree in southern Australian cities, was used to assess growth responses to variations in profile design and organic amendment of constructed soils. Aboveground growth responses were total stem dry weight and foliar nutrient content. The belowground response was root length density. Soil profiles were constructed of sand, amended with either coir fiber, composted biosolids or composted green waste, at rates of 0, 5, 10 or 20% by volume. The profiles were either layered, with a 150 mm (6 in) organic-amended surface layer, or uniform, with amendment of the entire profile. A single fertilizer treatment was applied to all profiles. Shoot dry weight was only affected by organic matter type with the greatest growth in sand amended with composted biosolids. Foliage P and K content were affected by amendment but foliage N was not. Profile design affected root length density and distribution. Trees in uniform profiles had greater root length density, and a more uniform distribution of roots, especially with compost amendments. Above- and belowground growth increases are thought to be due to increased nutrient status resulting from organic matter mineralization.

scholarly journals The Use of Bean Sprout Extract as Supplement for the Growth of Plaintain Unti Sayang (Musa paradisiaca L.) by Tissue Culture

2014 ◽  
Vol 2 (1) ◽  
pp. 99
Author(s):  
Nirwana Jufri ◽  
Dr. Abdullah ◽  
Devi Susanti
Keyword(s):  
Tissue Culture ◽  
Root Length ◽  
Culture Media ◽  
Leaf Number ◽  
Wide Field ◽  
Root Number ◽  
Bean Sprout ◽  
Musa Paradisiaca ◽  
Tissue Culture Media ◽  
Growth Quality

One of the problems in plaintain development is on the plant breeding that has been conducted convensionally by using seedlings, so that it requires time and wide field, different treatment, and potentially brings pests and deseases. The bean sprout extract is potentially useful in improving the explant growth quality of plaintain by tissue culture. This study aims to know the influence of bean sprout extract concentration as supplement in tissue culture media upon the explant growth of plaintain Unti sayang (Musa paradisiaca L). There are 4 dosages of bean sprout extract as control. The data was analyzed by using ANOVA and was continued by using BNJ test. The result of the experiment shows that the bean sprout extract influences significatly on the plantlets height, leaf number, root length, and root number. The dosage of 100 g/l and 200 g/l bean sprout extracts provide the best result on the experimenting component of plantlet height, leaf number, root length, and root number. Meanwhile, the experimenting component of leaf number and the wet weight of the plantlets provide the best result on MS without any addition of bean sprout extract.

scholarly journals Plant physiology as affected by humified organic matter

2013 ◽  
Vol 25 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Daniel Basílio Zandonadi ◽  
Mirella Pupo Santos ◽  
Jader Galba Busato ◽  
Lázaro Eustáquio Pereira Peres ◽  
Arnoldo Rocha Façanha
Keyword(s):  
Plant Physiology ◽  
Organic Matter

Developments in in vitro technologies for swine embryo production

2004 ◽  
Vol 16 (2) ◽  
pp. 15 ◽  
Author(s):  
Matthew B. Wheeler ◽  
Sherrie G. Clark ◽  
David J. Beebe
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Physical Culture ◽  
Efficient Production ◽  
Media Composition ◽  
In Vitro Production ◽  
Considerable Research ◽  
Blastocyst Rate ◽  
Vitro Production

Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.

scholarly journals Studies on the porphobilinogen deaminase–uroporphyrinogen cosynthetase system of cultured soya-bean cells

1971 ◽  
Vol 121 (2) ◽  
pp. 327-340 ◽  
Author(s):  
Elena B. C. Llambías ◽  
Alcira M. Del C. Batlle
Keyword(s):  
Culture Media ◽  
Ph Dependence ◽  
Gel Filtration ◽  
Dicarboxylic Acids ◽  
Soya Bean ◽  
Porphobilinogen Deaminase ◽  
Starch Gel Electrophoresis ◽  
Ammonium Ions ◽  
Filtration Method ◽  
Molecular Weights

1. Porphobilinogenase was isolated and purified from soya-bean callus tissue; its components, porphobilinogen deaminase and uroporphyrinogen isomerase, were separated and purified. 2. The purified porphobilinogenase was resolved into two bands on starch-gel electrophoresis. The molecular weights of porphobilinogenase, deaminase and isomerase fractions were determined by the gel-filtration method. Porphobilinogenase activity was affected by the presence of air; uroporphyrinogens were only formed under anaerobic conditions, although substrate consumption was the same in the absence of oxygen as in its presence. 3. pH-dependence of both porphobilinogenase and deaminase was the same and a sharp optimum at pH 7.2 was obtained. Isomerase was heat-labile, but the presence of ammonium ions or porphobilinogen afforded some protection against inactivation. The action of several compounds added to the system was studied. Cysteine, thioglycollate, ammonium ions and hydroxylamine inhibited porphobilinogenase; certain concentrations of sodium and magnesium salts enhanced activity; some dicarboxylic acids and 2-methoxy-5-nitrotropone inhibited the deaminase. 4. δ-Aminolaevulate and ethionine in the culture media stimulated porphyrin synthesis and increased porphobilinogenase activity, whereas iron deficiency resulted in porphyrin accumulation. 5. The development of chlorophyll and porphobilinogenase on illumination of dark-grown callus was followed. 6. A hypothetical scheme is suggested for the enzymic synthesis of uroporphyrinogens from porphobilinogen.

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