scholarly journals A New Spectrophotometric Method for Analysis of Allopurinol in Aqueous Solutions and Pharmaceutical Preparations

2009 ◽  
Vol 6 (2) ◽  
pp. 309-314
Author(s):  
Baghdad Science Journal
Keyword(s):  
Absorption Spectrum ◽  
Aqueous Solutions ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Good Stability ◽  
Optimum Conditions ◽  
Wide Range

A new method for determination of allopurinol in microgram level depending on its ability to reduce the yellow absorption spectrum of (I-3) at maximum wavelength ( ?max 350nm) . The optimum conditions such as "concentration of reactant materials , time of sitting and order of addition were studied to get a high sensitivity ( ? = 27229 l.mole-1.cm-1) sandal sensitivity : 0.0053 µg cm-2 ,with wide range of calibration curve ( 1 – 9 µg.ml-1 ) good stability (more then24 hr.) and repeatability ( RSD % : 2.1 -2.6 % ) , the Recovery % : ( 98.17 – 100.5 % ) , the Erel % ( 0.50 -1.83 % ) and the interference's of Xanthine , Cystein , Creatinine , Urea and the Glucose in 20 , 40 , 60 fold of analyate were also studied .

scholarly journals Spectrophotometric method for simultaneous determination of valsartan and substances from the group of statins in binary mixtures

2017 ◽  
Vol 67 (4) ◽  
pp. 463-478 ◽  
Author(s):  
Mariusz Stolarczyk ◽  
Anna Apola ◽  
Anna Maślanka ◽  
Anna Kwiecień ◽  
Włodzimierz Opoka
Keyword(s):  
Simultaneous Determination ◽  
Binary Mixtures ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Derivative Spectrophotometry

Abstract Applicability of derivative spectrophotometry for the determination of valsartan in the presence of a substance from the group of statins was checked. The obtained results indicate that the proposed method may be effective by using appropriate derivatives: for valsartan and fluvastatin - D1, D2 and D3, for valsartan and pravastatin - D1 and D3, for valsartan and atorvastatin - D2 and D3. The method was characterized by high sensitivity and accuracy. Linearity was maintained in the following ranges: 9.28-32.48 mg mL-1 for valsartan, 8.16-28.56 mg mL-1 f or fluvastatin, 14.40-39.90 mg mL-1 for atorvastatin and 9.60-48.00 mg mL-1 for pravastatin. Determination coefficients were in the range of 0.989-0.999 depending on the analyte and the order of derivative. The precision of the method was high with RSD from 0.1 to 2.5 % and recovery of individual components was within the range of 100 ± 5 %. The developed method was successfully applied to the determination of valsartan combined with fluvastatin, atorvastatin and pravastatin in laboratory prepared mixtures and in pharmaceutical preparations.

scholarly journals Leishman’s Dye as a Novel Reagent in Spectrophotometric Determination of Chloramphenicol

2020 ◽  
Vol 10 (02) ◽  
pp. 250-254
Author(s):  
Jamal Sudad Raeek Othman Nabeel Sabeeh
Keyword(s):  
Spectrophotometric Method ◽  
Acidic Medium ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Sensitivity Index ◽  
Oxidizing Agent ◽  
Drug Compounds

By reviewing the literature, there is no indication concerning the use of Leishman’s dye in evaluating drug compounds by dye-color bleaching; hence, it is the first attempt to use Leishman’s dye as a novel reagent in the estimation of chloramphenicol (CAP) by an indirect spectrophotometric method in bulk and in its pharmaceutical preparations. The method includes the use of a great amount of N-bromosuccinamide (NBS) in the acidic medium as an oxidizing agent of the drug under investigation (CAP), and then using the residual of NBS for Leishman’s dye color bleaching. The absorbance has been measured at 622 nm (the maximum absorption of Leishman’s dye). A linear relationship was obtained for the Beer’s law with the concentration ranges from 10 to 250 μg/10 mL with acceptable values of molar absorptivity 0.58 × 104 L.mol-1.cm-1 and 0.055 μg.cm-2 of Sandell’s sensitivity index, which mean a high sensitivity. An approved estimation of CAP in its various pharmaceutical formulations was found.

Spectrophotometric Determination of Some Benzene Sulfonamides with 7,7,8,8-Tetracyanoquinodimethane

1989 ◽  
Vol 72 (6) ◽  
pp. 885-889 ◽  
Author(s):  
Abdel-Maboud I Mohamed
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Analytical Data ◽  
Pharmaceutical Preparations ◽  
Pure Form ◽  
Wide Range ◽  
Beer's Law ◽  
Beer’S Law ◽  
Good Agreement

Abstract A new spectrophotometric method for the determination of some unsubstituted benzene sulfonamides is presented. The method is based on the interaction of these derivatives with 7,7,8,8-tetracyanoquinodimethane at pH 9.0-9.5 to produce intense blue products. The quantitation of the products was carried out at 578 nm. Beer’s law was obeyed over a wide range of concentrations for all sulfonamide compounds studied. Optimum analytical conditions were determined, and the color produced was stable for at least 90 min at 25 °C. Analytical data for determination of sulfonamide compounds in pure form are presented together with application of the proposed method for analysis of some commercially available pharmaceutical preparations. The results are in good agreement with those obtained by official procedures.

Simultaneous Spectrophotometric Determination of Salbutamol by Coupling with Diazotized 4-Aminobenzoic acid

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Hind Hadi ◽  
Gufran Salim
Keyword(s):  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Dosage Forms ◽  
Water Soluble ◽  
Trace Determination ◽  
Aminobenzoic Acid ◽  
Optimum Conditions ◽  
Colored Product ◽  
Versus Concentration

A simple, rapid and sensitive spectrophotmetric method for trace determination of salbutamol (SAL) in aqueous solution and in pharmaceutical preparations is described. The method is based on the diazotization coupling reaction of the intended compound with 4-amino benzoic acid (ABA) in alkaline medium to form an intense orange, water soluble dye that is stable and shows maximum absorption at 410 nm. A graph of absorbance versus concentration indicates that Beer’s law is obeyed over the concentration range of 0.5-30 ppm, with a molar absorbtivity 3.76×104 L.mol-1 .cm-1 depending on the concentration of SAL. The optimum conditions and stability of the colored product have been investigated and the method was applied successfully to the determination of SAL in dosage forms.

scholarly journals Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Razieh Shakeri
Keyword(s):  
Standard Deviation ◽  
Sodium Cromoglycate ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1SCG. The limit of detection is 2.7 ng mL−1SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.

High-sensitivity thiocyanate spectrophotometric method for determination of perrhenate, an analogue of radioactive pertechnetate, under acidic condition

2018 ◽  
Vol 73 (5) ◽  
pp. 1093-1101 ◽  
Author(s):  
Hui Hu ◽  
Long-Li Sun ◽  
Yan-Ling Gao ◽  
Tian Wang ◽  
Yue-Fei Zhang ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
High Sensitivity ◽  
Acidic Condition

scholarly journals Copper-nickel oxide nanofilm modified electrode for non-enzymatic determination of glucose

2020 ◽  
Vol 10 (3) ◽  
pp. 245-255
Author(s):  
Mahsa Hasanzadeh ◽  
Zahra Hasanzadeh ◽  
Sakineh Alizadeh ◽  
Mehran Sayadi ◽  
Mojtaba Nasiri Nezhad ◽  
...  
Keyword(s):  
Modified Electrode ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Stability Loss ◽  
The Novel ◽  
Step Potential ◽  
Enzymatic Determination ◽  
Wide Range ◽  
Naoh Solution

CuxO-NiO nanocomposite film for the non-enzymatic determination of glucose was prepared by the novel modifying method. At first, anodized Cu electrode was kept in a mixture solution of CuSO4, NiSO4 and H2SO4 for 15 minutes. Then, a cathodization process with a step potential of -6 V in a mixture solution of CuSO4 and NiSO4 was initiated, generating formation of porous Cu-Ni film on the bare Cu electrode by electrodeposition assisted by the release of hydrogen bubbles acting as soft templates. Optimized conditions were determined by the experimental design software for electrodeposition process. Afterward, Cu-Ni modified electrode was scanned by cyclic voltammetry (CV) method in NaOH solution to convert Cu and Ni nanoparticles to the nano-scaled CuxO-NiO film. The electrocatalytic behavior of the novel CuxO-NiO film toward glucose oxidation was studied by CV and chronoamperometry (CHA) techniques. The calibration curve of glucose was found linear in a wide range of 0.04–5.76 mM, with a low limit of detection (LOD) of 7.3 µM (S/N = 3) and high sensitivity (1.38 mA mM-1 cm-2). The sensor showed high selectivity against some usual interfering species and high stability (loss of only 6.3 % of its performance over one month). The prepared CuxO-NiO nanofilm based sensor was successfully applied for monitoring glucose in human blood serum and urine samples.

scholarly journals Spectrophotometric investigation of the Fe(III)-disulphonate hydroquinone complex

2005 ◽  
Vol 70 (4) ◽  
pp. 651-659
Author(s):  
Mirjana Obradovic ◽  
Snezana Mitic ◽  
Snezana Tosic ◽  
A.N. Pavlovic
Keyword(s):  
Stability Constant ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Relative Stability ◽  
Time Stability ◽  
Acid Media ◽  
Influence Of Temperature ◽  
Complex Dependence ◽  
Indigo Blue

Iron(III) form an indigo-blue complex with the disulphonated product of hydroquinone (K2S2Hy) in acid media with an absorption maxima at 600 nm. The time stability of the complex, dependence of the complex absorbance on pH and the influence of temperature and solvent were followed on the basis of spectrophotometric measurements. Using the Job, mole ratio and Henry?Franck?Ostwald methods, the composition and relative stability constant of this complex, in 80 vol.% ethanol as solvent, were determined (1:1;log??293 = 3.37). A new spectrophotometric method for the determination of iron has been developed and the calibration curve is linear in the concentration range from0.65 to 6.45 ?g cm-3. The effects of foreign ions on the determination of iron were investigated in order to assess the selectivity of the method. The method was applied for the determination of Fe(III) in the natural juice of beet.

scholarly journals Spectrophotometric Determination of Epinephrine in Pharmaceutical Preparations Using Praseodymium as Mediating Metals

2011 ◽  
Vol 8 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Relative Error ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Direct Methods ◽  
Orange Yellow ◽  
Sensitive Spectrophotometric Method

A simple, accurate and sensitive spectrophotometric method for the determinaion of epinephrine is described . The method is based on the coordination of Pr (III) with epinephrine at pH 6. Absorbance of the resulting orange yellow complex is measured at 482 nm . A graph of absorbance versus concentrations shows that beer 's low is obeyed over the concentration range (1-50)mg.ml-1 of epinephrine with molar absorpitivity of ( 2.180x103 L.mol-1.cm-1 ), a sandell sensitivity of (0.084 mg.cm-2 ), a relative error of (-2.83%) , a corrolation coffecient (r= 0.9989) and recovery % ( 97.03 ± 0.75 ) depending on the concentration.This method is applied to analyse EP in several commercially available pharmaceutical preparations using direct methods .All statistical calculations are implemented via a Minitab software version 11.

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