Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

Abstract Background: Siberian sturgeon ( Acipenser baeri Brandt) and Beluga sturgeon ( Huso huso ) are two important commercial fish in China, and the feeding habits of them are very different. Diets and feeding habits are two significant factors to affect the gastrointestinal microbiota in ﬁsh. The intestinal microbiota has been reported to play a key role in nutrition and immunity. However, it is rarely reported about the relationship between the intestinal microbiota and feeding habits/diets on different Acipenseridae fish. This study is to comparative analysis of gut microbial community in Siberian sturgeon and Beluga sturgeon fed with the same diet/Beluga sturgeon fed with different diets in order to determine the effects of different feeding habits/diets on the fish intestinal microbiota. Results: According to the experimental objectives, BL and BH groups were Beluga sturgeon ( Huso huso ) fed with low fishmeal diet and high fishmeal diet, respectively. SH group represented Siberian sturgeon ( Acipenser baeri Brandt) fed with the same diet as BH group. After 16 weeks feeding trial, the intestinal microbiota was examined by 16S rRNA high-throughput sequencing technology. On the phylum level, Proteobacteria and Bacteroidetes were signiﬁcantly higher in BL group than BH group, and Cyanobacteria showed the opposite trend. Compared with BH group, Proteobacteria and Firmicutes were signiﬁcantly increased in SH group, whereas Cyanobacteria were clearly decreased. At the genus level, Pseudomonas and Citrobacter in BL group were signiﬁcantly higher comparing with BH group, while Bacillus , Luteibacter , Staphylococcus and Oceanobacillus was lower in BH group than SH group. Conclusions: Alpha and beta diversities indicated that the intestinal microﬂora were significant difference between Siberian sturgeon and Beluga sturgeon when they fed with the same diet. Meanwhile, Beluga sturgeon fed with low fishmeal diet can increase the species diversity of intestinal microbiota than it fed with high fishmeal diet. Therefore, feeding habits clearly affected the gastrointestinal microbiota of sturgeons. Moreover, the impact of changes in food on the gut microbiota of sturgeons should be taken into consideration during the process of sturgeon aquaculture.

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Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

BMC Microbiology ◽

10.1186/s12866-019-1673-6 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Guanling Xu ◽

Wei Xing ◽

Tieliang Li ◽

Min Xue ◽

Zhihong Ma ◽

...

Keyword(s):

Intestinal Microbiota ◽

Feeding Habits ◽

Siberian Sturgeon ◽

Huso Huso ◽

Gastrointestinal Microbiota ◽

Acipenser Baeri ◽

Significant Difference ◽

Sh Group ◽

The Impact ◽

Beluga Sturgeon

Abstract Background Siberian sturgeon (Acipenser baeri Brandt) and Beluga sturgeon (Huso huso) are two important commercial fish in China, and the feeding habits of them are very different. Diets and feeding habits are two significant factors to affect the gastrointestinal microbiota in fish. The intestinal microbiota has been reported to play a key role in nutrition and immunity. However, it is rarely reported about the relationship between the intestinal microbiota and feeding habits/diets on different Acipenseridae fish. This study is to comparative analysis of gut microbial community in Siberian sturgeon and Beluga sturgeon fed with the same diet/Beluga sturgeon fed with different diets in order to determine the effects of different feeding habits/diets on the fish intestinal microbiota. Results According to the experimental objectives, BL and BH groups were Beluga sturgeon (Huso huso) fed with low fishmeal diet and high fishmeal diet, respectively. SH group represented Siberian sturgeon (Acipenser baeri Brandt) fed with the same diet as BH group. After 16 weeks feeding trial, the intestinal microbiota was examined by 16S rRNA high-throughput sequencing technology. On the phylum level, Proteobacteria and Bacteroidetes were significantly higher in BL group than BH group, and Cyanobacteria showed the opposite trend. Compared with BH group, Proteobacteria and Firmicutes were significantly increased in SH group, whereas Cyanobacteria were clearly decreased. At the genus level, Pseudomonas and Citrobacter in BL group were significantly higher comparing with BH group, while Bacillus, Luteibacter, Staphylococcus and Oceanobacillus was lower in BH group than SH group. Conclusions Alpha and beta diversities indicated that the intestinal microflora were significant difference between Siberian sturgeon and Beluga sturgeon when they fed with the same diet. Meanwhile, Beluga sturgeon fed with low fishmeal diet can increase the species diversity of intestinal microbiota than it fed high fishmeal diet. Therefore, feeding habits clearly affected the gastrointestinal microbiota of sturgeons. Moreover, the impact of changes in food on the gut microbiota of sturgeons should be taken into consideration during the process of sturgeon aquaculture.

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Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

10.21203/rs.2.13265/v3 ◽

2019 ◽

Author(s):

Guanling Xu ◽

Wei Xing ◽

Tieliang Li ◽

Min Xue ◽

Zhihong Ma ◽

...

Keyword(s):

Intestinal Microbiota ◽

Feeding Habits ◽

Siberian Sturgeon ◽

Huso Huso ◽

Gastrointestinal Microbiota ◽

Acipenser Baeri ◽

Significant Difference ◽

Sh Group ◽

The Impact ◽

Beluga Sturgeon

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Comparative analysis of intestinal microbiota in two different Acipenseridae fish fed with the same diet/the same fish fed with two different diets

10.21203/rs.2.13265/v1 ◽

2019 ◽

Author(s):

Guanling Xu ◽

Wei Xing ◽

Tieliang Li ◽

Min Xue ◽

Zhihong Ma ◽

...

Keyword(s):

Comparative Analysis ◽

Intestinal Microbiota ◽

Intestinal Microflora ◽

Feeding Habits ◽

Siberian Sturgeon ◽

Huso Huso ◽

Gastrointestinal Microbiota ◽

Acipenser Baeri ◽

Sh Group ◽

Beluga Sturgeon

Abstract Background: Siberian sturgeon (Acipenser baeri Brandt) and Beluga sturgeon (Huso huso) are two important commercial fish in China, and the feeding habits of them are very different. Diets and feeding habits are two significant factors to affect the gastrointestinal microbiota in ﬁsh. The intestinal microbiota has been reported to play a key role in nutrition and immunity. However, it is rarely reported about the relationship between the intestinal microbiota and feeding habits/diets on different Acipenseridae fish. This study is to comparative analysis of gut microbial community in two different Acipenseridae fish fed with the same diet/the same fish with different diets in order to realize the effects of different feeding habits/diets on the intestinal microﬂora of fish. Results: According to the experimental objectives, BL and BH groups were Beluga sturgeon (Huso huso) fed with low fishmeal diet and high fishmeal diet, respectively. SH group represented Siberian sturgeon (Acipenser baeri Brandt) fed with the same diet as BH group. After 16 weeks feeding trial, the intestinal microbiota was examined by 16S rRNA high-throughput sequencing technology. On the phylum level, Proteobacteria and Bacteroidetes were signiﬁcantly higher in BL group than BH group, and Cyanobacteria showed the opposite trend. Compared with BH group, Proteobacteria and Firmicutes were signiﬁcantly increased in SH group, whereas Cyanobacteria were clearly decreased. At the genus level, Pseudomonas and Citrobacter in BL group were signiﬁcantly higher comparing with BH group, while Bacillus, Luteibacter, Staphylococcus and Oceanobacillus was lower in BH group than SH group. Conclusions: Alpha and beta diversities indicated that the intestinal microﬂora were significant difference between Siberian sturgeon and Beluga sturgeon when they fed with the same diet. Meanwhile, Beluga sturgeon fed with low fishmeal diet can increase the species diversity of intestinal microbiota than it fed high fishmeal diet. Therefore, feeding habits clearly affected the gastrointestinal microbiota of sturgeons. Moreover, the impact of changes in food on the gut microbiota of sturgeons should be taken into consideration during the process of sturgeon aquaculture.

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In VitroCulture Conditions for Maintaining a Complex Population of Human Gastrointestinal Tract Microbiota

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/838040 ◽

2011 ◽

Vol 2011 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Bong-Soo Kim ◽

Jong Nam Kim ◽

Carl E. Cerniglia

Keyword(s):

Gastrointestinal Tract ◽

Bacterial Community ◽

Intestinal Microbiota ◽

Brain Heart Infusion ◽

Culture Conditions ◽

Human Gastrointestinal Tract ◽

Gastrointestinal Microbiota ◽

Wide Range ◽

The Impact

A stable intestinal microbiota is important in maintaining human physiology and health. Although there have been a number of studies usingin vitroandin vivoapproaches to determine the impact of diet and xenobiotics on intestinal microbiota, there is no consensus for the bestin vitroculture conditions for growth of the human gastrointestinal microbiota. To investigate the dynamics and activities of intestinal microbiota, it is important for the culture conditions to support the growth of a wide range of intestinal bacteria and maintain a complex microbial community representative of the human gastrointestinal tract. Here, we compared the bacterial community in three culture media: brain heart infusion broth and high- and low-carbohydrate medium with different growth supplements. The bacterial community was analyzed using denaturing gradient gel electrophoresis (DGGE), pyrosequencing and real-time PCR. Based on the molecular analysis, this study indicated that the 3% fecal inoculum in low-concentration carbohydrate medium with 1% autoclaved fecal supernatant provided enhanced growth conditions to conductin vitrostudies representative of the human intestinal microbiota.

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Cannibalism as a feeding strategy for mantis shrimp Oratosquilla oratoria (De Haan, 1844) in the Tianjin coastal zone of Bohai Bay

10.1101/740100 ◽

2019 ◽

Author(s):

Qi-Kang Bo ◽

Yun-Zhao Lu ◽

Hui-Jing Mi ◽

Yan-Guang Yu ◽

De-Xian Gu ◽

...

Keyword(s):

Coastal Zone ◽

Trophic Ecology ◽

Feeding Habits ◽

Feeding Strategy ◽

Feeding Activity ◽

Bohai Bay ◽

Mantis Shrimp ◽

Oratosquilla Oratoria ◽

Significant Difference ◽

The Impact

AbstractA representative semi-enclosed bay of China, Bohai Bay has experienced severe interference in recent decades and is under threat from rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify the prey consumed by O. oratoria through the separation of stomach contents and to describe its trophic ecology during maturation, from March to July, in the Tianjin coastal zone of Bohai Bay. A total of 594 specimens were collected and 347 (58.59%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding activity of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. And the feeding activities of O. oratoria were consistent over the study months. A total of 207 prey items yielded 231 readable sequences and 24 different taxa were identified. Prey detected in O. oratoria consisted mainly of crustaceans, which accounted for 71.86 % of the clones detected; 16.02% corresponded to fishes, 8.23% corresponded to mollusks and the remaining 3.90% corresponded to other marine organisms. Cannibalism (occured frequently, 69.08%) in this study was noticeably higher than that seen in previous studies and confirmed that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay. The ecological environment in Bohai Bay has been affected by anthropogenic activities and the macrofaunal biodiversity and abundance have noticeably declined, which might make the food scarce for the mantis shrimp O. oratoria. Then, the starvation obviously increased cannibalistic tendencies.

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Clinical and microbiological aspects of the use of Lactobacillus rhamnosus PL1 strains in proctological patients with symptoms of chronic proctitis

Polish Journal of Surgery ◽

10.5604/01.3001.0010.1083 ◽

2017 ◽

Vol 89 (3) ◽

pp. 16-22

Author(s):

K. Borycka-Kiciak ◽

M. Strus ◽

P. Pietrzak ◽

K. Wawiernia ◽

D. Mikołajczyk ◽

...

Keyword(s):

Intestinal Microbiota ◽

Bacterial Flora ◽

Lactobacillus Rhamnosus ◽

Underlying Disease ◽

Entire Study ◽

Qualitative And Quantitative ◽

Significant Difference ◽

Probiotic Lactobacillus ◽

Quantitative Changes ◽

The Impact

Recurrent proctitis and the symptoms associated therewith pose significant clinical problem in proctological patients. The objective of this study was to assess the impact of the probiotic Lactobacillus rhamnosus PL1 strain on the clinical presentation and composition of intestinal microbiota in patients with symptoms of proctitis in the course of hemorrhoidal disease and diverticulosis. Material consisted of 24 patients in whom no complete clinical improvement could be obtained after the treatment of the underlying disease. Subject to the assessment was the presence and the intensity of clinical symptoms as well as qualitative and quantitative changes in the composition of bacterial flora detected in the stool before, during and after a 9-week supplementation with the probiotic Lactobacillus rhamnosus PL1 strain. Results: In the entire study group, the intensity of pain after 12 weeks was significantly lower (p=0,.011) compared to baseline; the intensity of flatulence and abdominal discomfort was reduced significantly as early as after 3 weeks, with the difference reaching a highly significant level after 12 weeks (pP<0,.0001). No significant difference was observed in the frequency of the reported episodes of diarrhea, constipation, as well as itching and burning in the anal region. As early as after 3 weeks of supplementation with the probiotic L. rhamnosus PL1 strain, significant qualitative and quantitative changes were observed in the composition of intestinal microbiota; the changes differed depending on the underlying disease. An increase in the total counts of the bacteria of Lactobacillus genus, particularly L. rhamnosus PL1 strain was observed regardless of the underlying disease. Conclusion: Tthe probiotic Lactobacillus rhamnosus PL1 strain appears to be useful in restoring appropriate ratios of bacterial populations in patients presenting with symptoms of proctitis in the course of the treatment of certain diseases of the lower gastrointestinal tract.

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Effects of Antibiotics on the Intestinal Microbiota of Mice

Antibiotics ◽

10.3390/antibiotics9040191 ◽

2020 ◽

Vol 9 (4) ◽

pp. 191 ◽

Cited By ~ 1

Author(s):

Frederik Boetius Hertz ◽

Andries E. Budding ◽

Malieka van der Lugt-Degen ◽

Paul H. Savelkoul ◽

Anders Løbner-Olesen ◽

...

Keyword(s):

Intestinal Microbiota ◽

Bacterial Load ◽

Antibiotic Usage ◽

Resistant Bacteria ◽

Colonization Resistance ◽

Gastrointestinal Microbiota ◽

E Coli ◽

The Impact ◽

Human And Mouse

Studies on human and mouse gastrointestinal microbiota have correlated the composition of the microbiota to a variety of diseases, as well as proved it vital to prevent colonization with resistant bacteria, a phenomenon known as colonization resistance. Antibiotics dramatically modify the gut community and there are examples of how antibiotic usage lead to colonization with resistant bacteria [e.g., dicloxacillin usage selecting for ESBL-producing E. coli carriage], as shown by Hertz et al. Here, we investigated the impact of five antibiotics [cefotaxime, cefuroxime, dicloxacillin, clindamycin, and ciprofloxacin] on the intestinal microbiota in mice. Five different antibiotics were each given to groups of five mice. The intestinal microbiotas were profiled by use of the IS-pro analysis; a 16S–23S rDNA interspace [IS]-region-based profiling method. For the mice receiving dicloxacillin and clindamycin, we observed dramatic shifts in dominating phyla from day 1 to day 5. Of note, diversity increased, but overall bacterial load decreased. For ciprofloxacin, cefotaxime, and cefuroxime there were few overall changes. We speculate that antibiotics with efficacy against the abundant anaerobes in the gut, particularly Bacteroidetes, can in fact be selected for resistant bacteria, disregarding the spectrum of activity.

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The impact of dietary DHA/EPA ratio on spawning performance, egg and offspring quality in Siberian sturgeon (Acipenser baeri)

Aquaculture ◽

10.1016/j.aquaculture.2014.11.036 ◽

2015 ◽

Vol 437 ◽

pp. 140-145 ◽

Cited By ~ 30

Author(s):

Lin Luo ◽

Lichuan Ai ◽

Tieliang Li ◽

Min Xue ◽

Jia Wang ◽

...

Keyword(s):

Siberian Sturgeon ◽

Offspring Quality ◽

Acipenser Baeri ◽

The Impact

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Measuring Impact of Stress in Sandwich Generation Caring for Demented Parents

GeroPsych ◽

10.1024/1662-9647/a000114 ◽

2014 ◽

Vol 27 (4) ◽

pp. 171-179 ◽

Cited By ~ 6

Author(s):

Laurence M. Solberg ◽

Lauren B. Solberg ◽

Emily N. Peterson

Keyword(s):

Factor Analysis ◽

Exploratory Factor Analysis ◽

Social Worker ◽

Resource Availability ◽

Well Being ◽

Sandwich Generation ◽

Item Questionnaire ◽

Significant Difference ◽

Adult Caregivers ◽

The Impact

Stress in caregivers may affect the healthcare recipients receive. We examined the impact of stress experienced by 45 adult caregivers of their elderly demented parents. The participants completed a 32-item questionnaire about the impact of experienced stress. The questionnaire also asked about interventions that might help to reduce the impact of stress. After exploratory factor analysis, we reduced the 32-item questionnaire to 13 items. Results indicated that caregivers experienced stress, anxiety, and sadness. Also, emotional, but not financial or professional, well-being was significantly impacted. There was no significant difference between the impact of caregiver stress on members from the sandwich generation and those from the nonsandwich generation. Meeting with a social worker for resource availability was identified most frequently as a potentially helpful intervention for coping with the impact of stress.

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Utilizing next-generation sequencing to identify prey DNA in western North Atlantic grey seal Halichoerus grypus diet

Marine Ecology Progress Series ◽

10.3354/meps13520 ◽

2020 ◽

Vol 655 ◽

pp. 227-240

Author(s):

KR Flanders ◽

ZH Olson ◽

KA Ono

Keyword(s):

Next Generation Sequencing ◽

Feeding Habits ◽

Atlantic Menhaden ◽

Halichoerus Grypus ◽

Frequency Of Occurrence ◽

Grey Seal ◽

Next Generation ◽

Significant Difference ◽

Generation Sequencing ◽

Seal Diet

Increasing grey seal Halichoerus grypus abundance in coastal New England is leading to social, political, economic, and ecological controversies. Central to these issues is the foraging ecology and diet composition of the seals. We studied grey seal feeding habits through next-generation sequencing of prey DNA using 16S amplicons from seal scat (n = 74) collected from a breeding colony on Monomoy Island in Massachusetts, USA, and report frequency of occurrence and relative read abundance. We also assigned seal sex to scat samples using a revised PCR assay. In contrast to current understanding of grey seal diet from hard parts and fatty acid analysis, we found no significant difference between male and female diet measured by alpha and beta diversity. Overall, we detected 24 prey groups, 18 of which resolved to species. Sand lance Ammodytes spp. were the most frequently consumed prey group, with a frequency of occurrence (FO) of 97.3%, consistent with previous studies, but Atlantic menhaden Brevoortia tyrannus, the second most frequently consumed species (FO = 60.8%), has not previously been documented in US grey seal diet. Our results suggest that a metabarcoding approach to seal food habits can yield important new ecological insights, but that traditional hard parts analysis does not underestimate consumption of Atlantic cod Gadus morhua (FO = 6.7%, Gadidae spp.) and salmon Salmo salar (FO = 0%), 2 particularly valuable species of concern.

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