scholarly journals Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

2019 ◽  
Author(s):  
Shawon Ahmmed ◽  
Md. Abdullah-Al-Kamran Khan ◽  
Md. Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Abul B.M.M.K. Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Penaeus Monodon ◽  
Antibiotic Resistance Genes ◽  
Development Stage ◽  
Secretion System ◽  
Economic Losses ◽  
Emergence Pattern ◽  
Toxin Genes ◽  
South West ◽  
Evolutionary Features

Abstract Background Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus, carrying the pirA and pirB toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence the whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp (Peneaus monodon) of the south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh, AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type III secretion system-1 (T3SS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pirA and pirB. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline and macrolide groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic-resistant V. parahaemolyticus isolates between production sites, and the identification of candidate strains for the production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

scholarly journals Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Shawon Ahmmed ◽  
Md. Abdullah-Al-Kamran Khan ◽  
Md. Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Abul Bashar Mir Md. Khademul Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Penaeus Monodon ◽  
Antibiotic Resistance Genes ◽  
Development Stage ◽  
Secretion System ◽  
Economic Losses ◽  
Emergence Pattern ◽  
Toxin Genes ◽  
South West ◽  
Evolutionary Features

Abstract Background Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus, carrying the pirA and pirB toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence the whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp (Peneaus monodon) of the south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh, AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type III secretion system-1 (T3SS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~ 69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pirA and pirB. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline and macrolide groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic-resistant V. parahaemolyticus isolates between production sites, and the identification of candidate strains for the production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

scholarly journals Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

2019 ◽  
Author(s):  
Shawon Ahmmed ◽  
Md. Abdullah-Al-Kamran Khan ◽  
Md. Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Abul B.M.M.K. Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Early Stage ◽  
Penaeus Monodon ◽  
Antibiotic Resistance Genes ◽  
Secretion System ◽  
Economic Losses ◽  
Emergence Pattern ◽  
Toxin Genes ◽  
South West ◽  
Evolutionary Features

Abstract Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus , carrying the pir A and pir B toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence the whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp ( Peneaus monodon ) of south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh , AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type III secretion system-1 (T3SS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pir A and pir B. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline and macrolide groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic-resistant V. parahaemolyticus isolates between production sites, and the identification of candidate strains for the production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

scholarly journals Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

2019 ◽  
Author(s):  
Shawon Ahmmed ◽  
Md. Abdullah-Al-Kamran Khan ◽  
Md. Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Abul B.M.M.K. Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Early Stage ◽  
Penaeus Monodon ◽  
Antibiotic Resistance Genes ◽  
Secretion System ◽  
Economic Losses ◽  
Emergence Pattern ◽  
Toxin Genes ◽  
South West ◽  
Evolutionary Features

Abstract Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus, carrying the pirA and pirB toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence the whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp (Peneaus monodon) of south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh, AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type III secretion system-1 (T3SS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pirA and pirB. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline and macrolide groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic-resistant V. parahaemolyticus isolates between production sites, and the identification of candidate strains for the production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

scholarly journals Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

2019 ◽  
Author(s):  
Shawon Ahmmed ◽  
Md. Abdullah-Al-Kamran Khan ◽  
Md. Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Abul B.M.M.K. Islam ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Parahaemolyticus ◽  
Early Stage ◽  
Antibiotic Resistance Genes ◽  
Secretion System ◽  
Economic Losses ◽  
Emergence Pattern ◽  
Toxin Genes ◽  
South West ◽  
Evolutionary Features

Abstract Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) causing great economic losses, since it first outbreak in southeast China in 2009. Vibrio parahaemolyticus , carrying the pir A and pir B toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp ( Peneaus monodon ) of south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh , AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of total 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type three secretion system-1 (TTSS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pir A and pir B. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline, macrolide and cephalosporin groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic resistance V. parahaemolyticus isolates between production sites, and the identification of candidate strains for production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

scholarly journals Molecular characterization of acute hepatopancreatic necrosis disease causing Vibrio parahaemolyticus strains in cultured shrimp Penaeus monodon in south‐west farming region of Bangladesh

2018 ◽  
Vol 27 (1) ◽  
pp. 57-68 ◽  
Author(s):  
Md Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Mst Khadiza Begum ◽  
Tahsin Khan ◽  
Mihir Lal Saha ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Penaeus Monodon ◽  
16S Rrna Gene Sequencing ◽  
Molecular Techniques ◽  
Shrimp Farming ◽  
Economic Losses ◽  
Rrna Gene ◽  
South West ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Positive Results

Acute hepatopancreatic necrosis disease (AHPND) is an emerging shrimp disease caused by strains of Vibrio parahaemolyticus containing a unique virulent plasmid, responsible for substantial economic losses since 2009; caused up to 100% mortality in farmed shrimp Penaeus monodon. The purpose of this study was to isolate and identify the pathogenic strain of V. parahaemolyticus causing AHPND in cultured shrimp (Penaeus monodon) using classical and molecular techniques. Samples were collected from three different locations of south-west shrimp farming regions of Bangladesh viz. Sadar Upazilla of Satkhira; Mongla and Morrelganj under Bagerhat district. In this study, three selective media were used for primary isolation of V. parahaemolyticus. Among 46 primary isolates, 18 representative isolates were checked for the species-specific detection of V. parahaemolyticus using ldh primers and all of them were found to be positive. 16S rRNA gene sequencing were used to further confirm the isolates as V. parahaemolyticus. tdh primer was used to check human pathogenicity but all 18 isolates showed negative result. The isolates were further characterized to check their AHPND positivity using AP3 and AP4 primers. Ten isolates showed positive results for AP3 (55.56%) and 9 showed positive results for AP4 (50%) which indicated that the isolates were AHPND positive. This study also reported that all AHPND positive strains were resistant to the antibiotic gentamycin but sensitive to chloramphenicol, nalidixic acid, nitrofurantoin and tetracycline. The findings of this study will help the shrimp farmers and policy makers to take proper biosecurity measures to protect shrimps from AHPND and thereby sustain the shrimp production in Bangladesh. Dhaka Univ. J. Biol. Sci. 27(1): 57-68, 2018 (January)

scholarly journals Characterization of Pathogenic Vibrio parahaemolyticus Isolated From Fish Aquaculture of the Southwest Coastal Area of Bangladesh

2021 ◽  
Vol 12 ◽  
Author(s):  
Abu Baker Siddique ◽  
M. Moniruzzaman ◽  
Sobur Ali ◽  
Md. Nayem Dewan ◽  
Mohammad Rafiqul Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pearson Correlation ◽  
Penaeus Monodon ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Economic Losses ◽  
Ileal Loop ◽  
Virulence Potential ◽  
Pathogenic Vibrio

Vibrio parahaemolyticus is a major foodborne pathogen responsible for significant economic losses in aquaculture and a threat to human health. Here, we explored the incidence, virulence potential, and diversity of V. parahaemolyticus isolates from aquaculture farms in Bangladesh. We examined a total of 216 water, sediment, Oreochromis niloticus (tilapia), Labeo rohita (rui), and Penaeus monodon (shrimp) samples from the aquaculture system where 60.2% (130/216) samples were positive for V. parahaemolyticus. Furthermore, we identified 323 V. parahaemolyticus strains from contaminated samples, 17 of which were found positive for trh, a virulence gene. Four isolates out of the 17 obtained were able to accumulate fluid in the rabbit ileal loop assay. The correlation between the contamination of V. parahaemolyticus and environmental factors was determined by Pearson correlation. The temperature and salinity were significantly correlated (positive) with the incidence of V. parahaemolyticus. Most of the pathogenic isolates (94.1%) were found resistant to ampicillin and amoxicillin. O8: KUT was the predominant serotype of the potentially pathogenic isolates. ERIC-PCR reveals genetic variation and relatedness among the pathogenic isolates. Therefore, this region-specific study establishes the incidence of potential infection with V. parahaemolyticus from the consumption of tilapia, rui, and shrimp raised in farms in Satkhira, Bangladesh, and the basis for developing strategies to reduce the risk for diseases and economic burden.

scholarly journals The infection of Vibrio parahaemolyticus in shrimp and human

2018 ◽  
Vol 1 (1) ◽  
pp. 44 ◽  
Author(s):  
Rian Ka Praja
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Animal Health ◽  
Alternative Therapy ◽  
Penaeus Monodon ◽  
Early Mortality ◽  
Economic Losses ◽  
Early Mortality Syndrome ◽  
Thermostable Direct Hemolysin ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Very High

<p class="15" align="justify"><em>Vibrio parahaemolyticus</em> is an aquatic zoonotic agent that can threaten human and aquaculture animal health. Humans can be infected by consuming contaminated raw seafood or wound-related infections. Generally infection of <em>V. parahemolyticus</em> is orally transmitted and causes gastroenteritis in humans while in aquaculture animals especially shrimp can cause Acute Hepatopancreatic Necrosis Disease (AHPND) or Early Mortality Syndrome (EMS) with a very high mortality rate and cause economic losses. Shrimp species susceptible to infection are <em>Litopenaeus vannamei, Penaeus monodon,</em> and <em>P. chinensis</em>. <em>V. parahaemolyticus</em> produces several toxins in human disease such as thermostable direct hemolysin (TDH), TDH-related haemolysin (TRH), and thermolabile hemolysin (TLH). Meanwhile, Photorabdus insect-related (Pir) toxins consisting of PirA<sup>vp</sup> and PirB<sup>vp</sup> are the toxins associated with AHPND in shrimp. The genes that encode the toxin are used as targets to diagnose <em>V. parahaemolyticus</em> pathogens molecularly. Until now the treatment of <em>V. parahaemolyticus</em> infection is using antibiotics and fluid therapy, but there were <em>V. parahaemolyticus</em> isolates from aquaculture that have been resistant to antibiotics so that the use of antibiotics in aquaculture must be controlled and the use of alternative therapy are very important to be developed to control <em>V. parahaemolyticus</em> infection.</p><p class="15" align="justify"> </p><p>Keywords: <em>V. parahaemolyticus</em>, zoonotic, gastroenteritis, Acute Hepatopancreatic Necrosis Disease (AHPND), Early Mortality Syndrome (EMS).</p>

scholarly journals Immune and bacterial toxin genes expression in different giant tiger prawn, penaeus monodon post-larvae stages following AHPND-causing strain of vibrio parahaemolyticus challenge

2020 ◽  
Vol 16 ◽  
pp. 100248
Author(s):  
Zulaikha Mat Deris ◽  
Shumpei Iehata ◽  
Mhd Ikhwanuddin ◽  
Mohd Badrul Mohamad Khairul Sahimi ◽  
Thinh Dinh Do ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Penaeus Monodon ◽  
Bacterial Toxin ◽  
Toxin Genes ◽  
Genes Expression ◽  
Tiger Prawn

scholarly journals Draft Genome Sequence of a Vibrio parahaemolyticus Strain, KS17.S5-1, with Multiple Antibiotic Resistance Genes, Which Causes Acute Hepatopancreatic Necrosis Disease in Penaeus monodon in the West Coast of Peninsular Malaysia

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Sridevi Devadas ◽  
Subha Bhassu ◽  
Tze Chiew Christie Soo ◽  
Suhaina Nashath Mohamed Iqbal ◽  
Fatimah M. Yusoff ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Vibrio Parahaemolyticus ◽  
Draft Genome ◽  
Penaeus Monodon ◽  
Antibiotic Resistance Genes ◽  
Peninsular Malaysia ◽  
Draft Genome Sequence ◽  
Parahaemolyticus Strain ◽  
Content Type ◽  
Acute Hepatopancreatic Necrosis Disease

We report the first draft genome sequence of a Vibrio parahaemolyticus strain (Vp AHPND), which causes acute hepatopancreatic necrosis disease (AHPND) in Penaeus monodon. The strain has a pVA1-like plasmid carrying pirAvp and pirBvp genes.

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

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