scholarly journals Comparative Study of Imaging and Pathology of Sacroiliac Joint in Normal Rats Based on IVIM-DWI and DCE-MRI

2020 ◽  
Author(s):  
Jian Qin ◽  
Qianqian Yao ◽  
Xubo Ge ◽  
Jianzhong Zhu ◽  
Zhaoliang Yin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sacroiliac Joint ◽  
Age Groups ◽  
Joint Space ◽  
Iliac Bone ◽  
Mri Findings ◽  
Dce Mri ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Special Value

Abstract Background : There are few reports describing the relationship between functional MRI findings and pathology of normal sacroiliac joint (SIJ). Furthermore, the pathology of SIJ is difficult to access, so animal models have special value. Aims:We undertook this study to explore the changes of parameters of Intravoxel Incoherent Motion Diffusion Weighted Imaging(IVIM-DWI), Dynamic Contrast Enhanced Magnetic Resonance Imaging(DCE-MRI) and compare them with pathology in the SIJ of normal rats in different ages.Methods: Thirty 7 week-old male wistar rats were included in the study. The parameters of IVIM-DWI and DCE-MRI in bone marrow and the joint space of SIJ were measured respectively at 8, 13, 18, 23, 28 and 33 weeks, then histological specimen of the SIJ were examined with light microscopy. One-way ANOVA were used for statistical analysis. Results: The values of D in the sacral and iliac bone marrow of normal rats in different age groups decreased with the increase of age, and ANOVA indicated a significant difference in D values among different age groups(P＜0.005). The normal values of D*, f, Fenh(%), Senh(%/s) in the sacral bone marrow, the iliac bone marrow and in the joint space in SIJ of normal rats were obtained. In six groups of rats with different weeks of age, the pathology of the SIJ surface was smooth and clear, the cartilage cells were intact, and no thickening or pannus formation was found. Conclusions: The range of IVIM-DWI and DCE-MRI parameters of the sacral and iliac bone marrow and of the synovial area of the joint space in normal rats were obtained. It provides a basis for further clinical study of SIJ lesions.

scholarly journals Comparative Study of Imaging and Histology of Sacroiliac Joint in Normal Rats Based on IVIM-DWI and DCE-MRI

2020 ◽  
Author(s):  
Jian Qin ◽  
Qianqian Yao ◽  
Xubo Ge ◽  
Jianzhong Zhu ◽  
Zhaoliang Yin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Animal Models ◽  
Sacroiliac Joint ◽  
Age Groups ◽  
Joint Space ◽  
Iliac Bone ◽  
Future Research ◽  
Mri Findings ◽  
Dce Mri ◽  
Significant Difference

Abstract Background : There are few reports describing the relationship between functional MRI findings and histology of normal sacroiliac joint (SIJ). Furthermore, the histology of SIJ is difficult to access, so animal models have special value. Aims:We undertook this study to explore the changes of parameters of Intravoxel Incoherent Motion Diffusion Weighted Imaging(IVIM-DWI), Dynamic Contrast Enhanced Magnetic Resonance Imaging(DCE-MRI) and compare them with histology in the SIJ of normal rats in different ages.Methods: Thirty 7 week-old male wistar rats were included in the study. The parameters of IVIM-DWI and DCE-MRI in bone marrow and the joint space of SIJ were measured respectively at 8, 13, 18, 23, 28 and 33 weeks, then histological specimen of the SIJ were examined with light microscopy. One-way ANOVA were used for statistical analysis. Results: The values of D in the sacral and iliac bone marrow of normal rats in different age groups decreased with the increase of age, and ANOVA indicated a significant difference in D values among different age groups(P＜0.005). The normal values of D*, f, Fenh(%), Senh(%/s) in the sacral bone marrow, the iliac bone marrow and in the joint space in SIJ of normal rats were obtained. In six groups of rats with different weeks of age, the histology of the SIJ surface was smooth and clear, the cartilage cells were intact, and no thickening or pannus formation was found. Conclusions: The range of IVIM-DWI and DCE-MRI parameters of the sacral and iliac bone marrow and of the synovial area of the joint space in normal rats were obtained. These parameters in normal rats can be used in future research to compare them to the same parameters in animal models or patients with SIJ diseases.This study can be a guide for future research in SIJ diseases.

scholarly journals Comparative Study of Imaging and Pathology of Sacroiliac Joint in Normal Rats Based on IVIM-DWI and DCE-MRI

2019 ◽  
Author(s):  
Jian Qin ◽  
Qianqian Yao ◽  
Xubo Ge ◽  
Jianzhong Zhu ◽  
Zhaoliang Yin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sacroiliac Joint ◽  
Synovial Membrane ◽  
Age Groups ◽  
Joint Space ◽  
Mri Findings ◽  
Dce Mri ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Relationship

Abstract Background There are few reports describing the relationship between functional MRI findings and pathology of normal sacroiliac joint (SIJ). Furthermore, the pathology of SIJ is difficult to access, so animal models have special value.Aims We undertook this study to explore the relationship between parameters of Intravoxel incoherent motion(IVIM-DWI), Dynamic contract enhance(DCE-MRI) and pathology in SIJ of rats in different ages.Methods Thirty 7 week-old male wistar rats were included in the study. The parameters of IVIM-DWI and DCE-MRI in bone marrow and synovial membrane of SIJ were measured respectively at 8th, 13th, 18th, 23th, 28th and 33th weeks, then the pathological features of SIJ were gotten. One-way ANOVA were used for statistical analysis.Results The values of D in the sacrum, ilium marrow of normal rats in different age groups decreased with the increase of age, and ANOVA indicated a significant difference in D values among different age groups( P= 0.000). The normal values of D*, f, Fenh%, Senh(s -1 ) in the sacrum, ilium marrow, and the synovial membrane in SIJ of normal rats were obtained. In six groups of rats with different weeks of age, the pathology of SIJ surface was smooth and clear, the cartilage cells were intact, and no thickening or pannus formation was found.Conclusions The range of IVIM-DWI and DCE-MRI parameters of sacrum, ilium bone marrow and synovial area of joint space in normal rats were obtained. It provides a basis for further clinical study of SIJ lesions.

scholarly journals Comparative Study of Imaging and Histology of Sacroiliac Joint in Normal Rats Based on IVIM-DWI and DCE-MRI

2020 ◽  
Author(s):  
Jian Qin ◽  
Qianqian Yao ◽  
Xubo Ge ◽  
Jianzhong Zhu ◽  
Zhaoliang Yin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Animal Models ◽  
Sacroiliac Joint ◽  
Joint Space ◽  
Iliac Bone ◽  
Future Research ◽  
Dce Mri ◽  
Different Ages ◽  
D Values ◽  
One Way Anova

Abstract Background: Currently, few studies have described the relationship between functional MRI findings and histology of normal sacroiliac joint (SIJ). Besides, due to the difficulties in access to SIJ, authentic animal models are important in providing opportunities for quantitative parameter extraction on imaging.Aims: This study aimed at exploring the parameters of Intravoxel Incoherent Motion Diffusion-Weighted Imaging (IVIM-DWI) and Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) and comparing them with the histology of the SIJ in normal rats with different ages.Methods: A total of thirty 7-week-old male Wistar rats were included in the study. The parameters of IVIM-DWI and DCE-MRI in the bone marrow and the joint space of SIJ were measured at 8, 13, 18, 23, 28, and 33 weeks. The histological analysis of the SIJ was examined using light microscopy. One-way ANOVA was used for statistical analysis. Results: The D values in the sacral and iliac bone marrow of normal rats decreased with an increase in age. One-way ANOVA analysis indicated a significant difference in D values in different age groups (P＜0.005). The normal values of D*, f, Fenh (%), Senh (%/s) in the sacral bone marrow, the iliac bone marrow, and the joint space in SIJ of normal rats were obtained. The results showed that in the six groups of rats of different ages, the histology of the SIJ surface was smooth and clear, the cartilage cells were intact, and no thickening or pannus formation was observed. Conclusions: This study obtained the IVIM-DWI and DCE-MRI parameters of the sacral and iliac bone marrow and the synovial area of the joint space in normal rats. The parameters in normal rats can be used in future research to compare to similar parameters in animal models or patients with SIJ diseases. This study serves as a guide for future research in SIJ diseases.

scholarly journals Sacroiliac joint pain following iliac-bone marrow aspiration and biopsy: a cohort study

2019 ◽  
Vol 9 (3) ◽  
pp. 251-258 ◽  
Author(s):  
Carlos J Roldan ◽  
Billy K Huh ◽  
Thomas Chai ◽  
Larry C Driver ◽  
Juhee Song ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cohort Study ◽  
Sacroiliac Joint ◽  
Joint Pain ◽  
Bone Marrow Aspiration ◽  
Iliac Bone ◽  
Sacroiliac Joint Pain

scholarly journals Therapeutic Targets in Childhood B-Acute Lymphoblastic Leukemia : What about HER2/Neu?

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 15-16
Author(s):  
Margaux Camuset ◽  
Alice Huault ◽  
Audrey Grain ◽  
Beatrice Clemenceau ◽  
Fanny Rialland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Lymphoblastic Leukemia ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Age Groups ◽  
Therapeutic Targets ◽  
Antigen Expression ◽  
Multiparameter Flow Cytometry ◽  
Significant Difference ◽  
Measurable Residual Disease

Relapsed B-lineage acute lymphoblastic leukemia (ALL) in children is associated with poor prognosis. Therefore, it seems essential to stratify patients according to prognostic factors in order to adapt therapies. The evaluation of minimal/measurable residual disease (MRD) during chemotherapy constitutes the most powerful prognostic factor in all age groups, indeed, patients with early low MRD having better outcomes. New immunotherapies based on monoclonal antibodies have recently been developed and are giving promising results in chemoresistant forms with limited toxicities. Indeed, CD20, CD38, CD22 and, less developed, HER2/neu constitute therapeutic targets. However, few studies have reported on the expression level of these markers and compared it to the normal counterpart, precursor B-cells or hematogones. Such investigations would be useful to appreciate the potential efficacy of immunotherapies targeting these markers. Moreover, it could help for the detection of minimal/measurable residual disease during follow-up. A cohort of 125 B-ALL patients (1-25 years old) was retrospectively enrolled in this study, treated between January 2011 and February 2020. Samples collected from bone marrow or peripheral blood were studied in multiparameter flow cytometry (MFC). Cells were analyzed with a Canto II flow cytometer (BD Biosciences), and Diva (BD Biosciences) software was used to assess the expression of the antigenic markers. The same MFC assay had been performed over the whole period, allowing for fluorescence intensities comparison. Additionally, results from 36 normal bone marrow samples were examined, to compare the level of antigen expression by hematogones. CD20, CD38 and CD22 were expressed in respectively 53.6%, 99.2% 98.4% of the cases, rather homogeneously and at intermediate levels. The mean fluorescence intensity of CD38 was much higher on hematogones than blasts, which made it a leukemia-associated immunophenotype (LAIP) for 101 patients (81.4%). HER2/neu, a marker of breast cancer also expressed in a subset of ALL, was present in 16 samples (13.4%), but not detectable on hematogones, and can thus always be considered a LAIP. Interestingly, in this subgroup, patients had a significantly lower 5-year EFS compared to patients without expression of HER2/neu (63% versus 80.5%, p=0.02) (Figure 1). No significant difference in the expression of tested potential therapeutic markers was found between age groups. In conclusion, these 4 antigens have sufficient expression intensities to make them potential therapeutic targets, which could be an interesting alternative for the treatment of refractory/relapsed childhood B-ALL. For example, trastuzumab could be a potential immunotherapy in the HER2/neu expressing group, especially regarding their poorer prognosis. Moreover, CD38 and HER2/neu are good candidates for monitoring MRD. These results are of interest as it has been shown that monitoring MRD early was prognostic on patients' outcome. Figure Disclosures Chevallier: Incyte Corporation: Honoraria.

Chromosomal alterations and DNA content in rats during ageing

Genome ◽  
1989 ◽  
Vol 32 (3) ◽  
pp. 389-392 ◽  
Author(s):  
Sarmistha Sen ◽  
Geeta Talukder ◽  
Archana Sharma
Keyword(s):  
Bone Marrow ◽  
Dna Content ◽  
Bone Marrow Cells ◽  
Age Groups ◽  
Chromosome Analysis ◽  
Male Rats ◽  
Age Related ◽  
Significant Difference ◽  
Feulgen Cytophotometry

Chromosome analysis of bone marrow cells from 96 sex-matched Rattus norvegicus of 12 different age groups showed a significant increase in hypodiploid cells with ageing. However, in meiotic preparations from gonadal cells of male rats the frequency of hypodiploid or hyperdiploid cells did not change significantly. In situ DNA estimation of bone marrow nuclei, following Feulgen cytophotometry, also did not show any significant difference with gradual age changes.Key words: senescence, DNA content, age-related changes.

scholarly journals High Kellgren-Lawrence Grade and Bone Marrow Lesions Predict Worsening Rates of Radiographic Joint Space Narrowing; The SEKOIA Study

2016 ◽  
Vol 43 (3) ◽  
pp. 657-665 ◽  
Author(s):  
Mark H. Edwards ◽  
Camille Parsons ◽  
Olivier Bruyère ◽  
Forence Petit Dop ◽  
Roland Chapurlat ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Plain Radiograph ◽  
Joint Space Width ◽  
Joint Space ◽  
Rate Of Change ◽  
Mri Findings ◽  
Men And Women ◽  
Tibiofemoral Joint ◽  
Knee Oa ◽  
Knee Mri

Objective.Determinants of radiographic progression in osteoarthritis (OA) are poorly understood. We investigated which features on baseline magnetic resonance imaging (MRI) acted as predictors of change in joint space width (JSW).Methods.A total of 559 men and women over the age of 50 years with clinical knee OA [Kellgren-Lawrence (KL) grade 2–3] were recruited to the placebo arm of the SEKOIA study (98 centers; 18 countries). Minimal tibiofemoral joint space and KL grade on plain radiograph of the knee were assessed at baseline and at yearly followup up to 3 years. In a subset, serial knee MRI examinations were performed. Individuals with a bone marrow lesion (BML) ≥ grade 2 at the tibiofemoral joint at baseline were classified as BML-positive. Relationships between change in JSW and risk factors were assessed using linear regression.Results.The mean age of study participants was 62.8 (SD 7.5) years and 73% were female; 38.6% had BML. Mean baseline JSW was 3.65 mm. This reduced by 0.18 (0.30) mm/year in men and 0.13 (0.23) mm/year in women. Those with BML had a significantly higher rate of annualized change in JSW; this relationship remained robust after adjustment for age, sex, and baseline KL grade [β = −0.10 (95% CI −0.18, −0.02) mm/yr]. Age, sex, baseline KL grade, and other MRI findings did not influence the rate of change in JSW.Conclusion.The rate of change in JSW was similar in men and women. BML on knee MRI predicted the rate of radiographic change in JSW. This relationship was independent of age, sex, and baseline KL grade.

scholarly journals A Histological Study of Vasoconstriction by Local Anesthetics in Mandible

2018 ◽  
Vol 65 (4) ◽  
pp. 244-248
Author(s):  
Katsunori Tanaka ◽  
Kanae Kudo ◽  
Kimiharu Ambe ◽  
Hiroyoshi Kawaai ◽  
Shinya Yamazaki
Keyword(s):  
Bone Marrow ◽  
Local Anesthetics ◽  
Histological Study ◽  
Statistical Processing ◽  
Lumen Area ◽  
Thin Sections ◽  
Periodontal Membrane ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Mandibular First Molar

To assess the effect of epinephrine-containing local anesthetics on vasoconstriction, we immunohistochemically measured the intravascular lumen area in different regions of the mandible. Twelve male Wistar rats were used. General anesthesia was induced and maintained with sevoflurane. Infiltration anesthesia was performed with 0.2 mL of epinephrine-free 2% lidocaine (E−) near the left mandibular first molar and with 0.2 mL of epinephrine-containing 2% lidocaine (E+) near the right mandibular first molar. After decalcification, the specimens were paraffinized, and thin sections were prepared and immunohistologically stained with an antismooth muscle actin antibody. The intravascular lumen area was measured in the mucosa, periodontal membrane, Haversian/Volkmann's canal, and bone marrow. A Mann-Whitney U test was used for statistical processing, and p < .05 was considered to indicate a statistically significant difference. In the oral mucosa and the periodontal membrane, E+ had a significantly smaller vascular lumen area than E−. In the Haversian/Volkmann's canal and the bone marrow, no significant intergroup difference was observed in the intravascular lumen area. We postulate that this is due to a low smooth muscle content of blood vessels in the mandible and suggest that the vasoconstrictive effect of epinephrine-containing local anesthetics within the mandible is ineffective.

scholarly journals Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells

2019 ◽  
Vol 13 (02) ◽  
pp. 238-242
Author(s):  
Sri Wigati Mardi Mulyani ◽  
Eha Renwi Astuti ◽  
Otty Ratna Wahyuni ◽  
Diah Savitri Ernawati ◽  
Nastiti Faradilla Ramadhani
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Statistical Analysis ◽  
Salivary Glands ◽  
Low Oxygen ◽  
Hypoxic Precondition ◽  
Treatment Groups ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract Objectives The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O2 concentration. Materials and Methods Stem cell culture was performed under normoxic (O2: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N2, 5% CO2, and 1% O2. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation. Statistical Analysis Statistical analysis was done using normality test, followed by MANOVA test (p < 0.05). Results There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (p < 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia. Conclusion BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.

scholarly journals Comparison of three methods for calculation of sacroiliac joint index in different age groups in bone scintigraphy

2020 ◽  
Author(s):  
Ebru Salmanoglu
Keyword(s):  
Bone Scintigraphy ◽  
Sacroiliac Joint ◽  
Age Groups ◽  
Region Of Interest ◽  
Threshold Value ◽  
Mean Value ◽  
Data Set ◽  
Mean Values ◽  
Significant Difference ◽  
Group 2

Abstract BackgroundIt was aimed to compare three methods for calculation of sacroiliac joint (SIJ) index in different age groups. Cases undergone Tc-99m MDP bone scintigraphy and images were evaluated. Cases don’t have pathologic uptake were performed posterior pelvic images for calculation of SIJ index.Normal cases (n:160) were included.Cases were separated into four age groups, group 1(3-20 years), group 2(21-40 years), group 3(41-60 years), group 4(61 years and older). For the first method,irregular region of interest (ROI) was selected,for the second method rectangular ROI was selected.For the third method, profile peak counts were used. Count of each SIJ was divided count of sacrum to calculate right, left and mean SIJ indices.ResultsThere was no statistically significant difference between mean values of right and left SIJ index for three methods(p>0.05).Means of female were lower than male for right, left and and mean SIJ indices.Lowest mean values of right SIJ index, left SIJ index and mean SIJ index were found in ≥61 age group in both genders.All of three methods were significantly different from each other according to four age groups(p<0.05). First method had quite lower mean value than second and third methods for right SIJ index.A negative statistically significant correlation was found between age and SIJ index for all of three methods(p<0.01). Statistically significant difference was found between three methods for each age gropus based on mean SIJ index(p<0.05).ConclusionsEach nuclear medicine center should establish their own threshold value according to their own data set in normal cases and patients.

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