scholarly journals Characterization of Ethanol Producing Yeasts for Their Efficiency in Ethanol production, Salt Tolerance, and Utilization of Glucose and Xylose.

2019 ◽  
Author(s):  
Jarina Joshi ◽  
Tribikram Bhattarai ◽  
Amar Yadav ◽  
Lakshmaiah Sreer
Keyword(s):  
Salt Tolerance ◽  
Ethanol Production ◽  
Yeast Strain ◽  
Production Efficiency ◽  
Rrna Gene ◽  
Mrna Levels ◽  
Salt Tolerant ◽  
Alcohol Production ◽  
Yeast Strains

Abstract Yeast are the mainstay in ethanol production industry. Search for efficient yeast strains that are salt tolerant and utilize both hexoses (glucose) and pentoses (xylose and arabinose) is important in fermentation industry. In this regard,12 yeast strains, viz., CDBT1-12, were isolated from various sources and characterized. Molecular characterization of the yeast strains was done by sequencing 26S rRNA gene, D1D2 region. Out of 12 isolates, 10 were found to be Saccharomyces cerevisiae , CDBT7 was Wikerhamomyces anomalous , and CDBT8 was Cyberlindnera fabianii . The yeast isolates were characterized in terms of their ethanol production efficiency, salt tolerance and ability to utilize of glucose and xylose. All the strains were found to be good ethanol producers. Yeast strain CDBT2 was found to have tolerance for high salt (up to 15%) and ethanol (up to 16%) concentrations. Yeast strain CDBT7 was found to utilize both glucose and xylose without compromising on ethanol production efficiency. The CDBT7 strain was also salt tolerant (up to15%).Yeast strain CDBT2 when grown in an electrochemical cell with low levels of applied external voltage, alcohol dehydrogenase (ADH1) and pyruvate decarboxylase (PDC1) mRNA levels were increased by 2.78 ± 0.80 and 1.12 ± 0.37 fold, respectively. We believe, the latter observation is novel and it has not been reported previously. It also further supports our previous observation of increase in level of alcohol production by CDBT2 strain in the presence of applied electrical current.

scholarly journals Characterization of Ethanol Producing Yeasts for Their Efficiency in Ethanol production, Salt Tolerance, and Utilization of Glucose and Xylose

2020 ◽  
Author(s):  
Jarina Joshi ◽  
Tribikram Bhattarai ◽  
Amar Yadav ◽  
Lakshmaiah Sreer
Keyword(s):  
Salt Tolerance ◽  
Ethanol Production ◽  
Yeast Strain ◽  
Production Efficiency ◽  
Rrna Gene ◽  
Mrna Levels ◽  
Salt Tolerant ◽  
Alcohol Production ◽  
Yeast Strains

Abstract Yeasts are the mainstay in ethanol production industry. Search for efficient yeast strains that are salt tolerant and utilize both hexoses (glucose) and pentoses (xylose and arabinose) is important in fermentation industry. In this regard,12 yeast strains, viz., CDBT1-12, were isolated from various sources and characterized. Molecular characterization of the yeast strains was done by sequencing 26S rRNA gene, D1D2 region. Out of 12 isolates, 10 were found to be Saccharomyces cerevisiae , CDBT7 was Wikerhamomyces anomalous , and CDBT8 was Cyberlindnera fabianii . The yeast isolates were characterized in terms of their ethanol production efficiency, salt tolerance and ability to utilize of glucose and xylose. All the strains were found to be good ethanol producers. Yeast strain CDBT2 was found to have tolerance for high salt (up to 15%) and ethanol (up to 16%) concentrations. Yeast strain CDBT7 was found to utilize both glucose and xylose without compromising on ethanol production efficiency. The CDBT7 strain was also salt tolerant (up to15%).Yeast strain CDBT2 when grown in an electrochemical cell with low levels of applied external voltage, alcohol dehydrogenase (ADH1) and pyruvate decarboxylase (PDC1) mRNA levels were increased by 2.78 ± 0.80 and 1.12 ± 0.37 fold, respectively. We believe, the latter observation is novel and it has not been reported previously. It also further supports our previous observation of increase in level of alcohol production by CDBT2 strain in the presence of applied electrical current.

scholarly journals Identification and Characterization of a Novel Thermostable and Salt-Tolerant β-1,3 Xylanase from Flammeovirga pacifica Strain WPAGA1

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1287
Author(s):  
Zhiwei Yi ◽  
Zhengwen Cai ◽  
Bo Zeng ◽  
Runying Zeng ◽  
Guangya Zhang
Keyword(s):  
Thermal Stability ◽  
Salt Tolerance ◽  
3D Structure ◽  
Catalytic Efficiency ◽  
Dynamics Simulation ◽  
Carbohydrate Binding ◽  
Salt Tolerant ◽  
Excellent Thermal Stability ◽  
Moderately Thermophilic

β-1,3 xylanase is an important enzyme in the biorefinery process for some algae. The discovery and characterization of new β-1,3 xylanase is a hot research topic. In this paper, a novel β-1,3 xylanase (Xyl88) is revealed from the annotated genome of Flammeovirga pacifica strain WPAGA1. Bioinformatic analysis shows that Xyl88 belongs to the glycoside hydrolase 26 (GH26) with a suspected CBM (carbohydrate-binding module) sequence. The activity of rXyl88 is 75% of the highest enzyme activity (1.5 mol/L NaCl) in 3 mol/L NaCl buffer, which suggests good salt tolerance of rXy188. The optimum reaction temperature in the buffer without NaCl and with 1.5 mol/L NaCl is 45 °C and 55 °C, respectively. Notably, the catalytic efficiency of rXyl88 (kcat/Km) is approximately 20 higher than that of the thermophilic β-1,3 xylanase that has the highest catalytic efficiency. Xyl88 in this study becomes the most efficient enzyme ever found, and it is also the first reported moderately thermophilic and salt-tolerant β-1,3 xylanase. Results of molecular dynamics simulation further prove the excellent thermal stability of Xyl88. Moreover, according to the predicted 3D structure of the Xyl88, the surface of the enzyme is distributed with more negative charges, which is related to its salt tolerance, and significantly more hydrogen bonds and Van der Waals force between the intramolecular residues, which is related to its thermal stability.

scholarly journals Dynamics of microbial contaminants is driven by selection during ethanol production

2018 ◽  
Author(s):  
Luciano Lopes Queiroz ◽  
Maria Silveira Costa ◽  
Alcilene de Abreu Pereira ◽  
Marcelo de Paula Avila ◽  
Patrícia Silva Costa ◽  
...  
Keyword(s):  
Species Richness ◽  
Production Process ◽  
Ethanol Production ◽  
Production Efficiency ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Increase Production Efficiency ◽  
Community Assemblage ◽  
Sequential Samples ◽  
Rrna Gene Sequencing

AbstractBrazil is the second largest ethanol producer in the World and largest using sugarcane feedstock. Bacteria contamination is one the most important issues faced by ethanol producers that seek to increase production efficiency. Each step of production is a selection event due to the environmental and biological changes that occur. Therefore, we evaluated the influence of the selection arising from the ethanol production process on diversity and composition of Bacteria. Our objectives were to test two hypothesis, (1) that species richness will decrease during the production process and (2) that Lactic Acid Bacteria will become dominant with the advance of ethanol production due to selection. Bacterial community assemblage was accessed using 16S rRNA gene sequencing from 19 sequential samples. Temperature is of great importance in shaping microbial communities. Species richness increased between the Decanter and Must steps of the process. Low Simpson index values were recorded at the fermentation step, indicating a high dominance ofLactobacillus. Interactions betweenLactobacillusand yeast may be impairing the efficiency of industrial ethanol production.

scholarly journals A Mutated Yeast Strain with Enhanced Ethanol Production Efficiency and Stress Tolerance

2012 ◽  
Vol 2 (2) ◽  
pp. 100-115 ◽  
Author(s):  
Naghmeh Hemmati ◽  
David A. Lightfoot ◽  
Ahmed Fakhoury
Keyword(s):  
Ethanol Production ◽  
Stress Tolerance ◽  
Yeast Strain ◽  
Production Efficiency

scholarly journals Application of yeast with reduced alcohol yield for sparkling wine production

2019 ◽  
Vol 12 ◽  
pp. 02021 ◽  
Author(s):  
M. Schmitt ◽  
S. Broschart ◽  
C.-D. Patz ◽  
D. Rauhut ◽  
M. Friedel ◽  
...  
Keyword(s):  
Yeast Strain ◽  
Wine Industry ◽  
Sparkling Wine ◽  
Alcohol Content ◽  
Wine Production ◽  
Alcohol Production ◽  
Volatile Acidity ◽  
Yeast Strains ◽  
Lower Alcohol ◽  
Commercial Yeast

Two commercial yeast strains with reduced alcohol production in comparison with a commercial yeast strain with common alcohol yield were assed for their suitability in sparkling wine production according to the traditional bottle fermentation. The different yeast strains were applied for the first fermentation. As expected the base wine differed in terms alcohol. Furthermore the yeast with lower alcohol content showed higher values of glycerol, higher arginine content and in the same time reduced levels of proline after fermentation. However those samples showed increased volatile acidity values, compared to the control wines. The later bottle fermentation with a uniform yeast strain showed similar fermentation kinetics for all four lots. Sensory evaluation showed no clear differences between the sparkling wines that were stored 9 months on the lees. The base wines nevertheless clearly differed from each other. Besides the increased production of volatile acidity, the tested yeast strains with lower alcohol production appear very promising for the sparkling wine industry to face the generally rising alcohol contents worldwide.

scholarly journals Comparative Proteomics of Salt-Tolerant and Salt-Sensitive Maize Inbred Lines to Reveal the Molecular Mechanism of Salt Tolerance

2019 ◽  
Vol 20 (19) ◽  
pp. 4725
Author(s):  
Fenqi Chen ◽  
Peng Fang ◽  
Yunling Peng ◽  
Wenjing Zeng ◽  
Xiaoqiang Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Molecular Mechanism ◽  
Inbred Lines ◽  
Comparative Proteomics ◽  
Venn Diagram ◽  
Mrna Levels ◽  
Salt Tolerant ◽  
Great Loss ◽  
Maize Inbred Lines

Salt stress is one of the key abiotic stresses that causes great loss of yield and serious decrease in quality in maize (Zea mays L.). Therefore, it is very important to reveal the molecular mechanism of salt tolerance in maize. To acknowledge the molecular mechanisms underlying maize salt tolerance, two maize inbred lines, including salt-tolerant 8723 and salt-sensitive P138, were used in this study. Comparative proteomics of seedling roots from two maize inbred lines under 180 mM salt stress for 10 days were performed by the isobaric tags for relative and absolute quantitation (iTRAQ) approach. A total of 1056 differentially expressed proteins (DEPs) were identified. In total, 626 DEPs were identified in line 8723 under salt stress, among them, 378 up-regulated and 248 down-regulated. There were 473 DEPs identified in P138, of which 212 were up-regulated and 261 were down-regulated. Venn diagram analysis showed that 17 DEPs were up-regulated and 12 DEPs were down-regulated in the two inbred lines. In addition, 8 DEPs were up-regulated in line 8723 but down-regulated in P138, 6 DEPs were down-regulated in line 8723 but up-regulated in P138. In salt-stressed 8723, the DEPs were primarily associated with phenylpropanoid biosynthesis, starch and sucrose metabolism, and the mitogen-activated protein kinase (MAPK) signaling pathway. Intriguingly, the DEPs were only associated with the nitrogen metabolism pathway in P138. Compared to P138, the root response to salt stress in 8723 could maintain stronger water retention capacity, osmotic regulation ability, synergistic effects of antioxidant enzymes, energy supply capacity, signal transduction, ammonia detoxification ability, lipid metabolism, and nucleic acid synthesis. Based on the proteome sequencing information, changes of 8 DEPs abundance were related to the corresponding mRNA levels by quantitative real-time PCR (qRT-PCR). Our results from this study may elucidate some details of salt tolerance mechanisms and salt tolerance breeding of maize.

The Characterization of Salt Tolerance in Biomining Microorganisms and the Search for Novel Salt Tolerant Strains

2009 ◽  
Vol 71-73 ◽  
pp. 283-286 ◽  
Author(s):  
Carla M. Zammit ◽  
L.A. Mutch ◽  
Helen R. Watling ◽  
Elizabeth L.J. Watkin
Keyword(s):  
Salt Tolerance ◽  
Growth Rates ◽  
Salt Tolerant ◽  
Acidic Media ◽  
Comparative Tests ◽  
Nacl Tolerance ◽  
Oxidation Rates ◽  
Australian Wheat ◽  
Western Australian

In this study an acidic saline drain in the Western Australian wheat belt was sampled and enriched for salt tolerant chemolithotrophic microorganisms in acidic media containing up to 100 gL-1 NaCl. A mixed consortium was obtained which grows at pH 1.8 and oxidises iron (II) in the presence of up to 30 gL-1 NaCl. In comparative tests (growth rates and iron (II) oxidation rates) it was found that NaCl concentrations >3.5 gL-1 generally cause reduced growth and iron (II) oxidation rates in known biomining organisms. The results help to set a benchmark for NaCl tolerance in known biomining microorganisms and will lead to the generation of a consortium of microorganisms that can oxidise iron (II) effectively in saline process water.

scholarly journals Formation and characterization of biofilms formed by salt-tolerant yeast strains in seawater-based growth medium

2021 ◽  
Vol 105 (6) ◽  
pp. 2411-2426
Author(s):  
Robert Zarnowski ◽  
Hiram Sanchez ◽  
Cecilia Andreu ◽  
David Andes ◽  
Marcel·lí del Olmo
Keyword(s):  
Growth Medium ◽  
Salt Tolerant ◽  
Yeast Strains
Sign in / Sign up

Export Citation Format

Share Document