Effect of Resistance and Endurance Training on Gene Expression of Adiponectin and its Receptors (AdipoR1 and AdipoR2) in Testicles and Serum Levels of Sex Hormones in Diabetic Rats

2020 ◽  
Author(s):  
Zahra Nadi ◽  
Mohammad Bayat ◽  
hadi karami ◽  
Mohammad Parastesh ◽  
parvindokht bayat
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Endurance Training ◽  
Sex Hormones ◽  
Fasting Blood Glucose ◽  
Serum Testosterone ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Adiponectin Gene ◽  
Testosterone Levels

Abstract Background Sex hormone, adiponectin and its receptors interacted in the testes. Diabetes can also interfere with this interaction. Regular exercise has some effects on the diabetes side effects. This study purpose was to investigate the effects resistance and endurance training on adiponectin gene expression and its receptors and sex hormones in those rats with diabetes.MethodsIn this experimental study, 48 male Wistar rats were divided into 6 groups by random. After performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone.Results The induction of diabetes mellitus STZ-NA significantly increased the serum level of fasting blood glucose, the gene expression of adiponectin and also the AdipoR 1 in the testicles of diabetic rats in comparison with healthy controls. In addition, diabetes resulted in a significant reduction in serum testosterone levels and LH in diabetic group, but it has no effect on FSH.Conclusions Resistance and endurance training decreased the blood glucose with a significant amount reduction in adiponectin levels and AdipoR 1 gene rats, and also increased the serum testosterone levels and LH in diabetic rats. Resistance and endurance training can improve expression of adiponectin and AdipoR 1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.

scholarly journals Effect of Exercise Training on Gene Expression of Adiponectin and its Receptors in Testicles and Sex Hormones in Diabetic Rats

2021 ◽  
Author(s):  
Zahra Nadi ◽  
Mohammad Bayat ◽  
Hadi Karami ◽  
Mohamad Parastesh ◽  
ParvinDokht Bayat
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Sex Hormones ◽  
Fasting Blood Glucose ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Male Sex ◽  
Group Resistance ◽  
Type 2 Diabetic

Adiponectin and its receptors are expressed in the male reproductive system, which play a role in regulating male sex hormones and fertility. Diabetes was induced by Streptozotocin-Nicotinamide (STZ-NA i.p) in rats and after performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone. The STZ-NA significantly increased the fasting blood glucose, gene expression of adiponectin AdipoR1 in the testicles of diabetic rats. A significant reduction in serum testosterone and LH levels were observed in the diabetic group. Resistance and endurance training decreased blood glucose, adiponectin and AdipoR1 gene, and also increased the serum testosterone and LH levels in diabetic rats. Overall, our data suggest the role played by training in improve expression of adiponectin and AdipoR1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.    

scholarly journals Effect of Exercise Training on Gene Expression of Adiponectin and its Receptors in Testicles and Sex Hormones in Diabetic Rats

2021 ◽  
Author(s):  
Zahra Nadi ◽  
Mohammad Bayat ◽  
Hadi Karami ◽  
Mohamad Parastesh ◽  
ParvinDokht Bayat
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Sex Hormones ◽  
Fasting Blood Glucose ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Male Sex ◽  
Group Resistance ◽  
Type 2 Diabetic

Adiponectin and its receptors are expressed in the male reproductive system, which play a role in regulating male sex hormones and fertility. Diabetes was induced by Streptozotocin-Nicotinamide (STZ-NA i.p) in rats and after performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone. The STZ-NA significantly increased the fasting blood glucose, gene expression of adiponectin AdipoR1 in the testicles of diabetic rats. A significant reduction in serum testosterone and LH levels were observed in the diabetic group. Resistance and endurance training decreased blood glucose, adiponectin and AdipoR1 gene, and also increased the serum testosterone and LH levels in diabetic rats. Overall, our data suggest the role played by training in improve expression of adiponectin and AdipoR1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.    

scholarly journals Polygonatum sibiricum extract exerts inhibitory effect on diabetes in a rat model

2021 ◽  
Vol 18 (7) ◽  
pp. 1493-1497
Author(s):  
Lai-zeng Yu ◽  
Xue-peng Zhang ◽  
Ying-xin Wang
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Dependent Manner

Purpose: To investigate the effect of Polygonatum sibiricum extract (PSE) on streptozotocin-induced diabetic rats. Methods: PSE was obtained by steeping the dried Polygonatum sibiricum in water at 60 oC three times, each for 1 h, before first drying in an oven at 100C and then freeze-drying the final extract, thus obtained. Diabetic model rats were prepared by a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control, normal control, reference (glibenclamide1 mg/kg) as well as PSE groups, (35, 70 and 140 mg/kg). Blood glucose and plasma insulin levels were measured to determine antihyperglycemic effect. Oxidative stress was evaluated in liver and kidney by their antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats. Results: Compared with diabetic rats, oral administration of PSE at a concentration of 120 mg/kg daily for 30 days showed a significant decrease in fasting blood glucose (118.34 ± 3.29 mg/dL) (p < 0.05) and increased insulin level (12.86± 0.62 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.83 ±0.21 mg/dL, p < 0.05) and serum urea (43.26±1.42 mg/dL, p < 0.05). Conclusion: The results suggest that PSE may effectively normalize impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. Thus, PSE has a protective effect against lipid peroxidation by scavenging free radicals, restoration of insulin function, and reduction of the incidence of complications.

A comparative study of the antidiabetic effect of two training protocols in streptozotocin-nicotinamide diabetic rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohammad Bayat ◽  
Mona Alaee ◽  
Ahmad Akbari ◽  
Mehdi Sadegh ◽  
Seied Amirhossein Latifi ◽  
...  
Keyword(s):  
Resistance Training ◽  
Endurance Training ◽  
Fasting Blood Glucose ◽  
Training Group ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Lipid Profiles ◽  
Control Group ◽  
Metabolic Derangement ◽  
Better Regulation

AbstractBackgroundPhysical inactivity is the major risk factor for type 2 diabetes (T2D). The present study was conducted to investigate the effects of resistance training and endurance training on diabetic-related metabolic parameters in diabetic rats.Materials and methodsTwenty-four male Wistar rats were randomly assigned to four groups of six rats each: control group (C), diabetic group (D), resistance training group (RES) and endurance training group (END). T2D was induced intraperitoneally using nicotinamide (120 mg/kg) and streptozotocin (STZ, 65 mg/kg). The training period was 70 days. The irisin, betatrophin, insulin, fasting blood glucose (FBG) and lipid profiles were measured in the serum of all rats.ResultsDiabetes significantly increased serum levels of FBG (p < 0.001), which were decreased significantly after the administration of training (p < 0.001). Training administration had a significant effect in normalizing serum lipid profiles (p < 0.001) and it was shown to increase the serum levels of irisin, betatrophin (p < 0.001) and insulin (END: p < 0.001 and resistance training: p < 0.05). It was also found that the endurance training was more effective in improving this parameters when compared with resistance training (p < 0.05). In addition, the irisin revealed a significant positive association with betatrophin (END: p < 0.01 and resistance training: p < 0.05) and insulin (END: p < 0.01 and RES: p < 0.05) values in diabetic groups.ConclusionThis study demonstrated that endurance training was more effective in diabetic related metabolic derangement compared with resistance training. This effect is probably due to better regulation of irisin, betatrophin and insulin relative to resistance training.

scholarly journals Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats

2020 ◽  
Vol 71 (11) ◽  
pp. 101-110
Author(s):  
Tayyeba Ramzan ◽  
Bilal Aslam ◽  
Faqir Muhammad ◽  
Muhammad Naeem Faisal ◽  
Asif Hussain
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Liver Tissue ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Ocimum Sanctum ◽  
Protective Effects ◽  
Oxidative Stress Biomarkers ◽  
Glucose Levels

Ocimum sanctum L. is widely used as traditional remedy to manage hyperglycemia. This study was aimed to evaluate the effect of aqueous-methanolic extract of O. sanctum leaves (OSE) on the anti-diabetic activity of gliclazide in alloxan-induced diabetes in Wistar rats. Diabetes was induced by intraperitoneally injecting alloxan (120 mg/kg b.w.) in rats. Treatments including OSE (100 mg/kg b.w.), gliclazide (100 mg/kg b.w.), and in combination were given daily to diabetic rats till the 21st day of study. Body weight and fasting blood glucose levels were determined at regular intervals, while blood and organ samples were taken at the end of the study for biochemical and histopathological studies. Results showed that treatments exhibited anti-hyperglycemic activity through significantly (p [ 0.05) restoring body weight, fasting blood glucose level, and serum levels of glucose, insulin and HbA1c. The anti-lipidemic activity was noticed as total cholesterol (TC), triglyceride (TG), high-/low density-lipoproteins (HDL-C, LDL-C) levels were restored in treated diabetic rats. Ameliorative effects of treatments were observed as significant (p [ 0.05) reduction in serum levels of liver function biomarkers (alanine aminotransferase; ALT, aspartate aminotransferase; AST, alkaline phosphatase; ALP and bilirubin; BIL) and restoration of oxidative stress biomarkers (catalase; CAT, superoxide dismutase; SOD and malondialdehyde; MDA) in liver tissue. Histopathological findings supported these results as an increase in pancreatic islets size and protective effects on liver tissue was observed in diabetic rats treated with gliclazide and OSE alone and their combination. Conclusively, the combination of OSE and gliclazide produced a synergistic anti-diabetic effect as compared to that of alone treatment.

scholarly journals Antidiabetic and Antinephropathic Potential of Ajwa Pit & Pulp (Phoenix dactylifera) in Alloxanized Diabetic Rats

2020 ◽  
Vol 34 (1) ◽  
pp. 39-43
Author(s):  
Imran Maqsood Butt
Keyword(s):  
Blood Glucose ◽  
Creatinine Clearance ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Phoenix Dactylifera ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Medical Institute ◽  
Urine Creatinine

Introduction: Generally in the world, diabetes is the most significant cause of deaths and morbidity. Blood glucose lowering agents possess remarkable adverse effects like weight gain and hypoglycemia and thus hindering the achievement of glycemic control. Since earliest times till today, herbal preparations have been utilized for management of diabetes and its complications. Aims & Objectives: To investigate antihyperglycemic and nephroprotective effects of Ajwa pit and pulp in alloxanized - diabetic and nephropathic rats. Place and duration of study: This study was conducted at Postgraduate Medical Institute, Lahore for the duration of four weeks. Material & Methods: Adult male rats (weighing 120-150 g), total of 32 were purchased and divided into 4 groups. The group1 was normal control whereas group2 was diabetic control. Diabetes was induced in groups 2, 3 and 4 with 150mg/kg single alloxan monohydrate1.P Injection. Groups 3 and 4 were given Ajwa pit and pulp rations for 4 weeks. Blood and urine samples were taken on zero and 4 weeks for fasting blood glucose, serum and urine biochemical variables for renal functions. Results: The results revealed that Ajwa pits significantly decreased serum levels of glucose (252±60 vs. 348±67 mg/dl), serum urea (38±7.0 vs. 63±9.6 mg/dl), serum creatinine (0.39±0.1 vs. 0.58±0.1 mg/dl) and microalbuminuria marker (7.8±1.7 vs. 9.0±2.2mg/l). Moreover it improved urine creatinine (14.0±2.9 vs.10.0±2.7 mg/dl) and creatinine clearance (0.226±0.08 vs 0.117±0.04 ml/min) when compared to diabetic control rats. Effect of Ajwa pulp was far less than Ajwa pit. Ajwa pulp did not significantly decrease serum levels of glucose (290±60 vs. 348±67 mg/dl), urea (51±9.5 vs. 63±9.6 mg/dl), creatinine (0.66±0.2 vs. 0.58±0.1 mg/dl) and microalbuminuria marker (8.3±2.0 vs. 9.0±2.2mg/l). Also there was no improvement in urine creatinine (11.1±1.6 vs. 10.0±2.7 mg/dl) and creatinine clearance (0.130±0.05 vs 0.117±0.04 ml/min) levels when compared to diabetic control rats. Conclusion: Ajwa pit possesses strong antihyperglycemic and protective effect in renal damage by prolonged diabetes mellitus. Highest polyphenols, bioflavonoids and antioxidants are presumed to be responsible for this effect.

Metformin, sitagliptin, and liraglutide modulate serum retinol-binding protein-4 level and adipocytokine production in type 2 diabetes mellitus rat model

2018 ◽  
Vol 96 (12) ◽  
pp. 1226-1231 ◽  
Author(s):  
Noha A.T. Abbas ◽  
Amal El. Salem
Keyword(s):  
Insulin Resistance ◽  
Blood Glucose ◽  
Body Mass ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Treated Group ◽  
Diabetic Rats ◽  
Retinol Binding Protein ◽  
Serum Retinol ◽  
Daily Group

Many adipocytokines correlate with obesity and insulin resistance. We examined the effects of metformin, sitagliptin, and liraglutide in diabetic rats. Group 1: control normal (CN) rats received oral saline daily. Group 2: diabetic non-treated (DNT) rats were injected with streptozotocin (STZ) to get diabetic then after 72 h received oral saline daily. Group 3: rats were injected with STZ then after 72 h were treated with metformin (200 mg/kg) orally. Group 4: rats were injected with STZ then after 72 h received sitagliptin 6 mg/kg orally twice daily. Group 5: rats were injected with STZ then after 72 h were treated with liraglutide at a dose of 0.3 mg/kg every 12 h subcutaneous injection. After 8 weeks, body mass, fasting blood glucose, adipocytokines, and lipid profile were assessed. From the results, we concluded that the 3 drugs improved blood glucose and insulin resistance with correction of adipocytokines serum levels; however, the liraglutide-treated group was the only group that showed significant body mass reduction.

scholarly journals Antihyperglycemic and Antioxidant Activity of Fructus hordei Germinatus Extract on Streptozotocin-induced Diabetic Rats

2015 ◽  
Vol 14 (9) ◽  
pp. 1651-1657
Author(s):  
H Wei ◽  
X Liang ◽  
B Wu ◽  
J Zhang ◽  
Y Qin ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Dependent Manner ◽  
Liver And Kidney ◽  
Plasma Insulin Levels ◽  
Antioxidant Markers

Purpose: To explore the antihyperglycemic and antioxidant effects of Fructus Hordei Germinatus extract (FHGE) in diabetic rats.Methods: The aqueous extract of FHG was obtained by steeping the dried Fructus Hordei Germinatus in water at 60oC three times, each for 1 h before first drying in an oven and then freeze-drying the last extract thus obtained. Antihyperglycemic effect was measured by blood glucose and plasma insulin levels. Oxidative stress was evaluated in liver and kidney by antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT), while blood serum levels of creatinine and urea were also determined in both diabetic control and treated rats.Results: Compared with diabetic rats, oral administration of FHGE at a concentration of 20, 40 and 80 mg/kg daily for 45 days showed a significant decrease in fasting blood glucose (p < 0.05) and increased insulin (p < 0.05) level,. Furthermore, it significantly reduced biochemical parameters (serum creatinine and serum urea, p < 0.05)). The treatment also resulted in significant increase in GSH, GPx (p < 0.05), SOD and CAT (p < 0.05), as well as decreased LPO (p < 0.05) level in the liver and kidney of diabetic rats.Conclusion: The results suggest that FHGE may effectively normalize the impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. FHGE has a protective effect against lipid peroxidation by scavenging free radicals and is thus capable of reducing the risk of diabetic complications.Keywords: Fructus hordei Germinatus, Diabetic, Antihyperglycemic, Lipid peroxidation, Antioxidant, Fasting blood glucose, Creatinine, Urea

scholarly journals Tribulus terrestris Efficacy and Safety Concerns in Diabetes and Erectile Dysfunction, Assessed in an Experimental Model

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 744
Author(s):  
Ruxandra Ștefănescu ◽  
Lenard Farczadi ◽  
Adina Huțanu ◽  
Bianca E. Ősz ◽  
Marius Mărușteri ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Diabetes Complications ◽  
Fasting Blood Glucose ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Testosterone Levels ◽  
Diabetic Control Group ◽  
Elevated Blood Glucose Level

The present project aims to evaluate Tribulus terrestris (TT) extracts by addressing various possible mechanisms of action in order to see whether the use of TT supplements in diabetes and diabetes complications is justified. Diabetic rats were divided into three groups: diabetic control group, TT extract with low protodioscin content group (TT-LPC) and TT extract with high protodioscin content group (TT-HPC). After twelve weeks of treatment, fasting blood glucose, insulin, LH, FSH and testosterone levels were measured. Both TT preparations reduced elevated blood glucose level. Insulin and luteinizing hormone levels were not significantly different compared with the control group; however, the FSH and testosterone levels were significantly higher in the TT-HPC group compared with the diabetic control group. The testosterone level is correlated in part with the protodioscin concentration in extracts and is probably mediated through an FSH-linked pathway.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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