Klotho, fibroblast growth factors 19 and 21 serum concentrations in children and adolescents with normal body weight and obesity – and their associations with metabolic parameters.

Abstract Background: Fibroblast growth factor 19 (FGF19), Fibroblast growth factor 21 (FGF21) and Klotho are regulators of energy homeostasis. However, in the paediatric population the relationship between obesity, metabolic disorders and mentioned factors has not been clearly investigated. We analysed serum concentrations of FGF19, FGF21 and Klotho in children and adolescents with normal body weight as well as in overweight and obese subjects – and their associations with components of metabolic syndrome and insulin resistance (IR).Methods: The cross-sectional study conducted in the group of hospitalised children and adolescents. Laboratory investigation included serum ELISA tests for FGF19, FGF21, Klotho as well as lipid profile and oral glucose tolerance test for calculation of the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The clinical analysis included blood pressure measurement, body fat percentage estimation and assessing the prevalence of metabolic syndrome (MS) and its components. Results: The study was conducted on 174 children/adolescents aged 6-17 years divided into the following groups: with normal body weight (N=48), with obesity (N=92) and overweight subjects (N=34). Klotho levels were significantly higher in the group of subjects with obesity [median 168.6 pg/ml]) than those with overweight [131.3 pg/ml] and normal body weight [116.6 pg/ml] (p=0.0334). Median serum FGF21 level was elevated in the group of patients with MS in comparison to other subjects [136.2 pg/ml vs 82.6 pg/ml, p=0.0285]. Increased Klotho concentrations were noted in patients affected by IR compared with subjects with normal insulin sensitivity [185.3 pg/ml vs 132.6 pg/ml, p=0.0282]. Multivariable model for HOMA-IR showed FGF19 as an independent predictor for IR after adjusting for the pubertal stage and BMI Z-score.Conclusions: Klotho levels were associated with body weight status in children and adolescents. Moreover, Klotho, FGF19 and FGF21 concentrations correlated with IR status and traits of MS.

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Klotho and fibroblast growth factors 19 and 21 serum concentrations in children and adolescents with normal body weight and obesity and their associations with metabolic parameters

BMC Pediatrics ◽

10.1186/s12887-020-02199-2 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Anna Socha-Banasiak ◽

Arkadiusz Michalak ◽

Krzysztof Pacześ ◽

Zuzanna Gaj ◽

Wojciech Fendler ◽

...

Keyword(s):

Body Weight ◽

Growth Factors ◽

Children And Adolescents ◽

Fibroblast Growth Factors ◽

Metabolic Parameters ◽

Fibroblast Growth ◽

Serum Concentrations ◽

Normal Body ◽

Normal Body Weight

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Red raspberry (poly)phenolic extract improves diet-induced obesity, hepatic steatosis and insulin resistance in obese mice

Journal of Berry Research ◽

10.3233/jbr-200696 ◽

2021 ◽

pp. 1-14

Author(s):

Haizhao Song ◽

Xinchun Shen ◽

Qiang Chu ◽

Xiaodong Zheng

Keyword(s):

Insulin Resistance ◽

Body Weight ◽

Lipid Metabolism ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Fibroblast Growth Factor 21 ◽

Fibroblast Growth ◽

Red Raspberry ◽

Diet Induced Obesity ◽

Phenolic Extract

BACKGROUND: Red raspberry (Rubus idaeus L.), a natural dietary source of (poly)phenols, has been used as medicine for centuries. OBJECTIVE: The purpose of this study is to determine the effect of a red raspberry (poly)phenolic extract (RPE) on diet-induced obesity, hepatic steatosis and insulin resistance, and elucidate the underlying molecular mechanisms. METHODS: Male specific pathogen-free C57BL/6J mice were randomly divided into three groups (n = 12 per group), and fed with low-fat diet (10% fat energy), high-fat diet (HFD, 45% fat energy), or HFD supplemented with RPE of 150 mg/kg body weight by intragastric administration for 14 weeks. Obesity-related biochemical indexes and hepatic gene expression levels were determined. The statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test. RESULTS: The body weight gain, steatosis grade scores and insulin resistance index in the RPE group decreased by 34.48% (P = 0.00), 58.82% (P = 0.00), and 53.77% (P = 0.00), respectively, compared to those in the HFD group. Moreover, RPE supplement significantly changed the expression profile of the genes involved in lipid metabolism and fibroblast growth factor 21 signaling pathway. CONCLUSIONS: This study demonstrated that RPE protected from diet-induced obesity and related metabolic disorders by improving the lipid metabolism and fibroblast growth factor 21 resistance.

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Characterization of fibroblast growth factor 1 in obese children and adolescents

Endocrine Connections ◽

10.1530/ec-18-0141 ◽

2018 ◽

Vol 7 (8) ◽

pp. 932-940 ◽

Cited By ~ 2

Author(s):

Anru Wang ◽

Xueqin Yan ◽

Cai Zhang ◽

Caiqi Du ◽

Wenjun Long ◽

...

Keyword(s):

Insulin Resistance ◽

Weight Loss ◽

Growth Factor ◽

Children And Adolescents ◽

Fibroblast Growth Factor ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Fibroblast Growth ◽

Obese Children ◽

Increased Risk

Background Fibroblast growth factor 1 (FGF1) can regulate glucose and lipid metabolism in obese mice. Serum FGF1 has increased in type 2 diabetes mellitus adults and correlated with BMI. This study aimed to indicate conventional weight loss effects on FGF1 in obese children and adolescents. Materials and methods Clinical and metabolic parameters of 88 lean and obese individuals (ages 5–15 years) and 39 obese individuals followed with 6 months of lifestyle intervention were collected. Serum FGF1 levels were detected through enzyme-linked immunosorbent assays. Results FGF1 levels were increased in obese individuals. Serum FGF1 levels were significantly correlated with BMI and waist circumferences (r = 0.377, P = 0.012; r = 0.301, P = 0.047, respectively). Multivariate stepwise linear regression analyses showed that FGF1 levels were significantly correlated with HbA1c and HOMA-IR (β = 0.371, P = 0.008; β = 0.323, P = 0.021, respectively). Weight loss (2.3 ± 0.1 kg) was accompanied by a significant reduction of circulating FGF1 levels (7.2 ± 0.4 pg/mL). Changes in FGF1 were significantly correlated with changes in fasting glucose, HOMA-IR and low-density lipoprotein cholesterol (β = 0.277, P = 0.020; β = 0.474, P < 0.001; β = 0.320, P = 0.008, respectively). Conclusion FGF1 was related to increased risk of insulin resistance in obese children and adolescents. Serum FGF1 reduced after weight loss in obese individuals and was associated with the improvement of insulin resistance. Changes in serum FGF1 were more correlated with insulin resistance than changes in obesity per se.

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Effects of anti-inflammatory treatment on fibroblast growth factor-21 in obesity and metabolic syndrome

Endocrine Abstracts ◽

10.1530/endoabs.63.gp44 ◽

2019 ◽

Author(s):

Fahim Ebrahimi ◽

Sandrine Urwyler ◽

Matthias Betz ◽

Emanuel Christ ◽

Philipp Schuetz ◽

...

Keyword(s):

Metabolic Syndrome ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Fibroblast Growth Factor 21 ◽

Fibroblast Growth ◽

Anti Inflammatory

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Effects of gender and body weight on fibroblast growth factor 23 responsiveness to estimated dietary phosphorus

The Journal of Medical Investigation ◽

10.2152/jmi.63.58 ◽

2016 ◽

Vol 63 (1.2) ◽

pp. 58-62 ◽

Cited By ~ 1

Author(s):

Hiroyuki Ohta ◽

Masae Sakuma ◽

Akitsu Suzuki ◽

Yuuka Morimoto ◽

Makoto Ishikawa ◽

...

Keyword(s):

Body Weight ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Fibroblast Growth Factor 23 ◽

Fibroblast Growth ◽

Dietary Phosphorus

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Abstract P088: Obesity, Hypertension and Insulin Resistance in a Primary Pediatric Setting in Southern Italy

Circulation ◽

10.1161/circ.129.suppl_1.p088 ◽

2014 ◽

Vol 129 (suppl_1) ◽

Author(s):

Giampaolo De Filippo ◽

Domenico Rendina ◽

Domenico Viggiano ◽

Antonio Fasolino ◽

Paola Sabatini ◽

...

Keyword(s):

Insulin Resistance ◽

Metabolic Syndrome ◽

Body Weight ◽

Children And Adolescents ◽

Diagnostic Criteria ◽

Southern Italy ◽

Serum Insulin ◽

High Density Lipoproteins ◽

Obese Children ◽

Campania Region

Background: Obesity is the main risk factor for essential hypertension (EH) in childhood. The O.Si.Me. study (Obesity and Metabolic Syndrome in children and adolescents) evaluated the prevalence of metabolic syndrome (MetS) and its constitutive traits in a sample of obese children and adolescents living in Campania, southern Italy. Patients and methods: Four hundred and fifteen children and adolescents consecutively referred to the National Health Service participating Outpatient Clinics for minor health problems and found to have a Body Mass Index (BMI) Z-score > 2.0 were enrolled in the study. The entire sample was screened for MetS, which was defined as the presence of at least 2 of the following alterations in addition to obesity: fasting hyperglycemia, low levels of high-density lipoproteins cholesterol, hypertriglyceridemia, and EH. The present analysis evaluated the clinical characteristics of the O.Si.Me subgroup of EH participants (systolic and/or diastolic BP ≥ 95 th percentile for age, gender and height) as compared with normotensive participants. Results: The prevalence of EH in the O.Si.Me population was 23.6 % (98/415, 48M and 50F.) and two-thirds of the EH participants met the MetS diagnostic criteria. The EH participants featured serum insulin and HOMA-IR levels significantly higher compared with normotensive ones (11.6±0.6 vs. 9.5±0.4 μIU/ml, p = 0.014; 2.6±0.1 vs. 2.2±0.1, p = 0.028 for insulin and HOMA-IR, respectively). These differences were common to boys and girls and remained significant after correction for age, pubertal stage, body weight, length, BMI, gestational age at birth, duration of breastfeeding and anthropometric parental parameters. Accordingly, children and adolescents with EH had a a relative risk of being insulin resistant (defined as a HOMA-IR ≥2.5) significantly greater compared to those without. Moreover, they exhibited higher serum creatinine levels (53.8±7.1 vs. 35.4±6.8 μmol/l, p=0.025) accounting for gender and body weight. Conclusions: More than a quarter of obese children and adolescents meet the diagnostic criteria for EH in the Campania region in southern Italy. These obese boys and girls have an increased prevalence of insulin resistance and apparently an initial reduction in renal function compared with obese children and adolescents with normal BP.

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