Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Abstract Background Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results Totally, 26 odorant-binding proteins (OBP) genes, 19 chemosensory proteins (CSP) genes, 55 odorant receptors (OR) genes and 20 ionotropic receptors (IR) genes were identified from the C. pinicolalis antennae transcriptome and most of them were olfactory genes, amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we strongly recommended the GAPDH or RP49 to verify gene expression for larvae development and RP49 or RPL13 for adult tissues. Conclusions Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be a supportive for pest management studies in future.

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Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

10.21203/rs.2.9614/v1 ◽

2019 ◽

Author(s):

Dapeng Jing ◽

Tiantao Zhang ◽

Shuxiong Bai ◽

Kanglai He ◽

Sivaprasath Prabu ◽

...

Keyword(s):

Gene Expression ◽

Odorant Receptors ◽

Masson Pine ◽

Odorant Binding Proteins ◽

Starting Point ◽

Yellow Peach Moth ◽

Or Genes ◽

Odorant Binding ◽

First Time ◽

Ir Genes

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Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

10.21203/rs.2.9614/v3 ◽

2020 ◽

Author(s):

Dapeng Jing ◽

Tiantao Zhang ◽

Shuxiong Bai ◽

Kanglai He ◽

Sivaprasath Prabu ◽

...

Keyword(s):

Gene Expression ◽

Ribosomal Protein ◽

Odorant Receptor ◽

Ionotropic Receptor ◽

Masson Pine ◽

Chemosensory Protein ◽

Starting Point ◽

Dehydrogenase Gene ◽

Yellow Peach Moth ◽

Or Genes

Abstract Background: Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms , especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results: Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis . The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis . Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions: Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis , which might be supportive for pest management studies in future.

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Sex- and tissue-specific transcriptome analyses and expression profiling of olfactory-related genes in Ceracris nigricornis Walker (Orthoptera: Acrididae)

BMC Genomics ◽

10.1186/s12864-019-6208-x ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 4

Author(s):

Hao Yuan ◽

Huihui Chang ◽

Lina Zhao ◽

Chao Yang ◽

Yuan Huang

Keyword(s):

Olfactory System ◽

Binding Proteins ◽

Expression Profiles ◽

Odorant Receptors ◽

Evolutionary Trend ◽

Odorant Binding Proteins ◽

Functional Studies ◽

Host Seeking ◽

Odorant Binding ◽

First Time

Abstract Background The sophisticated insect olfactory system plays an important role in recognizing external odors and enabling insects to adapt to environment. Foraging, host seeking, mating, ovipositing and other forms of chemical communication are based on olfaction, which requires the participation of multiple olfactory genes. The exclusive evolutionary trend of the olfactory system in Orthoptera insects is an excellent model for studying olfactory evolution, but limited olfaction research is available for these species. The olfactory-related genes of Ceracris nigricornis Walker (Orthoptera: Acrididae), a severe pest of bamboos, have not yet been reported. Results We sequenced and analyzed the transcriptomes from different tissues of C. nigricornis and obtained 223.76 Gb clean data that were assembled into 43,603 unigenes with an N50 length of 2235 bp. Among the transcripts, 66.79% of unigenes were annotated. Based on annotation and tBLASTn results, 112 candidate olfactory-related genes were identified for the first time, including 20 odorant-binding proteins (OBPs), 10 chemosensory-binding proteins (CSPs), 71 odorant receptors (ORs), eight ionotropic receptors (IRs) and three sensory neuron membrane proteins (SNMPs). The fragments per kilobase per million mapped fragments (FPKM) values showed that most olfactory-related differentially expressed genes (DEGs) were enriched in the antennae, and these results were confirmed by detecting the expression of olfactory-related genes with quantitative real-time PCR (qRT-PCR). Among these antennae-enriched genes, some were sex-biased, indicating their different roles in the olfactory system of C. nigricornis. Conclusions This study provides the first comprehensive list and expression profiles of olfactory-related genes in C. nigricornis and a foundation for functional studies of these olfactory-related genes at the molecular level.

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Identification and Expression Profiling of Peripheral Olfactory Genes in the Parasitoid Wasp Aphidius ervi (Hymenoptera: Braconidae) Reared on Different Aphid Hosts

Insects ◽

10.3390/insects10110397 ◽

2019 ◽

Vol 10 (11) ◽

pp. 397

Author(s):

Gabriel I. Ballesteros ◽

Daniela A. Sepúlveda ◽

Christian C. Figueroa

Keyword(s):

Binding Proteins ◽

Host Preference ◽

Odorant Receptors ◽

Aphidius Ervi ◽

Host Acceptance ◽

Odorant Binding Proteins ◽

Expression Levels ◽

Host Fidelity ◽

Chemosensory Genes ◽

Odorant Binding

Generalist parasitoids of aphids, such as the wasp Aphidius ervi, display significant differences in terms of host preference and host acceptance, depending on the host on which they developed (natal host), which is preferred over a non-natal host, a trait known as host fidelity. This trait allows females to quickly find hosts in heterogeneous environments, a process mediated by chemosensory/olfactory mechanisms, as parasitoids rely on olfaction and chemical cues during host selection. Thus, it is expected that proteins participating in chemosensory recognition, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) would play a key role in host preference. In this study, we addressed the effect of parasitoid reciprocal host switching between two aphid hosts (Sitobion avenae and Acyrthosiphon pisum) on the expression patterns of chemosensory genes in the wasp A. ervi. First, by using a transcriptomic approach based on RNAseq of A. ervi females reared on S. avenae and A. pisum, we were able to annotate a total of 91 transcripts related to chemoperception. We also performed an in-silico expression analysis and found three OBPs and five ORs displaying different expression levels. Then, by using qRT-PCR amplification, we found significant differences in the expression levels of these eight genes when the parasitoids were reciprocally transplanted from S. avenae onto A. pisum and vice versa. This suggests that the expression levels of genes coding for odorant receptors and odorant-binding proteins would be regulated by the specific plant–aphid host complex where the parasitoids develop (maternal previous experience) and that chemosensory genes coding for olfactory mechanisms would play a crucial role on host preference and host acceptance, ultimately leading to the establishment of host fidelity in A. ervi parasitoids.

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Gene Expression Profiles of Odorant Binding Proteins inGlossina brevipalpis(Diptera: Glossinidae)

Journal of Entomological Science ◽

10.18474/jes17-103.1 ◽

2018 ◽

Vol 53 (4) ◽

pp. 467-477 ◽

Cited By ~ 1

Author(s):

Mary Murithi ◽

Rosaline Macharia ◽

Esther Magiri ◽

Steven Nyanjom ◽

Daniel Masiga

Keyword(s):

Gene Expression ◽

Binding Proteins ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Odorant Binding Proteins ◽

Odorant Binding

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Gene expression profile of Aedes aegypti females in courtship and mating

Scientific Reports ◽

10.1038/s41598-019-52268-5 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Diego Peres Alonso ◽

Melina Campos ◽

Heitor Troca ◽

Rafael Kunii ◽

Frédéric Tripet ◽

...

Keyword(s):

Gene Expression ◽

Aedes Aegypti ◽

Pathogen Transmission ◽

Odorant Binding Proteins ◽

Control Approach ◽

Transcriptional Changes ◽

Urban Scenario ◽

Odorant Binding ◽

Mating Conditions ◽

Transcriptomic Change

Abstract Aedes aegypti is the most synanthropic and anthropophilic mosquito of Culicidae. This species always cohabits with humans and is extremely opportunistic. Vector dispersal is directly related to the ability of the females on successfully finding a mate in a generally patchy urban scenario. In the present work, we investigate transcriptional changes in Ae. aegypti females during the courtship process and after mating. We observe a substantial alteration in gene expression triggered just upon contact with Ae. aegypti males, which in turn was not fully correlated to the changes triggered by the contact. After analysing shared significant differentially regulated genes between conspecific contact and insemination, the major part of the observed transcriptomic change triggered by contact is reversed after mating, indicating an intermediary situation between naive and mating conditions that we hypothesize to be crucial for mating success. Upon contact, several chemosensory related genes are repressed, especially odorant binding proteins. Most of these genes return to higher expression rates after mating. None of these genes are significantly regulated by the encounter of a different species, Aedes albopictus. The results presented here might be applied to an innovative control approach focusing on the semiochemical systems of mosquitoes in an effort to disrupt undesirable host–insect interaction to reduce the risk of pathogen transmission to humans.

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Genome-wide survey of odorant-binding proteins in the dwarf honey bee Apis florea

10.1101/2021.02.25.432941 ◽

2021 ◽

Author(s):

Bhavika Mam ◽

Snehal Karpe ◽

Ramanathan Sowdhamini

Keyword(s):

Honey Bee ◽

Binding Proteins ◽

Odorant Receptors ◽

Apis Florea ◽

Odor Coding ◽

Complex Behaviour ◽

Odorant Binding Proteins ◽

Eusocial Insects ◽

Genome Wide ◽

Odorant Binding

AbstractOdorant binding proteins (OBPs) in insects bind to volatile chemical cue and help in their binding to odorant receptors. The odor coding hypothesis states that OBPs may bind with specificity to certain volatiles and aid the insect in various behaviours. Honeybees are eusocial insects with complex behaviour that requires olfactory inputs. Here, we have identified and annotated odorant binding proteins from the genome of the dwarf honey bee, Apis florea using an exhaustive homology-based bioinformatic pipeline and analyzed the evolutionary relationships between the OBP subfamilies. Our study suggests that Minus-C subfamily may have diverged from the Classic subfamily of odorant binding proteins in insects.

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Gene Expression and Functional Analyses of Odorant Receptors in Small Hive Beetles (Aethina tumida)

International Journal of Molecular Sciences ◽

10.3390/ijms21134582 ◽

2020 ◽

Vol 21 (13) ◽

pp. 4582

Author(s):

Yuanzhen Liu ◽

Alexis Beaurepaire ◽

Curtis W. Rogers ◽

Dawn Lopez ◽

Jay D. Evans ◽

...

Keyword(s):

Expression Patterns ◽

Host Finding ◽

Odorant Receptors ◽

Aethina Tumida ◽

Colony Odor ◽

Or Gene ◽

Functional Analyses ◽

Or Genes ◽

Small Hive Beetles ◽

First Time

Olfaction is key to many insects. Odorant receptors (ORs) stand among the key chemosensory receptors mediating the detection of pheromones and kairomones. Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies and olfactory cues are especially important for host finding. However, how interactions with their hosts may have shaped the evolution of ORs in the SHB remains poorly understood. Here, for the first time, we analyzed the evolution of SHB ORs through phylogenetic and positive selection analyses. We then tested the expression of selected OR genes in antennae, heads, and abdomens in four groups of adult SHBs: colony odor-experienced/-naive males and females. The results show that SHBs experienced both OR gene losses and duplications, thereby providing a first understanding of the evolution of SHB ORs. Additionally, three candidate ORs potentially involved in host finding and/or chemical communication were identified. Significantly different downregulations of ORs between the abdomens of male and female SHBs exposed to colony odors may reflect that these expression patterns might also reflect other internal events, e.g., oviposition. Altogether, these results provide novel insights into the evolution of SHB ORs and provide a valuable resource for analyzing the function of key genes, e.g., for developing biological control. These results will also help in understanding the chemosensory system in SHBs and other beetles.

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Identification of candidate chemosensory genes of Ophraella communa LeSage (Coleoptera: Chrysomelidae) based on antennal transcriptome analysis

Scientific Reports ◽

10.1038/s41598-019-52149-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Chao Ma ◽

Chenchen Zhao ◽

Shaowei Cui ◽

Yan Zhang ◽

Guangmei Chen ◽

...

Keyword(s):

Host Plants ◽

Odorant Receptors ◽

Oviposition Site Selection ◽

Odorant Binding Proteins ◽

Chemosensory Genes ◽

Olfactory Systems ◽

Ophraella Communa ◽

Polymerase Chain ◽

Odorant Binding ◽

Generation Sequencing

Abstract Antennal olfaction plays a key role in insect survival, which mediates important behaviors like host search, mate choice, and oviposition site selection. As an oligophagous insect, olfaction is extremely important for Ophraella communa to locate host plants. However, information on the olfactory genes has been lacking in O. communa. Using next generation sequencing, we assembled the antennal transcriptome of O. communa and first reported the major chemosensory genes necessary for olfaction in this species. In this study, a total 105 candidate chemosensory genes were identified in O. communa antennae, including 25 odorant-binding proteins (OBPs), 11 chemosensory proteins (CSPs), four sensory neuron membrane proteins (SNMPs), 30 odorant receptors (ORs), 18 ionotropic receptors (IRs), and 17 gustatory receptors (GRs). We also identified full-length sequences of the highly conserved ORco and IR8a/25a family in O. communa. In addition, the expression profile of 15 ORs and four OBPs were validated by quantitative real-time polymerase chain reaction (qPCR). We found that OcomOR2/4/19 and OcomOBP19/20 had a biased expression in male antennae, and OcomOR8 had a biased expression in the female antennae. This large number of chemosensory genes handled by homology analysis and qPCR results will provide the first insights into molecular basis for the olfactory systems of O. communa as well as advance our understanding of olfactory mechanisms in Coleoptera.

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De novo assembly and identification of antennal transcriptome reveals an olfactory system in Heortia vitessoides (Lepidoptera: Crambidae)

10.7287/peerj.preprints.3433 ◽

2017 ◽

Author(s):

Jie Cheng ◽

Chun-Yan Wang ◽

Zi-Hao Lyu ◽

Jing-Xiang Chen ◽

Tong Lin

Keyword(s):

Phylogenetic Trees ◽

De Novo ◽

Odorant Receptors ◽

Sequencing Technology ◽

Aquilaria Sinensis ◽

Model Species ◽

Neuron Membrane ◽

Odorant Binding Proteins ◽

Olfactory Systems ◽

Odorant Binding

Olfactory systems in insects are used to detect volatile chemical odors, and play crucial roles in survival, reproduction, and mediating key behaviors. Here, RNA sequencing technology was used to sequence and assemble the antennal transcriptome of Heortia vitessoides, a defoliating pest in Aquilaria sinensis (Loureiro) Sprenger forests and a non-model species with no genomic resources. Analysis of the transcriptome of female and male antennae generated 22.16 gigabases of genomic data, from which 52,383 unigenes were assembled. We identified 80 candidate olfactory genes: eight for odorant binding proteins (OBPs), 14 for chemosensory proteins (CSPs), 35 odorant receptors (ORs), 18 ionotropic receptors (IRs), three gustatory receptors (GRs), and two for sensory neuron membrane proteins (SNMPs). Furthermore, phylogenetic trees and fragments per kilobase of transcript per million fragments mapped (FPKM) were used to analyze these olfactory genes. This study is the first comprehensive antennal transcriptome analysis for H. vitessoides, and these novel olfactory genes will increase understanding of the molecular mechanism of chemoreception and further contribute to exploring strategies to manage this insect.

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