Bone Regeneration in Rat Using a PCL/gelatin/Nanoclay Nanocomposite Scaffold Containing Silybin

Abstract Bone cells need solid structures like the extracellular matrix (ECM) for healing injured areas. Finding appropriate materials and fabrication processes for the scaffold is a challenge in tissue engineering. In this study, 3-D porous scaffold was made of Polycaprolactone/Gelatin/Nanoclay (PCL/GNF/NC) and different dosages of silybin (Sil) were loaded by a combination of electrospinning and thermal-induced phase separation (TIPS) techniques. Different experiments like assessing surface morphology, porosity, compressive strength, water contact angle, degradation rate, releasing profile, hemolysis, and cell proliferation were done to assess attributes of fabricated scaffolds. For in vivo evaluation, the calvaria defect model in rats was used and the result was evaluated by histological analysis. Based on the results, the porosity of scaffolds was in the range of 70-90%, and samples containing silybin had lower compress strength and contact angle and higher degradation rate in comparison with samples without silybin. The results showed that PCL/GNF/NC/Sil1% had better cell proliferation bone healing than other studied groups. The results of this study can be considered for further researches to assess the effect of silybin in bone defect treatment.

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Osteoconductivity of Superhydrophilic Ti- and Zr-Alloy for Biomedical Application

Materials Science Forum ◽

10.4028/www.scientific.net/msf.879.2524 ◽

2016 ◽

Vol 879 ◽

pp. 2524-2527

Author(s):

Masazumi Okido ◽

Kensuke Kuroda

Keyword(s):

Contact Angle ◽

Water Contact Angle ◽

Strong Influence ◽

Biomedical Application ◽

Hydrophobic Surface ◽

Hydrophilic Surface ◽

Water Contact ◽

Zr Alloy ◽

Zr Alloys

Surface hydrophilicity is considered to have a strong influence on the biological reactions of bone-substituting materials. However, the influence of a hydrophilic or hydrophobic surface on the osteoconductivity is not completely clear. In this study, we produced super-hydrophilic and hydrophobic surface on Ti-and Zr-alloys. Hydrothermal treatment at 180 oC for 180 min. in the distilled water and immersion in x5 PBS(-) brought the super-hydrophilic surface (water contact angle < 10 (deg.)) and heat treatment of as-hydrothermaled the hydrophobic surface. The osteoconductivity of the surface treated samples with several water contact angle was evaluated by in vivo testing. The surface properties, especially water contact angle, strongly affected the osteoconductivity and protein adsorbability, and not the surface substance.

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Synthesis and Characterization of Biodegradable Polyester/Polyether WPU As The Environmental Protection Coating

10.21203/rs.3.rs-428884/v1 ◽

2021 ◽

Author(s):

Guangfeng Wu ◽

Xin Song ◽

ZhiHui Yang ◽

Ying Chun Li ◽

HuiXuan Zhang

Keyword(s):

Thermal Stability ◽

Contact Angle ◽

Water Contact Angle ◽

Degradation Rate ◽

Soft Segment ◽

Buffer Solution ◽

Water Contact ◽

Elongation At Break ◽

Protection Coating

Abstract Polyester diol PCL and PBA, polyether diol PTMG and polycarbonate diol PCDL were used as components of WPU soft segment, respectively. Polyether PTMG-WPU has the worst hydrolytic property and the highest thermal stability. The maximum degradation rate temperature Tmax is 407.8°C, the water contact angle reaches 89.5°. Traditional polyester PCL-WPU shows the strongest hydrolysis performance, the smallest water contact angle, only 71.7°, the water absorption rate of 72 hours at room temperature is as high as 26.7%. However, the thermal stability of PCL-WPU is lower, the soft segment Tg is -52.3°C, and Tmax is only 333.7°C, but the mechanical propertie of which is the best, the tensile strength is 58.3 MPa, and the elongation at break reaches 857.9%. The most important thing is that the structure of polyester PCL-WPU is more easily destroyed by lipase and water molecules. The acidic products produced after hydrolysis will further promote the degradation of polyester. Therefore, compared with other WPUs, PCL -WPU has the best biodegradability and the most obvious degradation effect under the same conditions. The degradation rate of PTMG-WPU after 30 days of degradation in 0.6% lipase PBS buffer solution and soil was only 4.2% and 2.3%, while the highest degradation rate of traditional polyester PCL-WPU reached 41.7% and 32.0%, respectively. In addition, polycarbonate PCDL-WPU has the highest hardness, reaching 95.5 HD. But its other performances are lower than PCl-WPU.

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A Porous Hydroxyapatite/Gelatin Nanocomposite Scaffold for Bone Tissue Repair: In Vitro and In Vivo Evaluation

Journal of Biomaterials Science Polymer Edition ◽

10.1163/156856211x617713 ◽

2012 ◽

Vol 23 (18) ◽

pp. 2353-2368 ◽

Cited By ~ 42

Author(s):

Mahmoud Azami ◽

Shima Tavakol ◽

Ali Samadikuchaksaraei ◽

Mehran Solati Hashjin ◽

Nafiseh Baheiraei ◽

...

Keyword(s):

Bone Tissue ◽

Tissue Repair ◽

In Vivo Evaluation ◽

Porous Hydroxyapatite ◽

Nanocomposite Scaffold

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Osteoconductivity of Hydrothermal-Treated Valve Metals

Materials Science Forum ◽

10.4028/www.scientific.net/msf.783-786.1298 ◽

2014 ◽

Vol 783-786 ◽

pp. 1298-1302 ◽

Cited By ~ 1

Author(s):

Kensuke Kuroda ◽

Mansjur Zuldesmi ◽

Masazumi Okido

Keyword(s):

Contact Angle ◽

Water Contact Angle ◽

Surface Coatings ◽

Tissue Formation ◽

Hydrophilic Surface ◽

Bioactive Substances ◽

Water Contact ◽

Metallic Biomaterials ◽

Valve Metals

Anti-corroded valve metals, such as Ti, Nb, Ta, and Zr have been used as metallic biomaterials. However, as untreated surfaces, they do not have high osteoconductivity, and surface coatings with bioactive substances are needed for the implantation into the bone. Surface property, especially hydrophilicity, is considered to have a strong influence on the biological reactions. However, the influence of a hydrophilic surface on osteoconductivity is not completely clear. In this study, we produced super-hydrophilic surface on valve metals (Ti, Nb, Ta and Zr) using a hydrothermal treatment at 180 °C for 180 min. in the distilled water, and then the treated samples were stored in 5PBS(-). This maintained water contact angle less than 10 (deg.) in an apparent. The osteoconducivity of super-hydrophilic treated metals was evaluated with in vivo tests. The hard tissue formation on the samples increased with decreasing the water contact angle. That is to say that super-hydrophilic valve metals without coating of bioactive substances had high osteoconductivity, and the surface properties strongly affected on the osteoconductivity.

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Osteoconductivity of Superhydrophilic Anodized TiO2 Films Using Hydrothermal Treatment

Materials Science Forum ◽

10.4028/www.scientific.net/msf.783-786.1332 ◽

2014 ◽

Vol 783-786 ◽

pp. 1332-1336

Author(s):

Masazumi Okido ◽

Kensuke Kuroda

Keyword(s):

Contact Angle ◽

Hydrothermal Treatment ◽

Water Contact Angle ◽

Inorganic Ions ◽

Tissue Formation ◽

Hard Tissue ◽

Water Contact ◽

Long Time

In this study, we conferred superhydrophilic properties on anodized TiO2 coatings using a hydrothermal treatment, and developed a method to maintain this surface until implantation. The osteoconductivity of these coatings was evaluated with in vivo tests. A hydrothermal treatment made the surface of as-anodized samples more hydrophilic, up to a water contact angle of 13 deg. Storage in PBS(-) led to a reduction in the water contact angle, because of the adsorption of the inorganic ions in the solution, and the sample retained its high hydrophilicity for a long time. As the water contact angle decreased, the hard tissue formation ratio increased continuously up to 58 %, which was about four times higher than the hard tissue formation ratio on as-polished Ti.

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In Vitro and In Vivo Evaluation of 1-(3 Dimethylaminopropyl)-3-Ethyl Carbodiimide (EDC) Cross-Linked Gum Arabic–Gelatin Composite as an Ideal Porous Scaffold for Tissue Engineering

Biomaterials in Orthopaedics and Bone Regeneration - Materials Horizons: From Nature to Nanomaterials ◽

10.1007/978-981-13-9977-0_9 ◽

2019 ◽

pp. 131-145

Author(s):

Boby T. Edwin ◽

H. Dhanya ◽

Prabha D. Nair ◽

Moustapha Kassem

Keyword(s):

Tissue Engineering ◽

Porous Scaffold ◽

Gum Arabic ◽

In Vivo Evaluation

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In vivo evaluation of bending strengths and degradation rates of different magnesium pin designs for oral stapler

Journal of Applied Biomaterials & Functional Materials ◽

10.1177/2280800019836400 ◽

2020 ◽

Vol 18 ◽

pp. 228080001983640

Author(s):

Wenjun Li ◽

Fusong Yuan ◽

Jing Bai ◽

Junyao Cheng ◽

Hongxiang Li ◽

...

Keyword(s):

Magnesium Alloys ◽

Degradation Rate ◽

Bending Strength ◽

Sprague Dawley ◽

In Vivo Evaluation ◽

Cross Sectional ◽

Round Cross Section ◽

Sd Rats ◽

Degradation Rates

Magnesium alloys have been potential biodegradable implants in the areas of bone, cardiovascular system, gastrointestinal tract, and so on. The purpose of this study is to evaluate Mg–2Zn alloy degradation as a potential suture material. The study included Sprague–Dawley (SD) rats in vivo. In 24 male SD rats, tests in the leg muscle were conducted using traditional surgical incision and insertion of magnesium alloys of different designs into the tissue. The material degradation topography, elemental composition, and strength of the pins were analyzed. This paper explores magnesium pins with different cross-sectional shapes and diameters to establish a suitable pin diameter and shape for use as an oral stapler, which must have a good balance of degradation rate and strength. The results showed there were good bending strengths over different degradation periods in groups with diameters of 0.8 mm and 0.5 mm, and no significantly different bending strength between the groups of triangle and round cross-section shapes with same diameter of 0.3 mm, although the degradation rate still needs to be improved.

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