Effect of Different Washing Solutions on Soil Enzyme Activity and Microbial Community in Agricultural Soil Severely Contaminated With Cadmium

Abstract Soil enzyme activities and microbial communities have a good response to the remediation effect of heavy metal-contaminated soils. To evaluate the effect of three commonly used washing agents, ferric chloride (FC), ethylenediamine-tetra- methylenephosphonic acid (EDTMP) and ethylenediamine tetraacetic acid (EDTA) on soil enzyme activities and microbial community in cadmium (Cd)- contaminated agricultural soil collected around the mining area. The soil enzyme activities, microbial community, chemical forms of Cd and some physicochemical properties of the soil washed with different washing solutions were determined after two months incubation. The results showed that the three washing solutions had moderate removal efficiencies (22.83%-37.56%) for Cd in the tested soil and the breakdown product of EDTMP has a certain stabilizing effect on Cd. The geometric mean and the integrated total enzyme activity index showed that soil washing with FC and EDTA were more beneficial to the restoration of biochemical functions than that with EDTMP. The relative abundance of Gemmatimonadetes in the soil washed with EDTA was significantly higher than that with FC and EDTMP. The relative abundance of Firmicutes in the soil washed with EDTMP was significantly increased, and the relative abundance of Bacteroidetes in the soil washed with FC was significantly decreased. Pearson correlation analysis and redundancy analysis (RDA) indicated that the three washing solutions affected soil enzyme activities and microbial community by altering soil nutrient, total Cd concentration and Cd fractions in soils.

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Contrasting Effects of Chinese Fir Plantations of Different Stand Ages on Soil Enzyme Activities and Microbial Communities

Forests ◽

10.3390/f10010011 ◽

2018 ◽

Vol 10 (1) ◽

pp. 11 ◽

Cited By ~ 7

Author(s):

Chaoqun Wang ◽

Lin Xue ◽

Yuhong Dong ◽

Lingyu Hou ◽

Yihui Wei ◽

...

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Relative Abundance ◽

Enzyme Activities ◽

Soil Enzyme ◽

Chinese Fir ◽

Fungal Communities ◽

Stand Age ◽

Forest Age ◽

Soil Enzyme Activities

Soil enzymes and microbial communities are key factors in forest soil ecosystem functions and are affected by stand age. In this study, we studied soil enzyme activities, composition and diversity of bacterial and fungal communities and relevant physicochemical properties at 0–10 cm depth (D1), 10–20 cm depth (D2) and 20–30 cm depth (D3) soil layers in 3-(3a), 6-(6a), 12-(12a), 18-(18a), 25-(25a), 32-(32a) and 49-year-old (49a) Chinese fir plantations to further reveal the effects of stand age on soil biotic properties. Spectrophotometry and high-throughput sequencing was used to assess the soil enzyme activity and microbial community composition and diversity of Chinese fir plantation of different stand ages, respectively. We found that soil catalase activity increased as the stand age of Chinese fir plantations increased, whereas the activities of urease, sucrase and β-glucosidase in 12a, 18a and 25a were lower than those in 6a, 32a and 49a. Shannon and Chao1 indices of bacterial and fungal communities first decreased gradually from 6a to 18a or 25a and then increased gradually from 25a to 49a. Interestingly, the sucrase and β-glucosidase activities and the Shannon and Chao1 indices in 3a were all lower than 6a. We found that the relative abundance of dominant microbial phyla differed among stand ages and soil depths. The proportion of Acidobacteria first increased and then decreased from low forest age to high forest age, and its relative abundance in 12a, 18a and 25a were higher than 3a, 32a and 49a, but the proportion of Proteobacteria was opposite. The proportion of Ascomycota first decreased and then increased from 6a to 49a, and its relative abundance in 12a, 18a and 25a was lower than 3a, 6a, 32a and 49a. Our results indicate that soil enzyme activities and the richness and diversity of the microbial community are limited in the middle stand age (from 12a to 25a), which is important for developing forest management strategies to mitigate the impacts of degradation of soil biological activities.

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Inoculation of Pseudomonas sp. GHD-4 and mushroom residue carrier increased the soil enzyme activities and microbial community diversity in Pb-contaminated soils

Journal of Soils and Sediments ◽

10.1007/s11368-018-2111-3 ◽

2018 ◽

Vol 19 (3) ◽

pp. 1064-1076 ◽

Cited By ~ 6

Author(s):

Sumei Yu ◽

Xin Bai ◽

Jinsong Liang ◽

Yingnan Wei ◽

Siqi Huang ◽

...

Keyword(s):

Microbial Community ◽

Enzyme Activities ◽

Contaminated Soils ◽

Soil Enzyme ◽

Community Diversity ◽

Pseudomonas Sp ◽

Soil Enzyme Activities ◽

Microbial Community Diversity

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Change Analysis of Enzyme Activity under Different Conditions of Film Remnant in Soil

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.518-523.39 ◽

2012 ◽

Vol 518-523 ◽

pp. 39-43

Author(s):

Xiao Guang Zhao ◽

Yuan Yuan Guan ◽

Wen Yu Huang

Keyword(s):

Enzyme Activity ◽

Enzyme Activities ◽

Scientific Basis ◽

Soil Enzyme ◽

Soil Microorganism ◽

Soil Enzyme Activities ◽

Change Analysis ◽

Soil Material ◽

The Relationship

In this paper, simulated experiments were performed in pots by using soil materials in different conditions of film remnant. Based on the research on soil microorganism quantity trends of soil enzyme activities were analyzed systematically: soil without film remnant, soil with film remnant for 5, 10, 15 and 20 years. By analyzing crop progress, the relationship with soil material was studied, in order to provide scientific basis for the variation laws between different conditions of film remnant and the activity of soil enzyme.

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Rhizospheric soil enzyme activities and phytominimg potential of Aeluropus lagopoides and Cyperus conglomeratus growing in contaminated soils at the banks of artificial lake of reclaimed wastewater

International Journal of Phytoremediation ◽

10.1080/15226514.2017.1319326 ◽

2017 ◽

Vol 19 (11) ◽

pp. 1017-1022

Author(s):

Zahid Khorshid Abbas

Keyword(s):

Enzyme Activities ◽

Contaminated Soils ◽

Soil Enzyme ◽

Rhizospheric Soil ◽

Soil Enzyme Activities ◽

Reclaimed Wastewater ◽

Artificial Lake ◽

Aeluropus Lagopoides

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Effects of Cd or/and Pb on soil enzyme activities and microbial community structure

Ecological Engineering ◽

10.1016/j.ecoleng.2011.07.002 ◽

2011 ◽

Vol 37 (11) ◽

pp. 1889-1894 ◽

Cited By ~ 109

Author(s):

Jing Pan ◽

Long Yu

Keyword(s):

Community Structure ◽

Microbial Community ◽

Microbial Community Structure ◽

Enzyme Activities ◽

Soil Enzyme ◽

Soil Enzyme Activities

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The response of soil enzyme activity to seasonal and microtopographical variations in the sedge peatlands in Changbai Mountain, China

10.5194/egusphere-egu2020-22531 ◽

2020 ◽

Author(s):

Ming Wang

Keyword(s):

Enzyme Activity ◽

Seasonal Variation ◽

Enzyme Activities ◽

Soil Enzyme ◽

Changbai Mountain ◽

Soil Enzyme Activity ◽

Small Scale ◽

P Availability ◽

Soil Enzyme Activities ◽

Significant Difference

Hummock-hollow microtopography is a common feature in northern peatlands. It creates microsites of variable hydrology, vegetation, and soil biogeochemistry, thus affect soil C cycling in peatlands at the local scale. This study investigated effects of microtopography on soil enzyme (&#946;-1,4-glucosidase (&#946;G), &#946;-1,4-N-acetylglucosaminidase (NAG), acid phosphatase (AP) and peroxidase (PER)) activities and environment variables as well as their relationships in a typical sedge peatland in Changbai Mountain, northeast of China. Our results showed that the enzyme activities in the sedge peatland significantly varied across seasons and microtopographical positions. Soil enzyme activities in hummocks exhibited more obvious seasonal variation than hollows, with the &#946;G, AP and PER activities presented a distinct valley in summer and the maximum values occurred in Spring or Autumn. Soil hydrolase (&#946;G, NAG and AP) activities in hummocks were significantly higher compared to hollows, while soil oxidase (PER enzyme) activity in hollows was higher than hummocks. The NMDS analysis revealed that the influence degree of microtopography on the enzyme activities was higher than that of seasonal variation. Redundancy analysis (RDA) indicated that the variations of soil enzyme activities in the peatland were related to environmental variables, especially to water table depth (WTD), soil temperature (ST), SOC, N availability and P availability. Furthermore, correlation analysis showed that the three hydrolase (BG, NAG and AP) activities were positively correlated with soil TN, SOC and C/N, and negatively correlated with WTD and TP. On the contrast, the PER activities were positively correlated with TP, and negatively correlated with ST, SOC and C/N. The present study demonstrated that small scale topographic heterogeneity created by hummock cause habitat heterogeneity and thus lead to significant difference of soil enzyme activity between hummock and hollow in the sedge peatlands. This finding provides further evidence of the importance of peatland microtopography to C cycling and has direct implications for scaling biogeochemical processes to the ecosystem level.

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