Indole Acetic Acid Producing And Phosphate Solubilizing Bacteria Native To Kenyan Soils Promote Growth of Common Bean (Phaseolus Vulgaris L.)

2021 ◽  
Author(s):  
Hezekiah Korir ◽  
Nancy W. Mungai ◽  
Victor W. Wasike
Keyword(s):  
Acetic Acid ◽  
Common Bean ◽  
Indole Acetic Acid ◽  
Shoot Biomass ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Rhizobium Phaseoli ◽  
Phosphate Solubilizing ◽  
Set Up

Abstract Use of phosphate solubilizing bacteria (PSB) and rhizobia can have a positive effect on the growth of common bean. This study aimed at determining the mechanisms of action of native bacterial strains; and to determine their effect in enhancing growth of common bean. The strains were screened for their ability to solubilize insoluble inorganic phosphates and production of indole acetic acid in vitro. A greenhouse experiment was set up to evaluate the response of common bean to inoculation with selected bacterial strains. Six of the bacterial isolates tested showed a positive result for IAA production. Rhizobium pusense showed the greatest solubilization efficiency of 648 followed by Bacillus megaterium (322.3) and Rhizobium phaseoli (308.7). Inoculation of common bean with Rhizobia and PSB had a significant effect on the number of nodules per plant. The highest shoot biomass was observed when Rhizobium phaseoli was co-inoculated with P. polymyxa (4.3g plant-1) compared to the single Rhizobium phaseoli inoculation (1.14 g plant-1). The shoot tissue nitrogen and phosphorous concentration was increased as a results of co-inoculation up to 32.5% and 75.4% respectively. Therefore, tested bacterial strains have great potential in being formulated and used as biofertilizers that can be evaluated under varying field conditions.

scholarly journals Isolation and screening for plant growth-promoting (PGP) actinobacteria from Araucaria angustifolia rhizosphere soil

2010 ◽  
Vol 67 (6) ◽  
pp. 743-746 ◽  
Author(s):  
Rafael Leandro Figueiredo de Vasconcellos ◽  
Mylenne Calciolari Pinheiro da Silva ◽  
Carlos Marcelo Ribeiro ◽  
Elke Jurandy Bran Nogueira Cardoso
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Culture Media ◽  
Araucaria Angustifolia ◽  
In Vitro Production ◽  
Soil Ecosystems ◽  
Plant Growth Promoting ◽  
Phosphate Solubilizing ◽  
Vitro Production

Actinobacteria are capable of playing several different roles in soil ecosystems. These microorganisms affect other organisms by producing secondary metabolites and are responsible for the degradation of different complex and relatively recalcitrant organic compounds. In our survey of actinobacteria isolated from the rhizosphere of Araucaria angustifolia, five culture media (AI, WYE, YCED, MSSC and LNMS) were compared for their effectiveness in isolating these microorganisms. When summing up all the isolates randomly obtained, we got 103 isolates. After isolation, the phosphate-solubilizing ability and the "in vitro" production of indole-acetic acid and chitinases were evaluated. The AI medium was ineffective for actinobacteria isolation, when it was compared with the other four culture media. Indole-acetic acid and chitinase were produced by respectively 36% and 24% of the strains tested. However, only 2% of the 103 strains presented some phosphate-solubilizing ability. These results demonstrate the biotechnological potential of these microorganisms.

scholarly journals Selected Rhizosphere Bacteria Help Tomato Plants Cope with Combined Phosphorus and Salt Stresses

2020 ◽  
Vol 8 (11) ◽  
pp. 1844
Author(s):  
Gylaine Vanissa Tchuisseu Tchakounté ◽  
Beatrice Berger ◽  
Sascha Patz ◽  
Matthias Becker ◽  
Henri Fankem ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Tomato Plant ◽  
Stress Conditions ◽  
Soil Conditions ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Salt Affected Soils ◽  
Phosphate Solubilizing

Plants are often challenged by multiple abiotic stresses simultaneously. The inoculation of beneficial bacteria is known to enhance plant growth under these stresses, such as phosphorus starvation or salt stress. Here, for the first time, we assessed the efficiency of selected beneficial bacterial strains in improving tomato plant growth to better cope with double stresses in salty and P-deficient soil conditions. Six strains of Arthrobacter and Bacillus with different reservoirs of plant growth-promoting traits were tested in vitro for their abilities to tolerate 2–16% (w/v) NaCl concentrations, and shown to retain their motility and phosphate-solubilizing capacity under salt stress conditions. Whether these selected bacteria promote tomato plant growth under combined P and salt stresses was investigated in greenhouse experiments. Bacterial isolates from Cameroonian soils mobilized P from different phosphate sources in shaking culture under both non-saline and saline conditions. They also enhanced plant growth in P-deficient and salt-affected soils by 47–115%, and their PGP effect was even increased in higher salt stress conditions. The results provide valuable information for prospective production of effective bio-fertilizers based on the combined application of local rock phosphate and halotolerant phosphate-solubilizing bacteria. This constitutes a promising strategy to improve plant growth in P-deficient and salt-affected soils.

scholarly journals Isolation, Characterization, and Molecular Identification of Phosphate Solubilizing Bacteria from Several Tropical Soils

2013 ◽  
Vol 18 (1) ◽  
pp. 67
Author(s):  
Fahrizal Hazra ◽  
Etty Pratiwi
Keyword(s):  
Acetic Acid ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Indole Acetic Acid ◽  
Tropical Soils ◽  
Rrna Gene ◽  
Gram Negative Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Gram Negative ◽  
Phosphate Solubilizing

The objectives of the research were: (i)  to isolate and characterize of phosphate solubilizing bacteria (PSB) and (ii) to identify PSB based on molecular amplification of 16S rRNA gene.  Soil samples were collected from rhizosphere in Bogor, West Nusa Tenggara, and East Nusa Tenggara.  Several stages in this research were: (i) isolation PSB in Pikovskaya agar, (ii) morphological and biochemical characterization of PSB, (iii) measurement of  phosphatase enzymes, and (iv) measurement of secreting indole acetic acid phytohormone.   As many as 29 isolates of PSB have been collected and three isolates of them, namely: P 3.5 (East Nusa Tenggara), P 6.2 (West Nusa Tenggara), and P 10.1 (Citeureup, West Java) were chosen for further study.  There were many characteristics of isolate P 10.1: (i) it had capable to solubilize P with the value of highest solubilization index (1.80), (ii) it had the highest phosphatase enzyme (120.40 mg kg-1), and (iii) it had the highest pH decrease at each observation for six days.  Isolates P 3.5 and P 10.1 were the Gram-negative bacteria with coccus shapes and isolate P 6.2 was a Gram-negative bacteria with bacillus shape.  Deoxiribonucleat Acid (DNA) amplification of these bacteria employing 16S rRNA primers generated the 1,300bp-PCR product.  The results of the analysis of 16S rRNA gene sequences showed that isolates P 3.5 and P 10.1 has 98% similarity with Gluconacetobacter sp. strains Rg1-MS-CO and isolate P 6.2 has 97% similarity with Enterobacter sp. pp9c strains.Keywords: 16S rRNA, indole acetic acid, isolation, phosphatase enzymes, phosphate solubilizing bacteria[How to Cite : Hazra F and E Pratiwi. 2013. Isolation, Characterization, and Molecular Identification of Phosphate Solubilizing Bacteria from Several Tropical Soils. J Trop Soils, 18 (1): 67-74. doi: 10.5400/jts.2013.18.1.67][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.67]

scholarly journals Screening, Identification and Growth Promotion Ability of Phosphate Solubilizing Bacteria from Soybean Rhizosphere under Maize-Soybean Intercropping Systems

2020 ◽  
Author(s):  
Wenjing Wang ◽  
Clement Kyei Sarpong ◽  
Chun Song ◽  
Xiaofeng Zhang ◽  
Yuefeng Gan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Agricultural Development ◽  
Rhizosphere Soil ◽  
Growth Promotion ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Aryabhattai ◽  
Promote Plant Growth ◽  
Phosphate Solubilizing

ABSTRACTThe solubilization and mineralization of phosphorus by phosphate-solubilizing bacteria (PSB) is one of the most important bacterial physiological characteristics in the soil biogeochemical cycle. Through the isolation and screening of microorganisms in the rhizosphere soil of intercropped soybean in Ya’an, Renshou and Chongzhou, 9 PSBs with high phosphorus solubilizing ability were identified. It mainly belongs to Bacillus and Pseudomonas. The phosphate solubility of Bacillus aryabhattai B8W22 is as high as 388.62 µg·mL-1. The physiological and biochemical characteristics of each strain showed that it can secrete organic acids such as formic acid, acetic acid lactic acid and pyruvic acid. In addition, all strains can produce indole acetic acid and siderophores that promote plant growth. Seed germination experiments also showed that the phosphorus solubilizing bacteria isolated in this research have a certain ability to promote plant growth.IMPRTANCEBacillus aryabhattai from rhizosphere soil of intercropped soybean has high phosphate-solubilizing ability, could produce indole acetic acid and siderophores that promote plant growth, and are of great significance in reducing the application of chemical phosphate fertilizers and promoting sustainable agricultural development.

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

Specificity of Phospholipid Binding to Indole Acetic Acid and Other Auxins

1974 ◽  
Vol 29 (1-2) ◽  
pp. 39-41 ◽  
Author(s):  
H. Veen
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phospholipid Binding ◽  
Primary Action

Abstract Studies in vitro on the binding of phospholipids with IAA, α-NAA and some of their biologically inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth.

Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

2021 ◽  
Vol 16 (8) ◽  
pp. 110-117
Author(s):  
Kannan Abhirami ◽  
K. Jayakumar
Keyword(s):  
Bacterial Isolate ◽  
Phosphate Solubilizing Bacteria ◽  
Cymbopogon Citratus ◽  
16S Rrna Sequence ◽  
Isolation And Characterization ◽  
16S Rrna Sequence Analysis ◽  
Phosphate Solubilizing ◽  
Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

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