scholarly journals Oxidative Stress Induced By The Metallic Oxide The Copper Oxide (Cuo-Nps) On Terrestrial Snail Helix Aspersa

2021 ◽  
Author(s):  
Benamara Maroua ◽  
Amrani Amina ◽  
Zaidi Hadjer ◽  
Sedrati Fateh ◽  
Bouroumana Selma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Copper Oxide ◽  
Land Snail ◽  
Copper Oxide Nanoparticles ◽  
Helix Aspersa ◽  
Oxidative Stress Biomarkers ◽  
Metallic Oxide ◽  
Cuo Nps ◽  
Metabolizing Enzyme ◽  
Snail Helix Aspersa

Abstract Our study focused on the evaluation of the toxicity of copper oxide nanoparticles (CuO-NPs) on a bioindicator; the land snail Helix aspersa. Their effects were studied by a targeted approach in the laboratory, by evaluating the oxidative stress biomarkers in hepatopancreas and kidney (GSH, GST, GPx, CAT, and LPO). The snails were exposed to increasing concentrations (50, 100, 150, and 200 mg/kg) of CuO-NPs mixed in wheat flour during a sub-chronic treatment period of 45 days. Our results show that: CuO-NPs can induce oxidative stress, by producing reactive oxygen species (ROS), which was confirmed by the decrease in glutathione (GSH) level and reduction of its metabolizing enzyme glutathione-s-transferase (GST) in both organs, as they trigger the detoxification system resulting in increased activity of the glutathione peroxidase (GPx) and catalase defense enzyme and lipide peroxidation indices within the hepatopancreas.

Cytotoxicity of Copper Oxide Nanoparticles in Human Alveolar Epithelial Cells

2011 ◽  
Vol 356-360 ◽  
pp. 2274-2277 ◽  
Author(s):  
Na Li ◽  
Hai Jun Sui ◽  
Dong Mei Gao
Keyword(s):  
Oxidative Stress ◽  
Copper Oxide ◽  
Membrane Damage ◽  
Copper Oxide Nanoparticles ◽  
Alveolar Epithelial Cells ◽  
Epithelial Cell Line ◽  
Oxide Nanoparticles ◽  
Alveolar Epithelial ◽  
Cuo Nps ◽  
Contraceptive Devices

Copper oxide nanoparticles (CuO NPs) are used in antimicrobial preparations, heat transfer fluids, semi-conductors or intrauterine contraceptive devices. Nanoparticles with unique magnetic, luminescent, and catalytic properties are being engineered for numerous biomedical applications, including imaging, diagnostics and therapy. However, potentially harmful interactions can occur between nanoparticles and living systems, including humans. The present study was aimed to assess the cytotoxicity of CuO NPs in human alveolar epithelial cell line (A549). A reduction in cell viability as a function of NP concentration for 24h was observed. CuO NPs were also found to induce oxidative stress in cells indicated by generation of H2O2. Our data demonstrates that CuO NPs even at low concentrations lead to cell membrane damage in human epidermal cells which may be mediated through lipid peroxidation and oxidative stress, however, induced little effect to Mitochondria. Hence, caution should be taken in their widely use as well as while handling.

Oxidative stress on land snail Helix aspersa as a sentinel organism for ecotoxicological effects of urban pollution with heavy metals

Chemosphere ◽  
2013 ◽  
Vol 93 (6) ◽  
pp. 1131-1138 ◽  
Author(s):  
K.Y. Abdel-Halim ◽  
A.M. Abo El-Saad ◽  
M.M. Talha ◽  
A.A. Hussein ◽  
N.M. Bakry
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Urban Pollution ◽  
Land Snail ◽  
Helix Aspersa ◽  
Ecotoxicological Effects ◽  
Snail Helix Aspersa

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

scholarly journals Green copper oxide nanoparticles for lead, nickel, and cadmium removal from contaminated water

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alaa El Din Mahmoud ◽  
Khairia M. Al-Qahtani ◽  
Sahab O. Alflaij ◽  
Salma F. Al-Qahtani ◽  
Faten A. Alsamhan
Keyword(s):  
Experimental Data ◽  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Bet Surface Area ◽  
Oxide Nanoparticles ◽  
Cadmium Removal ◽  
Cuo Nps ◽  
Pseudo Second Order ◽  
Isotherm And Kinetic Models ◽  
Lead Nickel

AbstractEnvironmentally friendly copper oxide nanoparticles (CuO NPs) were prepared with a green synthesis route without using hazardous chemicals. Hence, the extracts of mint leaves and orange peels were utilized as reducing agents to synthesize CuO NPs-1 and CuO NPs-2, respectively. The synthesized CuO NPs nanoparticles were characterized using scanning electron microscopy (SEM), Energy Dispersive X-ray Analysis (EDX), BET surface area, Ultraviolet–Visible spectroscopy (UV–Vis), and Fourier Transform Infrared Spectroscopy (FT-IR). Various parameters of batch experiments were considered for the removal of Pb(II), Ni(II), and Cd(II) using the CuO NPs such as nanosorbent dose, contact time, pH, and initial metal concentration. The maximum uptake capacity (qm) of both CuO NPs-1 and CuO NPs-2 followed the order of Pb(II) > Ni(II) > Cd(II). The optimum qm of CuO NPs were 88.80, 54.90, and 15.60 mg g−1 for Pb(II), Ni(II), and Cd(II), respectively and occurred at sorbent dose of 0.33 g L−1 and pH of 6. Furthermore, isotherm and kinetic models were applied to fit the experimental data. Freundlich models (R2 > 0.97) and pseudo-second-order model (R2 > 0.96) were fitted well to the experimental data and the equilibrium of metal adsorption occurred within 60 min.

scholarly journals Green Synthesis of Copper Oxide Nanoparticles Using Protein Fractions from an Aqueous Extract of Brown Algae Macrocystis pyrifera

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Karla Araya-Castro ◽  
Tzu-Chiao Chao ◽  
Benjamín Durán-Vinet ◽  
Carla Cisternas ◽  
Gustavo Ciudad ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Copper Oxide ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Brown Algae ◽  
Copper Oxide Nanoparticles ◽  
Macrocystis Pyrifera ◽  
Protein Fractions ◽  
Oxide Nanoparticles ◽  
Cuo Nps

Amongst different living organisms studied as potential candidates for the green synthesis of copper nanoparticles, algal biomass is presented as a novel and easy-to-handle method. However, the role of specific biomolecules and their contribution as reductant and capping agents has not yet been described. This contribution reports a green synthesis method to obtain copper oxide nanoparticles (CuO-NPs) using separated protein fractions from an aqueous extract of brown algae Macrocystis pyrifera through size exclusion chromatography (HPLC-SEC). Proteins were detected by a UV/VIS diode array, time-based fraction collection was carried out, and each collected fraction was used to evaluate the synthesis of CuO-NPs. The characterization of CuO-NPs was evaluated by Dynamic Light Scattering (DLS), Z-potential, Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM) equipped with Energy Dispersive X-ray Spectroscopy (EDS) detector. Low Molecular Weight (LMW) and High Molecular Weight (HMW) protein fractions were able to synthesize spherical CuO-NPs. TEM images showed that the metallic core present in the observed samples ranged from 2 to 50 nm in diameter, with spherical nanostructures present in all containing protein samples. FTIR measurements showed functional groups from proteins having a pivotal role in the reduction and stabilization of the nanoparticles. The highly negative zeta potential average values from obtained nanoparticles suggest high stability, expanding the range of possible applications. This facile and novel protein-assisted method for the green synthesis of CuO-NPs may also provide a suitable tool to synthesize other nanoparticles that have different application areas.

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