High Level of Mir-142-3P Expression in Peripheral Blood of Patients with Ovarian Carcinoma

Abstract Background The most common cancers detected in women are breast, thyroid, colorectal, uterine corpus, lung, and ovarian cancer. Ovarian cancer is responsible from more than 150.000 death annually worldwide. This cancer is detected in the late stage, and is characterised with poor prognosis, therefore most cases result with death. The fact that this cancer manifests itself in the late stage and is characterized by a poor prognosis, is caused death in the majority of cases. Therefore, the diagnosis and the treatment of the disease have to be improved for a better quality of life for patients. MicroRNAs are the noncoding RNAs in the length of 19–24 nucleotides which show suppressor effect on target genes. miRNAs are included in the pathology of various diseases including cancer. miRNAs being as the biomarker candidates in diagnosis, and their use in treatment as the inhibitors of the molecules mimicking the miRNA showed that they may be used as the new therapeutic target and agents. Methods We detected with our group in our prior study conducted with disconcordant ovarian cancer twins that many miRNA molecules were different in ovarian cancer compared with the molecules in healthy sibling. The expression level of miR-142-3p that was selected from the miRNAs detected in the previous study was compared, and investigated in a wider ovarian cancer group, and in healthy control group. miR-142-3p expression level was investigated using the real-time PCR method in the present study involving 147 patients, and 100 healthy control group. The differences in the expression levels of miR-142-3p detected in the peripheral blood lymphocytes of ovarian cancer patients, and healthy control were statisticaly evaluated. Results The expression level of miR-142-3p was detected to have increased 3.11 fold in ovarian cancer patients compared with the levels in healthy controls, and the difference was statistically significant (p:0.00). These results suggest that miR-142-3p that was found significantly increased in the peripheral blood samples of ovarian cancer patients compared with the healthy controls might be used as a sensitive, noninvasive biomarker in the early diagnosis, and treatment and follow up of ovarian cancer.

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The Aberrant Expression Levels of miR-423-5p and miR-664b-5p in Peripheral Blood of Patients With Familial and Sporadic Ovarian Carcinoma

10.21203/rs.3.rs-84141/v1 ◽

2020 ◽

Author(s):

BUSRA KURT ◽

Seref Bugra TUNCER ◽

Demet AKDENIZ ODEMIS ◽

Arash ADAMNEJAD GHAFOUR ◽

Mukaddes Avsar Sarali ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Patient ◽

Cancer Patients ◽

Peripheral Blood ◽

Ovarian Cancer Patient ◽

Expression Level ◽

Control Group ◽

Second Primary ◽

Aberrant Expression ◽

Significant Difference

Abstract MicroRNAs (miRNAs), are endogenous noncoding single strand RNA molecules with approximate length of 22 nucleotides. Ovarian cancer is a heterogenous disease which includes different biologic behaviors in the clinical, and molecular level. miRNAs have roles in the early diagnosis, prognosis, and chemotherapy sensitivity for ovarian cancer, and in the regulation of reproduction by being expressed in the ovaries. The expression level of two miRNAs of miR-423-5p, and miR-664b-5p selected from the miRNAs which were detected to be important for the ovarian cancer etiology in our previous studies were investigated in the peripheral blood of familial and sporadic ovarian cancer patients, and in healthy individuals. The expression analyses were statistically evaluated with SPSS v21.0 program using the Quantitative Real-Time PCR technique. A statistically significant difference was detected for both miRNAs between the healthy controls and ovarian cancer patient groups (p:0.000). The miR-423-5p expression was detected to increase 2.35 fold, and miR-664b-5p was detected to increase 2.47 fold in ovarian cancer patients compared with the levels in the control group. miR-423-5p was reported to be overexpressed in various cancer types, and in different disease stages in previous study, and miR-423-5p was demonstrated to have increased in the lymphocytes in the peripheral circulation of ovarian cancer patients first in our literature. The miR-664b-5p expression level was detected in high levels in ovarian cancer patient group, however was detected to have decreased in 64% of ovarian cancer patients with BRCA mutation carrier. The expressions of both miRNA molecules were detected to have decreased in the ovarian cancer subgroups in patients with ovarian cancer with addition of a second primary cancer of endometrium cancer, however, were detected in the highest level in ovarian cancer patients in addition to another secondary cancer except breast and endometrium cancer. The results obtained from the study showed that the increased expression of both miRNA molecules were suggested to be associated with ovarian cancer, and decrease was suggested to be associated with endometrium cancer. In summary, the miRNA molecules in the present study might be the non-invasive biological indicator in ovarian cancer, and the demonstration of these molecules on the tumor tissue, and their change during treatment in future studies will be beneficial.

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High Expression Level of miR-1260 Family in the Peripheral Blood of Patients with Ovarian Carcinoma

10.21203/rs.3.rs-79413/v1 ◽

2020 ◽

Author(s):

Arash Adamnejad Ghafour ◽

Demet Akdeniz Odemis ◽

Seref Bugra Tuncer ◽

Busra Kurt ◽

Mukaddes Avsar Sarali ◽

...

Keyword(s):

Ovarian Cancer ◽

Cervical Cancer ◽

Cancer Treatment ◽

Cancer Patients ◽

Peripheral Blood ◽

Uterine Cancer ◽

Expression Level ◽

Control Group ◽

Gynecologic Cancers ◽

Expression Levels

Abstract The most common gynecologic cancers detected in women in Turkey are uterine cancer, ovarian cancer, and cervical cancer. These data reported that a mean of 3800 individuals were diagnosed with uterine cancer, 2790 were diagnosed with ovarian cancer, and 1950 were diagnosed with cervical cancer, and 400 individuals were diagnosed with other gynecologic cancers each year in Turkey. A mean of 14.270 individuals were detected to have been diagnosed with gynecologic cancers each year in the United States of America(USA). Ovarian cancer treatment is generally composed of chemotherapy, and surgery. In general, chemotherapy is administered after surgery. The identification of the molecular pathogenesis of ovarian cancer, and discovery of new moleculer biomarkers which facilitate the ovarian cancer treatment are required for an effective ovarian cancer treatment in clinics. miRNAs are reported to be the possible biologic indicators for various cancer types. We aimed to investigate 2 miRNAs which were suggested to have effect in ovarian cancer in our (previous)monozygotic twin study from miR-1260 microRNA family whose association with ovarian cancer yet has not been reported in the literature. We investigated the expression levels of miR-1260a, and miR-1260b miRNAs, in the peripheral blood lymphocytes of 150 familial and sporadic ovarian cancer patients, and of 100 healthy individuals of the control group who were matched for age, sex, and ethnicity with the patient group, and investigated their possible property of being a biologic indicator for ovarian cancer. The expression results of ovarian cancer patients were evaluated by comparison of the results of the control group in the study. The expression levels of miR-1260a, and miR-1260b in ovarian cancer patients were found highly increased compared with the levels in the control group. miR-1260a expression level in ovarian cancer patients was detected to have increased approximately 17 fold compared with the control group, and miR-1260b expression level in ovarian cancer patients was detected to have increased approximately 33 fold compared with the levels in the control group. The String Analyses showed that the miR-1260a was associated with the ribosomal protein family which was known to be effective in the translation stage of cell and that miR-1260b was associated with CHEK2 protein which was a member of the serine/threonine-protein kinase family. It should be investigated for larger cohorts in benign ovarian diseases and in different stages of patients receiving ovarian cancer treatment whether these two molecules are a noninvasive biomarker and therapeutic target to be used especially in the early diagnosis and prognosis of ovarian cancer in future.

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High expression level of miR-1260 family in the peripheral blood of patients with ovarian carcinoma

Journal of Ovarian Research ◽

10.1186/s13048-021-00878-x ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Arash Adamnejad Ghafour ◽

Demet Akdeniz Odemis ◽

Seref Bugra Tuncer ◽

Busra Kurt ◽

Mukaddes Avsar Saral ◽

...

Keyword(s):

Ovarian Cancer ◽

Cervical Cancer ◽

Cancer Treatment ◽

Cancer Patients ◽

Peripheral Blood ◽

Uterine Cancer ◽

Expression Level ◽

Control Group ◽

Gynecologic Cancers ◽

Expression Levels

AbstractThe most common gynecologic cancers detected in women in Turkey are uterine cancer, ovarian cancer, and cervical cancer. These data reported that a mean of 3800 individuals were diagnosed with uterine cancer, 2790 were diagnosed with ovarian cancer, and 1950 were diagnosed with cervical cancer, and 400 individuals were diagnosed with other gynecologic cancers each year in Turkey. A mean of 14.270 individuals were detected to have been diagnosed with gynecologic cancers each year in the United States of America (USA). Ovarian cancer treatment is generally composed of chemotherapy, and surgery. In general, chemotherapy is administered after surgery. The identification of the molecular pathogenesis of ovarian cancer, and discovery of new moleculer biomarkers which facilitate the ovarian cancer treatment are required for an effective ovarian cancer treatment in clinics. miRNAs are reported to be the possible biologic indicators for various cancer types. We aimed to investigate 2 miRNAs which were suggested to have effect in ovarian cancer in our (previous) monozygotic twin study from miR-1260 microRNA family whose association with ovarian cancer yet has not been reported in the literature. We investigated the expression levels of miR-1260a, and miR-1260b miRNAs, in the peripheral blood lymphocytes of 150 familial and sporadic ovarian cancer patients, and of 100 healthy individuals of the control group who were matched for age, sex, and ethnicity with the patient group, and investigated their possible property of being a biologic indicator for ovarian cancer. The expression results of ovarian cancer patients were evaluated by comparison of the results of the control group in the study. The expression levels of miR-1260a, and miR-1260b in ovarian cancer patients were found highly increased compared with the levels in the control group. miR-1260a expression level in ovarian cancer patients was detected to have increased approximately 17 fold compared with the control group, and miR-1260b expression level in ovarian cancer patients was detected to have increased approximately 33 fold compared with the levels in the control group. The String Analyses showed that the miR-1260a was associated with the ribosomal protein family which was known to be effective in the translation stage of cell and that miR-1260b was associated with CHEK2 protein which was a member of the serine/threonine-protein kinase family. It should be investigated for larger cohorts in benign ovarian diseases and in different stages of patients receiving ovarian cancer treatment whether these two molecules are a noninvasive biomarker and therapeutic target to be used especially in the early diagnosis and prognosis of ovarian cancer in future.

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MicroRNA-133a-5p Promotes Angiogenesis of Endothelial Progenitor Cells via TRIM59/Id1 in Ovarian Cancer

10.21203/rs.3.rs-34808/v1 ◽

2020 ◽

Author(s):

Lichen Teng ◽

Jian Wu ◽

Huiling Li ◽

Huiyan Wang ◽

Ying Wang ◽

...

Keyword(s):

Ovarian Cancer ◽

Progenitor Cells ◽

Cancer Patients ◽

Endothelial Progenitor Cells ◽

Target Protein ◽

Expression Level ◽

Vegf Expression ◽

Endothelial Progenitor ◽

Control Subjects ◽

Healthy Control

Abstract Background Endothelial progenitor cells (EPCs) play an important role in tumor angiogenesis and growth. In this study, we assessed the effect of MicroRNAs (miRNAs) on EPCs migration and angiogenesis and its signaling pathway in patients suffered from ovarian cancer (OC). Methods We cultured peripheral circulating EPCs derived from 32 OC patients and 20 healthy control subjects, respectively. The miRNA profiles of EPCs in ovarian cancer patients were compared with that in healthy control subjects, and aberrantly expressed miRNAs in both groups were identified via miRNA microarray and clustering analysis. Among these miRNAs, miR-133a-5p was considered as one of the most important miRNAs, which biological function in EPCs has been investigated. Bioinformatic analysis combined with knockdown and overexpression of miR-133a-5p were used to identify its target protein. Results An obviously downregulated expression level of miR-133a-5p has been seen in EPCs with ovarian cancer patients. Downregulated expression level of miR-133a-5p has been seen in ovarian cancer tissues and ovarian cancer cells (SKOV-3 and OVCAR-3). Downregulated of miR-133a-5p can increase TRIM59 expression, moreover, downregulated of miR-133a-5p further induce migration and angiogenesis via increase VEGF and Id1 in EPCs. MiR-133a-5p pro-angiogenesis would be diminished by TRIM59 knockdown. Conclusions The study found that miR-133a-5p was an important upstream factor regulated Id1/VEGF expression. Additionally, functional studies have revealed that TRIM59 was a direct target protein of miR-133a-5p, and TRIM59 silencing attenuated the role of miR-133a-5p in angiogenesis and Id1/VEGF expression. So we proposed that miR-133a-5p would be a new target for OC therapy.

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Th Cytokine Profile in Childhood-Onset Systemic Lupus Erythematosus

10.21203/rs.3.rs-130849/v1 ◽

2021 ◽

Author(s):

Wei Quan ◽

Jingnan An ◽

Gang Li ◽

Guanghui Qian ◽

Meifang Jin ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Disease Activity ◽

Lupus Erythematosus ◽

Peripheral Blood ◽

Healthy Control Group ◽

Control Group ◽

Healthy Children ◽

Childhood Onset ◽

Systemic Lupus ◽

Healthy Control

Abstract Background: Childhood-onset systemic lupus erythematosus (cSLE) is a kind of chronic inflammatory disease characterized by a highly abnormal immune system. This study aimed to detect expression of the Th cytokines IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17A, IL-17F, IL-21, IL-22, IFN-γ and TNF-α in the peripheral blood of children with cSLE; clinical symptoms; and a disease index and discuss the relationship between the Th cell cytokine regulatory network and onset of systemic lupus erythematosus (SLE) in children and disease outcome.Methods: A total of 33 children with cSLE and 30 healthy children were enrolled in this study. Children in the cSLE group were classified into the inactive cSLE group or active cSLE group according to their SLE disease activity index 2000 (SLEDAI-2K). Th cytokine profiles in peripheral blood of different groups were detected and analyzed.Results: The levels of IL-2, IL-10 and IL-21 in the cSLE group were significantly higher than those in the healthy control group (P < 0.05, P<0.01 and P<0.01, respectively). The expression of IL-2, IL-10 and IL-21 in the active cSLE group was significantly higher than that in the healthy control group (P<0.05, P<0.01 and P<0.05, respectively), but IL-22 expression was remarkably lower in the active cSLE group than in the healthy control group (P<0.001). IL-21 in the inactive SLE group was significantly higher than that in the healthy control group (P<0.05). The levels of IL-2 and IL-10 in the active cSLE group were significantly higher than those in the inactive cSLE group (P<0.01 and P<0.05). In-depth analysis showed that the expression levels of IL-2 (r=0.382, P=0.028), IL-6 (r=0.514, P=0.002) and IL-10 (r=0.429, P=0.016) were positively correlated with disease activity. Conclusion: This study provides a theoretical basis for the discovery of effective methods to regulate imbalance in T lymphocyte subsets in cSLE, which may open up potential new approaches for the diagnosis of cSLE.

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MicroRNA-133a-5p Promotes Angiogenesis of Endothelial Progenitor Cells via TRIM59/Id1 in Ovarian Cancer

10.21203/rs.3.rs-52314/v1 ◽

2020 ◽

Author(s):

Lichen Teng ◽

Jian Wu ◽

Huiling Li ◽

Huiyan Wang ◽

Ying Wang ◽

...

Keyword(s):

Ovarian Cancer ◽

Progenitor Cells ◽

Cancer Patients ◽

Endothelial Progenitor Cells ◽

Target Protein ◽

Expression Level ◽

Vegf Expression ◽

Endothelial Progenitor ◽

Control Subjects ◽

Healthy Control

Abstract Background: Endothelial progenitor cells (EPCs) play an important role in tumor angiogenesis and growth. Our previous study has indicated that over-expressed inhibitor of DNA binding/differentiation 1 (Id1) in EPCs could promote EPCs proliferation, migration, and adhesion. In this study, we assessed the effect of MicroRNAs (miRNAs) on EPCs migration and angiogenesis and its signaling pathway in patients suffered from ovarian cancer (OC).Methods: We cultured peripheral circulating EPCs derived from 32 OC patients and 20 healthy control subjects, respectively. The miRNA profiles of EPCs in ovarian cancer patients were compared with that in healthy control subjects, and aberrantly expressed miRNAs in both groups were identified via miRNA microarray and clustering analysis. Among these miRNAs, miR-133a-5p was considered as one of the most important miRNAs, which biological function in EPCs has been investigated. Bioinformatic analysis combined with knockdown and overexpression of miR-133a-5p were used to identify its target protein.Results: An obviously downregulated expression level of miR-133a-5p has been seen in EPCs with ovarian cancer patients. Downregulated expression level of miR-133a-5p has been seen in ovarian cancer tissues and ovarian cancer cells (SKOV-3 and OVCAR-3). Downregulated of miR-133a-5p can increase TRIM59 expression, moreover, downregulated of miR-133a-5p further induce migration and angiogenesis via increase VEGF and Id1 in EPCs. MiR-133a-5p pro-angiogenesis would be diminished by TRIM59 knockdown. Additionally, increased TRIM59 also can promote EPCs migration and angiogenesis.Conclusions: The study found that miR-133a-5p was an important upstream factor regulated Id1/VEGF expression. Additionally, functional studies have revealed that TRIM59 was a direct target protein of miR-133a-5p, and TRIM59 silencing attenuated the role of miR-133a-5p in angiogenesis and Id1/VEGF expression. So we proposed that miR-133a-5p would be a new target for OC therapy.

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Important serum markers in malignant lymphoma

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.e22225 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. e22225-e22225

Author(s):

S. Yavuz ◽

M. Erkisi ◽

I. Petekkaya ◽

N. Basel

Keyword(s):

Malignant Lymphoma ◽

Peripheral Blood ◽

Serum Markers ◽

University Hospital ◽

Healthy Control Group ◽

Control Group ◽

Host Immune System ◽

Acute Phase Reactants ◽

Healthy Control ◽

Patient Groups

e22225 Background: In this study, 78 patients with new diagnosed, 21 patients with relapsed malignant lymphoma who applied to Cukurova University Hospital between March 2006 - 2008, and 36 age and sex matched healthy control group were evaluated and have been followed up. Methods: The aim of this study was to investigate if; any acute phase reactants or lymphocyte markers in peripheral blood have any predictive role concerning the treatment response, or disease progression. Results: Peripheral blood CD20 (+) lymphocyte levels were slightly higher in new diagnosed patients (12.09±13.79), than the control group (11.25 ±4. 79) but, much lower in relapsed patients (7.30±9.51, P= 0.038). After the chemotherapy (CT), CD20 (+) cell percentage decreased significantly only in new diagnosed patients (p<0.001). Pretreatment CD20 (+) cell levels were higher in responding patients than no responders (15. 42 ± 13.30 versus, 6.72 ± 5.24 p= 0.052). Peripheral blood CD 4 (+) cell levels were below the healthy control group (p= 0.01) and remained low after the CT. Interestingly, CD8 (+) cell levels increased in responders, after the CT (p= 0.046) in both patient groups. CD 56(+) lymphocyte levels were higher only in new diagnosed patients than healthy group (p= 0.05). Its level increased further after the CT (p= 0.044). Serum TNF α levels were higher in patient groups than control (p<0.001). Its level decreased following CT (p= 0.002). CRP levels were higher in both patient groups and remained high following the CT (p<0.001), regardless of the response status. Ferritin levels were also higher in patients groups (p<0.001). Pre-treatment serum ferritin levels were lower in responders, than no responders (236.65 ± 242.17 ng/ml versus 718.77 ± 645.24 ng/ml, p= 0.02). Serum prealbumine levels were lower in lymphoma patients than the healthy controls (p< 0.001). Its level was increased after treatment, especially in patients with recurrent disease (21.15± 5.89 versus 26.60 ± 7.29 mg/dl, p= 0.019). Conclusions: In conclusion, it was decided that; during the different stages of lymphoma progression, several mutations may occur, in the different components of the host immune system. Some of the immune responses would continue in spite of complete clinical remission, as some others would predict the response. No significant financial relationships to disclose.

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AB0043 THE IMBALANCE OF T FOLLICULAR REGULATORY CELL AND T FOLLICULAR HELPER CELL IN RHEUMATOID ARTHRITIS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.1311 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 1324-1325

Author(s):

R. Su ◽

Y. Y. Wang ◽

F. Y. Hu ◽

X. Zheng ◽

Y. Liu ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Peripheral Blood ◽

Control Group ◽

Healthy Controls ◽

Regulatory Cells ◽

Regulatory Cell ◽

Helper Cells ◽

Follicular Helper ◽

Healthy Control ◽

T Follicular Helper Cell

Background:Rheumatoid arthritis (RA) is a chronic inflammatory disease which can lead to severe joint damage and disability.The relationship between antibodies and rheumatoid arthritis has long been well established. Recently, many studies have found that T follicular regulatory cells(Tfr) and T follicular helper cells (Tfh) are closely related to antibody generation on lymphoid follicular germinal centers (GCs)[1-2]. Tfr cells can inhibite the GC reaction and suppress production of high-affinity antibodies. The dysregulation of Tfh cells can lead to the production of autoantibodies by B cells.Objectives:To examine the expression of circulating T follicular regulatory cell (Tfr) and T follicular helper cell and its subsets(Tfh1 Tfh2 Tfh17) in RA patients and healthy control group.Methods:Level of Tfr and Tfh1,Tfh2 and Tfh17 cells in the peripheral blood of 17 new RA patients, 30 treated RA patients and 18 healthy controls were deceted by flow cytomery. All patients were hospitalised at the Department of Rheumatology, Second Hospital of Shanxi Medical University.Results:We found that the level of Tfr (CD3+CD4+CD25+CXCR5+FOP3+) percent(P=0.020), in the peripheral blood in RA patients were significantly decreased compared with healthy controls. The percent of Tfh (CD3+CD4+CXCR5+CD45RA-) (P=0.039)and Tfh17 (CD3+CD4+CXCR5+CD45RA-CXCR3-CCR6+) (P=0.000)were increased, but there are no statistical difference about Tfh1(CD3+CD4+CXCR5+CD45RA-CXCR3+CCR6-)(P=0.558) and Tfh2 (CD3+CD4+CXCR5+CD45RA-CXCR3-CCR6-) percent(P=0.079). We compared the above indicators between new and treated RA patients, and the results indicated that the Tfr(P=0.013),Tfh (P=0.002) and Tfh1(P=0.034) were significantly increased in the new RA patients compared to the treated RA patients, there were no differences between the two groups in Tfh2(P=0.419) and Tfh17 percent(P=0.124).Conclusion:Our results indicated that disorder of Tfr and Tfh subsets were involved in RA, restoring the Tfr/Tfh balance may be the potential therapeutic targets.Fig. 1.Comparison of Tfr, Tfh and its subsets(Tfh1 Tfh2 Tfh17) percent among the RA patients (n = 47) and healthy control group (n = 18) (*P < 0.05).Fig. 2.Comparison of Tfr,Tfh and its subsets(Tfh1 Tfh2 Tfh17) percent among the new RA patients (n = 17) and treated RA patients(n = 30) (*P < 0.05).References:[1]Deng J, Wei Y, Fonseca VR, Graca L, Yu D.T follicular helper cells and T follicular regulatory cells in rheumatic diseases[J].Nat Rev Rheumatol. 2019, 15(8):475-490.[2]Chen Liu, Dongwei Wang, Songsong Lu, et al.Increased Circulating Follicular Treg Cells Are Associated With Lower Levels of Autoantibodies in Patients With Rheumatoid Arthritis in Stable Remission.Arthritis Rheumatol. 2018, 70(5):711-721Disclosure of Interests:None declared

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Erythropoiesis differences in various clinical phases of Dengue fever using Immature Reticulocyte Fraction parameter

10.1101/563924 ◽

2019 ◽

Author(s):

Amaylia Oehadian ◽

Putri Vidyaniati ◽

Jeffery Malachi Candra ◽

Uun Sumardi ◽

Evan Susandi ◽

...

Keyword(s):

Dengue Fever ◽

Dengue Infection ◽

Healthy Control Group ◽

Control Group ◽

Baseline Characteristic ◽

Healthy Controls ◽

Research Subjects ◽

Critical Phase ◽

Phase Group ◽

Healthy Control

AbstractDengue fever is the most common cause of hospitalization in otherwise healthy person. The incidence rate increases significantly every year, from 0.05 per 100.000 inhabitants in 1968 to 35-40 per 100.000 inhabitants in 2013. Thrombocytopenia and leucopenia are the most commonly reported changes, whether they are caused by bone marrow suppression or peripheral destruction. Anemia has also been observed to occur in dengue infection with unknown mechanism. Immature Reticulocyte Fraction (IRF) is a parameter reflecting the most immature reticulocyte fraction and it can identify the earliest stage of erythropoiesis disorder. This research aimed to determine the mechanism of erythropoiesis disorders that led to anemia using IRF parameter in the various clinical phases of dengue fever. This study was a comparative analytical research using secondary data derived from the Dengue-associated Endothelial Cell Dysfunction and Thrombocyte Activation (DECENT) research. The baseline characteristic data consists of sex, age, level of hemoglobin, hematocrit, leucocyte, and thrombocyte, also the IRF. The patients were grouped into the fever, critical, recovery, and convalescent phases, plus healthy control group. The data was analyzed using the Kolmogorov-Smirnov normality test, followed by Friedman test and Mann-Whitney post hoc test. There were 244 research subjects and the median age was 24 (14-67), with similar ratio of male and female. The median IRF for all the research subjects was 4.8% with an IQR of 2.4-8.1%. The fever-phase group showed a median of 1.8% with an IQR of 0.5-2.85%. The critical-phase group showed a median of 3.6% with an IQR of 1.8- 5.0%, while the median for the recovery-phase group was 7.05% with an IQR of 4.08-11.85%. The convalescent-phase group showed a median of 7.3 % with an IQR of 3.95-9.3%, and the healthy-control group showed a median of 4.1% with an IQR of 2.2-6.6%. There was a significant difference in IRF between the groups (p<0.05). Immature Reticulocyte Fraction in fever phase was significantly different with IRF in other phases and healthy controls (p<0.05). In the critical phase, IRF was not significantly different from the healthy controls (p=0.218). Summarizing, there are changes in erythropoiesis activity in various clinical phases of dengue infection. Erythropoiesis suppression occurred mainly during the fever phase and it started to restore in the critical phase. In the recovery and convalescent phases, the erythropoiesis activity was increased.

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Different vitamin D status in common multiorgan autoimmune disease patients

Journal of Laboratory Medicine ◽

10.1515/labmed-2019-0104 ◽

2019 ◽

Vol 43 (5) ◽

pp. 243-247

Author(s):

Erfu Xie ◽

Zhongjian Zhao ◽

Chengjing Yan ◽

Yiting Zhang ◽

Qiaodi Zhang ◽

...

Keyword(s):

Vitamin D ◽

Lupus Erythematosus ◽

Pairwise Comparison ◽

Vitamin D Supplementation ◽

Healthy Control Group ◽

Group Differences ◽

Control Group ◽

Healthy Controls ◽

Vitamin D Status ◽

Healthy Control

Abstract Background Vitamin D plays a key role in calcium homeostasis and contributes to the regulation of the immune system. Furthermore, vitamin D deficiency has been reported to be associated with autoimmune diseases (AIDs), especially with multiorgan AIDs. Various multiorgan AIDs may be different based on the vitamin D status. This study aims to investigate the serum 25-hydroxyvitamin D (25(OH)D) levels in patients with different common multiorgan AIDs. Methods A total of 295 patients with multiorgan AIDs treated in our hospital from January 2012 to September 2018 were recruited, including 137 cases of rheumatoid arthritis (RA), 85 cases of systemic lupus erythematosus (SLE), 32 cases of Sjögren’s syndrome (SS) and 41 cases of mixed connective tissue disease (MCTD); 47 apparently healthy individuals were also recruited as controls. The serum 25(OH)D levels in patients with different multiorgan AIDs were measured with Roche electrochemiluminescence immunoassay and statistically analyzed the proportion of patients with normal, insufficiency and deficiency in 25(OH)D levels in different multiorgan diseases. The 25(OH)D levels of different multiorgan AID groups and healthy controls were also compared. Results Incidences of 25(OH)D deficiency in the RA, SLE, SS and MCTD groups were 21.2%, 35.3%, 25.0% and 22.0%, respectively, with significant inter-group differences (p < 0.05). The incidence in the SLE group was higher than in the RA, SS and MCTD groups, indicating severe 25(OH)D deficiency in patients with SLE. Significant inter-group differences (p < 0.05) were detected in the serum 25(OH)D levels in different multiorgan AID groups and in the healthy control group. Further pairwise comparison found a significantly higher level of 25(OH)D in the healthy control group than in the SLE, SS, RA and MCTD groups (p < 0.05). Moreover, the 25(OH)D status in the SLE group was significantly lower than that in the SLE, SS, RA and MCTD groups (p < 0.05). Conclusions Serum 25(OH)D deficiency and a low 25(OH)D status are commonly seen in patients with different multiorgan AIDs compared to healthy controls, warranting vitamin D supplementation. Severe 25(OH)D deficiency and a lower 25(OH)D status were found in patients with SLE.

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