scholarly journals Urine Specimen Collection For Diagnosing a Urinary Tract Infection-Patient Experience and Preference For Urine Specimen Collection: A Qualitative Study

Author(s):  
Linda Collins ◽  
Rajvinder Khasriya ◽  
James Malone-Lee

Abstract Background A urinary tract infection (UTI) is very common and patients frequently present to their General Practitioner and emergency department with symptoms. Diagnosis of the infection is made by examining fresh urine with microscopy, to detect white cells and epithelial cells shed from the bladder or by submitting the urine for microbial culture. Patients are often requested to provide a urine specimen, but very little is known about the patient experiences of carrying out the task or their preference of specimen collection method. The aim of this study was to explore patient experiences and preferences between four different urine specimen collection methods for diagnosing the presence of a UTI.Materials Semi-structured interviews were conducted and explored patient experiences of four different urine specimen collection methods (midstream urine specimen (MSU), catheter specimen (CSU), Peezy MSU™ and Natural void). The interviews consisted of four open-ended questions and all data were collated and organised using NVivo to establish themes that emerged from the data.Results The natural void without technique was repeatedly referred to and was regarded as being the most straightforward of all of the sampling methods. Pain symptoms were described only in the catheter method and not in the MSU, Peezy MSU™ or natural void method. The catheter was regarded as the urine collection method that produced the best specimen for diagnostic testing by the patients’. Conclusion A majority of the participants expressed the desire for straightforward urine sampling methods and preferred the natural void as a standard method in clinical practice.

2019 ◽  
Vol 6 ◽  
pp. 2333794X1882194
Author(s):  
Kaori Kamijo ◽  
Yoshifusa Abe ◽  
Takehi Kagami ◽  
Kazuhisa Ugajin ◽  
Takeshi Mikawa ◽  
...  

We report the case of a 2-month-old infant with incomplete Kawasaki disease that presented as an apparent urinary tract infection. The patient’s fever persisted despite antibiotic treatment. Intravenous immunoglobulin and aspirin therapy cured both the incomplete Kawasaki disease and bacterial pyuria. Renal sonography, voiding cystourethrography, and renal parenchyma radionuclide scanning did not detect any abnormalities. Temporary dilation of the coronary artery was noted. In a urine specimen obtained through transurethral catheterization, the growth of 105 colony-forming units/mL of extended-spectrum β-lactamase–producing Escherichia coli was detected. Polymerase chain reaction analysis revealed that the enzyme genotype was CTX-M-8, which is a rare type in Japan. In conclusion, attention should be paid to a misleading initial presentation of fever and pyuria, which might be interpreted as urinary tract infection in patients with Kawasaki disease. Furthermore, pediatricians should consider incomplete Kawasaki disease when patients present with fever and pyuria, which are consistent with urinary tract infection, but do not respond to antibiotic treatment.


2016 ◽  
Vol 216 ◽  
pp. 168-173 ◽  
Author(s):  
T.M. Sørensen ◽  
A.B. Jensen ◽  
P. Damborg ◽  
C.R. Bjørnvad ◽  
L. Guardabassi ◽  
...  

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Thiago G. S. Paim ◽  
Luiza Pieta ◽  
Janira Prichula ◽  
Gustavo E. Sambrano ◽  
Renata Soares ◽  
...  

We report here a draft genome sequence ofEnterococcus faecalisstrain F165 isolated from a urine specimen in South Brazil. The genome size was 3,049,734 bp, with a G+C content of 37.38%, and genes related to antimicrobial resistance and adherence were found in the strain. These findings are consistent with pathogenesis ofE. faecalisspecies.


2015 ◽  
Vol 104 (9) ◽  
pp. e395-e400 ◽  
Author(s):  
J Verliat-Guinaud ◽  
P Blanc ◽  
F Garnier ◽  
V Gajdos ◽  
V Guigonis

2015 ◽  
Vol 53 (4) ◽  
pp. 1282-1285 ◽  
Author(s):  
Joseph B. Cantey ◽  
Claudia Gaviria-Agudelo ◽  
Erin McElvania TeKippe ◽  
Christopher D. Doern

Urinary tract infection (UTI) is one of the most common infections in children. Urine culture remains the gold standard for diagnosis, but the utility of urine Gram stain relative to urinalysis (UA) is unclear. We reviewed 312 pediatric patients with suspected UTI who had urine culture, UA, and urine Gram stain performed from a single urine specimen. UA was considered positive if ≥10 leukocytes per oil immersion field were seen or if either nitrates or leukocyte esterase testing was positive. Urine Gram stain was considered positive if any organisms were seen. Sensitivity, specificity, and positive and negative predictive values were calculated using urine culture as the gold standard. Thirty-seven (12%) patients had a culture-proven UTI. Compared to urine Gram stain, UA had equal sensitivity (97.3% versus 97.5%) and higher specificity (85% versus 74%). Empirical therapy was prescribed before the Gram stain result was known in 40 (49%) patients and after in 42 (51%) patients. The antibiotics chosen did not differ between the two groups (P= 0.81), nor did they differ for patients with Gram-negative rods on urine Gram stain compared to those with Gram-positive cocci (P= 0.67). From these data, we conclude that UA has excellent negative predictive value that is not enhanced by urine Gram stain and that antibiotic selection did not vary based on the urine Gram stain result. In conclusion, the clinical utility of urine Gram stain does not warrant the time or cost it requires.


2015 ◽  
Vol 9 (2) ◽  
pp. 64-69
Author(s):  
Nishat Jubaida ◽  
Narjis Maliha Kawsar ◽  
Nahid Elora ◽  
Md Rahimgir ◽  
Nahid Reaz Shapla ◽  
...  

Introduction: Women with asymptomatic bacteriuria during pregnancy are more likely to deliver premature or low birth weight babies and having a 20 – 40 percent increased risk of developing pyelonephritis during pregnancy compared with women without bacteriuria. Asymptomatic bacteriuria is a microbiological diagnosis based on urine culture from persons without signs or symptoms of urinary tract infection. Objectives: The aim of this study was to determine the prevalence of asymptomatic bacteriuria in pregnant women and also to determine the relationship between the asymptomatic bacteriuria and pyuria to isolate the causative pathogens and to find out the antimicrobial susceptibility pattern of the isolates. Methods: A total of 540 urine specimen were collected from 540 asymptomatic pregnant women for urinary tract infection, over a period 2 years from January 2011 to December 2012 at Border Guard Bangladesh (BGB) Hospital, Pilkhana, Dhaka. All specimens were processed using standard microbiological procedures. All the subjects were evaluated for bacteriuria. Results: The prevalence of asymptomatic bacteriuria was 10.2% . Pyuria was found in 15.9% of cases. Escherichia coli (80%) was the commonest causative agent of asymptomatic bacteriuria followed by Staphylococcus saprophyticus (14.5%) and Pseudomonas spp.(5.5%). Nitrofurantoin was found to be highly sensitive to most of the isolates. 64 JAFMC Bangladesh. Vol 9, No 2 (December) 2013 Conclusion: Screening for bacteriuria in pregnancy and proper treatment of diagnosed cases must be considered as an essential part of antenatal care in our country. To prevent complications of asymptomatic bacteriuria, all pregnant women should be screened at the first antenatal visit. A negative test for pyuria is not a reliable indicator of the absence of asymptomatic bacteriuria in pregnant women. DOI: http://dx.doi.org/10.3329/jafmc.v9i2.21830 Journal of Armed Forces Medical College Bangladesh Vol.9(2) 2013


2002 ◽  
Vol 13 (12) ◽  
pp. 801-804 ◽  
Author(s):  
Andrew Leung ◽  
Shaw Taylor ◽  
Angela Smith ◽  
Robert Spencer ◽  
Paddy Horner

In young sexually active male patients, clinical differentiation between non-gonococcal urethritis (NGU) and urinary tract infection (UTI) can be difficult. UTI as a cause of NGU has been suggested before, but the prevalence of UTI among acute NGU patients has not been evaluated. We conducted a prospective cross-sectional study of 156 male patients with clinical features of acute urethritis. The prevalence of UTI among acute NGU patients was 6.4% (CI: 1.5–11.3%). There was a possible association between UTI and NGU but this was not statistically significant. The sensitivity and specificity for combining leucocyte esterase and nitrite tests were 83.3% and 89.8% respectively, with a negative predictive value of 98.8%. We conclude that a mid-stream urine specimen should routinely be collected in patients with symptoms of urethritis. This should be analysed by dipsticks incorporating nitrite and leucocyte esterase tests. Those with a positive dipstick should then be confirmed by culture.


Author(s):  
Bhavana Ashish Kakaria ◽  
Ashish K. ◽  
Raghuwanshi Tushar

Background: Catheter-associated urinary tract infections (CAUTIs) are the most common nosocomial infection and a leading cause of morbidity and mortality in hospitalized patients. The aim of this study was to determine the incidence and risk factors of urinary tract infection in catheterised patients in a tertiary care hospital.Methods: Total of 200 patients above 16 years in whom an indwelling Foley‘s catheter inserted were taken in the study. A urine specimen was obtained aseptically and culture done on nutrient agar, sheep blood agar and MacConkey agar plates. After incubation of 24 hours, colony count done for organisms showing growth and colony count ≥105 was taken significant.Results: The incidence of CAUTI in the present study was 31%. Higher incidence of CAUTI (56.46%) was found in female sex as compared to males (43.54%). Incidence of CAUTI was found higher in first weeks (54.83%). Incidence among diabetes patients is found more (63.33%). Most common isolate found was E. coli (38.71%) among all uropathogens. Uropathogens isolated from CAUTI are more resistant to antimicrobials.Conclusions: It is must to implement following strategy for reducing the risk of infection due to indwelling catheters: 1. reducing the duration of catheterization 2. Use antibacterial substance coated catheter 3. Strict infection control measures.


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