scholarly journals Sperm cryopreservation of male cancer patients for  fertility preservation : 10-year monocentric experience

2021 ◽  
Author(s):  
Xiao Liu ◽  
Bo Liu ◽  
shasha liu ◽  
yang xian ◽  
wenrui zhao ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cancer Patients ◽  
Fertility Preservation ◽  
Sperm Quality ◽  
Germ Cell Tumors ◽  
Semen Parameters ◽  
Sperm Cryopreservation ◽  
Sperm Banking ◽  
Hematological Neoplasms ◽  
Body Regions

Abstract Background: Semen cryopreservation is an effective method to preserve fertility, which is very important for male cancer patients. Unfortunately, due to unaware of the opportunities for sperm cryopreservation for both physicians and cancer patients, not a lot of data on evaluating the semen parameters and dispositions of the cryopreserved samples of Chinese cancer population are available in the literature. Methods: We retrospectively reviewed semen parameters as well as the clinical outcomes of assisted reproductive treatments (ART) of 339 male cancer patients of Chinese population who were referred to our center from 2010 to 2019 for fertility preservation. Results: We first classify the male tumors into six major types according to body regions. The most prevalent cancer patients who came from our cohort for sperm banking were hematological neoplasms patients, and the second cancers were germ cell tumors. Patients with germ cell tumors had the lowest pre- thaw and post-thaw concentration among the six major cancer types. However, we separately compared among testicular tumors, lymphoma and leukemia, it turned out that leukemia had the lowest pre-thaw concentration. Most cancer patients (58%) chose to go on keeping their specimens in storage. The second proportion selected to discard their specimens electively (31%). Over the years, there were only 13 patients (4%) returned to use their sperm by ART. In the storage samples, germ cell tumors were the most proportion ones (29.3%). Moreover, in the unfrozen samples, the percentage of hematological neoplasms were the most (45.5%).Conclusions: To our knowledge, we had owned the most numbers of male cancers who came to sperm bank for fertility conservation in the southwest of China. In our study we suggested that sperm quality could decrease even before antineoplastic treatment and sperm banking prior to treatment should be strongly recommended for male cancer patients. 

scholarly journals Male cancer patient sperm cryopreservation for fertility preservation: 10-year monocentric experience

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Xiao Liu ◽  
Bo Liu ◽  
Shasha Liu ◽  
Yang Xian ◽  
Wenrui Zhao ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cancer Patients ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Parameters ◽  
Germ Cell Tumours ◽  
Sperm Cryopreservation ◽  
Sperm Banking ◽  
Male Cancer Patient ◽  
Chinese Cancer Patients

Abstract Background Sperm cryopreservation, an effective method for preserving male fertility, is very advantageous for men suffering from cancer. Unfortunately, as both physicians and cancer patients are unaware of the possibilities for sperm cryopreservation, the data on evaluation of semen parameters and disposition of cryopreserved samples among Chinese cancer patients are scarce. Results Male tumours were classified into six major types, germ cell tumours (26 %), haematological neoplasms (28 %), head and neck cancers (19 %), thoracic tumours (4 %), abdominal tumours (10 %), and others (13 %). Haematological neoplasm was the most prevalent cancer among our cohort of patients who opted for sperm banking, followed by germ cell tumours. Patients with germ cell tumours had the lowest pre-thaw and post-thaw seminal sperm concentrations. We separately compared patients with testicular tumours, lymphoma, and leukaemia, and found that leukaemia patients had the lowest pre-thaw sperm concentrations. Most cancer patients (58 %) chose to keep their specimens stored, while 31 % chose to discard the specimens. Over the years, only 13 patients (4 %) returned to use their spermatozoa by assisted reproductive technology. Of the stored samples, patients with germ cell tumours constituted the highest proportion (29.3 %). Moreover, the percentage of haematological neoplasm patients who had no spermatozoa frozen was the highest (46.2 %). Conclusions The present data confirm the deleterious impact of various cancers on semen quality. Leukaemia was associated with the worst semen quality and the highest number of semen samples that could not be frozen. We suggest that sperm quality may have decreased even before anti-neoplastic treatment and that sperm banking before treatment should be strongly recommended for cancer patients. A sperm banking programme before gonadotoxic therapy requires close cooperation between assisted reproduction centres and cancer clinics.

Increase in lactate dehydrogenase isoenzyme-1 in plasma in pediatric malignancy

1990 ◽  
Vol 36 (9) ◽  
pp. 1683-1685 ◽  
Author(s):  
S S Sacks ◽  
G Mulligan
Keyword(s):  
Lactate Dehydrogenase ◽  
Germ Cell ◽  
Cancer Patients ◽  
Acute Lymphocytic Leukemia ◽  
Wilms Tumor ◽  
Germ Cell Tumors ◽  
Lymphocytic Leukemia ◽  
Pediatric Malignancy ◽  
Dehydrogenase Isoenzyme

Abstract We investigated 28 cases of pediatric malignancy in which total lactate dehydrogenase (LD, EC 1.1.1.27) activities were increased and isoenzyme LD-1 exceeded LD-2 (flipped pattern). Of these, 11 had a flipped pattern at presentation and 17 showed a flipped pattern during chemotherapy. Those with flipped patterns at presentation were four with germ-cell tumors, one with acute lymphocytic leukemia, and six with nephroblastomas (Wilms tumor). Four of five nephroblastoma homogenates contained predominantly LD-1; one revealed a structurally normal LD-1 with normal kinetics. We conclude that an increase in LD with a flipped pattern is common in nephroblastoma and, in addition, may develop in cancer patients treated with chemotherapy.

scholarly journals Effects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patients

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 308-313 ◽  
Author(s):  
Seda Karabulut ◽  
Asuman Demiroğlu-Zergeroğlu ◽  
Elif Yılmaz ◽  
Pelin Kutlu ◽  
İlknur Keskin
Keyword(s):  
Dna Fragmentation ◽  
Caspase 3 ◽  
Sperm Quality ◽  
Human Sperm ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Sperm Cryopreservation ◽  
Negative Effects ◽  
Apoptotic Markers ◽  
Neat Semen

SummaryThe negative effects of cryopreservation on sperm parameters are well documented but little information is known about molecular basis of the process. The aim of the present study was to investigate the possible effects of sperm cryopreservation on main apoptotic signs including DNA fragmentation and caspase-3 activation and to determine if these effects vary according to sperm parameters. Sperm samples of 72 patients were cryopreserved. The patients were sub-grouped as normozoospermic or non-normozoospermic patients according to their semen parameters. DNA fragmentation rates and caspase-3 activation levels were analyzed before and after cryopreservation in both groups. Mean DNA fragmentation rate was increased significantly from 23.98% in neat semen samples to 27.34% after cryopreservation (P = 0.03). DNA fragmentation rates were slightly higher in non-normozoospermic patients compared with the normozoospermic patients in both the neat semen and after cryopreservation (23.25 and 24.71% vs. 26.32 and 28.36%, respectively) although the difference obtained were not statistically significant. An increasing trend for caspase-3 activations (0.093 vs. 0.116) was observed after cryopreservation but the differences were not statistically significant. Caspase-3 activation was found to be slightly higher in non-normozoospermic patients both in the neat semen and after cryopreservation compared with the normozoospermic patients but the differences were not statistically significant. Caspase-3 expression was also shown using immunocytochemistry in both fresh ejaculated sperm and thawed sperm after cryopreservation but at different localizations. The cryopreservation process had detrimental effects on sperm quality but the quality of the sperm samples was not adversely effective for the apoptotic markers including DNA fragmentation and caspase-3 activation patterns. In fact, it was the cryopreservation process itself that adversely effected the above apoptotic markers and apoptosis. It was concluded therefore that sperm cell cryopreservation triggers apoptosis after thawing and this process adversely affects semen parameters.

scholarly journals Sperm cryopreservation: Clinical and fertility outcomes in male oncological patients with germ cell tumors or hematological disorders

2018 ◽  
Vol 17 (4) ◽  
pp. 500-503 ◽  
Author(s):  
Hiromitsu Negoro ◽  
Yoshiyuki Matsui ◽  
Takahiro Nakayama ◽  
Hiroshi Hatayama ◽  
Osamu Ogawa ◽  
...  
Keyword(s):  
Germ Cell ◽  
Germ Cell Tumors ◽  
Sperm Cryopreservation ◽  
Hematological Disorders ◽  
Oncological Patients

Risk factors for thromboembolic events in testicular cancer patients receiving chemotherapy

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e16109-e16109
Author(s):  
K. Shim ◽  
K. R. Potvin ◽  
K. Mills ◽  
F. Whiston ◽  
L. Stitt ◽  
...  
Keyword(s):  
Risk Factors ◽  
Germ Cell ◽  
Cancer Patients ◽  
Cell Cancer ◽  
Germ Cell Tumors ◽  
Retrospective Chart Review ◽  
Germ Cell Cancer ◽  
Thromboembolic Events ◽  
Increased Risk ◽  
Risk Of Hemorrhage

e16109 Background: Cancer patients are at increased risk for thromboembolic events (TTEs), and those receiving chemotherapy are at even greater risk. Clinical experience and the literature have suggested that men receiving cisplatin-based chemotherapy for metastatic germ cell tumors are at particularly high risk. As TTEs can be fatal and treatment is curative, the stakes are high. Despite this, prophylactic anticoagulation (PA) is not routinely used. Methods: All men treated with cisplatin-based chemotherapy for metastatic germ cell cancer at the London Regional Cancer Program from January 1978 to December 2007 were identified from electronic databases. Data including type and timing of TTEs were extracted by retrospective chart review. Multivariable analyses were used to identify predictors of TTEs. Results: 196 eligible patients were identified with median age 31 years (range, 15–75). No patients received PA. Thirty-two TTEs were identified in 29 patients for an overall incidence of 14.8% (95% CI, 9.8–19.8%). The majority of events were deep venous thromboses, and five patients died due to TTE or its complications. Sixteen of the patients with TTE (55.2%) were diagnosed while on treatment (defined as TTE within 6 months of chemotherapy initiation); 8 (27.6%) had their TTE prior to, and 5 (17.2%) after this time period. Age greater than 30 years (OR = 3.02; 95% CI, 1.10–8.33; p = 0.033) and elevated LDH (OR = 1.93; 95% CI, 1.07–3.48; p = 0.029) were independently associated with an increased risk of TTE. If both adverse risk factors were present, the risk of TTE on treatment was 21.7% (95% CI, 9.8–33.7%). If neither were present, the negative predictive value was 97% (95% CI, 92–100%). Conclusions: The overall TTE incidence rate of 14.8% is consistent with prior reports (8.4–19%). The risk of TTE appears greatest during chemotherapy and shortly thereafter, and nearly one in 10 patients in this group had a TTE. These data support the concept of PA for selected patients starting chemotherapy for metastatic germ cell cancer. However, the efficacy of PA and risk of hemorrhage in this group is unknown. In this cohort, patients under 30 with normal LDH were at very low risk for TTE. Confirmation of these findings to help guide the study and optimal use of PA should be pursued. No significant financial relationships to disclose.

Sperm Banking for Adolescent and Young Adult Cancer Patients: Sperm Quality, Patient, and Parent Perspectives

2008 ◽  
Vol 50 (3) ◽  
pp. 594-598 ◽  
Author(s):  
Jill P. Ginsberg ◽  
Susan K. Ogle ◽  
Lisa K. Tuchman ◽  
Claire A. Carlson ◽  
Maureen M. Reilly ◽  
...  
Keyword(s):  
Young Adult ◽  
Cancer Patients ◽  
Sperm Quality ◽  
Adolescent And Young Adult ◽  
Parent Perspectives ◽  
Sperm Banking ◽  
Young Adult Cancer

scholarly journals Reproductive Outcomes and Fertility Preservation Strategies in Women with Malignant Ovarian Germ Cell Tumors after Fertility Sparing Surgery

Biomedicines ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 554
Author(s):  
Francesca Maria Vasta ◽  
Miriam Dellino ◽  
Alice Bergamini ◽  
Giulio Gargano ◽  
Angelo Paradiso ◽  
...  
Keyword(s):  
Germ Cell ◽  
Fertility Preservation ◽  
Therapeutic Option ◽  
Germ Cell Tumors ◽  
Oncological Outcome ◽  
Reproductive Age ◽  
Premature Menopause ◽  
Reproductive Outcomes ◽  
Fertility Sparing Surgery ◽  
Fertility Sparing

Malignant ovarian germ cell tumors are rare tumors that mainly affect patients of reproductive age. The aim of this study was to investigate the reproductive outcomes and fertility preservation strategies in malignant ovarian germ cell tumors after fertility-sparing surgery. Data in literature support that fertility-sparing surgery is associated with an excellent oncological outcome not only in early stages malignant ovarian germ cell tumors but also in advanced stages. Moreover, the possibility of performing conservative treatment should be considered even in case of relapse or advanced disease, given the high chemosensitivity. Indeed, available data have shown that menstrual function is maintained after platinum-based regimens in over 85–95% of patients with malignant ovarian germ cell tumors and rate of premature menopause reported in literature ranges between 3% and 7.4%, while premature ovarian failure rates are between 3.4% and 5%. Moreover, reproductive outcomes are about 80% with no increase in the risk of teratogenicity compared to general population. Therefore, conservative surgery for malignant ovarian germ cell tumors currently may represent a therapeutic option in patients who wish to preserve fertility but must be available for extended follow-up and after subscribing to informed consent.

P–441 Semen quality and cryopreservation in adolescent transgender females

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
H Amir ◽  
L Perl ◽  
S Barda ◽  
D Lantsberg ◽  
A Sege. Becker ◽  
...  
Keyword(s):  
Reference Values ◽  
Semen Quality ◽  
Sperm Quality ◽  
Linear Regression Analysis ◽  
Transgender Women ◽  
Semen Parameters ◽  
Control Group ◽  
Multivariate Linear Regression Analysis ◽  
Sperm Cryopreservation ◽  
The Impact

Abstract Study question What are the semen quality and cryopreservation outcomes among adolescent transgender females at the time of fertility preservation (FP) before initiating gender-affirming hormone (GAH) treatment? Summary answer Semen quality is strongly reduced among adolescent transgender females before hormone therapy and their stored sperm samples are suitable for intracytoplasmic sperm injection (ICSI). What is known already The age of individuals seeking treatment for gender affirmation has fallen sharply in recent years and many of them are adolescents. Estrogen, the primary treatment for transgender women, is known to impair semen quality and fertility potential. Sperm cryopreservation enables young transgender females to circumvent GAH therapy-related fertility impairment and have genetically related children. There are recent data on semen quality among adult transgender women who preserve fertility before exposure to GAH therapy, but little is known about pubertal transgender female adolescents. Study design, size, duration This retrospective cohort study included 26 adolescent transgender females who underwent FP between June 2013 and October 2020. Participants/materials, setting, methods Before initiating gonadotropin-releasing hormone agonists solely or with GAH treatment, 25 adolescent transgender females were referred to FP in our Fertility Institute of a tertiary university-affiliated medical center. Pre-freezing semen parameters were compared to WHO 2010 reference values. Post-thaw semen parameters were used to determine adequate assisted reproductive technology (ART). A multivariate linear regression analysis was performed to assess the impact of selected medical and lifestyle factors on the semen quality of our study participants. Main results and the role of chance The mean age at which adolescent transgender females underwent sperm cryopreservation was 16.2 ± 1.38 years. The median values of all semen parameters in our study group were significantly lower compared to the WHO data on semen quality in the general population of unscreened men, including volume (1.46 ml vs 3.2 ml, respectively, P = 0.001 ), sperm concertation (28*106/ml vs 64*106/ml, P < 0.001), total sperm number (28.2*106 vs 196*106, P < 0.001), total motility (51.6% vs 62%, P < 0.001), and normal morphology (2% vs 14%, P < 0.001). The frequency of semen abnormalities was teratozoospermia 72%, hypospermia 52%, oligozoospermia 28%, and azoospermia 4%. The median post-thaw total motile count was 0.17*106 per vial, and the quality was adequate only for ICSI in 87.7% of the thawed semen samples. Attention-deficit/hyperactivity disorder (ADHD) diagnosis, history of depression/anxiety, medication for ADHD, and antidepressant drugs were found to correlate with hypospermia. No correlation was found between the time of FP, body mass index, autistic spectrum disorder diagnosis, cannabis use, testis tucking, or the levels of follicle-stimulating hormone, estradiol, and testosterone on the semen parameters. Limitations, reasons for caution Because no normal values of semen in adolescents are available and the absence of a matched control group, we used WHO 2010 semen data as reference values, and they may not be representative of the adolescent population. Wider implications of the findings: Although adolescent transgender females have poor semen quality and limited stored semen samples suitable for advanced ART interventions, even before starting GAH therapy, we highly recommend sperm cryopreservation before initiating GAH treatment and thereby prevent further impairment of sperm quality associated with the hormonal treatment. Trial registration number Not applicable

scholarly journals Significant Unmet Need in Fertility Counseling and Sperm Cryopreservation in Young Males with Cancer: A Single-Center Analysis

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4488-4488
Author(s):  
Natalie S. Grover ◽  
Allison M. Deal ◽  
Jennifer E. Mersereau ◽  
William A. Wood
Keyword(s):  
Cancer Patients ◽  
Young Male ◽  
Hematologic Malignancies ◽  
Reproductive Age ◽  
Insurance Status ◽  
Sperm Cryopreservation ◽  
Curative Intent ◽  
Sperm Banking ◽  
Fertility Counseling ◽  
Male Patients

Abstract Background: Due to improved cancer survival rates as well as the current trend of delaying childbirth, fertility has become a growing issue among cancer patients. The American Society of Clinical Oncology recommends that oncologists discuss fertility preservation with all patients of reproductive age who are about to initiate treatment that may cause infertility. The purpose of this study was to evaluate the incidence of fertility counseling and sperm banking referral in male cancer patients of reproductive age at our center and to assess whether counseling is influenced by demographics or disease specific factors. Methods: We used the University of North Carolina (UNC) Cancer Registry to identify male patients between the ages of 13-50 who received a new diagnosis of cancer between 1/1/2013 and 1/1/2015 and planned to initiate chemotherapy with curative intent. We excluded patients who initiated chemotherapy outside of UNC. We queried the electronic medical record (EMR) for demographic information such as age, race, insurance status, number of children, marital status, sexual orientation and disease-related variables such as type of cancer, type of treatments, and comorbidities. We reviewed the EMR for documentation of fertility counseling and identified patients who banked sperm at UNC since 1/1/2013. Fisher's Exact and Wilcoxon Rank Sum tests were used to compare characteristics between groups. Additionally, multivariable logistic regression modeling was used to evaluate associations after controlling for age. Results: There were initially 594 male patients identified with new cancer diagnoses, of whom 160 received chemotherapy with curative intent. Of this final cohort of 160 patients, 76 had hematologic malignancies (33 with lymphoma, 30 with acute leukemia, and 13 with other hematologic malignancies). The median age was 42 (40 for hematologic malignancies). 41% of patients had children and 46% were married. Based on chart documentation, 28% of patients were counseled regarding fertility and 13% of patients attempted to bank sperm (46% of those receiving fertility counseling). Rates of counseling and sperm banking were similar for hematologic malignancy patients. Younger patients were significantly more likely to be counseled (p <0.001). Among patients with hematologic malignancies, 51% of patients under 30 years were counseled while 18% of patients ≥ 30 years were counseled (p=0.001). Across all cancer types, insurance type was not associated with fertility counseling (p=0.38) but was associated with attempted sperm banking (p=0.03). Having children, being married, and having a comorbidity were associated with a lower rate of counseling in univariate analysis, but not in multivariate analysis after controlling for age. Conclusions: We found a significant unmet opportunity for fertility counseling and referral for sperm banking in young male cancer patients receiving chemotherapy with curative intent at our center. Low rates of counseling and sperm banking were particularly notable in patients over the age of 30. In addition to barriers in clinical practice, the association between insurance status and sperm banking also suggests a financial barrier to sperm cryopreservation. Further work is needed to develop interventions to improve rates of fertility counseling and opportunities for sperm banking in young male cancer patients. Disclosures Wood: Inform Genomics: Consultancy; Best Doctors: Consultancy.

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