P–441 Semen quality and cryopreservation in adolescent transgender females

Abstract Study question What are the semen quality and cryopreservation outcomes among adolescent transgender females at the time of fertility preservation (FP) before initiating gender-affirming hormone (GAH) treatment? Summary answer Semen quality is strongly reduced among adolescent transgender females before hormone therapy and their stored sperm samples are suitable for intracytoplasmic sperm injection (ICSI). What is known already The age of individuals seeking treatment for gender affirmation has fallen sharply in recent years and many of them are adolescents. Estrogen, the primary treatment for transgender women, is known to impair semen quality and fertility potential. Sperm cryopreservation enables young transgender females to circumvent GAH therapy-related fertility impairment and have genetically related children. There are recent data on semen quality among adult transgender women who preserve fertility before exposure to GAH therapy, but little is known about pubertal transgender female adolescents. Study design, size, duration This retrospective cohort study included 26 adolescent transgender females who underwent FP between June 2013 and October 2020. Participants/materials, setting, methods Before initiating gonadotropin-releasing hormone agonists solely or with GAH treatment, 25 adolescent transgender females were referred to FP in our Fertility Institute of a tertiary university-affiliated medical center. Pre-freezing semen parameters were compared to WHO 2010 reference values. Post-thaw semen parameters were used to determine adequate assisted reproductive technology (ART). A multivariate linear regression analysis was performed to assess the impact of selected medical and lifestyle factors on the semen quality of our study participants. Main results and the role of chance The mean age at which adolescent transgender females underwent sperm cryopreservation was 16.2 ± 1.38 years. The median values of all semen parameters in our study group were significantly lower compared to the WHO data on semen quality in the general population of unscreened men, including volume (1.46 ml vs 3.2 ml, respectively, P = 0.001 ), sperm concertation (28*106/ml vs 64*106/ml, P < 0.001), total sperm number (28.2*106 vs 196*106, P < 0.001), total motility (51.6% vs 62%, P < 0.001), and normal morphology (2% vs 14%, P < 0.001). The frequency of semen abnormalities was teratozoospermia 72%, hypospermia 52%, oligozoospermia 28%, and azoospermia 4%. The median post-thaw total motile count was 0.17*106 per vial, and the quality was adequate only for ICSI in 87.7% of the thawed semen samples. Attention-deficit/hyperactivity disorder (ADHD) diagnosis, history of depression/anxiety, medication for ADHD, and antidepressant drugs were found to correlate with hypospermia. No correlation was found between the time of FP, body mass index, autistic spectrum disorder diagnosis, cannabis use, testis tucking, or the levels of follicle-stimulating hormone, estradiol, and testosterone on the semen parameters. Limitations, reasons for caution Because no normal values of semen in adolescents are available and the absence of a matched control group, we used WHO 2010 semen data as reference values, and they may not be representative of the adolescent population. Wider implications of the findings: Although adolescent transgender females have poor semen quality and limited stored semen samples suitable for advanced ART interventions, even before starting GAH therapy, we highly recommend sperm cryopreservation before initiating GAH treatment and thereby prevent further impairment of sperm quality associated with the hormonal treatment. Trial registration number Not applicable

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Evaluating bull fertility based on non-return method

Veterinarski glasnik ◽

10.2298/vetgl1202027p ◽

2012 ◽

Vol 66 (1-2) ◽

pp. 27-40

Author(s):

Igor Prka ◽

Dragan Vukovic ◽

Stevan Perkovic

Keyword(s):

Semen Quality ◽

Sperm Quality ◽

Quality Parameters ◽

Semen Parameters ◽

Service Period ◽

Microscopic Evaluation ◽

Live Sperm ◽

Return Method ◽

The Impact ◽

First Time

In order to evaluate the results of reproductive cows and heifers, different parameters of fertility are used, such as the service period, insemination index, intercalving time and others, and of the breeding bulls the values obtained through non-return. An ejaculate is taken up for further processing by veterinary centres only provided it meets the prescribed quality parameters. Rating semen parameters includes a macroscopic (volume, colour, consistency, smell and pH) and a microscopic evaluation (mobility, density, percentage of live sperm and abnormal and damaged sperm). In addition to sperm quality and the fertility of the female animal, the results of the non-return method are also influenced by a number of exogenous causes (season, age, race, insemination techniques) that have no small impact on the end result of insemination - pregnancy. In order to obtain more objective results of the fertility of bulls the following tasks were undertaken, namely: 1. to calculate with the non-return method the fertility of bulls in over 10,000 cows inseminated for the first time during a period of 6 years; and 2. to analyze the impact of semen quality, season, age of cow and bull, and the bull breed on the results of fertility.

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Male cancer patient sperm cryopreservation for fertility preservation: 10-year monocentric experience

Basic and Clinical Andrology ◽

10.1186/s12610-021-00140-w ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Xiao Liu ◽

Bo Liu ◽

Shasha Liu ◽

Yang Xian ◽

Wenrui Zhao ◽

...

Keyword(s):

Germ Cell ◽

Cancer Patients ◽

Semen Quality ◽

Sperm Quality ◽

Semen Parameters ◽

Germ Cell Tumours ◽

Sperm Cryopreservation ◽

Sperm Banking ◽

Male Cancer Patient ◽

Chinese Cancer Patients

Abstract Background Sperm cryopreservation, an effective method for preserving male fertility, is very advantageous for men suffering from cancer. Unfortunately, as both physicians and cancer patients are unaware of the possibilities for sperm cryopreservation, the data on evaluation of semen parameters and disposition of cryopreserved samples among Chinese cancer patients are scarce. Results Male tumours were classified into six major types, germ cell tumours (26 %), haematological neoplasms (28 %), head and neck cancers (19 %), thoracic tumours (4 %), abdominal tumours (10 %), and others (13 %). Haematological neoplasm was the most prevalent cancer among our cohort of patients who opted for sperm banking, followed by germ cell tumours. Patients with germ cell tumours had the lowest pre-thaw and post-thaw seminal sperm concentrations. We separately compared patients with testicular tumours, lymphoma, and leukaemia, and found that leukaemia patients had the lowest pre-thaw sperm concentrations. Most cancer patients (58 %) chose to keep their specimens stored, while 31 % chose to discard the specimens. Over the years, only 13 patients (4 %) returned to use their spermatozoa by assisted reproductive technology. Of the stored samples, patients with germ cell tumours constituted the highest proportion (29.3 %). Moreover, the percentage of haematological neoplasm patients who had no spermatozoa frozen was the highest (46.2 %). Conclusions The present data confirm the deleterious impact of various cancers on semen quality. Leukaemia was associated with the worst semen quality and the highest number of semen samples that could not be frozen. We suggest that sperm quality may have decreased even before anti-neoplastic treatment and that sperm banking before treatment should be strongly recommended for cancer patients. A sperm banking programme before gonadotoxic therapy requires close cooperation between assisted reproduction centres and cancer clinics.

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Beneficial Influence of Soybean Lecithin Nanoparticles on Rooster Frozen–Thawed Semen Quality and Fertility

Animals ◽

10.3390/ani11061769 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1769

Author(s):

Lingwei Sun ◽

Mengqian He ◽

Caifeng Wu ◽

Shushan Zhang ◽

Jianjun Dai ◽

...

Keyword(s):

Plasma Membrane ◽

Semen Quality ◽

Antioxidant Activities ◽

Soybean Lecithin ◽

Sperm Quality ◽

The Other ◽

Control Group ◽

Mitochondrial Activity ◽

Computer Assisted ◽

The Impact

The present study aimed to investigate the impact of different concentrations (0%, 0.5%, 1.0%, 1.5%, and 2.0%) of nano-soybean lecithin (SL) in the extender on sperm quality, sperm motion characteristics, and fertility outcomes of post-thawed rooster semen. Adult Ross broiler breeder roosters (n = 20) were subjected to semen collections twice a week for three weeks. At each collection, semen samples were pooled and allocated into five treatments corresponding to different nano-SL concentrations (control, SL0.5, SL1.0, SL1.5, and SL2.0). Sperm parameters, including motility (collected using a computer-assisted sperm analysis system), plasma membrane and acrosome integrities, and mitochondrial activity were assessed. Sperm malondialdehyde (MDA) and antioxidant activities (total antioxidant capacity (TAC); superoxide dismutase (SOD); glutathione peroxidase (GPx)) were evaluated. The fertility and hatchability obtained with frozen–thawed rooster semen supplemented with the optimum nano-SL concentration were assessed after artificial insemination. The results showed that the addition of 1% nano-SL into the extender led to a higher semen motility in roosters, improved plasma membrane and acrosome integrities, and higher mitochondrial activity of post-thawed rooster semen in comparison to controls (p < 0.05). The MDA levels in the SL0.5 and SL1.0 groups were lower than the other groups (p < 0.05). TAC activities in SL0.5, SL1.0, and SL1.5 groups were significantly higher than those in the other groups (p < 0.05). It was observed that the concentration of SOD was higher in the SL1.0 group than in the other groups (p < 0.05). The activity of GPx was not influenced in any of the cases (p > 0.05). Moreover, the percentages of fertility and hatchability in the SL1.0 group were higher (56.36% and 58.06%) than those in the control group (42.72% and 40.43%). In summary, the addition of nano-SL to the extenders enhanced the post-thawed semen quality and fertility of roosters by reducing the level of oxidative stress. The optimum nano-SL concentration was 1.0%. These results may be beneficial for improving the efficacy of semen cryopreservation procedures in poultry breeding.

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Semen Parameters Are Seriously Affected in Acephalic Spermatozoa Syndrome

10.21203/rs.3.rs-993682/v1 ◽

2021 ◽

Author(s):

Li-juan Ying ◽

Qing-ting Liu ◽

Lin Yu ◽

Tingting Yang ◽

Ying-bi Wu ◽

...

Keyword(s):

Case Control Study ◽

Semen Quality ◽

Sperm Quality ◽

Semen Analysis ◽

Semen Parameters ◽

Control Group ◽

Semen Volume ◽

Group A ◽

Group B ◽

Control Study

Abstract Objective: To evaluate the effect of acephalic spermatozoa syndrome on sperm quality in semen with different proportions of headless sperm.Design: Case control study.Setting: Andrology Laboratory.Patient(s): A total of 391 patients with headless sperm and 400 prenatal examination patients with no headless sperm who underwent semen analysis at the andrology laboratory.Intervention(s): None.Main Outcome Measure(s): The correlation of the proportion of headless sperm in semen with semen parameters.Result(s): All semen parameters except the semen volume were negatively (P<0.05) correlated with the proportion of headless sperm in the semen. The semen samples were divided into three groups based on the proportion of headless sperm (PHS) as follows: 0<PHS≤10% (n=249, group A), 10<PHS≤20% (n=71, group B) and PHS>20% (n=71, group C). Nearly all semen parameters were significantly lower in group B and group C than in the control group (P<0.05). However, in group A, only the vitality and motility parameters were lower than those of the control group.Conclusion(s): Semen samples containing headless sperm tend to have lower semen parameters than samples without headless sperm. Increases in the proportion of headless sperm in semen samples are associated with decreases in semen quality.

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The Prevalence of Bacteriospermia in Infertile Men and Association with Semen Quality in Southwestern Iran

Infectious Disorders - Drug Targets ◽

10.2174/1871526519666181123182116 ◽

2020 ◽

Vol 20 (2) ◽

pp. 198-202 ◽

Cited By ~ 1

Author(s):

Mohammad Motamedifar ◽

Yalda Malekzadegan ◽

Parisa Namdari ◽

Behzad Dehghani ◽

Bahia Namavar Jahromi ◽

...

Keyword(s):

Semen Quality ◽

Sperm Quality ◽

Reproductive Function ◽

World Health ◽

Public Health Issue ◽

Control Group ◽

Microbiological Analysis ◽

Male Reproductive Function ◽

Infertile Men ◽

Southwestern Iran

Introduction: Infertility considered as a social and public health issue and estimated that most of these infertile couples are residents of developing countries. Infectious diseases including the history of Sexually Transmitted Infections (STIs) may impact on male reproductive function. Therefore, the present study aimed to investigate the prevalence of bacterial contaminants of semen and probable association with sperm quality of infertile men in Iranian population. Methods: The study population consisted of 200 infertile men and 150 fertile men attending an infertility Center in southwestern Iran during the study period in 2015. The assessment of sperm parameters was according to the World Health Organization (WHO) guidelines. The presumptive pathogens were identified using standard microbiology tests and confirmed by specific PCR primers. Results: The prevalence of bacteriospermia in the semen of the infertile group was significantly higher than that in the fertile group (48% vs. 26.7%, P <0.001). The microbiological analysis of samples showed that the most abundant species of bacteria in semen of infertile men were Chlamydia trachomatis (12.5%) followed by Neisseria gonorrhoeae (11%). On the other hand, in the control group, Lactobacillus spp. (17.3%) was the most isolated pathogen. Results showed that the presence of N. gonorrhoeae, C. trachomatis, Mycoplasma genitalium, Haemophilus, and Klebsiella was significantly associated with sperm abnormality. Conclusion: Based on our findings, it seems that bacteriospermia is associated with alterations in the properties of semen which may lead to a decrease in the fertilization potential of sperm. Therefore, immediate and appropriate treatment is necessary before investigating every other possible cause of infertility.

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What Is the Impact of Excessive Iodine and Semen Quality in Fertile Men of China: An Association Study

10.21203/rs.3.rs-243767/v1 ◽

2021 ◽

Author(s):

Ning Wang ◽

Fang Lv ◽

Ge Yu ◽

Cuige Shi ◽

Shangming Wang ◽

...

Keyword(s):

Semen Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Urinary Iodine ◽

Iodine Concentration ◽

Male Reproduction ◽

Semen Parameters ◽

Human Thyroid ◽

Urinary Iodine Concentration ◽

The Impact

Abstract Background: Iodine is an essential element for hormones synthesized by the human thyroid gland. Thyroid hormone deficiency affects all body tissues, including multiple endocrine changes that alter growth hormones, corticotrophin, glucocorticoids, and gonadal function. In many countries, subfertility is an important problem affecting about 10–15% of all couples trying to conceive. Male subfertile factors have accounted for 25–30% of all cases in their studies. The aim of this study was to study the correlation between urinary iodine concentration (UIC) and semen quality of fertile male in China.Methods: semen and urine samples were collected from males who attended a survey for the reproductive physiological constants of male and female in China. All participants were fertile and they should be free from diseases of male reproductive system and thyroid related diseases. We studied the correlation between UIC and semen quality parameters in fertile males and the effect of iodine concentration on male reproduction. Participants were divided into six groups based on UIC. We measured semen parameters and UIC. Semen parameters or time-to-pregnancy (TTP) were analyzed by Spearman correlation, linear regression and multivariable logistic regression. Results: 1089 men were recruited in the study. The mean and median age of participants were 27.46±0.12 and 27±3.88 years, respectively. UIC of 274 (25.16%) men was lower than the UIC of WHO recommended. UIC of 405 men located in the region of WHO recommended. 62.35% of them were not located in the optimal region. Semen concentration and total sperm count were found to decrease when UIC increases when the UIC≥100 ug/L. Conclusions: A male with deficient UIC or excessive UIC has more risk of lower semen quality such as sperm concentration and total sperm count. In addition, if a male with deficient UIC or excessive UIC, his wife has longer TTP than the males who had normal UIC. Deficient UIC associated with lower semen quality and TTP than excessive UIC.

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Decreased Circulating Insulin-Like Growth Factor 1 Levels Are Associated with Cardiac Diastolic Dysfunction in Small for Gestational Age Infants

American Journal of Perinatology ◽

10.1055/s-0038-1642060 ◽

2018 ◽

Vol 35 (12) ◽

pp. 1178-1185

Author(s):

Shoko Yamazaki ◽

Haruka Obinata ◽

Akira Hachiya ◽

Motoko Kamiya ◽

Noriko Motoki ◽

...

Keyword(s):

Growth Factor ◽

Diastolic Dysfunction ◽

Gestational Age ◽

Small For Gestational Age ◽

Linear Regression Analysis ◽

Entire Population ◽

Doppler Imaging ◽

Multivariate Linear Regression Analysis ◽

Insulin Like Growth Factor ◽

The Impact

Objective To evaluate the impact of serum insulin-like growth factor-1 (IGF-1) levels on cardiac function in small for gestational age (SGA) infants. Study Design This is a prospective, observational study. Serum IGF-1 levels at birth and echocardiography measurements at 1 week of age were compared between SGA and appropriate for gestational age (AGA) infants. Results Thirty-one SGA infants and 27 AGA infants were enrolled. Serum IGF-1 levels were lower in the SGA infants than in the AGA infants. SGA infants had lower mitral lateral annular systolic (S') and early diastolic (E') tissue Doppler imaging velocities compared with AGA infants (S', 5.1 ± 0.9 vs 5.7 ± 1.2 cm/s; E', 6.1 ± 1.5 cm/s vs 7.1 ± 1.3 cm/s; p < 0.05). Serum IGF-1 levels positively correlated with E' velocity in the entire population (r = 0.44, p < 0.001) and in SGA infants (r = 0.39, p < 0.05). In multivariate linear regression analysis, serum IGF-1 and S' velocity were independently associated with E' velocity in the entire population and in SGA infants. Conclusion Decreased serum IGF-I levels could account for cardiac diastolic dysfunction in SGA infants.

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Increased Growth Differentiation Factor 15 in Patients with Hypoleptinemia-Associated Lipodystrophy

International Journal of Molecular Sciences ◽

10.3390/ijms21197214 ◽

2020 ◽

Vol 21 (19) ◽

pp. 7214 ◽

Cited By ~ 1

Author(s):

Susan Kralisch ◽

Annett Hoffmann ◽

Juliane Estrada-Kunz ◽

Michael Stumvoll ◽

Mathias Fasshauer ◽

...

Keyword(s):

Mrna Expression ◽

Transforming Growth Factor ◽

Linear Regression Analysis ◽

Control Group ◽

Serum Concentrations ◽

Metabolic Complications ◽

Growth Differentiation Factor 15 ◽

Differentiation Factor ◽

Obesity And Diabetes ◽

The Impact

Objective. Similar to obesity, lipodystrophy (LD) causes adipose tissue dysfunction and severe metabolic complications. Growth differentiation factor 15 (GDF15) belongs to the transforming growth factor β superfamily and is dysregulated in metabolic disease including obesity and diabetes mellitus. Circulating levels in LD and the impact of leptin treatment have not been investigated so far. Material and Methods. GDF15 serum levels were quantified in 60 LD patients without human immunodeficiency virus infection and 60 controls matched for age, gender, and body mass index. The impact of metreleptin treatment on circulating GDF15 was assessed in a subgroup of patients. GDF15 mRNA expression was determined in metabolic tissues of leptin-deficient lipodystrophic aP2-nSREBP1c-Tg mice, obese ob/ob mice, and control C57Bl6 mice. Results. Median GDF15 serum concentrations were significantly higher in LD patients (819 ng/L) as compared to the control group (415 ng/L) (p < 0.001). In multiple linear regression analysis, an independent and positive association remained between GDF15 on one hand and age, patient group, hemoglobin A1c, triglycerides, and C-reactive protein on the other hand. Moreover, there was an independent negative association between GFD15 and estimated glomerular filtration rate. Circulating GDF15 was not significantly affected by metreleptin treatment in LD patients. Gdf15 was upregulated in leptin-deficient lipodystrophic mice as compared to controls. Moreover, Gdf15 mRNA expression was downregulated by leptin treatment in lipodystrophic and obese animals. Conclusions. Serum concentrations of GDF15 are elevated in LD patients and independently associated with markers of metabolic dysfunction. Gdf15 expression is higher in lipodystrophic mice and downregulated by leptin treatment.

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Association between semen quality among men with different occupational exposures and risk of recurrent spontaneous abortion in island residents

Traditional Medicine and Modern Medicine ◽

10.1142/s257590001950006x ◽

2019 ◽

Vol 02 (02) ◽

pp. 49-57

Author(s):

Wenli Cao ◽

Xinyao Pan ◽

Feijun Ye ◽

Jing Zhou ◽

Zengshu Huang ◽

...

Keyword(s):

Spontaneous Abortion ◽

Semen Quality ◽

Sperm Concentration ◽

Occupational Exposures ◽

Recurrent Spontaneous Abortion ◽

Semen Parameters ◽

Control Group ◽

Effective Prevention ◽

Mechanistic Investigation ◽

Significant Difference

Objective: This study aimed to evaluate the semen quality of male adults whose spouses suffer from recurrent spontaneous abortion (RSA) in the island area, and to explore the association between RSA and occupational exposures. Methods: A total of 131 male patients were recruited and divided into two groups: spouse with recurrent spontaneous abortion group (RSA group, [Formula: see text]) and the normal fertility group (control group, [Formula: see text]). Information such as height, weight and occupational exposure history of 131 men were obtained. Semen samples were collected and analyzed. Differences in semen parameters and DNA fragmentation index (DFI) between the two groups were compared. Odds ratios (ORs) and their corresponding 95% confidence intervals were calculated to evaluate the association between occupational exposures and RSA status. Results: Overall, no significant difference was found in sperm concentration, progressive motility and normal morphology rate between RSA and control groups. Only DFI was observed to be significantly higher in the RSA group. Evaluation of the receiver operating characteristic (ROC) curve showed DFI (AUC: 0.623, [Formula: see text]) could discriminate between males from the couples with RSA and without RSA. Conclusion: The analysis of conventional semen parameters could not directly reflect their influence on embryonic development, and test of integrity of the sperm DNA is of paramount importance to fully understand male fertility. It is crucial to conduct studies regarding occupational exposures and pregnancy loss and/or RSA risk, since it will provide population-level data to aid in the identification of important risk factors that warrant further mechanistic investigation, and eventually lead to effective prevention and treatment strategies.

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Bone dehiscence formation during orthodontic tooth movement through atrophic alveolar ridges

The Angle Orthodontist ◽

10.2319/063019-443.1 ◽

2019 ◽

Vol 90 (3) ◽

pp. 321-329

Author(s):

Adilson Luiz Ramos ◽

Monique Cimão dos Santos ◽

Márcio Rodrigues de Almeida ◽

Carlos Flores Mir

Keyword(s):

Null Hypothesis ◽

Alveolar Bone ◽

Tooth Movement ◽

Linear Regression Analysis ◽

Orthodontic Tooth Movement ◽

Wilcoxon Test ◽

Control Group ◽

Multivariate Linear Regression Analysis ◽

Alveolar Ridge ◽

Bone Height

ABSTRACT Objectives To test the null hypothesis that there is no difference in bone dehiscence formation before and after orthodontic tooth movement through an atrophic alveolar ridge. Material and Methods This longitudinal retrospective study evaluated pretreatment and posttreatment cone-beam computed tomography imaging of 15 adult patients. Twenty-five teeth were moved through the atrophic alveolar bone, whereas 25 teeth not subjected to translational movement were considered controls. The distances between the cementoenamel junction and the alveolar bone crest were assessed at the mesial, distal, buccal, and lingual surfaces of all of these teeth. Data were compared using the Wilcoxon test. The Spearman correlation test and multivariate linear regression analysis were also performed. Results In general, crestal bone height was reduced around 0.5 mm in all groups in every direction. Median buccal dehiscence increased significantly (+2.25 mm) (P < .05) in teeth moved through the atrophic ridge. Control teeth also had buccal crest loss (+0.83 mm), but this was not statistically different from that of the experimental teeth. Lingual dehiscence increased significantly for the experimental (+0.17 mm) and control (+0.65 mm) groups. Mesial bone height decreased more in the control group (–0.44mm) than in the experimental group (–0.14mm). There was moderate correlation between amount of tooth movement and alveolar bone loss. Conclusions The null hypothesis was rejected as dehiscence increased after tooth movement through an atrophic alveolar ridge, mainly in the buccal plate.

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