scholarly journals Identification and fine mapping of a novel qGR6.2 locus controlling rice salt tolerance during seed germination

2020 ◽  
Author(s):  
Peng Zeng ◽  
Peiwen Zhu ◽  
Luofeng Qian ◽  
Xumei Qian ◽  
Yuxin Mi ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Candidate Genes ◽  
Fine Mapping ◽  
Developmental Stages ◽  
Indica Rice ◽  
Germination Rate ◽  
Differentially Expressed ◽  
Germination Ability ◽  
Germination Traits

Abstract Background Rice growth is frequently affected by salinity. When rice plants are exposed to high salinity, seed germination and seedling establishment are significantly inhibited. In particular, with the promotion of rice direct-seeding in Asia, improving rice salt tolerance during seed germination is of strong importance for rice breeding. Results In this study, we found that the indica rice landrace Wujiaozhan (WJZ) showed a high capability of seed germination under both water (H 2 O) and salt (NaCl) conditions, particularly under high salt stress. The BC 1 F 2 population produced by crossing WJZ with japonica Nipponbare (Nip) was used to evaluate the germination traits under water (H 2 O) and salt (300 mM NaCl) conditions using germination rate (GR) and germination index (GI). A total of 13 quantitative trait loci (QTLs) were identified, including eight QTLs of GR, two QTLs of GI under H 2 O conditions, six QTLs of GR, and three QTLs of GI under 300 mM NaCl conditions. Six QTLs ( qGR6.1 , qGR8.1 , qGR8.2 , qGR10.1 , qGR10.2 and qGI10.1 ) contributed to GR under both H 2 O and 300 mM NaCl conditions. Three QTLs ( qGR6.2 , qGR10.1 and qGR10.2 ) under 300 mM NaCl conditions were identified at different time points of seed germination and shared the same region with qGI6 , qGI10.1 and qGI10.2 for GI. These QTLs could be used to improve seed germination ability via marker-assisted selection (MAS). One major effective salt-tolerance-specific QTL, qGR6.2, on chromosome 6 was further confirmed via the BC 2 F 2 population, which explained more than 20% of the phenotypic variation. Fine mapping results showed that qGR6.2 was narrowed to a 65.9-kb region between the Z654 and Z619 molecular markers, with eleven candidate genes being predicted. Based on the microarray database, there were high transcript abundances of six genes ( LOC_Os06g10650 , LOC_Os06g10660 , LOC_Os06g10690 , LOC_Os06g10710 , LOC_Os06g10730 and LOC_Os06g10750 ) at all developmental stages, and only LOC_Os06g10750 was differentially expressed after salt incubation. RT-qPCR showed that two genes ( LOC_Os06g10650 and LOC_Os06g10750 ) were significantly differentially expressed at 300 mM NaCl during seed germination. This result suggested that LOC_Os06g10650 and LOC_Os06g10750 might be the causal candidate genes for the major effective salt-tolerance-specific QTL qGR6.2 identified in WJZ, which may facilitate map-based cloning and help to elucidate the molecular mechanism underlying salt tolerance during seed germination. Conclusions In our study, we identified 13 QTLs from indica landrace WJZ that confer seed germination traits under water and salt conditions. A major salt-tolerance-specific QTL qGR6.2 was confirmed and fine mapped to a 65.9-kb region flanked by the Z654 and Z619 markers. Our results provide information on the genetic basis of improving salt tolerance during seed germination by MAS.

scholarly journals Identification and fine mapping of a novel qGR6.2 locus controlling rice salt tolerance during seed germination

2020 ◽  
Author(s):  
Peng Zeng ◽  
Peiwen Zhu ◽  
Luofeng Qian ◽  
Xumei Qian ◽  
Yuxin Mi ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Candidate Genes ◽  
Fine Mapping ◽  
Developmental Stages ◽  
Indica Rice ◽  
Germination Rate ◽  
Differentially Expressed ◽  
Germination Ability ◽  
Germination Traits

Abstract Background Rice growth is frequently affected by salinity. When rice plants are exposed to high salinity, seed germination and seedling establishment are significantly inhibited. In particular, with the promotion of rice direct-seeding in Asia, improving rice salt tolerance during seed germination is of strong importance for rice breeding. Results In this study, we found that the indica rice landrace Wujiaozhan (WJZ) showed a high capability of seed germination under both water (H 2 O) and salt (NaCl) conditions, particularly under high salt stress. The BC 1 F 2 population produced by crossing WJZ with japonica Nipponbare (Nip) was used to evaluate the germination traits under water (H 2 O) and salt (300 mM NaCl) conditions using germination rate (GR) and germination index (GI). A total of 13 quantitative trait loci (QTLs) were identified, including eight QTLs of GR, two QTLs of GI under H 2 O conditions, six QTLs of GR, and three QTLs of GI under 300 mM NaCl conditions. Six QTLs ( qGR6.1 , qGR8.1 , qGR8.2 , qGR10.1 , qGR10.2 and qGI10.1 ) contributed to GR under both H 2 O and 300 mM NaCl conditions. Three QTLs ( qGR6.2 , qGR10.1 and qGR10.2 ) under 300 mM NaCl conditions were identified at different time points of seed germination and shared the same region with qGI6 , qGI10.1 and qGI10.2 for GI. These QTLs could be used to improve seed germination ability via marker-assisted selection (MAS). One major effective salt-tolerance-specific QTL, qGR6.2, on chromosome 6 was further confirmed via the BC 2 F 2 population, which explained more than 20% of the phenotypic variation. Fine mapping results showed that qGR6.2 was narrowed to a 65.9-kb region between the Z654 and Z619 molecular markers, with eleven candidate genes being predicted. Based on the microarray database, there were high transcript abundances of six genes ( LOC_Os06g10650 , LOC_Os06g10660 , LOC_Os06g10690 , LOC_Os06g10710 , LOC_Os06g10730 and LOC_Os06g10750 ) at all developmental stages, and only LOC_Os06g10750 was differentially expressed after salt incubation. RT-qPCR showed that two genes ( LOC_Os06g10650 and LOC_Os06g10750 ) were significantly differentially expressed at 300 mM NaCl during seed germination. This result suggested that LOC_Os06g10650 and LOC_Os06g10750 might be the causal candidate genes for the major effective salt-tolerance-specific QTL qGR6.2 identified in WJZ, which may facilitate map-based cloning and help to elucidate the molecular mechanism underlying salt tolerance during seed germination. Conclusions In our study, we identified 13 QTLs from indica landrace WJZ that confer seed germination traits under water and salt conditions. A major salt-tolerance-specific QTL qGR6.2 was confirmed and fine mapped to a 65.9-kb region flanked by the Z654 and Z619 markers. Our results provide information on the genetic basis of improving salt tolerance during seed germination by MAS.

scholarly journals Identification and fine mapping of a novel qGR6.2 locus controlling rice salt tolerance during seed germination

2020 ◽  
Author(s):  
Peng Zeng ◽  
Peiwen Zhu ◽  
Luofeng Qian ◽  
Zefeng Lin ◽  
Xumei Qian ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Qtl Mapping ◽  
Ssr Markers ◽  
Fine Mapping ◽  
Bulked Segregant Analysis ◽  
Indica Rice ◽  
Germination Rate ◽  
Expression Patterns

Abstract The rice growth is strongly affected by salt stress. When exposed to high salt stress, seed germination and seedling establishment are significantly inhibited. Particularly, with the promotion of rice direct-seeding technology in Asia, improving rice salt tolerance during seed germination is of great importance for rice breeding. In this study, an indica rice landrace Wujiaozhan (WJZ) showed significant salt tolerance during seed germination. The dynamic process of seed germination at 300 mM NaCl was observed via the germination rate (GR), seedling percentage (SP), and germination index (GI) from the progeny by crossing WJZ with the salt-sensitive Nipponbare (Nip). By QTL mapping, sixteen quantitative trait loci (QTLs) related to salt-tolerance were detected via a BC1F2 population, including six QTLs for the GR, eight QTLs for the SP, and two QTLs for the GI. Of them, four QTLs, qGR6.1, qGR6.2, qGR10 and qSP10.1, were expressed continuously, especially qGR6.2 and qGR10. Furthermore, twelve significant associated simple sequence repeats (SSR) markers were detected in the F2 population by bulked segregant analysis (BSA). Four SSR markers (RM588, RM190, RM276 and RM5493) were closed to qGR6.1, qGR6.2, qGR6.3, qSP6.1, qSP6.2, qGI6 and qSP8.1 identified by QTL mapping. The major qGR6.2 on chromosome 6 was confirmed via the BC2F2 population, which explained more than 20% phenotype variation of the GR. Fine mapping results displayed that qGR6.2 was narrowed down to a 65.9 kb region between the Z654 and Z619 markers, with eleven candidate genes predicted. Based on the microarray database, six genes (LOC_Os06g10650, LOC_Os06g10660, LOC_Os06g10690, LOC_Os06g10710, LOC_Os06g10730 and LOC_Os06g10750) were differentially expressed after seed imbibition or salt stress. RT-qPCR showed that two genes (LOC_Os06g10650 and LOC_Os06g10750) were significantly induced by salt stress and had different expression patterns in two parents during seed germination.

scholarly journals Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peng Zeng ◽  
Peiwen Zhu ◽  
Luofeng Qian ◽  
Xumei Qian ◽  
Yuxin Mi ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Candidate Genes ◽  
Germination Rate ◽  
Tyrosine Phosphatase ◽  
Rice Seed ◽  
Microarray Database ◽  
Family Protein ◽  
Germination Traits ◽  
Causal Candidate

Abstract Background Rice growth is frequently affected by salinity. When exposed to high salinity, rice seed germination and seedling establishment are significantly inhibited. With the promotion of direct-seeding in Asia, improving rice seed germination under salt stress is crucial for breeding. Results In this study, an indica landrace Wujiaozhan (WJZ) was identified with high germinability under salt stress. A BC1F2 population derived from the crossing WJZ/Nip (japonica, Nipponbare)//Nip, was used to quantitative trait loci (QTL) mapping for the seed germination rate (GR) and germination index (GI) under H2O and 300 mM NaCl conditions. A total of 13 QTLs were identified, i.e. ten QTLs under H2O conditions and nine QTLs under salt conditions. Six QTLs, qGR6.1, qGR8.1, qGR8.2, qGR10.1, qGR10.2 and qGI10.1 were simultaneously identified under two conditions. Under salt conditions, three QTLs, qGR6.2, qGR10.1 and qGR10.2 for GR were identified at different time points during seed germination, which shared the same chromosomal region with qGI6.2, qGI10.1 and qGI10.2 for GI respectively. The qGR6.2 accounted for more than 20% of phenotypic variation under salt stress, as the major effective QTL. Furthermore, qGR6.2 was verified via the BC2F2 population and narrowed to a 65.9-kb region with eleven candidate genes predicted. Based on the microarray database, five candidate genes were found with high transcript abundances at the seed germination stage, of which LOC_Os06g10650 and LOC_Os06g10710 were differentially expressed after seed imbibition. RT-qPCR results showed the expression of LOC_Os06g10650 was significantly up-regulated in two parents with higher levels in WJZ than Nip during seed germination under salt conditions. Taken together, it suggests that LOC_Os06g10650, encoding tyrosine phosphatase family protein, might be the causal candidate gene for qGR6.2. Conclusions In this study, we identified 13 QTLs from a landrace WJZ that confer seed germination traits under H2O and salt conditions. A major salt-tolerance-specific QTL qGR6.2 was fine mapped to a 65.9-kb region. Our results provide information on the genetic basis of improving rice seed germination under salt stress by marker-assisted selection (MAS).

Responses of seed germination, seedling growth under salinity stresses and variability for phenotypic traits in Tossa Jute (Corchorus olitorius L.)

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Md. Mia Mukul ◽  
Sheikh Shorif Uddin Ahmed ◽  
Nargis Akter ◽  
Md. Golam Mostofa ◽  
Md. Sohanur Rahman ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Germination Rate ◽  
Phenotypic Traits ◽  
Tolerance Index ◽  
Corchorus Olitorius ◽  
Salt Tolerant ◽  
Fiber Yield ◽  
Good For ◽  
Salt Tolerance Index

Salinity is a serious abiotic stress to Jute and other crop cultivation at saline regions in the world. No salt tolerant Tossa Jute (Corchorus olitorius L.) variety was developed in Bangladesh. Hence, six Tossa Jute accessions were investigated at germination stage against six concentration levels (0.00 or d.H2O, 8.0, 10.0, 12.0, 14.0 and 16.0 dS m-1) of salt (NaCl) using RCB design at Bangladesh Jute Research Institute (BJRI) during March-July, 2020. Jute seeds collected from Gene Bank of BJRI were allowed to germinate under laboratory condition. Seed germination rate was adversely affected as well as delay in germination was prolonged with increasing the salt concentration. In control, seeds were germinated up to 14.0 dS m-1 salt solution. Among six genotypes, Acc. 1141 and Acc. 3801 showed the highest germination rate (86.67 %); Acc. 3801 gave maximum root length (17.0 mm), dry biomass (6.37 mg); and Acc. 1089 showed higher shoot length (10.0 mm), fresh weight (43.93 mg) and salt tolerance index (60.69 %) under 14.0 dS m-1 level. Higher relative salt harm rate (7.14 %) was observed in both Acc. 1141 and Acc. 3801 under 14.0 dS m-1 salinity indicating highly tolerance to salinity. Acc. 3801 and Acc. 1141 were found good for germination under salt stresses; Acc. 3801, Acc. 1089 for fiber yield and salt tolerance; Acc. 3801 and Acc. 1407 for higher fiber yield. Acc. 3801 was found good for salt tolerance and fiber yield content. The genotypes with good desirable characters would be used as breeding materials to develop high yielding salt tolerant Tossa Jute variety.

scholarly journals Combined Analysis of the Metabolome and Transcriptome Identified Candidate Genes Involved in Phenolic Acid Biosynthesis in the Leaves of Cyclocarya paliurus

2020 ◽  
Vol 21 (4) ◽  
pp. 1337 ◽  
Author(s):  
Weida Lin ◽  
Yueling Li ◽  
Qiuwei Lu ◽  
Hongfei Lu ◽  
Junmin Li
Keyword(s):  
Transcription Factors ◽  
Candidate Genes ◽  
Phenolic Acid ◽  
Developmental Stages ◽  
Ultra Performance Liquid Chromatography ◽  
Differentially Expressed ◽  
Regulation Mechanism ◽  
Acid Biosynthesis ◽  
Cyclocarya Paliurus ◽  
Helix Loop Helix

To assess changes of metabolite content and regulation mechanism of the phenolic acid biosynthesis pathway at different developmental stages of leaves, this study performed a combined metabolome and transcriptome analysis of Cyclocarya paliurus leaves at different developmental stages. Metabolite and transcript profiling were conducted by ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometer and high-throughput RNA sequencing, respectively. Transcriptome identification showed that 58 genes were involved in the biosynthesis of phenolic acid. Among them, 10 differentially expressed genes were detected between every two developmental stages. Identification and quantification of metabolites indicated that 14 metabolites were located in the phenolic acid biosynthetic pathway. Among them, eight differentially accumulated metabolites were detected between every two developmental stages. Association analysis between metabolome and transcriptome showed that six differentially expressed structural genes were significantly positively correlated with metabolite accumulation and showed similar expression trends. A total of 128 transcription factors were identified that may be involved in the regulation of phenolic acid biosynthesis; these include 12 MYBs and 10 basic helix–loop–helix (bHLH) transcription factors. A regulatory network of the phenolic acid biosynthesis was established to visualize differentially expressed candidate genes that are involved in the accumulation of metabolites with significant differences. The results of this study contribute to the further understanding of phenolic acid biosynthesis during the development of leaves of C. paliurus.

Comparative studies on salt tolerance of seedlings for one cultivar of puccinellia (Puccinellia ciliata) and two cultivars of tall wheatgrass (Thinopyrum ponticum)

2005 ◽  
Vol 45 (4) ◽  
pp. 391 ◽  
Author(s):  
B. Zhang ◽  
B. C. Jacobs ◽  
M. O'Donnell ◽  
J. Guo
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Salt Tolerant ◽  
Mineral Contents ◽  
Shoot Height ◽  
Thinopyrum Ponticum ◽  
Tall Wheatgrass

Salt tolerances of 3 cultivars, Menemen puccinellia (Puccinellia ciliata Bor), Tyrrell and Dundas [tall wheatgrass, Thinopyrum ponticum (Podp.) Z. W. Liu and R. R. C. Wang], were compared with respect to their seed germination, adaptive responses to salt and waterlogging, seedling emergence, plant growth, shoot osmolality and mineral contents in a series of salt-stress experiments. An inverse normal distribution provided good fits for the time to seed germination. Under NaCl stress, 50% of the control (distilled water) seed germination rates of Menemen, Tyrrell and Dundas were achieved in 178.8, 300.9 and 296.8 mmol/L NaCl, respectively. Fifty percent of the control seedling emergence rates of these 3 cultivars were in 92.7, 107.2 and 113.5 mmol/L NaCl, respectively. The seed germination rates of these 3 cultivars under both salt and waterlogging stress were far lower than those germinated only under salt stress at the same salt level. Seed pretreatment by soaking seed in NaCl solutions greatly increased the seed germination rate under salt stress for Menemen and under both salt stress and waterlogging for Dundas. Tyrrell and Dundas were very similar in their tolerance to salt stress, and were significantly (P<0.05) more salt tolerant than Menemen in terms of seed germination and seedling emergence rate. Both shoot height and dry matter of these 3 cultivars were not statistically different among all salt stress levels during the seedling elongation period, indicating that the established plants of these 3 cultivars were very salt tolerant. The salt tolerance mechanisms of these 3 cultivars are possibly related to their abilities to maintain high osmolality in shoots by regulating high sodium and potassium contents, and reducing calcium deficiency under salt stress.

scholarly journals Genome-wide association and differential expression analysis of salt tolerance in Gossypium hirsutum L at the germination stage

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yanchao Yuan ◽  
Huixian Xing ◽  
Wenguan Zeng ◽  
Jialing Xu ◽  
Lili Mao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Candidate Genes ◽  
Upland Cotton ◽  
Differentially Expressed ◽  
Genome Wide Association ◽  
Rna Seq ◽  
Salt Tolerant ◽  
Genome Wide ◽  
Germination Stage

Abstract Background Salinity is a major abiotic stress seriously hindering crop yield. Development and utilization of tolerant varieties is the most economical way to address soil salinity. Upland cotton is a major fiber crop and pioneer plant on saline soil and thus its genetic architecture underlying salt tolerance should be extensively explored. Results In this study, genome-wide association analysis and RNA sequencing were employed to detect salt-tolerant qualitative-trait loci (QTLs) and candidate genes in 196 upland cotton genotypes at the germination stage. Using comprehensive evaluation values of salt tolerance in four environments, we identified 33 significant single-nucleotide polymorphisms (SNPs), including 17 and 7 SNPs under at least two and four environments, respectively. The 17 stable SNPs were located within or near 98 candidate genes in 13 QTLs, including 35 genes that were functionally annotated to be involved in salt stress responses. RNA-seq analysis indicated that among the 98 candidate genes, 13 were stably differentially expressed. Furthermore, 12 of the 13 candidate genes were verified by qRT-PCR. RNA-seq analysis detected 6640, 3878, and 6462 differentially expressed genes at three sampling time points, of which 869 were shared. Conclusions These results, including the elite cotton accessions with accurate salt tolerance evaluation, the significant SNP markers, the candidate genes, and the salt-tolerant pathways, could improve our understanding of the molecular regulatory mechanisms under salt stress tolerance and genetic manipulation for cotton improvement.

scholarly journals A Novel Stable QTL in Chromosome A04 for Salt Tolerance at the Seed Germination Stage of Upland Cotton via a Resequencing-Based High-Density Genetic Map

2021 ◽  
Author(s):  
Qishen Gu ◽  
Huifeng Ke ◽  
Chenchen Liu ◽  
Xing Lv ◽  
Zhengwen Sun ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Candidate Genes ◽  
Genetic Map ◽  
Germination Rate ◽  
High Density ◽  
Virus Induced Gene Silencing ◽  
Stable Quantitative Trait Locus ◽  
Trait Locus ◽  
Functional Analyses

Abstract Key message Two candidate genes GhGASA1 and GhADC2 playing negative roles by modulating the GA and PA signaling pathway, respectively, were identified in a major QTL for germination under salt stress.The successful transition of a seed into a seedling is the prerequisite for plant propagation and crop yield. Germination is a vulnerable stage in a plant’s life cycle which is strongly affected by environmental conditions, such as salinity. In this study, we identified a novel stable quantitative trait locus (QTL) qRGR-A04-1 associated with relative germination rate (RGR) after treatment with salt stress based on a high-density genetic map under phytotron and filed conditions, with LOD values of 6.65-16.83 and 6.11-12.63% of phenotypic variations in all five environment tests. Two candidate genes with significantly differential expression between two parents were finally identified through RNA-seq and qRT-PCR analyses. Further functional analyses showed that GhGASA1- and GhADC2-overexpression lines were more sensitive to salt stress than wild-type in Arabidopsis through regulating the transcript levels of gibberellic acid (GA) and polyamine (PA) -related genes implicating in GA and PA biosynthesis with reducing the accumulation of GA and PA under salt stress, respectively. Virus-induced gene silencing analysis showed that TRV:GASA1 and TRV:ADC2 displayed more tolerant to salt stress by increasing the expression of GA-synthesis genes and decreasing the H2O2 content, respectively. Taken together, our results suggested that QTL qRGR-A04-1 and its harbored two genes, GhGASA1 and GhADC2, were promising candidates for salt tolerance improvement in cotton.

Comparison of salt tolerance during seed germination and vegetative growth in tomato by QTL mapping

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 727-734 ◽  
Author(s):  
M R Foolad
Keyword(s):  
Quantitative Trait Loci ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Lycopersicon Esculentum ◽  
Quantitative Trait ◽  
Vegetative Growth ◽  
Developmental Stages ◽  
Phenotypic Correlation ◽  
Recurrent Parent ◽  
Trait Loci

The purpose of this study was to determine the genetic relationship between salt tolerance during seed germination and vegetative growth in tomato by comparing quantitative trait loci (QTLs) which confer salt tolerance at these two developmental stages. A salt-sensitive Lycopersicon esculentum line (NC84173; maternal and recurrent parent) was hybridized with a salt-tolerant accession (LA722) of Lycopersicon pimpinellifolium, and BC1 and BC1S1 populations were developed. The BC1 population was used for RFLP mapping and the BC1S1 population for evaluation of salt tolerance during germination and vegetative growth. The results indicated the presence of a small but significant correlation (r = -0.22, p < 0.05) between rate of seed germination and the percentage of plant survival under salt stress. Seven and five QTLs were identified for salt tolerance during seed germination and vegetative growth, respectively. While in most cases the location of QTLs for germination was different from that for vegetative growth, there were some coincidences in QTL locations; this was consistent with the small phenotypic correlation observed between the two traits. The overall results indicated that, in these tomato genetic materials, salt tolerance during seed germination was independent of that during vegetative growth. However, simultaneous improvement of tolerance at the two developmental stages should be possible through marker-assisted selection and breeding.Key words: Lycopersicon esculentum, L. pimpinellifolium, salt tolerance, seed germination vegetative growth, restriction fragment length polymorphism (RFLP), quantitative trait loci (QTLs).

scholarly journals Salt Resistance of Tomato (Lycopersicon esculentum Mill.) Cultivars Produced in Benin at Germination Stage

2019 ◽  
pp. 1-12
Author(s):  
Eliane Kinsou ◽  
David Montcho ◽  
Séraphin Ahissou Zanklan ◽  
Julien Koffi Kpinkoun ◽  
Françoise Assogba Komlan ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Seed Germination ◽  
Germination Rate ◽  
Nacl Stress ◽  
Salt Resistance ◽  
Tolerance Index ◽  
Resistance Level ◽  
Significant Difference ◽  
Salt Tolerance Index ◽  
Germination Stage

Aims: In this research study, salt resistance level of seven tomato cultivars grown in Benin, namely Akikon, Tounvi; F1 Mongal, Petomech, Padma, TLCV 15 and Thorgal was evaluated at the germination stage. Study Design: The experiment was laid out as a completely randomized design with four replications. Place and Duration of Study: The experiment was carried out in the Laboratory of Plant Physiology and Abiotic Stresses Study of University of Abomey-Calavi, Republic of Benin from May to June, 2017. Methodology: Seeds were submitted to treatment with four NaCl concentrations (0; 30; 60 and 90 mM NaCl) in Petri dishes. Seed germination was checked every day during ten days incubation period. Four replicates of 40 seeds each were used. Results: NaCl reduced seed germination rate in all cultivars from day 2 to day 10 and the germination index proportionately to NaCl concentration. At the end of the 10 days, salt stress reduced the final germination percentages with a significant difference among cultivars: cultivars F1 Mongal followed by Akikon, Thorgal, TLCV15 and Tounvi were less affected in comparison with the two other cultivars. Salt Tolerance Index was significantly variable according to the cultivar with the highest values for cultivars F1 Mongal (1.086), Akikon (1.028), TLCV15 (1.005) and Tounvi (0.989) and the weakest value for cultivar Petomech (0.436). Conclusion: NaCl stress delayed seed germination and reduced the rate of final germination. Salt Tolerance Index was variable among the seven cultivars. Based on this criterion, cultivars F1 Mongal, Akikon, TLCV15 and Tounvi were the most salt-resistant whereas Petomech was the most salt-sensitive at germination stage.

Sign in / Sign up

Export Citation Format

Share Document