scholarly journals Sex-dependent dysregulation of human neutrophils responses​ by bisphenol A

2020 ◽  
Author(s):  
Wioletta Ratajczak-Wrona ◽  
Marzena Garley ◽  
Malgorzata Rusak ◽  
Karolina Nowak ◽  
Jan Czerniecki ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Phagocytic Activity ◽  
Human Neutrophils ◽  
No Production ◽  
Physiological Concentration ◽  
Study Objective ◽  
Environmental Concentration ◽  
Pathogen Elimination ◽  
The Impact

Abstract Background: The study objective was to assess the impact of a xenoestrogen—bisphenol A (BPA) (at the environmental concentration and 100-fold increased dose)—on selected functions of neutrophils, as well as to compare the effect of this compound and 17-β-estradiol (E2) (at the physiological concentration) on the studied functions of these cells in women and men.Methods and Results: Analysis of the results obtained for both sexes demonstrated a reduction in the chemotactic potential of neutrophils after exposure to BPA. In the presence of BPA, the phagocytic activity, as measured via the SCORE index, was found to be elevated in the neutrophils from women and reduced in those isolated for men. Exposure to BPA resulted in an increased percentage of neutrophils expressing CD14 and CD284 (TLR4), as well as an increased percentage of NET-forming cells, in the case of both sexes. In addition, a reduced capacity to release NETs was observed in cells that were preincubated with xenoestrogen and then stimulated with LPS. The stimulating role of BPA and 17-β-estradiol in the activation of NADPH oxidase, evaluated using NBT stimulation test, was evident only in the neutrophils from women. No influence of E2 could be observed on the expression of CD14 and CD284 by neutrophils, their chemotactic potential and phagocytic activity, and the amount of NETs for both sexes. The study further showed that BPA intensified the NO production and iNOS expression in the neutrophils from both sexes. In addition, an increase in expression was found for all the tested proteins of the PI3K/AKT pathway for men.Conclusions: The conducted study demonstrated that BPA influenced the functions of neutrophils associated with locomotion and pathogen elimination, which may disturb the response of these cells in both women and men. Furthermore, neutrophils isolated from women were found to be more susceptible to the effect of BPA in terms of oxygen-dependent killing, compared to those obtained from men. The variable results of the tests conducted in the presence of BPA indicated the stronger effect of xenoestrogen on human neutrophils compared to that of 17-β-estradiol.

scholarly journals Sex-dependent dysregulation of human neutrophil responses by bisphenol A

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Wioletta Ratajczak-Wrona ◽  
Marzena Garley ◽  
Malgorzata Rusak ◽  
Karolina Nowak ◽  
Jan Czerniecki ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Bisphenol A ◽  
Total Concentration ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Human Neutrophils ◽  
Boyden Chamber ◽  
No Production ◽  
Pathogen Elimination ◽  
Latex Beads

Abstract Background In the present study, we aimed to investigate selected functions of human neutrophils exposed to bisphenol A (BPA) under in vitro conditions. As BPA is classified among xenoestrogens, we compared its action and effects with those of 17β-estradiol (E2). Methods Chemotaxis of neutrophils was examined using the Boyden chamber. Their phagocytosis and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase activity were assessed via Park’s method with latex beads and Park’s test with nitroblue tetrazolium. To assess the total concentration of nitric oxide (NO), the Griess reaction was utilized. Flow cytometry was used to assess the expression of cluster of differentiation (CD) antigens. The formation of neutrophil extracellular traps (NETs) was analyzed using a microscope (IN Cell Analyzer 2200 system). Expression of the investigated proteins was determined using Western blot. Results The analysis of results obtained for both sexes demonstrated that after exposure to BPA, the chemotactic capacity of neutrophils was reduced. In the presence of BPA, the phagocytic activity was found to be elevated in the cells obtained from women and reduced in the cells from men. Following exposure to BPA, the percentage of neutrophils with CD14 and CD284 (TLR4) expression, as well as the percentage of cells forming NETs, was increased in the cells from both sexes. The stimulatory role of BPA and E2 in the activation of NADPH oxidase was observed only in female cells. On the other hand, no influence of E2 on the expression of CD14 and CD284, chemotaxis, phagocytosis, and the amount of NET-positive neutrophils was found for both sexes. The study further showed that BPA intensified NO production and iNOS expression in the cells of both sexes. In addition, intensified expression of all tested PI3K-Akt pathway proteins was observed in male neutrophils. Conclusions The study demonstrated the influence of BPA on neutrophil functions associated with locomotion and pathogen elimination, which in turn may disturb the immune response of these cells in both women and men. Analysis of the obtained data showed that the effect of this xenoestrogen on the human neutrophils was more pronounced than E2.

scholarly journals Sex-dependent dysregulation of human neutrophil responses by bisphenol A

2020 ◽  
Author(s):  
Wioletta Ratajczak-Wrona ◽  
Marzena Garley ◽  
Malgorzata Rusak ◽  
Karolina Nowak ◽  
Jan Czerniecki ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Bisphenol A ◽  
Total Concentration ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Human Neutrophils ◽  
Boyden Chamber ◽  
No Production ◽  
Pathogen Elimination ◽  
Latex Beads

Abstract Background: In the present study, we aimed to investigate selected functions of human neutrophils exposed to bisphenol A (BPA) under in vitro conditions. As BPA is classified among xenoestrogens, we compared its action and effects with those of 17β-estradiol (E2).Methods: Chemotaxis of neutrophils was examined using the Boyden chamber. Their phagocytosis and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase activity were assessed via Park’s method with latex beads and Park’s test with nitroblue tetrazolium . To assess the total concentration of nitric oxide (NO), the Griess reaction was utilized. Flow cytometry was used to assess the expression of cluster of differentiation (CD) antigens. The formation of neutrophil extracellular traps (NETs) was analyzed using a microscope (IN Cell Analyzer 2200 system). Expression of the investigated proteins was determined using Western blot.Results: The analysis of results obtained for both sexes demonstrated that after exposure to BPA, the chemotactic capacity of neutrophils was reduced. In the presence of BPA, the phagocytic activity was found to be elevated in the cells obtained from women and reduced in the cells from men. Following exposure to BPA, the percentage of neutrophils with CD14 and CD284 (TLR4) expression, as well as the percentage of cells forming NETs, was increased in the cells from both sexes. The stimulatory role of BPA and E2 in the activation of NADPH oxidase was observed only in female cells. On the other hand, no influence of E2 on the expression of CD14 and CD284, chemotaxis, phagocytosis, and the amount of NET-positive neutrophils was found for both sexes. The study further showed that BPA intensified NO production and iNOS expression in the cells of both sexes. In addition, intensified expression of all tested PI3K-Akt pathway proteins was observed in male neutrophils.Conclusions: The study demonstrated the influence of BPA on neutrophil functions associated with locomotion and pathogen elimination, which in turn may disturb the immune response of these cells in both women and men. Analysis of the obtained data showed that the effect of this xenoestrogen on the human neutrophils was more pronounced than E2.

scholarly journals Sex-dependent dysregulation of human neutrophil responses by bisphenol A

2020 ◽  
Author(s):  
Wioletta Ratajczak-Wrona ◽  
Marzena Garley ◽  
Malgorzata Rusak ◽  
Karolina Nowak ◽  
Jan Czerniecki ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Bisphenol A ◽  
Total Concentration ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Human Neutrophils ◽  
Boyden Chamber ◽  
No Production ◽  
Pathogen Elimination ◽  
Latex Beads

Abstract Background: In the present study, we aimed to investigate selected functions of human neutrophils exposed to bisphenol A (BPA) under in vitro conditions. As BPA is classified among xenoestrogens, we compared its action and effects with those of 17β-estradiol (E2).Methods: Chemotaxis of neutrophils was examined using the Boyden chamber. Their phagocytosis and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase activity were assessed via Park’s method with latex beads and Park’s test with nitroblue tetrazolium . To assess the total concentration of nitric oxide (NO), the Griess reaction was utilized. Flow cytometry was used to assess the expression of cluster of differentiation (CD) antigens. The formation of neutrophil extracellular traps (NETs) was analyzed using a microscope (IN Cell Analyzer 2200 system). Expression of the investigated proteins was determined using Western blot.Results: The analysis of results obtained for both sexes demonstrated that after exposure to BPA, the chemotactic capacity of neutrophils was reduced. In the presence of BPA, the phagocytic activity was found to be elevated in the cells obtained from women and reduced in the cells from men. Following exposure to BPA, the percentage of neutrophils with CD14 and CD284 (TLR4) expression, as well as the percentage of cells forming NETs, was increased in the cells from both sexes. The stimulatory role of BPA and E2 in the activation of NADPH oxidase was observed only in female cells. On the other hand, no influence of E2 on the expression of CD14 and CD284, chemotaxis, phagocytosis, and the amount of NET-positive neutrophils was found for both sexes. The study further showed that BPA intensified NO production and iNOS expression in the cells of both sexes. In addition, intensified expression of all tested PI3K-Akt pathway proteins was observed in male neutrophils.Conclusions: The study demonstrated the influence of BPA on neutrophil functions associated with locomotion and pathogen elimination, which in turn may disturb the immune response of these cells in both women and men. Analysis of the obtained data showed that the effect of this xenoestrogen on the human neutrophils was more pronounced than E2.

The Signaling Pathways in Nitric Oxide Production by Neutrophils Exposed to N-nitrosodimethylamine

2018 ◽  
Vol 16 (2) ◽  
pp. 194-199
Author(s):  
Wioletta Ratajczak-Wrona ◽  
Ewa Jablonska
Keyword(s):  
Nitric Oxide ◽  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Polymorphonuclear Neutrophils ◽  
Microbial Pathogens ◽  
Human Neutrophils ◽  
No Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Background: Polymorphonuclear neutrophils (PMNs) play a crucial role in the innate immune system’s response to microbial pathogens through the release of reactive nitrogen species, including Nitric Oxide (NO). </P><P> Methods: In neutrophils, NO is produced by the inducible Nitric Oxide Synthase (iNOS), which is regulated by various signaling pathways and transcription factors. N-nitrosodimethylamine (NDMA), a potential human carcinogen, affects immune cells. NDMA plays a major part in the growing incidence of cancers. Thanks to the increasing knowledge on the toxicological role of NDMA, the environmental factors that condition the exposure to this compound, especially its precursors- nitrates arouse wide concern. Results: In this article, we present a detailed summary of the molecular mechanisms of NDMA’s effect on the iNOS-dependent NO production in human neutrophils. Conclusion: This research contributes to a more complete understanding of the mechanisms that explain the changes that occur during nonspecific cellular responses to NDMA toxicity.

scholarly journals The Interaction of C - Reactive Protein, Bisphenol A, & Cardiovascular Disease: A Demographical Analysis

2016 ◽  
Vol 9 (3) ◽  
pp. 63 ◽  
Author(s):  
Hassan Naji
Keyword(s):  
Cardiovascular Disease ◽  
Bisphenol A ◽  
Quantitative Research ◽  
The Body ◽  
Spearman Correlation ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
The Impact ◽  
The Relationship

<p><strong>OBJECTIVE: </strong>The main objective of the study was to investigate the role of C-reactive protein on the relationship between Bisphenol A &amp; Cardiovascular Disease, where the C-reactive protein has been taken as a moderating variable.</p><p><strong>METHODS: </strong>Quantitative research design has been incorporated for evaluating the role of C-reactive protein. Similarly, non-parametric Spearman correlation test has been conducted to assess the relationship between BPA and CVD. The data was taken out from the National Health and Nutrition Examination Survey (NHANES), which was collected in the year 2009-2010.</p><p><strong>RESULTS: </strong>The impact of urinary Bisphenol A on serum C-reactive protein was found statistically significant according to the Spearman correlation coefficient, <em>r</em>s<em>= </em>.06, <em>p </em>= .015. The scatter plots found that there is no relationship between the two variables; this observation held true after filtering the outliers from the plot.</p><p><strong>CONCLUSION:</strong> The results might have positive change by contributing to the body of knowledge on bisphenol A and by rising scientific examination of substances used by the people in the daily life. Further research to identify other possible causes of CVD and elevation of CRP is recommended.</p>

scholarly journals RESPIRATORY SUPPORT AT PREHOSPITAL STAGE OF EMERGENCY MEDICAL CARE: CURRENT STATUS, PROBLEMS AND PROSPECTS OF DEVELOPMENT

2018 ◽  
Vol 18 (4) ◽  
pp. 53-59
Author(s):  
A. L. Ershov
Keyword(s):  
Respiratory Distress ◽  
Medical Care ◽  
Patient Outcomes ◽  
Current Status ◽  
Emergency Medical Care ◽  
Study Objective ◽  
Emergency Medical ◽  
The Impact ◽  
Subsequent Patient

Patients with respiratory distress often seek emergency medical care and are frequently transported by emergency medical services (EMS). Some of them are in need of urgent respiratory support.EMSencounters with patients in respiratory distress and the role of mechanical ventilation (MV) in their treatment have not been well documented. The study objective was to characterize the epidemiology of prehospital respiratory distress and the impact of MV at subsequent patient outcomes. Some of the new trends in prehospital respiratory therapy have also been described.

The effect of ethanol and N-nitrosodimethylamine on the iNOS-dependent NO production in human neutrophils. Role of NF-κB

Xenobiotica ◽  
2017 ◽  
Vol 48 (5) ◽  
pp. 498-505 ◽  
Author(s):  
Karolina Nowak ◽  
Wioletta Ratajczak-Wrona ◽  
Marzena Garley ◽  
Ewa Jabłońska
Keyword(s):  
Human Neutrophils ◽  
No Production

scholarly journals Characterization of CD38 in the major cell types of the heart: endothelial cells highly express CD38 with activation by hypoxia-reoxygenation triggering NAD(P)H depletion

2018 ◽  
Vol 314 (3) ◽  
pp. C297-C309 ◽  
Author(s):  
James Boslett ◽  
Craig Hemann ◽  
Fedias L. Christofi ◽  
Jay L. Zweier
Keyword(s):  
Endothelial Cells ◽  
Endothelial Dysfunction ◽  
Cardiac Myocytes ◽  
Cell Types ◽  
No Production ◽  
Imaging Method ◽  
Enos Uncoupling ◽  
The Impact ◽  
Hypoxia Reoxygenation

The NAD(P)+-hydrolyzing enzyme CD38 is activated in the heart during the process of ischemia and reperfusion, triggering NAD(P)(H) depletion. However, the presence and role of CD38 in the major cell types of the heart are unknown. Therefore, we characterize the presence and function of CD38 in cardiac myocytes, endothelial cells, and fibroblasts. To comprehensively evaluate CD38 in these cells, we measured gene transcription via mRNA, as well as protein expression and enzymatic activity. Endothelial cells strongly expressed CD38, while only low expression was present in cardiac myocytes with intermediate levels in fibroblasts. In view of this high level expression in endothelial cells and the proposed role of CD38 in the pathogenesis of endothelial dysfunction, endothelial cells were subjected to hypoxia-reoxygenation to characterize the effect of this stress on CD38 expression and activity. An activity-based CD38 imaging method and CD38 activity assays were used to characterize CD38 activity in normoxic and hypoxic-reoxygenated endothelial cells, with marked CD38 activation seen following hypoxia-reoxygenation. To test the impact of hypoxia-reoxygenation-induced CD38 activation on endothelial cells, NAD(P)(H) levels and endothelial nitric oxide synthase (eNOS)-derived NO production were measured. Marked NADP(H) depletion with loss of NO and increase in superoxide production occurred following hypoxia-reoxygenation that was prevented by CD38 inhibition or knockdown. Thus, endothelial cells have high expression of CD38 which is activated by hypoxia-reoxygenation triggering CD38-mediated NADP(H) depletion with loss of eNOS-mediated NO generation and increased eNOS uncoupling. This demonstrates the importance of CD38 in the endothelium and explains the basis by which CD38 triggers post-ischemic endothelial dysfunction.

scholarly journals The role of patient care workers in private hospitals in the Cape Metropole, South Africa

Curationis ◽  
2017 ◽  
Vol 40 (1) ◽  
Author(s):  
Louise A. Aylward ◽  
Talitha Crowley ◽  
Ethelwynn L. Stellenberg
Keyword(s):  
Patient Care ◽  
Support Staff ◽  
Direct Patient Care ◽  
Private Hospitals ◽  
Direct Supervision ◽  
Nursing Support ◽  
Study Objective ◽  
Care Workers ◽  
The Impact

Background: Nursing managers have to meet expectations of patients despite economic pressures, an increasing burden of disease and nursing shortages. Shifting health care-related tasks to lower categories of staff, including non-nursing support staff, has become one solution to address this dilemma. Patient care workers are a specific group of non-nursing support staff working in South African hospitals. Although patient care workers have been used for several years and their numbers are increasing, there are controversial opinions about the role of patient care workers, ranging from praise for their contribution towards patient care to serious concerns about the impact of their role on patient safety.Objective: The study objective was to explore and describe the role of patient care workers in private hospitals.Methods: A qualitative, descriptive design was applied to explore the role of patient care workers. Purposive sampling was used to select unit managers, nurses and patient care workers from medical and surgical wards of three private hospitals. Fifteen semi-structured interviews were conducted and transcribed verbatim. The researcher applied interpretative data analysis to move from the participants’ descriptions of their experiences to a synthesis of all participants’ descriptions.Results: Patient care workers are involved in direct patient care and spend much time with patients, often not working under direct supervision of registered nurses despite limited training and lack of regulation. Their contribution, however, is valued by nurses.Conclusion: Patient care workers are well-integrated into the patient care team and are mostly seen as nurses. Yet, there are concerns about their evolving role despite their limited training and the lack of direct supervision. Regulating the work of patient care workers is recommended.

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