scholarly journals Assessment of the Effects of Newly Fabricated CaO, CuO, ZnO Nanoparticles on Callus Formation Maintainance of Alfalfa (Medicago Sativa L.) Under In Vitro Salt Stress

2021 ◽  
Author(s):  
Merve Simsek ◽  
Busra Yazicilar ◽  
Fatma Boke ◽  
Hayrunisa Nadaroglu ◽  
Azize Alayli ◽  
...  
Keyword(s):  
Salt Stress ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Nacl Stress ◽  
The Other ◽  
Antioxidant Enzyme Activities ◽  
Zno Nps ◽  
Induction Stage

Abstract Nanoparticules plays an important role in plant adaptation to abiotic stress, especially in response to salt stress. In this study, two alfalfa lines (Erzurum, and Muş) were used as the material for the response NaCl to CuO, ZnO and CaO nanoparticules (NPs). CaO is evident to be higher effective than CuO, ZnO in callus induction from leaf explants. The antioxidant enzyme activities were also determined in the callus cultures. The maximum activity in MDA analysis was observed from callus treated of 50 mM NaCl with 0.8 ppm CuO NPs. The callus induction stage without salt treatments indicated a best result in 0.8 ppm CaO NPs for H2O2 value compared to the other NPs. The callus induction stage without salt treatments indicated a best result in 0.8 ppm CaO NPs for POD value compared to the other NPs for POD activity. The best response in protein rate was obtained from callus induction stage and callus formation stage after 50 mM treatment NaCl with 0.8 ppm CuO. LSCM analysis evident that the NPs could migitate the negative effects of NaCl stress by the elimination of stress severity in callus cells. SEM analysis was supported the results obtained by LSCM analysis. Our findings suggest that CuO, CaO and ZnO NPs can offer a simple and effective method to protect alfalfa callus from NaCl stress severity.

scholarly journals Callus Induction in Baru (Dipteryx alata Vog.) Explants

2018 ◽  
Vol 47 (2) ◽  
pp. 538-543
Author(s):  
Rodrigo Kelson S. REZENDE ◽  
Ana Maria N. SCOTON ◽  
Maílson V. JESUS ◽  
Zeva V. PEREIRA ◽  
Fernanda PINTO
Keyword(s):  
Ascorbic Acid ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Alternative Technique ◽  
Propagation Methods ◽  
Dipteryx Alata

Baru (Dipteryx alata Vog.) is a species with great economic and environmental potential; it has popular acceptance, besides being a very productive species. Alternative propagation methods are important for species maintenance and exploration. Thus, micropropagation emerged as an alternative technique, providing genetic stability and the production of a large number of seedlings. The aim of the present investigation was to develop a callus induction protocol for in vitro baru explants. The tested explants were nodal, internodal and foliar segments. The explants were disinfected for 30 seconds in 70% alcohol (v/v) and 2 minutes in sodium hypochlorite (1.25% active chlorine). This was followed by triple washing. The inoculation was carried out in test tubes containing 15 mL MS medium (30 g L-1 sucrose, 6 g L-1 agar and 100 mg L-1 ascorbic acid) supplemented with 2.0 mg L-1 naphthalene acetic acid (NAA). The solution also contained 0.0, 2.5 or 5.0 mg L-1 of 6-benzylaminopurine (BAP) with the pH adjusted to 5.8. In the incubation phase, the explants were cultured for seven days in the dark and then subjected to a photoperiod of 16 hours (43 µmol m-2 s-1) at 25 ± 2 °C. The treatments were studied with 2.5, 5.0, 7.5 or 10.0 mg L-1 BAP additions to the MS. Callus formation, contamination and oxidation evaluations were undertaken. The results obtained when using 2.0 mg L-1 NAA concluded that such a treatment should be used to induce callogenesis from nodal explants, while for the tested baru leaf explants, the best results for callus formation were given by the combination of 2.0 mg L-1 NAA with 2.5 mg L-1 of BAP to.

scholarly journals Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

2015 ◽  
Vol 49 (4) ◽  
pp. 199-204 ◽  
Author(s):  
S Mahmud ◽  
S Akter ◽  
IA Jahan ◽  
S Khan ◽  
A Khaleque ◽  
...  
Keyword(s):  
Retention Time ◽  
Callus Tissue ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Poor Performance ◽  
The Other ◽  
Ms Medium ◽  
Green Callus

A protocol was developed to produce large amount of callus in short a period of time from leaf explants of Stevia rebaudiana Bert. The highest amount of white callus was obtained on MS medium supplemented with 2.5 mg/l 2, 4-D and 0.5 mg/l BAP after 3 weeks of inoculating leaf segments. On the other hand, 0.5 mg/l BAP and 1.0 mg/l Kn exhibits poor performance towards callus formation while after using 1.0 mg/l Kn alone did not develop any callus. In this experiment, highest amount of green callus was obtained when MS medium supplemented with 2.5 mg/l NAA and 10% coconut water was used. An improved analytical method HPLC was applied to analyze stevioside extracted from the leaf and callus of Stevia rebaudiana. The stevioside in each sample were analyzed by comparing their retention times with those of the standards. The retention time (RT) of stevioside for leaves were found 14.96 and for callus 13.81 mins. The percentage of stevioside content from leaves and callus was 12.19% and 12.62% respectively DOI: http://dx.doi.org/10.3329/bjsir.v49i4.22621 Bangladesh J. Sci. Ind. Res. 49(4), 199-204, 2014

scholarly journals In vitro cultivation and regeneration of Solanum melongena (L.) using stem, root and leaf explants

1970 ◽  
Vol 1 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Bishnu Pada Ray ◽  
Lutful Hassan ◽  
Smreeti Kana Sarker
Keyword(s):  
Key Words ◽  
Callus Induction ◽  
Callus Formation ◽  
Solanum Melongena ◽  
Leaf Explants ◽  
In Vitro Cultivation ◽  
Ms Medium

The treatment combinations was BAP (0, 2.0, 3.0 and 4.0 mg/L) and NAA (0, 0.1, 0.5, and 1.0 mg/L). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem and 8.2 days required for callus induction. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. Key words: Regeneration; BAP; NAA. Nepal Journal of Biotechnology. Jan. 2011, Vol. 1, No. 1 : 49-54

scholarly journals In Vitro plant regeneration from leaf explants of Tagetes erecta L.

2021 ◽  
Vol 56 (2) ◽  
pp. 69-74
Author(s):  
JL Munshi ◽  
R Baksha ◽  
MZ Rahaman ◽  
NN Huque ◽  
EA Zinat ◽  
...  
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Leaf Shape ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Tagetes Erecta ◽  
Ms Medium ◽  
Shoot Bud ◽  
Tagetes Erecta L

Regeneration of multiple shoots via callus induction and organogenesis was obtained from young leaf explants of the field grown marigold (Tagetes erecta L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (90%) of callus formation was observed within two weeks on MS medium supplemented with 5.0 mg/l BAP with 2.5 mg/l NAA. The maximum percentage (80%) of shoot bud formation (10±0.5/callus) was obtained from MS medium containing 1.0 mg/l BAP with 0.5 mg/l kinetin. The regenerated shoots developed highest percentages (90%) of roots on half strength MS medium supplemented with 1.0 mg/l IBA. The plantlets when transferred into potsoil 80% survived. Regenerated plants were morphologically uniform with normal leaf shape and growth pattern. Bangladesh J. Sci. Ind. Res.56(2), 69-74, 2021

scholarly journals Callus formation and organogenesis in tissue culture Deschampsia antarctica E. Desv.

2019 ◽  
Vol 17 (1) ◽  
pp. 8-15
Author(s):  
I. I. Konvalyuk ◽  
L. P. Mozhylevs’ka ◽  
V. A. Kunakh
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Shoot Growth ◽  
Callus Formation ◽  
Leaf Explants ◽  
Deschampsia Antarctica ◽  
Donor Plant ◽  
Growth Point ◽  
The Media

Aim. The aim of the work was to determine the optimal conditions for induction and proliferation of tissue culture obtained from D. antarctica plants from various localities of the Maritime Antarctica. Methods. Tissue and organ culture techniques. Results. The media В5 supplemented with 2 mg/l 2,4-D + 0,1 mg/l BAP, В5 supplemented with 10 mg/l 2,4-D + 0,2 mg/l BAP and МС, supplemented with 5 mg/l 2,4-D + 0,1 mg/l Kin were optimal for callus induction from different types of explants. The media with a reduced concentrations of auxins and cytokinins were the most effective for maintenance of continuous tissue culture compared to the media for callus induction: B5 + 2 mg/l 2,4-D mg/l + 0,1 mg/l BAP and MC + 1 mg/l 2,4-D + 0.1 mg/l Kin. Tissues from shoot growth point and leaf explants of genotypes DAR12a and G/D12-2a on medium B5 with 2 mg/l 2,4-D + 0.1 mg/l BAP and B5 with 10 mg/l 2,4-D + 0,2 mg/l BAP demonstrated the ability to spontaneous organogenesis and formed separate shoots. Conclusions. Conditions have been determined for the induction and proliferation of tissue culture from leaf, root, and shoot growth point explants of D. antarctica. The frequency of callus formation depended on the mineral composition of medium, ratios and concentrations of growth regulators, type of explant, and genotype of a donor-plant. As a result of spontaneous organogenesis, regenerated plants were obtained, conditions for their rooting in vitro were elaborated. The proposed methods for induction and proliferation tissue culture of D. antarctica in vitro can be used to produce the plant material useful for a various investigations. Keywords: Deschampsia antarctica E. Desv., tissue culture, organogenesis in vitro, frequency of callogenesis.

FACTORS AFFECTING THE REGENERATION OF PEPPER (CAPSICUM ANNUUM L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1120G-1120
Author(s):  
J. L. Jacobs ◽  
C. T. Stephens
Keyword(s):  
Growth Hormone ◽  
Silver Nitrate ◽  
Callus Formation ◽  
Leaf Explants ◽  
Regeneration System ◽  
Factors Affecting ◽  
Nitrate Concentrations ◽  
Leaf Differentiation ◽  
Whole Plants

Several growth hormone combinations and silver nitrate concentrations were examined for their effect on regeneration of different pepper genotypes. Primary leaf explants from in vitro seedlings were cultured on a revised Murashige and Skoog medium supplemented with auxin, cytokinin and 1.6% glucose. Combinations of different concentrations of indole-3-acetic acid (IAA), 0-5 mg/l, and 6-benzylaminopurine (BAP), 0-5 mg/l, were tested to determine the most effective medium for shoot primordium formation. Experiments with IAA and BAP did not result in a specific growth hormone combination appropriate for regeneration of all genotypes tested. Of the silver nitrate concentrations tested, 10 mg/l resulted in the best shoot and leaf differentiation and reduced callus formation. Differences in organogenic response of individual genotypes were evaluated on a single regeneration medium. Whole plants were regenerated from 11 of 63 genotypes examined. Based on these experiments, a reproducible regeneration system for pepper was developed with a total of 500 plants regenerated to date.

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

scholarly journals Induction and growth pattern of callus from Piper permucronatum leaves

2016 ◽  
Vol 18 (1) ◽  
pp. 142-148 ◽  
Author(s):  
M.R.A. SANTOS ◽  
M.C.M. GUIMARÃES ◽  
E.S. PAZ ◽  
G.M.O. MAGALHÃES ◽  
C.A. SOUZA ◽  
...  
Keyword(s):  
Growth Pattern ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Callus Growth ◽  
Deceleration Phase ◽  
Stomach Pain ◽  
Perennial Shrub ◽  
A Cell

ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals In vitro propagation of six selected sugarcane mutant clones through leaf explants

2021 ◽  
Vol 883 (1) ◽  
pp. 012075
Author(s):  
R Purnamaningsih ◽  
D Sukmadjaja ◽  
S Suhesti ◽  
S Rahayu
Keyword(s):  
Callus Induction ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
Culture Techniques ◽  
High Productivity ◽  
Media Formulation ◽  
Number Of Shoots

Abstract Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.

Sign in / Sign up

Export Citation Format

Share Document