MicroRNA-124 alone might represent an indicator signifying cholangiocarcinoma prognosis

Abstract Background: Previous studies have demonstrated that microRNAs (miRNAs) played a crucial role in various diseases, including cancers. The aim of the study was to evaluate the clinical significance of miR-124 in patients with cholangiocarcinoma (CCA).Methods: The expression pattern of miR-124 was detected in CCA tissues using quantitative reserve transcription polymerase chain reaction (qRT-PCR). The correlation of miR-124 expression with clinicopathological features and overall survival of patients were explored using chi-square test, Kaplan-Meier methods and Cox regression analyses.Results: The miR-124 expression level was strong down-regulated in CCA tissues compared with normal para-cancerous tissues (P<0.001). Moreover, aberrant miR-124 expression was significantly associated with differentiation (P=0.045) and lymph node metastasis (P=0.040). In addition, Kaplan-Meier method and log-rank test revealed that patients with low miR-124 expression has a poorer overall survival compared with those with high miR-124 expression (P=0.002). Furthermore, multivariate analysis confirmed that miR-124 expression (P=0.006; HR=2.006; 95%CI: 1.224-3.289) was an independent prognostic indicator in CCA.Conclusions: Collectively, our results defined miR-124 expression plays important roles in CCA patients. MiR-124 expression might used as a valuable prognostic biomarker for patients with CCA.

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Survival Outcomes for Induction vs Adjuvant Chemotherapy in Squamous Cell Carcinoma of the Maxillary Sinus

Otolaryngology ◽

10.1177/0194599818804777 ◽

2018 ◽

Vol 160 (4) ◽

pp. 658-663 ◽

Cited By ~ 4

Author(s):

Phoebe Kuo ◽

Sina J. Torabi ◽

Dennis Kraus ◽

Benjamin L. Judson

Keyword(s):

Overall Survival ◽

Adjuvant Chemotherapy ◽

Maxillary Sinus ◽

Induction Chemotherapy ◽

Squamous Cell ◽

Cox Regression ◽

Rank Test ◽

Controlled Clinical Trial ◽

Log Rank Test ◽

Kaplan Meier

Objective In advanced maxillary sinus cancers treated with surgery and radiotherapy, poor local control rates and the potential for organ preservation have prompted interest in the use of systemic therapy. Our objective was to present outcomes for induction compared to adjuvant chemotherapy in the maxillary sinus. Study Design Secondary database analysis. Setting National Cancer Database (NCDB). Subjects and Methods In total, 218 cases of squamous cell maxillary sinus cancer treated with surgery, radiation, and chemotherapy between 2004 and 2012 were identified from the NCDB and stratified into induction chemotherapy and adjuvant chemotherapy cohorts. Univariate Kaplan-Meier analyses were compared by log-rank test, and multivariate Cox regression was performed to evaluate overall survival when adjusting for other prognostic factors. Propensity score matching was also used for further comparison. Results Twenty-three patients received induction chemotherapy (10.6%) and 195 adjuvant chemotherapy (89.4%). The log-rank test comparing induction to adjuvant chemotherapy was not significant ( P = .076). In multivariate Cox regression when adjusting for age, sex, race, comorbidity, grade, insurance, and T/N stage, there was a significant mortality hazard ratio of 2.305 for adjuvant relative to induction chemotherapy (confidence interval, 1.076-4.937; P = .032). Conclusion Induction chemotherapy was associated with improved overall survival in comparison to adjuvant chemotherapy in a relatively small cohort of patients (in whom treatment choice cannot be characterized), suggesting that this question warrants further investigation in a controlled clinical trial before any recommendations are made.

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The relationship between RECK Gene Polymorphisms and the prognosis of cholangiocarcinoma

10.21203/rs.3.rs-44943/v1 ◽

2020 ◽

Author(s):

Ning Wang ◽

Yanni Li ◽

Yanfang Zheng ◽

Huoming Chen ◽

Xiaolong Wen ◽

...

Keyword(s):

Overall Survival ◽

Survival Rate ◽

Cox Regression ◽

Univariate Analysis ◽

Overall Survival Rate ◽

Chi Square ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Chi Square Test ◽

The Relationship

Abstract Background The study was designed to examine the reversion inducing cysteine rich protein with Kazal motifs (RECK) levels in patients with cholangiocarcinoma (CCA) and assess its role in CCA prognosis. Methods Quantitative real-time PCR (qRT-PCR) was used to determine the expression of RECK mRNA in 127 pairs of CCA samples and controls. Chi-square test was conducted to analyze the effects of clinical features on RECK expression. Kaplan-Meier curves were plotted to determine the overall survival rate of CCA patients with different RECK expression. The prognostic biomarkers for CCA patients were identified using the Cox regression analysis. Results Significantly down-regulated expression of RECK mRNA was determined in CCA tissues compared to noncancerous controls (P < 0.05). Chi-square test suggested reduced RECK expression was related with invasion depth (P = 0.026), differentiation (P = 0.025), lymphatic metastasis (P = 0.010) and TNM stage (P = 0.015). However, age, sex, tumor size and family history had no significant links with RECK expression (all, P > 0.05). The survival curves showed that patients with low RECK expression had a shorter overall survival rate than those with high RECK expression. Both the univariate analysis (P = 0.000, HR = 5.290, 95%CI = 3.195–8.758) and multivariate analysis (P = 0.000, HR = 5.376, 95%CI = 2.231–8.946) demonstrated that RECK was an independent biomarker for predicting the outcomes of CCA patients. Conclusions Taken together, the expression of RECK was down-regulated in CCA and it might be an efficient biomarker for CCA patients.

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Ifosfamide (IFO) and doxorubicin (DOX) dose-intensities seem related to overall survival in adult soft-tissue sarcoma (STS) patients

Journal of Clinical Oncology ◽

10.1200/jco.2006.24.18_suppl.9581 ◽

2006 ◽

Vol 24 (18_suppl) ◽

pp. 9581-9581

Author(s):

G. F. Almeida ◽

G. Castro ◽

I. M. Snitcovsky ◽

A. C. Bassani ◽

M. E. Diz ◽

...

Keyword(s):

Overall Survival ◽

Tumor Size ◽

Rank Test ◽

Lower Grade ◽

Chi Square ◽

Outpatient Unit ◽

Kaplan Meier ◽

Chi Square Test ◽

The Relationship ◽

Palliative Setting

9581 Background: IFO/DOX dose intensities (DI) seem to impact on the outcome of STS. We explored retrospectively the relationship between DI and overall survival (OS) in STS. Methods: From Jan/00 to Jun/05, 70 untreated STS pts received IFO/DOX, 32 as neo/adjuvant and 38 in the palliative setting at our outpatient unit. Filgrastin was not mandatory. Median age 47 y (17–74 y), 44 male; mean tumor size 13.6 cm in the neo/adjuvant and 16.5 cm in the palliative group (p=0.202, t-test). Most frequent histologies: leiomyo (16 pts), synovial (13), malignant fibrous histiocytoma (8) and liposarcoma (8). 28 pts had lower/ 9 upper limb tumors, 9 retroperitoneal, 9 trunk, 6 mediastinal, 5 visceral and 4 head and neck. Kaplan-Meier survival curves were considered from diagnosis and compared by log-rank test. Results: For the 70 pts, the mean DI for IFO and DOX were 2.5±0.9 mg/m2/wk and 18.8±6.0 mg/m2/wk, respectively. There was no difference between neo/adjuvant and palliative IFO/DOX DI (p=0.314/p=0.247, respectively). With 19-mo median f-up, the median OS (mOS) was 43 mo in the neo/adjuvant group with an advantage for pts submitted to conservative surgeries (46.5 mo vs. 16.8 mo; HR 0.185, 95%CI 0.003–0.399, p=0.007) as well as in those diagnosed with tumors with less than 3 mitoses/10 HPF (48.3 mo vs. 18.8 mo; HR 0.272, 95%CI 0.058–0.871, p=0.031). No differences in mOS related to tumor size, margin status or primary sites were found. According to IFO DI, the mOS were 46.5 mo, not reached (NR), 14.5 mo and 43 mo for pts in the 1st and subsequent DI quartiles (chi-square test for trend, p=0.004). In the median f-up of 9.8 mo, pts in the palliative setting presented mOS 21.8 mo, superior in the lower grade subgroup (NR vs. 11.1 mo; HR 0.130, 95%CI 0.076–0.746, p=0.014) and in the STS not from extremities (40.9 mo vs. 10.8 mo; HR 2.152, 95%CI 0.959–5.137, p=0.063). According to IFO DI quartiles, we also found a direct correlation between mOS (11.3 mo, 19 mo, 45.1 mo, and NR) and DI (p=0.052), and similar trend was shown for DOX DI, with 11.3 mo, 10.3 mo, NR, and 40.9 mo mOS for the 1st, 2nd, 3rd and 4th quartiles (p=0.018). Conclusions: In these STS adult pts, we have found a relationship between IFO and DOX DI and OS. Further evaluations of more intensive chemotherapy schedules are warranted. No significant financial relationships to disclose.

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Development of predictive prognostic nomogram for NECs of rectum on population-based exploration

Endocrine Connections ◽

10.1530/ec-18-0353 ◽

2018 ◽

Vol 7 (11) ◽

pp. 1178-1185 ◽

Cited By ~ 1

Author(s):

Yang Lv ◽

Ning Pu ◽

Wei-lin Mao ◽

Wen-qi Chen ◽

Huan-yu Wang ◽

...

Keyword(s):

Cox Regression ◽

Population Based ◽

Prognostic Indicators ◽

Rank Test ◽

Chi Square ◽

Related Factors ◽

Ki 67 ◽

Cox Regression Analysis ◽

Kaplan Meier ◽

Chi Square Test

Aim We aim to investigate the clinical characteristics of the rectal NECs and the prognosis-related factors and construct a nomogram for prognosis prediction. Methods The data of 41 patients and 1028 patients with rectal NEC were retrieved respectively from our institution and SEER database. OS or PFS was defined as the major study outcome. Variables were compared by chi-square test and t-test when appropriate. Kaplan–Meier analysis with log-rank test was used for survival analysis and the Cox regression analysis was applied. The nomogram integrating risk factors for predicting OS was constructed by R to achieve superior discriminatory ability. Predictive utility of the nomogram was determined by concordance index (C-index) and calibration curve. Results In the univariate and multivariate analyses, tumor differentiation, N stage, M stage and resection of primary site were identified as independent prognostic indicators. The linear regression relationship was found between the value of Ki-67 index and the duration of OS (P < 0.05). Furthermore, the independent prognostic factors were added to formulate prognostic nomogram. The constructed nomogram showed good performance according to the C-index. Conclusions Contrary to WHO classification guideline, we found that the rectal NEC diseases are heterogeneous and should be divided as different categories according to the pathological differentiation. Besides, the nomogram formulated in this study showed excellent discriminative capability to predict OS for those patients. More advanced predictive model for this disease is required to assist risk stratification via the formulated nomogram.

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Clinicopathologic and Survival Characteristics of patients with squamous cell carcinoma and adenocarcinoma of uterine cervix in Yazd, Iran

Chinese Journal of Medical Research ◽

10.37515/cjmr.091x.3205 ◽

2020 ◽

Vol 3 (2) ◽

pp. 57-61

Author(s):

Fariba Binesh ◽

◽

Sanaz Azadi ◽

Ali Akhavan ◽

Tahmine Hashemi Zade ◽

...

Keyword(s):

Overall Survival ◽

Squamous Cell ◽

Cox Regression ◽

Cell Cancer ◽

Chi Square ◽

Historical Cohort ◽

Factors Affecting ◽

Frequency Table ◽

Kaplan Meier ◽

Chi Square Test

Introduction: Though the incidence of cervical squamous cell cancer (SCC) has reduced during recent years, the amount of cervical adenocarcinoma (AC) has propagated. There is a controversy over whether prognosis is better in SCC or AC. Similar studies have not been conducted in Iran. Material and methods: This is a descriptive-analytic study that is based on historical cohort method. In this retrospective work, all cases of cancer patients were studied from 2004 to 2015 and the medical records of all women recognized with cervical SCC and AC treated in Shahid Sadoughi teaching hospital and Shahid Ramesanzadeh Radiotherapy Center were recovered. In these patients, the epidemiologic characteristics, survival and the factors affecting the survival were investigated. Statistical analysis included frequency table and Chi-Square test. Patient survival was assessed using Kaplan- Meier assessments, and multivariate analysis was done by the Cox regression mode. Results: This study was done on 158 patients identified with cervical carcinoma. Their mean age at the time of diagnosis was 53.3987±1.02150 years. According to histopathologic types, 132 of the patients were classified as SCC with mean age of 52.4840±1.10612 years; while 26 patients were identified as AC with mean age of 58.0385±2.49830 years. The overall survival was 96.338±4.434 months (95% confidence interval) and it was 100.459±4.342 and 54.475±5.334 months for SCC and AC respectively. In the early and advanced stages, overall survival of patients with SCC was different (p=0.001). It was true about the patients with AC (p=0.002). Conclusion: The results of the current study showed the prognosis is worse in patients with cervical AC than cervical SCC

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The expression of HOXA9 and its prognostic value in cervical cancer

10.21203/rs.3.rs-54158/v1 ◽

2020 ◽

Author(s):

Keqian Zhang ◽

Tianqi Mao ◽

Zhicheng He ◽

Xiaojiao Wu ◽

Yu Peng ◽

...

Keyword(s):

Cervical Cancer ◽

Prognostic Value ◽

Cox Regression ◽

Figo Stage ◽

Rank Test ◽

Chi Square ◽

Cox Regression Analysis ◽

Normal Tissues ◽

Kaplan Meier ◽

Chi Square Test

Abstract Background The HOXA9 gene, belonging to homeobox (HOX) gene family, has been recently reported dys-expressed in several kinds of human cancers. This study aimed to investigate the expression of HOXA9 and its prognostic value in cervical cancer. Methods The HOXA9 mRNA expression was detected with a quantitative real-time polymerase chain reaction (qRT-PCR) assay, and the association of HOXA9 expression with clinical characteristic was analyzed via chi-square test. Kaplan-Meier and cox regression analyses were conducted to estimate the prognostic value of HOXA9 in cervical cancer. Results HOXA9 expression was significantly down-expressed in cervical cancer tissues compared with that in adjacent normal tissues (P < 0.01). And the expression of HOXA9 was significantly associated with TNM stage, pathological grade, FIGO stage and differentiation (All P < 0.05). In addition, Kaplan–Meier analysis indicated that the overall survival of patients with low HOXA9 expression was shorter than those with high HOXA9 expression (log rank test, P = 0.000). Cox regression analysis revealed that HOXA9 had a high prognostic value in cervical cancer. Conclusion HOXA9 is down-regulated and involved in the development of cervical cancer. Moreover, it may be an useful independent prognostic bio-marker for patients with cervical cancer.

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Rab25 Acts as a Promising Novel Bio-Marker in the Prognosis of Patients with Hepatocellular Carcinoma

10.21203/rs.3.rs-43510/v1 ◽

2020 ◽

Author(s):

Zhong Dai ◽

Ke-Qing Yao ◽

Xing-Sheng Hu ◽

Yi-Qun Li ◽

Yu-Tao Liu ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Prognostic Value ◽

Cox Regression ◽

Rank Test ◽

Chi Square ◽

Cox Regression Analysis ◽

Normal Tissues ◽

Kaplan Meier ◽

Chi Square Test ◽

The Relationship

Abstract Background: Rab25 was indicated to be involved in several human tumors. However, the clinical significance of Rab25 in hepatocellular carcinoma (HCC) was still unclear. The purpose of this study was to investigate the expression and prognostic value of Rab25 in HCC.Methods: The relative mRNA expression levels of Rab25 in HCC tissues and adjacent normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was used to analyze the relationship between Rab25 expression and clinical characteristics of patients. The prognostic value of Rab25 in HCC was estimated through Kaplan-Meier method and cox regression analysis.Results: Rab25 gene expression level was significantly higher in HCC tissues than that in normal tissues (P<0.001). Importantly, the increased Rab25 expression was closely associated with TNM stage (P=0.024), metastasis (P=0.022) and invasion classification (P=0.039). Moreover, patients with high Rab25 expression tended to have obviously shorter overall survival than those with low expression of Rab25 (log rank test, P<0.001) via Kaplan-Meier analysis. Univariate and multivariate cox regression analyses revealed that Rab25 was an independent prognostic factor of HCC.Conclusions: Rab25 is up-regulated in HCC and contributes to the progression of this tumor. What’s more, Rab25 may be a potential bio-marker for the prognosis of HCC.

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Analysis of lymphocyte-neutrophil relationship in patients with advanced kidney cancer and the response to one-line treatment with target therapies.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e23034 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e23034-e23034

Author(s):

Luciana Paola Acosta ◽

Martin Eduardo Richardet ◽

Matias Molina ◽

Eduardo Richardet

Keyword(s):

Prognostic Factors ◽

Kidney Cancer ◽

Prognostic Indicator ◽

Smoking Habits ◽

Rank Test ◽

Line Treatment ◽

Chi Square ◽

Kaplan Meier ◽

Chi Square Test ◽

Medical Histories

e23034 Background: In kidney cancer, some important prognostic factors of survival are known and inflammation plays a key role. It has been proven that cancer progress is not only determined by the characteristics of the tumor but also by the response of the host. It is known that a high RNL has been identified as an independent prognostic factor associated to poor survival in several/different types of cancer, including breast cancer, colon cancer, gastric cancer, mesothelioma and pancreas. The primary aim was to evaluate if RNL can be used as a prognostic indicator of SLP in patients with advanced CCR with 1st line treatment with tirosin-kinasa inhibitors. The secondary aim was to evaluate whether there was a relationship between prognostic factors, smoking habits, BMI, anemia, calcemia, KPS and nephrectomized patients as opposed to those who were not operated with RNL and SLP. Methods: Retrospective and analytical study. The medical histories from patients with metastatic kidney cancer diagnosis were analyzed as well as those who were undergoing treatment with Sunitinib and Pazopanib. The cut value of > ó < 3 was taken as a reference point for RNL. Overall survival analysis will be evaluated through the Kaplan-Meier curve and the meaning will be verified by the log-rank test. The multivariate analysis will be done through the Chi-square test. Results: A total of 65 patients were included in this study. 50 patients (75.38%) received treatment with Sunitinib and 15 patients (24.62%) with Pazopanib. It was observed that RNL < 3 was correlated with a higher SLP 21,6 months vs 8.9 months, p = 0,00002 and it was statistically significant. In subgroup analysis, those patients with normal values of corrected calcium the survival was 20 months vs 12, 2 months (p: 0.01), non-smokers 21 months vs 12 months (p: 0.05), KPS > 1 17,72 months Vs 8,8 months (p: 0.03) and a RNL < 3 showed a higher survival in a statistically significant way. There were no differences in survival when we performed the anemia analysis, BMI, smoking habits and KPS 0 to 1. Conclusions: We can conclude that those patients with RNL < 3 and who had already undergone treatment with ITK revealed a better SLP in a statistically significant way.

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Prognostic value of piR-39980 in patients with colorectal cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2020.38.15_suppl.e16087 ◽

2020 ◽

Vol 38 (15_suppl) ◽

pp. e16087-e16087

Author(s):

Wei Peng ◽

Jingfeng Mei ◽

Jianwei Lu ◽

Jun Bao ◽

Guoren Zhou

Keyword(s):

Colorectal Cancer ◽

Overall Survival ◽

Prognostic Value ◽

Cox Regression ◽

Survival Curve ◽

Prognostic Significance ◽

Rank Test ◽

Chi Square ◽

Log Rank Test ◽

Independent Indicator

e16087 Background: Colorectal cancer (CRC) accounts for a common gastrointestinal malignancy all over world. Piwi-interacting RNAs (piRNAs) show a substantial role in the oncogenesis of a variety of tumors. The objective of this work was to uncover the expression profile of piR-39980 and its prognostic value in CRC. Methods: The levels of piR-39980 expression in CRC tissues and paired normal tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Association of piR-39980 with CRC clinical feathers was assessed by Chi-square test. Overall survival curve was built via log-rank test by Kaplan–Meier analysis. The prognostic significance of piR-39980 in CRC was measured by Cox regression model. Results: piR-39980 was upregulated in CRC specimens than the paired normal specimens ( P < 0.001). Importantly, upregulation of piR-39980 was related to tumor size and T stage (all P < 0.05). Survival evaluation suggested that CRC folks with high expression of piR-39980 went through poorer overall survival than folks with low piR-39980 expression (log rank test, P = 0.0429). piR-39980 could be an independent indicator for CRC patients’ prognosis (HR = 3.308, 95% CI = 1.762-6.594, P = 0.043). Conclusions: piR-39980 plays oncogenic roles in CRC tumorigenesis and may be an independent indicator for CRC prognosis.

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EEF1A Acts as A Promising Novel Bio-Marker in the Prognosis of Patients With Cholangiocarcinoma

10.21203/rs.3.rs-53241/v1 ◽

2020 ◽

Author(s):

Ning Wang ◽

Yanni Li ◽

Yanfang Zheng ◽

Huoming Chen ◽

Xiaolong Wen ◽

...

Keyword(s):

Cox Regression ◽

Predictive Biomarker ◽

Regression Analyses ◽

Chain Reaction ◽

Chi Square ◽

Qrt Pcr ◽

Node Metastasis ◽

Kaplan Meier ◽

Chi Square Test ◽

Polymerase Chain

Abstract Background: The morbidity and mortality of cholangiocarcinoma (CCA) is increasing in recent years. EEF1A2 could regulate multiple biological and pathological processes. Our study was designed to investigate the clinical significance of eEF1A2 for prognosis evaluation in CCA.Methods: The quantitative analysis for eEF1A2 in CCA specimens was performed using quantitative real-time polymerase chain reaction (qRT-PCR). The influences of eEF1A2 expression on disease progression and survival of CCA patients were analyzed by Chi-square test and Kaplan-Meier method, respectively. Additionally, cox regression analyses were adopted to identify the potential predictive biomarker for prognosis of CCA.Results: eEF1A2 expression showed increased tendency in CCA tissues (P<0.05). The expression profile of eEF1A2 was positively correlated with TNM stage (P=0.004), lymph node metastasis (P=0.001) and distant metastasis (P=0.001). In addition, high eEF1A2 expression predicted dismal survival among CCA cases (P<0.05). EEF1A2 might be independently correlated with prognosis of CCA (HR=2.724, 95%CI=1.303-5.964, P=0.008).Conclusions: eEF1A2 may act as a predictive biomarker for clinical outcomes of CCA.

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