scholarly journals Genomic Insights Into Antibiotic Resistance Pattern of Tetracycline Degrading Bacterium Arthrobacter Nicotianae OTC-16

2021 ◽  
Author(s):  
Xin Zhang ◽  
Rongrong Zhu ◽  
Weilin Li ◽  
Junwei Ma ◽  
Hui Lin
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Resistance Pattern ◽  
Streptomycin Sulphate ◽  
Antibiotic Resistance Pattern ◽  
Degrading Bacterium ◽  
Behavioural Characteristics ◽  
Degrading Bacteria ◽  
Arthrobacter Nicotianae ◽  
Polymerase Chain

Abstract Although many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC 16, which shows substantial biodegradation of oxytetracycline/tetracycline. The results showed that this strain could be resistant to at least seven categories, 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC 16 contains one chromosome(3,643,989 bp) and two plasmids (plasmid1,123,894 bp and plasmid2, 29,841bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During oxytetracycline (OTC) degradation by the strain OTC 16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene(tetV) in the chromosome were tracked by real time quantitative reverse transcription polymerase chain reaction(qRT PCR). Only the plasmid derived resistance genes were up regulated under the pressure of OTC. The presence of OTC increased the tolerance of strain OTC 16 to streptomycin sulphate. This work deepens our understanding of the behavioural characteristics of resistance genes and adaptive evolution of resistant drug bacteria under the pressure of antibiotics.

scholarly journals Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin Zhang ◽  
Rongrong Zhu ◽  
Weilin Li ◽  
Junwei Ma ◽  
Hui Lin
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Antibiotic Resistance Pattern ◽  
Degrading Bacterium ◽  
Drug Resistant Bacteria ◽  
Behavioural Characteristics ◽  
Degrading Bacteria ◽  
Arthrobacter Nicotianae

AbstractAlthough many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic-degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC-16, which shows substantial biodegradation of oxytetracycline (OTC)/tetracycline. The results showed that this strain could be resistant to at least seven categories of 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC-16 contains one chromosome (3,643,989 bp) and two plasmids (plasmid1, 123,894 bp and plasmid2, 29,841 bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During OTC degradation by the strain OTC-16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene (tetV) in the chromosome were tracked using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only the plasmid-derived resistance genes were up-regulated in the presence of OTC. The presence of OTC increased the tolerance of strain OTC-16 to streptomycin sulphate. The findings of this study can help deepen our understanding of the behavioural characteristics of resistance genes and adaptive evolution of drug-resistant bacteria.

Multidrug-Resistant Salmonella Typhimurium DT104 in Poultry

2000 ◽  
Vol 63 (2) ◽  
pp. 155-161 ◽  
Author(s):  
G. RAJASHEKARA ◽  
E. HAVERLY ◽  
D. A. HALVORSON ◽  
K. E. FERRIS ◽  
D. C. LAUER ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Phage Type ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Feed Ingredients ◽  
Antibiotic Resistance Pattern ◽  
Phage Types ◽  
Polymerase Chain ◽  
Geographical Locations

Salmonella Typhimurium isolates from feed ingredients or poultry sources isolated during 1995 to 1997 from different geographical locations within Minnesota were examined for the presence of Salmonella Typhimurium definitive type 104 (DT104). Antibiotic susceptibility studies indicated that 15 of 50 isolates of Salmonella Typhimurium had an antibiotic resistance pattern (ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline) that is usually observed with multidrug-resistant Salmonella Typhimurium DT104. Of the 15 isolates showing the antibiotic resistance pattern, 8 isolates were phage type 104, 3 isolates were typed as phage type 104 complex, and the remaining 4 isolates belonged to phage types 193, 81, and 126. DT104 was recovered from both feed ingredients and poultry samples. Of the seven feed ingredient–associated Salmonella Typhimurium isolates, four were DT104, whereas only 7 of 43 poultry-associated Salmonella Typhimurium isolates were DT104. A repetitive sequence–based polymerase chain reaction (rep-PCR) of 50 isolates of Salmonella Typhimurium representing 13 phage types identified seven distinct fingerprint profiles. No correlation between phage type and rep-PCR type was noticed. Eleven Salmonella Typhimurium isolates belonging to DT104 and its complex were grouped into two closely related rep-PCR types.

scholarly journals Presence of blaCTX-M antibiotic resistance gene in Lactobacillus spp. isolated from Hirschsprung diseased infants with stoma

2019 ◽  
Vol 13 (05) ◽  
pp. 426-433 ◽  
Author(s):  
Umama Khan ◽  
Sadia Afsana ◽  
Maria Kibtia ◽  
Mahboob Hossain ◽  
Naiyyum Choudhury ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Antibiotic Resistance Gene ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Antibiotic Resistance Pattern ◽  
Lactobacillus Spp

Introduction: Although antibiotics have revolutionized health care by saving lives, the evolution of both pathogenic and commensal antibiotic-resistant bacteria are emerging as a threat in the health sector. As for Lactobacillus spp., it is usually a non-pathogenic bacteria. However, it can cause infection in immunocompromised condition. In this study, Lactobacillus spp. has been isolated from the faeces of infants with Hirschsprung disease (HD), which is congenital aganglionosis of intestine, where surgical approach and antibiotics are frequently used as medical intervention. The aim of this study is to assess the antibiotic resistance pattern and determine the presence of resistance genes, if any, in Lactobacillus spp. isolated from HD infants with ileostomy. Methodology: Six Lactobacillus spp. were isolated from faeces of six HD infants and confirmed using both conventional and molecular methods. Antibiotic resistance pattern was checked through disc diffusion method and was further investigated for the presence of antibiotic resistance genes (blaTEM, blaCTX-M, blaOXA-2, blaIMP, blaVIM-2, blaNDM-1 and mcr-1). Results: Antibiotic susceptibility of the isolates showed high level of resistance towards cephalosporins, oxacillin, aztreonam, meropenem and polymyxin group. However, four of the isolates showed the presence of blaCTX-M gene after PCR amplification. Conclusions: To our knowledge, this is the first report on the presence of antibiotic resistance gene blaCTX-M in Lactobacillus spp. and this presence may pose a serious threat in treatment regimen. As not much is known regarding the presence of blaCTX-M in Lactobacillus spp., this finding may provide new light to research on antibiotic resistance in gut microflora.

Molecular Characterization and Antibiotic Resistance Pattern of Isolated Acinetobacter baumannii in Iran

Gene Reports ◽  
2021 ◽  
pp. 101195
Author(s):  
Hossein Masoumi-Asl ◽  
Fatemah Sadeghpour Heravi ◽  
Ali Badamchi ◽  
Khadijeh Khanaliha ◽  
Marziye Farsimadan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Resistance Pattern ◽  
Antibiotic Resistance Pattern

scholarly journals Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Grab Sampling ◽  
Antibiotic Resistance Pattern ◽  
Antibiotic Susceptibility Test ◽  
Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 

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