Enhanced Drought Tolerance and Yield in Cry1ac and APX Co-Transformed Transgenic Rice (Oryza Sativa L.)

2021 ◽  
Author(s):  
Vinona Deepthi CH ◽  
Jalaja N
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzymes ◽  
Abiotic Stress ◽  
Transgenic Plants ◽  
Reducing Sugars ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Ms Medium ◽  
Molecular Analyses ◽  
Transgenic Lines

Abstract In the present study, transgenic cry1Ac-APX plants were developed in popular rice variety, BPT 5204, employing Agrobacterium LBA4404 harbouring pcam-cry1Ac-APX vector. The transgenic plants generated were analysed based on herbicide (Basta) toleranceand molecular analyses. Seeds of T1 generation when germinated on MS medium containing 4 mg/l phosphinothricin (ppt) revealed 3 tolerant: 1 susceptible plants suggesting that the trangenes showed monogenic segregation. Homozygous lines were identified by 100% germination of T3 seed on PPT containing media. Abiotic stress assays of analysis cry1Ac-APX transgenic lines showed enhanced chlorophyll, proline and reducing sugars compared to untransformed control plants. The antioxidant enzymes SOD and CAT showed significantly higher levels in transgenic lines than UC.RT- PCR analyses revelled increased expression levels of drought related genes OsDREB, OsMYB and bZIP under both stressed and without stress conditions. Further, the pcam-cry1Ac-APX lines exhibited enhanced biomass and yield than UC.

scholarly journals Molecular Analyses of the Metallothionein Gene Family in Rice (Oryza sativa L.)

BMB Reports ◽  
2006 ◽  
Vol 39 (5) ◽  
pp. 595-606 ◽  
Author(s):  
Gongke Zhou ◽  
Yufeng Xu ◽  
Ji Li ◽  
Lingyan Yang ◽  
Jin-Yuan Liu
Keyword(s):  
Oryza Sativa ◽  
Gene Family ◽  
Oryza Sativa L ◽  
Molecular Analyses ◽  
Metallothionein Gene

Functional characterisation of OsAMT1.1 overexpression lines of rice, Oryza sativa

2006 ◽  
Vol 33 (4) ◽  
pp. 339 ◽  
Author(s):  
Anshuman Kumar ◽  
Brent N. Kaiser ◽  
M. Yaeesh Siddiqi ◽  
Anthony D. M. Glass
Keyword(s):  
Oryza Sativa ◽  
Plant Biomass ◽  
Oryza Sativa L ◽  
Wild Type ◽  
High Affinity ◽  
Functional Characterisation ◽  
Overexpression Line ◽  
Net Uptake ◽  
Transgenic Lines ◽  
Responsive Gene

In rice (Oryza sativa L.) OsAMT1.1 is the most active and / or most N-responsive gene responsible for high-affinity NH4+ transport (HATS) activity. We measured 13NH4+ influx and plant biomass in transgenic overexpression lines and two wild type cultivars of rice, Jarrah and Taipei, with one or more copies of OsAMT1.1. 13NH4+ influx was higher for the overexpression lines of Jarrah line when grown at 10 µm external NH4+ concentration, but not for the overexpression lines of Taipei. For seedlings grown at 2 mm external NH4+ concentration Jarrah lines 77-1 and 75-4 showed an increased influx; however, two overexpression lines of Taipei showed reduced influx rates. The biomasses of the transgenic lines grown at low and high external NH4+ concentrations were either reduced or showed no statistically significant differences compared with wild type lines. While 13NH4+ influx into roots of Jarrah line 75-4 grown at 10 µm external NH4+ concentration was significantly higher than in wild type, measurements of 13NH efflux revealed no differences, and thus net uptake of NH4+ was higher in this overexpression line.

The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.)

2001 ◽  
Vol 158 (6) ◽  
pp. 737-745 ◽  
Author(s):  
Dong Hee Lee ◽  
Young Sang Kim ◽  
Chin Bum Lee
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Oryza Sativa L

A TFIIIA-type zinc finger protein confers multiple abiotic stress tolerances in transgenic rice (Oryza sativa L.)

2012 ◽  
Vol 80 (3) ◽  
pp. 337-350 ◽  
Author(s):  
Ji Huang ◽  
Shujing Sun ◽  
Dongqing Xu ◽  
Hongxia Lan ◽  
Hui Sun ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Abiotic Stress ◽  
Zinc Finger ◽  
Transgenic Rice ◽  
Zinc Finger Protein ◽  
Oryza Sativa L ◽  
Finger Protein

scholarly journals Harnessing the hidden genetic diversity for improving multiple abiotic stress tolerance in rice (Oryza sativa L.)

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0172515 ◽  
Author(s):  
Jauhar Ali ◽  
Jian-Long Xu ◽  
Yong-Ming Gao ◽  
Xiu-Fang Ma ◽  
Li-Jun Meng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Oryza Sativa L ◽  
Abiotic Stress Tolerance

Selenium ameliorates arsenic induced oxidative stress through modulation of antioxidant enzymes and thiols in rice (Oryza sativa L.)

Ecotoxicology ◽  
2014 ◽  
Vol 23 (7) ◽  
pp. 1153-1163 ◽  
Author(s):  
Amit Kumar ◽  
Rana Pratap Singh ◽  
Pradyumna Kumar Singh ◽  
Surabhi Awasthi ◽  
Debasis Chakrabarty ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oryza Sativa ◽  
Antioxidant Enzymes ◽  
Oryza Sativa L

scholarly journals Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

2017 ◽  
Vol 18 (2) ◽  
pp. 91
Author(s):  
Kunti Anis Azizah ◽  
Didik Pudji Restanto ◽  
Bambang Sugiharto
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Medium Composition ◽  
Regeneration Frequency ◽  
Optimum Result

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

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