Functional characterisation of OsAMT1.1 overexpression lines of rice, Oryza sativa

2006 ◽  
Vol 33 (4) ◽  
pp. 339 ◽  
Author(s):  
Anshuman Kumar ◽  
Brent N. Kaiser ◽  
M. Yaeesh Siddiqi ◽  
Anthony D. M. Glass
Keyword(s):  
Oryza Sativa ◽  
Plant Biomass ◽  
Oryza Sativa L ◽  
Wild Type ◽  
High Affinity ◽  
Functional Characterisation ◽  
Overexpression Line ◽  
Net Uptake ◽  
Transgenic Lines ◽  
Responsive Gene

In rice (Oryza sativa L.) OsAMT1.1 is the most active and / or most N-responsive gene responsible for high-affinity NH4+ transport (HATS) activity. We measured 13NH4+ influx and plant biomass in transgenic overexpression lines and two wild type cultivars of rice, Jarrah and Taipei, with one or more copies of OsAMT1.1. 13NH4+ influx was higher for the overexpression lines of Jarrah line when grown at 10 µm external NH4+ concentration, but not for the overexpression lines of Taipei. For seedlings grown at 2 mm external NH4+ concentration Jarrah lines 77-1 and 75-4 showed an increased influx; however, two overexpression lines of Taipei showed reduced influx rates. The biomasses of the transgenic lines grown at low and high external NH4+ concentrations were either reduced or showed no statistically significant differences compared with wild type lines. While 13NH4+ influx into roots of Jarrah line 75-4 grown at 10 µm external NH4+ concentration was significantly higher than in wild type, measurements of 13NH efflux revealed no differences, and thus net uptake of NH4+ was higher in this overexpression line.

scholarly journals Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b-Deficient Rice (Oryza sativa L.)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Minh Khiem Nguyen ◽  
Tin-Han Shih ◽  
Szu-Hsien Lin ◽  
Jun-Wei Lin ◽  
Hoang Chinh Nguyen ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Core Protein ◽  
Profile Analysis ◽  
Oryza Sativa L ◽  
Electron Flow ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Transcription Analysis ◽  
Chl A ◽  
Chl Biosynthesis

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) and wild-type rice (Oryza sativa L.). Chl b-deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11. Two genes encoding PsbR (PSII core protein), FtsZ1, and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b-deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b. This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.

scholarly journals Transcriptome analysis of xa5-mediated resistance to bacterial leaf streak in rice (Oryza sativa L.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiaofang Xie ◽  
Zhiwei Chen ◽  
Binghui Zhang ◽  
Huazhong Guan ◽  
Yan Zheng ◽  
...  
Keyword(s):  
Cell Death ◽  
Molecular Mechanism ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Rna Seq ◽  
Bacterial Leaf Streak ◽  
Wild Type ◽  
Cell Death Pathways ◽  
Rnai Line ◽  
Transgenic Lines

Abstract Bacterial leaf steak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a devastating disease in rice production. The resistance to BLS in rice is a quantitatively inherited trait, of which the molecular mechanism is still unclear. It has been proved that xa5, a recessive bacterial blast resistance gene, is the most possible candidate gene of the QTL qBlsr5a for BLS resistance. To study the molecular mechanism of xa5 function in BLS resistance, we created transgenic lines with RNAi of Xa5 (LOC_Os05g01710) and used RNA-seq to analyze the transcriptomes of a Xa5-RNAi line and the wild-type line at 9 h after inoculation with Xoc, with the mock inoculation as control. We found that Xa5-RNAi could (1) increase the resistance to BLS as expected from xa5; (2) alter (mainly up-regulate) the expression of hundreds of genes, most of which were related to disease resistance; and (3) greatly enhance the response of thousands of genes to Xoc infection, especially of the genes involved in cell death pathways. The results suggest that xa5 is the cause of BLS-resistance of QTL qBlsr5a and it displays BLS resistance effect probably mainly because of the enhanced response of the cell death-related genes to Xoc infection.

The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings

2007 ◽  
Vol 87 (1) ◽  
pp. 49-57 ◽  
Author(s):  
J. Chen ◽  
C. Zhu ◽  
D. Lin ◽  
Z. -X Sun
Keyword(s):  
Lipid Peroxidation ◽  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Wild Type ◽  
Rice Seedlings ◽  
Sensitive Mutant ◽  
Cd Toxicity ◽  
Potential Biomarker

Cadmium-sensitive rice (Oryza sativa L. subsp. Japonica ‘Zhonghua11’) mutants were obtained using an Agrobacterium tumefaciens-based gene delivery system. Significant phenotypic differences were observed between a Cd-sensitive mutant (ST) and wild type (WT) rice seedlings. Results indicated that Cd accumulation in the leaves of the mutant was twice that of the wild type after 10 d of 0.5 mM Cd2+ treatment. Furthermore, a rapid Cd-induced H2O2 increase was observed in the mutant leaves, which induced abnormally early activity in antioxidant enzymes such as superoxide dismutase (SOD). However, the mutant leaves showed lower catalase (CAT) activity. By contrast, guaiacol peroxidase (G-POD) activities were higher in the mutant than in the wild type roots. Together with the Cd toxicity-induced decline of early responsive enzymatic activities in vivo, especially CAT, the inability of mutants to scavenge accumulated H2O2 resulted in higher lipid peroxide levels. H2O2 might also strengthen the expression of G-POD as a signaling molecule. Results suggest that G-POD activity can be a potential biomarker reflecting Cd sensitivity in rice seedlings. Key words: Antioxidant enzyme, Cd toxicity, Cd-sensitive mutant, lipid peroxidation, rice (Oryza sativa L.)

scholarly journals Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice (Oryza sativa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Ouyang ◽  
Xuewu Sun ◽  
Yanning Tan ◽  
Zhizhong Sun ◽  
Dong Yu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Rice ◽  
Oryza Sativa L ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Grain Filling ◽  
Leaf Sheath ◽  
Specific Expression ◽  
Total Soluble Sugar ◽  
Transgenic Lines

Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice (Oryza sativa L.). However, abundant starch particles as an important component of NSCs are still present in the leaf sheath and stem at the late stage of grain filling. There are no studies on how bioengineering techniques can be used to improve the efficiency of NSC remobilization. In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling. RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis. At the same time, the starch and total soluble sugar content in the leaf, stem, and leaf sheath were obviously decreased during the grain-filling period. The photosynthetic rate of transgenic lines was higher than that of the wild types by an average of 4.0 and 9.9%, at 5 and 10 days after flowering, respectively. In addition, the grain-filling rate of transgenic lines was faster than that of the wild types by an average of 26.09%. These results indicate an enhanced transport efficiency of NSCs from source tissues in transgenic rice. Transgenic rice also displayed accelerated leaf senescence, which was hypothesized to contribute to decreased grain weight.

Enhanced Drought Tolerance and Yield in Cry1ac and APX Co-Transformed Transgenic Rice (Oryza Sativa L.)

2021 ◽  
Author(s):  
Vinona Deepthi CH ◽  
Jalaja N
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzymes ◽  
Abiotic Stress ◽  
Transgenic Plants ◽  
Reducing Sugars ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Ms Medium ◽  
Molecular Analyses ◽  
Transgenic Lines

Abstract In the present study, transgenic cry1Ac-APX plants were developed in popular rice variety, BPT 5204, employing Agrobacterium LBA4404 harbouring pcam-cry1Ac-APX vector. The transgenic plants generated were analysed based on herbicide (Basta) toleranceand molecular analyses. Seeds of T1 generation when germinated on MS medium containing 4 mg/l phosphinothricin (ppt) revealed 3 tolerant: 1 susceptible plants suggesting that the trangenes showed monogenic segregation. Homozygous lines were identified by 100% germination of T3 seed on PPT containing media. Abiotic stress assays of analysis cry1Ac-APX transgenic lines showed enhanced chlorophyll, proline and reducing sugars compared to untransformed control plants. The antioxidant enzymes SOD and CAT showed significantly higher levels in transgenic lines than UC.RT- PCR analyses revelled increased expression levels of drought related genes OsDREB, OsMYB and bZIP under both stressed and without stress conditions. Further, the pcam-cry1Ac-APX lines exhibited enhanced biomass and yield than UC.

scholarly journals Expression of Rice (Oryza sativa L.) Genes Involved in High-Affinity Nitrate Transport during the Period of Nitrate Induction

2006 ◽  
Vol 56 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Ryoichi Araki ◽  
Hiroshi Hasegawa
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Nitrate Transport ◽  
High Affinity ◽  
Nitrate Induction

Genetic manipulation of a high-affinity PHR1 target cis-element to improve phosphorous uptake in Oryza sativa L.

2015 ◽  
Vol 87 (4-5) ◽  
pp. 429-440 ◽  
Author(s):  
Wenyuan Ruan ◽  
Meina Guo ◽  
Linlin Cai ◽  
Hongtao Hu ◽  
Changying Li ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Manipulation ◽  
Oryza Sativa L ◽  
Cis Element ◽  
High Affinity ◽  
Phosphorous Uptake

scholarly journals Allele mining for a drought responsive gene DRO1 determining root growth angle in donors of drought tolerance in rice (Oryza sativa L.)

2021 ◽  
Vol 27 (3) ◽  
pp. 523-534
Author(s):  
Bablee Kumari Singh ◽  
M. K. Ramkumar ◽  
Monika Dalal ◽  
Archana Singh ◽  
Amolkumar U. Solanke ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Drought Tolerance ◽  
Root Growth ◽  
Oryza Sativa L ◽  
Allele Mining ◽  
Growth Angle ◽  
Root Growth Angle ◽  
Responsive Gene

scholarly journals Transcriptome Analysis of xa5-mediated Resistance to Bacterial Leaf Streak in Rice (Oryza sativa L.)

2019 ◽  
Author(s):  
Xiaofang Xie ◽  
Zhiwei Chen ◽  
Huazhong Guan ◽  
Yan Zheng ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Cell Death ◽  
Molecular Mechanism ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Rna Seq ◽  
Bacterial Leaf Streak ◽  
Wild Type ◽  
Cell Death Pathways ◽  
Rnai Line ◽  
Transgenic Lines

AbstractBacterial leaf steak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a devastating disease in rice production. The resistance to BLS in rice is a quantitatively inherited trait, of which the molecular mechanism is still unclear. It has been proved that xa5, a recessive bacterial blast resistance gene, is the most possible candidate gene of the QTL qBlsr5a for BLS resistance. To study the molecular mechanism of xa5 function in BLS resistance, we created transgenic lines with RNAi of Xa5 (LOC_Os05g01710) and used RNA-seq to analyze the transcriptomes of a Xa5-RNAi line and the wild-type line at 9 h after inoculation with Xoc, with the mock inoculation with water as control. The results showed that Xa5-RNAi could (1) increase the resistance to BLS as expected from xa5; (2) alter (mainly up-regulate) the expression of hundreds of genes, most of which were related to disease resistance; and (3) greatly enhance the response of thousands of genes to Xoc infection, especially of the genes involved in cell death pathways, suggesting that xa5 displays BLS resistance effect probably mainly because of the enhanced response of the cell death-related genes to Xoc infection.

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